Flow-Volume loop showing successful FVC maneuver. Positive values represent expiration, negative values represent inspiration. At the start of the test both flow and volume are equal to zero (representing the volume in the spirometer rather than the lung). The trace moves clockwise for expiration followed by inspiration. After the starting point the curve rapidly mounts to a peak (the peak expiratory flow). (Note the FEV1 value is arbitrary in this graph and just shown for illustrative purposes; these values must be calculated as part of the procedure).
|TLC||Total lung capacity: the volume in the lungs at maximal inflation, the sum of VC and RV.|
|TV||Tidal volume: that volume of air moved into or out of the lungs during quiet breathing (VT indicates a subdivision of the lung; when tidal volume is precisely measured, as in gas exchange calculation, the symbol VT or VT is used.)|
|RV||Residual volume: the volume of air remaining in the lungs after a maximal exhalation|
|ERV||Expiratory reserve volume: the maximal volume of air that can be exhaled from the end-expiratory position|
|IRV||Inspiratory reserve volume: the maximal volume that can be inhaled from the end-inspiratory level|
|IC||Inspiratory capacity: the sum of IRV and TV|
|IVC||Inspiratory vital capacity: the maximum volume of air inhaled from the point of maximum expiration|
|VC||Vital capacity: the volume of air breathed out after the deepest inhalation.|
|VT||Tidal volume: that volume of air moved into or out of the lungs during quiet breathing (VT indicates a subdivision of the lung; when tidal volume is precisely measured, as in gas exchange calculation, the symbol VT or VT is used.)|
|FRC||Functional residual capacity: the volume in the lungs at the end-expiratory position|
|RV/TLC%||Residual volume expressed as percent of TLC|
|VA||Alveolar gas volume|
|VL||Actual volume of the lung including the volume of the conducting airway.|
|FVC||Forced vital capacity: the determination of the vital capacity from a maximally forced expiratory effort|
|FEVt||Forced expiratory volume (time): a generic term indicating the volume of air exhaled under forced conditions in the first t seconds|
|FEV1||Volume that has been exhaled at the end of the first second of forced expiration|
|FEFx||Forced expiratory flow related to some portion of the FVC curve; modifiers refer to amount of FVC already exhaled|
|FEFmax||The maximum instantaneous flow achieved during a FVC maneuver|
|FIF||Forced inspiratory flow: (Specific measurement of the forced inspiratory curve is denoted by nomenclature analogous to that for the forced expiratory curve. For example, maximum inspiratory flow is denoted FIFmax. Unless otherwise specified, volume qualifiers indicate the volume inspired from RV at the point of measurement.)|
|PEF||Peak expiratory flow: The highest forced expiratory flow measured with a peak flow meter|
|MVV||Maximal voluntary ventilation: volume of air expired in a specified period during repetitive maximal effort|
Spirometry (meaning the measuring of breath) is the most common of the pulmonary function tests (PFTs), measuring lung function, specifically the amount (volume) and/or speed (flow) of air that can be inhaled and exhaled. Spirometry is an important tool used for generating pneumotachographs, which are helpful in assessing conditions such as asthma, pulmonary fibrosis, cystic fibrosis, and COPD.
- 1 Indications
- 2 Spirometry testing
- 3 Parameters
- 3.1 Forced vital capacity (FVC)
- 3.2 Forced expiratory volume in 1 second (FEV1)
- 3.3 FEV1/FVC ratio (FEV1%)
- 3.4 Forced expiratory flow (FEF)
- 3.5 Forced inspiratory flow 25–75% or 25–50%
- 3.6 Peak expiratory flow (PEF)
- 3.7 Tidal volume (TV)
- 3.8 Total lung capacity (TLC)
- 3.9 Diffusing capacity (DLCO)
- 3.10 Maximum voluntary ventilation (MVV)
- 3.11 Static lung compliance (Cst)
- 3.12 Others
- 4 Technologies used in spirometers
- 5 See also
- 6 References
- 7 Further reading
- 8 External links
Spirometry is indicated for the following reasons:
- to diagnose or manage asthma
- to detect respiratory disease in patients presenting with symptoms of breathlessness, and to distinguish respiratory from cardiac disease as the cause
- to measure bronchial responsiveness in patients suspected of having asthma
- to diagnose and differentiate between obstructive lung disease and restrictive lung disease
- to follow the natural history of disease in respiratory conditions
- to assess of impairment from occupational asthma
- to identify those at risk from pulmonary barotrauma while scuba diving
- to conduct pre-operative risk assessment before anaesthesia or cardiothoracic surgery
- to measure response to treatment of conditions which spirometry detects
- to diagnose the vocal cord dysfunction.
