Regev was born in Kiryat Gat in 1965 to Moroccan Jewish immigrant parents. In 1983 she joined the Gadna, where she became a platoon commander, serving in the position until 1986. She has a Master's Degree in Business and a Bachelor's Degree in Informal Education. She is married and has three children.
In November 2008, Regev joined the Likud party, saying that she had been a supporter of the party's platform for many years. She won twenty-seventh place on the party's list for the 2009 elections, just high enough to enter the Knesset as Likud won 27 seats.
In May 2012, Regev participated in an anti-immigration protest and called illegal Sudanese immigrants "a cancer in our body." She later said that the quote was misrepresented, and apologized for seeming to compare human beings to cancer.
Regev was re-elected in the 2015 elections after being placed fifth on the Likud list. She was subsequently appointed Minister of Culture and Sport in the new government.