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NAA15

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NAA15
Naa15structure.png
Identifiers
Aliases NAA15, Ga19, NARG1, NAT1P, NATH, TBDN, TBDN100, N(alpha)-acetyltransferase 15, NatA auxiliary subunit, MRD50
External IDs MGI: 1922088 HomoloGene: 14211 GeneCards: NAA15
Gene location (Human)
Chromosome 4 (human)
Chr. Chromosome 4 (human)[1]
Chromosome 4 (human)
Genomic location for NAA15
Genomic location for NAA15
Band 4q31.1 Start 139,301,455 bp[1]
End 139,420,033 bp[1]
RNA expression pattern
PBB GE NARG1 219158 s at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_057175

NM_053089

RefSeq (protein)

NP_476516

n/a

Location (UCSC) Chr 4: 139.3 – 139.42 Mb Chr 3: 51.42 – 51.48 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

N-alpha-acetyltransferase 15, NatA auxiliary subunit also known as gastric cancer antigen Ga19 (GA19), NMDA receptor-regulated protein 1 (NARG1), and Tbdn100 is a protein that in humans is encoded by the NAA15 gene.[5] NARG1 is the auxiliary subunit of the NatA (Nα-acetyltransferase A) complex. This NatA complex can associate with the ribosome and catalyzes the transfer of an acetyl group to the Nα-terminal amino group of proteins as they emerge from the exit tunnel.

Gene and transcripts[edit]

Human NAA15 is located on chromosome 4q31.1 and contains 23 exons. Initially, 2 mRNA species were identified, of size 4.6 and 5.8 kb, both harboring the same open reading frame encoding a putative protein of 866 amino acids (~105 kDa) protein that can be detected in most human adult tissues.[5] According to RefSeq/NCBI, only one human transcript variant exists, although 2 more isoforms are predicted.[6] In addition to full length Naa15, an N-terminally truncated variant of Naa15 (named tubedown-1), Naa15273-865 has been described; however, in mouse only full length Naa15 is widely expressed, whereas smaller transcripts seem to visualized only in heart and testis.[7][8]

In addition to this, a NAA15 gene duplication, NAA16, has been identified, and the encoded protein shares 70% sequence identity to hNaa15 and is expressed in a variety of human cell lines, but is generally less abundant as compared to hNaa15.[9] Three isoforms of Naa16 are validated so far (NCBI RefSeq). Mouse NAA15 is located on chromosome 2 D and contains 20 exons, whereas mouse NAA16 is located on chromosome 14 D3 and consists of 21 exons.

In principle, NatA can assemble from all the Naa10 and Naa15 isoforms in human and mouse, creating a more complex and flexible system for Nα-terminal acetylation as compared to lower eukaryotes.[9][10][11]

Structure[edit]

So far, no X-ray crystal structure of human Naa15 has been published. However, the X-ray crystal structure of the holo-NatA complex (Naa10/Naa15) from S. pombe revealed that Naa15 is composed of 13 conserved helical bundle tetratricopeptide repeat (TPR) motifs and adopts a ring-like topology that wraps around the catalytic subunit of NatA, Naa10.[12] This interaction induces conformational changes in the catalytic center of Naa10 that allows the acetylation of conventional NatA substrates.[12]

Because TPR motifs mediate protein–protein interactions, it has been postulated that this domain may facilitate the interaction with other NatA-binding partners such as the ribosome, Naa50/NatE and the HYPK chaperone.[12] Naa15 harbors a putative NLS between residues 612-628 (KKNAEKEKQQRNQKKKK); however, analysis of the nuclear localization of Naa15 revealed discrepant results.[8][13]

Function[edit]

Naa15, together with its catalytic subunit Naa10, constitutes the evolutionarily conserved NatA (Nα-acetyltransferase A) complex, which acetylates the α-amino group of the first amino acid residue of proteins starting with small side chains like serine, glycine, alanine, threonine and cysteine, after the initiator methionine has been cleaved by methionine aminopeptidases.[13][14][15][16][17][18][19]

Both, Naa15 and Naa16 interact with the ribosome in yeast (via the ribosomal proteins, uL23 and uL29), humans and rat, thereby linking the NatA/Naa10 to the ribosome and facilitating co-translational acetylation of nascent polypeptide chains as they emerges from the exit tunnel.[9][20][21][22][23][24] Furthermore, Naa15 might act as a scaffold for other factors, including the chaperone like protein HYPK (Huntingtin Interacting Protein K) and Naa50, the catalytic acetyltransferase subunit of NatE[21][22][25][26] In S. cerevisiae, NAA15Δ and NAA10Δ knockout cells exhibit the same phenotype, and biochemical data indicate that uncomplexed Naa15 is unstable and gets degraded.[12][27][28][29] Therefore, Naa15 function has been closely linked to the acetyltransferase activity of Naa10 as part of the NatA complex.