The spirometry test is performed using a device called a spirometer, which comes in several different varieties. Most spirometers display the following graphs, called spirograms:
- a volume-time curve, showing volume (litres) along the Y-axis and time (seconds) along the X-axis
- a flow-volume loop, which graphically depicts the rate of airflow on the Y-axis and the total volume inspired or expired on the X-axis
The basic forced volume vital capacity (FVC) test varies slightly depending on the equipment used.
Generally, the patient is asked to take the deepest breath they can, and then exhale into the sensor as hard as possible, for as long as possible, preferably at least 6 seconds. It is sometimes directly followed by a rapid inhalation (inspiration), in particular when assessing possible upper airway obstruction. Sometimes, the test will be preceded by a period of quiet breathing in and out from the sensor (tidal volume), or the rapid breath in (forced inspiratory part) will come before the forced exhalation.
During the test, soft nose clips may be used to prevent air escaping through the nose. Filter mouthpieces may be used to prevent the spread of microorganisms.
Limitations of test
The maneuver is highly dependent on patient cooperation and effort, and is normally repeated at least three times to ensure reproducibility. Since results are dependent on patient cooperation, FVC can only be underestimated, never overestimated.
Due to the patient cooperation required, spirometry can only be used on children old enough to comprehend and follow the instructions given (6 years old or more), and only on patients who are able to understand and follow instructions — thus, this test is not suitable for patients who are unconscious, heavily sedated, or have limitations that would interfere with vigorous respiratory efforts. Other types of lung function tests are available for infants and unconscious persons.
Another major limitation is the fact that many intermittent or mild asthmatics have normal spirometry between acute exacerbation, limiting spirometry's usefulness as a diagnostic. It is more useful as a monitoring tool: a sudden decrease in FEV1 or other spirometric measure in the same patient can signal worsening control, even if the raw value is still normal. Patients are encouraged to record their personal best measures.
Spirometry can also be part of a bronchial challenge test, used to determine bronchial hyperresponsiveness to either rigorous exercise, inhalation of cold/dry air, or with a pharmaceutical agent such as methacholine or histamine.
Sometimes, to assess the reversibility of a particular condition, a bronchodilator is administered before performing another round of tests for comparison. This is commonly referred to as a reversibility test, or a post bronchodilator test (Post BD), and is an important part in diagnosing asthma versus COPD.
The most common parameters measured in spirometry are Vital capacity (VC), Forced vital capacity (FVC), Forced expiratory volume (FEV) at timed intervals of 0.5, 1.0 (FEV1), 2.0, and 3.0 seconds, forced expiratory flow 25–75% (FEF 25–75) and maximal voluntary ventilation (MVV), also known as Maximum breathing capacity. Other tests may be performed in certain situations.
Results are usually given in both raw data (litres, litres per second) and percent predicted—the test result as a percent of the "predicted values" for the patients of similar characteristics (height, age, sex, and sometimes race and weight). The interpretation of the results can vary depending on the physician and the source of the predicted values. Generally speaking, results nearest to 100% predicted are the most normal, and results over 80% are often considered normal. Multiple publications of predicted values have been published and may be calculated online based on age, sex, weight and ethnicity. However, review by a doctor is necessary for accurate diagnosis of any individual situation.
A bronchodilator is also given in certain circumstances and a pre/post graph comparison is done to assess the effectiveness of the bronchodilator. See the example printout.
Forced vital capacity (FVC)
Forced expiratory volume in 1 second (FEV1)
FEV1 is the volume of air that can forcibly be blown out in one second, after full inspiration. Average values for FEV1 in healthy people depend mainly on sex and age, according to the diagram at left. Values of between 80% and 120% of the average value are considered normal. Predicted normal values for FEV1 can be calculated online and depend on age, sex, height, mass and ethnicity as well as the research study that they are based on.
FEV1/FVC ratio (FEV1%)
FEV1/FVC (FEV1%) is the ratio of FEV1 to FVC. In healthy adults this should be approximately 70–85% (declining with age). In obstructive diseases (asthma, COPD, chronic bronchitis, emphysema) FEV1 is diminished because of increased airway resistance to expiratory flow; the FVC may be decreased as well, due to the premature closure of airway in expiration, just not in the same proportion as FEV1 (for instance, both FEV1 and FVC are reduced, but the former is more affected because of the increased airway resistance). This generates a reduced value (<80%, often ~45%). In restrictive diseases (such as pulmonary fibrosis) the FEV1 and FVC are both reduced proportionally and the value may be normal or even increased as a result of decreased lung compliance.
A derived value of FEV1% is FEV1% predicted, which is defined as FEV1% of the patient divided by the average FEV1% in the population for any person of similar age, sex and body composition.
Forced expiratory flow (FEF)
Forced expiratory flow (FEF) is the flow (or speed) of air coming out of the lung during the middle portion of a forced expiration. It can be given at discrete times, generally defined by what fraction remains of the forced vital capacity (FVC). The usual intervals are 25%, 50% and 75% (FEF25, FEF50 and FEF75), or 25% and 50% of FVC. It can also be given as a mean of the flow during an interval, also generally delimited by when specific fractions remain of FVC, usually 25–75% (FEF25–75%). Average ranges in the healthy population depend mainly on sex and age, with FEF25–75% shown in diagram at left. Values ranging from 50-60% and up to 130% of the average are considered normal. Predicted normal values for FEF can be calculated online and depend on age, sex, height, mass and ethnicity as well as the research study that they are based on.
MMEF or MEF stands for maximal (mid-)expiratory flow and is the peak of expiratory flow as taken from the flow-volume curve and measured in liters per second. It should theoretically be identical to peak expiratory flow (PEF), which is, however, generally measured by a peak flow meter and given in liters per minute.
Recent research suggests that FEF25-75% or FEF25-50% may be a more sensitive parameter than FEV1 in the detection of obstructive small airway disease. However, in the absence of concomitant changes in the standard markers, discrepancies in mid-range expiratory flow may not be specific enough to be useful, and current practice guidelines recommend continuing to use FEV1, VC, and FEV1/VC as indicators of obstructive disease.
More rarely, forced expiratory flow may be given at intervals defined by how much remains of total lung capacity. In such cases, it is usually designated as e.g. FEF70%TLC, FEF60%TLC and FEF50%TLC.
Forced inspiratory flow 25–75% or 25–50%
Forced inspiratory flow 25–75% or 25–50% (FIF 25–75% or 25–50%) is similar to FEF 25–75% or 25–50% except the measurement is taken during inspiration.
Peak expiratory flow (PEF)
Peak expiratory flow (PEF) is the maximal flow (or speed) achieved during the maximally forced expiration initiated at full inspiration, measured in liters per minute or in liters per second.
Tidal volume (TV)
Tidal volume is the amount of air inhaled and exhaled normally at rest
Total lung capacity (TLC)
Total lung capacity (TLC) is the maximum volume of air present in the lungs
Diffusing capacity (DLCO)
Diffusing capacity (or DLCO) is the carbon monoxide uptake from a single inspiration in a standard time (usually 10 seconds). Since air consists of very minute or trace of CO, 10 seconds is considered to be the standard time for inhalation, then rapidly blow it out (exhale). The exhaled gas is tested to determine how much of the tracer gas was absorbed during the breath. This will pick up diffusion impairments, for instance in pulmonary fibrosis. This must be corrected for anemia ; a low hemoglobin concentration, anemia, will reduce DLCO) and pulmonary hemorrhage (excess RBC's in the interstitium or alveoli can absorb CO and artificially increase the DLCO capacity). Atmospheric pressure and/or altitude will also affect measured DLCO, and so a correction factor is needed to adjust for standard pressure. Online calculators are available to correct for hemoglobin levels and altitude and/or pressure where the measurement was taken.
Maximum voluntary ventilation (MVV)
Maximum voluntary ventilation (MVV) is a measure of the maximum amount of air that can be inhaled and exhaled within one minute. For the comfort of the patient this is done over a 15 second time period before being extrapolated to a value for one minute expressed as liters/minute. Average values for males and females are 140–180 and 80–120 liters per minute respectively.
Static lung compliance (Cst)
When estimating static lung compliance, volume measurements by the spirometer needs to be complemented by pressure transducers in order to simultaneously measure the transpulmonary pressure. When having drawn a curve with the relations between changes in volume to changes in transpulmonary pressure, Cst is the slope of the curve during any given volume, or, mathematically, ΔV/ΔP. Static lung compliance is perhaps the most sensitive parameter for the detection of abnormal pulmonary mechanics. It is considered normal if it is 60% to 140% of the average value in the population for any person of similar age, sex and body composition.