NatA may also regulate co-translational protein folding and protein targeting to the endoplasmic reticulum, possibly through competition with SRP and NAC for the same ribosomal binding sites or through yet unknown interference with other ribosome-associated protein biogenesis factors, such as the MetAPs, the chaperones Hsp70/Hsp40, SRP and NAC, which act on newly synthesized proteins as soon as they emerge from the ribosome exit tunnel.[20][23][30][31][32][33][34] However, the exact mechanism of such action is obscure. Apart from this, Naa15 has been linked to many cellular processes, including the maintenance of a healthy retina,[35][36][37] endothelial cell permeability,[37] tumor progression,[5][38] generation and differentiation of neurons[14][39][40] apoptosis[9][41] and transcriptional regulation;[8] however, it is not well understood whether these are NatA-independent or -dependent functions of Naa15.

Disease[edit]

Recently, two damaging de novo NAA15 mutations were reported by exome sequencing in parent-offspring trios with congenital heart disease.[42] Patient 1 harbors a frameshift mutation (p. Lys335fs) and displays heterotaxy (dextrocardia, total anomalous pulmonary venous return, left superior vena cava, hypoplastic TV, double outlet right ventricle, hypoplastic RV, D-transposition of the great arteries, pulmonic stenosis) and hydronephrosis, asplenia, malrotation and abnormal neuro-development, the second patient harbors a nonsense mutation (p.S761X) and displays conotruncal defects (tetralogy of Fallot, single left coronary artery).

Notes[edit]

Published version
  1. ^ Max J Dörfel, Gholson J Lyon (10 August 2015). "The biological functions of Naa10 - From amino-terminal acetylation to human disease". Gene. 567 (2): 103–31. doi:10.1016/J.GENE.2015.04.085. PMC 4461483Freely accessible. PMID 25987439. 


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Further reading[edit]

  • Gendron RL, Good WV, Adams LC, Paradis H (2001). "Suppressed expression of tubedown-1 in retinal neovascularization of proliferative diabetic retinopathy". Invest. Ophthalmol. Vis. Sci. 42 (12): 3000–7. PMID 11687548. 
  • He YG, Xie YF, Chen Y, Qian W, Lai JH, Tan DY (2002). "[Cloning and analysis of a novel gene encoding N-terminal acetyltransferase subunit]". Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao. 34 (3): 353–7. PMID 12019451. 
  • Linē A, Stengrēvics A, Slucka Z, Li G, Jankevics E, Rees RC (2002). "Serological identification and expression analysis of gastric cancer-associated genes". Br. J. Cancer. 86 (11): 1824–30. doi:10.1038/sj.bjc.6600321. PMC 2375403Freely accessible. PMID 12087473. 
  • Norwitz ER, Xu S, Xu J, Spiryda LB, Park JS, Jeong KH, McGee EA, Kaiser UB (2002). "Direct binding of AP-1 (Fos/Jun) proteins to a SMAD binding element facilitates both gonadotropin-releasing hormone (GnRH)- and activin-mediated transcriptional activation of the mouse GnRH receptor gene". J. Biol. Chem. 277 (40): 37469–78. doi:10.1074/jbc.M206571200. PMID 12145309. 
  • Lee DK, Kim BC, Kim IY, Cho EA, Satterwhite DJ, Kim SJ (2002). "The human papilloma virus E7 oncoprotein inhibits transforming growth factor-beta signaling by blocking binding of the Smad complex to its target sequence". J. Biol. Chem. 277 (41): 38557–64. doi:10.1074/jbc.M206786200. PMID 12145312. 
  • Arnesen T, Gromyko D, Horvli O, Fluge Ø, Lillehaug J, Varhaug JE (2006). "Expression of N-acetyl transferase human and human Arrest defective 1 proteins in thyroid neoplasms". Thyroid. 15 (10): 1131–6. doi:10.1089/thy.2005.15.1131. PMID 16279846. 
  • Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.