In those with acute respiratory failure on mechanical ventilation, "the static compliance of the total respiratory system is conventionally obtained by dividing the tidal volume by the difference between the "plateau" pressure measured at the airway opening (PaO) during an occlusion at end-inspiration and positive end-expiratory pressure (PEEP) set by the ventilator".
|Forced vital capacity (FVC)||4.8 L||3.7 L|
|Tidal volume (Vt)||500 mL||390 mL|
|Total lung capacity (TLC)||6.0 L||4.7 L|
Forced Expiratory Time (FET)
Forced Expiratory Time (FET) measures the length of the expiration in seconds.
Slow vital capacity (SVC)
Slow vital capacity (SVC) is the maximum volume of air that can be exhaled slowly after slow maximum inhalation.
Maximal pressure (Pmax and Pi)
Pmax is the asymptotically maximal pressure that can be developed by the respiratory muscles at any lung volume and Pi is the maximum inspiratory pressure that can be developed at specific lung volumes. This measurement also requires pressure transducers in addition. It is considered normal if it is 60% to 140% of the average value in the population for any person of similar age, sex and body composition. A derived parameter is the coefficient of retraction (CR) which is Pmax/TLC .
Mean transit time (MTT)
Mean transit time is the area under the flow-volume curve divided by the forced vital capacity.
Maximal inspiratory pressure (MIP) MIP, also known as negative inspiratory force (NIF), is the maximum pressure that can be generated against an occluded airway beginning at functional residual capacity (FRC). It is a marker of respiratory muscle function and strength. Represented by centimeters of water pressure (cmH2O) and measured with a manometer. Maximum inspiratory pressure is an important and noninvasive index of diaphragm strength and an independent tool for diagnosing many illnesses. Typical maximum inspiratory pressures in adult males can be estimated from the equation, MIP = 142 - (1.03 x Age) cmH2O, where age is in years.
Technologies used in spirometers
- Volumetric Spirometers
- Water bell
- Bellows wedge
- Flow measuring Spirometers
- Lilly (screen) pneumotach
- Turbine/Stator Rotor (normally incorrectly referred to as a turbine. Actually a rotating vane which spins because of the air flow generated by the subject. The revolutions of the vane are counted as they break a light beam)
- Pitot tube
- Hot-wire anemometer
- American Academy of Allergy, Asthma, and Immunology, "Five Things Physicians and Patients Should Question" (PDF), Choosing Wisely: an initiative of the ABIM Foundation (American Academy of Allergy, Asthma, and Immunology), retrieved 14 August 2012
- Expert Panel Report 3: Guidelines for the Diagnosis and Management of Asthma (PDF) (NIH Publication Number 08-5846 ed.). National Institutes of Health. 2007.
- Bateman, E. D.; Hurd, S. S.; Barnes, P. J.; Bousquet, J.; Drazen, J. M.; Fitzgerald, M.; Gibson, P.; Ohta, K.; O'Byrne, P.; Pedersen, S. E.; Pizzichini, E.; Sullivan, S. D.; Wenzel, S. E.; Zar, H. J. (2008). "Global strategy for asthma management and prevention: GINA executive summary". European Respiratory Journal 31 (1): 143–178. doi:10.1183/09031936.00138707. PMID 18166595.
- Pierce, R. (2005). "Spirometry: An essential clinical measurement". Australian family physician 34 (7): 535–539. PMID 15999163.
- surgeryencyclopedia.com > Spirometry tests. Retrieved 14 March 2010.
- MVV and MBC
- Stanojevic S, Wade A, Stocks J et al. (February 2008). "Reference Ranges for Spirometry Across All Ages: A New Approach". Am. J. Respir. Crit. Care Med. 177 (3): 253–60. doi:10.1164/rccm.200708-1248OC. PMC 2643211. PMID 18006882.
- Perez, LL (March–April 2013). "Office spirometry". Osteopathic Family Physician 5 (2): 65–69. doi:10.1016/j.osfp.2012.09.003.
- LUNGFUNKTION — Practice compendium for semester 6. Department of Medical Sciences, Clinical Physiology, Academic Hospital, Uppsala, Sweden. Retrieved 2010.
- Clinic, the Cleveland (2010). Current clinical medicine 2010 (2nd ed. ed.). Philadelphia, Pa.: Saunders. p. 8. ISBN 1416066438.
- Interpretation model — compendium at Uppsala Academic Hospital. By H. Hedenström. 2009-02-04
- Simon, Michael R.; Chinchilli, Vernon M.; Phillips, Brenda R.; Sorkness, Christine A.; Lemanske Jr., Robert F.; Szefler, Stanley J.; Taussig, Lynn; Bacharier, Leonard B.; Morgan, Wayne (1 September 2010). "Forced expiratory flow between 25% and 75% of vital capacity and FEV1/forced vital capacity ratio in relation to clinical and physiological parameters in asthmatic children with normal FEV1 values". Journal of Allergy and Clinical Immunology 126 (3): 527–534.e8. doi:10.1016/j.jaci.2010.05.016.
- Ciprandi, Giorgio; Cirillo, Ignazio (1 February 2011). "Forced expiratory flow between 25% and 75% of vital capacity may be a marker of bronchial impairment in allergic rhinitis". Journal of Allergy and Clinical Immunology 127 (2): 549–549. doi:10.1016/j.jaci.2010.10.053.
- Pellegrino R, Viegi G, Brusasco V, Crapo RO, Burgos F, Casaburi R, Coates A, van der Grinten CP, Gustafsson P, Hankinson J, Jensen R, Johnson DC, MacIntyre N, McKay R, Miller MR, Navajas D, Pedersen OF, Wanger J (November 2005). "Interpretative strategies for lung function tests". The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology 26 (5): 948–68. doi:10.1183/09031936.05.00035205. PMID 16264058.
- Kreider, Maryl. "Chapter 14.1 Pulmonary Function Testing". ACP Medicine. Decker Intellectual Properties. Retrieved 29 April 2011.
- Nunn AJ, Gregg I (April 1989). "New regression equations for predicting peak expiratory flow in adults". BMJ 298 (6680): 1068–70. doi:10.1136/bmj.298.6680.1068. PMC 1836460. PMID 2497892. Adapted by Clement Clarke for use in EU scale — see Peakflow.com ⇒ Predictive Normal Values (Nomogram, EU scale)
- MedlinePlus Encyclopedia Lung diffusion testing
- George, Ronald B. (2005). Chest medicine: essentials of pulmonary and critical care medicine. Lippincott Williams & Wilkins. p. 96. ISBN 978-0-7817-5273-2.
- Sud, A.; Gupta, D.; Wanchu, A.; Jindal, S. K.; Bambery, P. (2001). "Static lung compliance as an index of early pulmonary disease in systemic sclerosis". Clinical rheumatology 20 (3): 177–180. doi:10.1007/s100670170060. PMID 11434468.
- Rossi A, Gottfried SB, Zocchi L et al. (May 1985). "Measurement of static compliance of the total respiratory system in patients with acute respiratory failure during mechanical ventilation. The effect of intrinsic positive end-expiratory pressure". The American review of respiratory disease 131 (5): 672–7. PMID 4003913.
- Lausted, C.; Johnson, A.; Scott, W.; Johnson, M.; Coyne, K.; Coursey, D. (2006). "Maximum static inspiratory and expiratory pressures with different lung volumes". Biomedical engineering online 5 (1): 29. doi:10.1186/1475-925X-5-29. PMC 1501025. PMID 16677384. 
- Borth, F. M. (1982). "The derivation of an index of ventilatory function from spirometric recordings using canonical analysis". British Journal of Diseases of the Chest 76: 400–756. doi:10.1016/0007-0971(82)90077-8.
- Page 352 in: Irwin, Richard (2008). Procedures, techniques, and minimally invasive monitoring in intensive care medicine. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins. ISBN 078177862X.
- Sachs MC, Enright PL, Hinckley Stukovsky KD, Jiang R, Barr RG, Multi-Ethnic Study of Atherosclerosis Lung Study (2009). "Performance of maximum inspiratory pressure tests and maximum inspiratory pressure reference equations for 4 race/ethnic groups.". Respir Care 54 (10): 1321–8. PMID 19796411.
-  "Predicted normal values for maximal respiratory pressures in caucasian adults and children", SH Wilson, NT Cooke, RHT Edwards, SG Spiro
- Miller MR, Crapo R, Hankinson J, Brusasco V, Burgos F, Casaburi R, Coates A, Enright P, van der Grinten CP, Gustafsson P, Jensen R, Johnson DC, MacIntyre N, McKay R, Navajas D, Pedersen OF, Pellegrino R, Viegi G, Wanger J (July 2005). "General considerations for lung function testing". European Respiratory Journal 26 (1): 153–161. doi:10.1183/09031936.05.00034505. PMID 15994402.
|Wikimedia Commons has media related to Spirometry.|
- Detailed information on spirometric testing, interpretation and physiology at spirxpert.com
- General information on spirometers and spirometry
- American Thoracic Society (ATS)
- European Respiratory Society (ERS)
- General Practice Airways Group