Protide

From Wikipedia, the free encyclopedia
Jump to navigation Jump to search

The ProTide technology is a prodrug approach used in molecular biology and drug design. It is designed to deliver nucleotide analogues (as monophosphate) into the cell (ProTide: PROdrug + nucleoTIDE). It was discovered by Chris McGuigan (School of Pharmacy and Pharmaceutical Sciences, Cardiff University) in the early 1990s.

General representation of a ProTide

History[edit]

A generic diaryl phosphate pro-drug of AZT

The first breakthroughs in ProTide research was made in 1992, when the McGuigan's group noted the efficacy of aryloxy phosphates and phosphoramidates.[1] In particular, diaryl phosphates were prepared from zidovudine (AZT) using simple phosphorochloridate chemistry. For the first time, the anti-HIV activity of these phosphate derivatives of AZT exceeded that of the parent nucleoside in some cases. Moreover, while AZT was almost inactive (EC50 100µM) in the JM cell line, the substituted diaryl phosphate was 10-times more active (EC50 10µM). At the time, it was considered that JM was AZT-insensitive due to poor phosphorylation. It later emerged that an AZT-efflux pump was the source of this poor AZT sensitivity. However, the conclusion remains valid that the diaryl phosphate was more able to retain activity in the JM cell line, and that this may imply a (small) degree of intracellular phosphate delivery. The electron-withdrawing power of the p-nitro groups and putative enhancements in aryl leaving group ability were suggested as the major driving force of this SAR.

AZT protide

Subsequently, a series of aryloxy phosphoramidates of AZT was prepared with various p-aryl substituents and several aminoacids.[2] Compounds were only studied in the AZT-resistant JM cell line to probe potential (implied) AZT-monophosphate release, and the alanine phosphoramidate emerged as strikingly effective. In HIV-1 infected JM all cultures, AZT was inhibitory at 100µM, while the phenyl methoxy alaninyl phosphoramidate was active at 0.8µM. This was taken as the first evidence of a successful nucleotide delivery. It was also noted that in other series there was a marked preference for alanine over leucine (10-fold) and glycine (>100-fold). Moreover, while electron-withdrawing aryl substitution had been noted to be very effective in the diaryl systems, it was detrimental here. Para fluoro substitution had a slight adventitious effect, but not significantly so, while para-nitro substitution led to a 100-fold loss of activity. In a subsequent studies the range of aryl substituents was extended and compounds studied in true TK+ (thymidine kinase competent) and TK- (thymidine kinase deficient) cell lines. None of the phosphoramidates retained the high (2–4 nM) potency of AZT in TK competent cell lines (CEM and MT-4) against either HIV-1 or HIV-2.[3] However, while AZT lost all of its activity in the TK- deficient cell line CEM/TK-, most of the phosphoramidates retained antiviral activity, thus being ca >10–35-fold more active than AZT in this assay. Again, alanine emerged as an important component, with the glycine analogue being inactive in HIV-infected CEM/TK- all cultures. In this assay, leucine and phenylalanine were as effective as alanine, although they were less so in CEM/TK+ assays. Thus, the parent phenyl methoxy alanyl phosphoramidate emerged as an important lead compound.

Phenyl isoproyl Ala Abacavir monophosphate

Stavudine (d4T) was an early application of the ProTide approach.[4] This was a rational choice based on the known kinetics of phosphorylation of d4T. Thus, while the second phosphorylation (AZT-monophosphate to AZT-diphosphate) but not the first phosphorylation (AZT to AZT-monophosphate) is regarded as rate limiting for AZT activation to the triphosphate, the first step (d4T to d4T monophosphate) is thought in general to be the slow step for d4T. Thus, an intracellular (mono)nucleotide delivery should have maximal impact for d4T and similar nucleosides. In the first instance (halo)alkyloxy phosphoramidates of d4T were prepared and found to retain activity in d4T-resistant JM cells. The activity was dependent on the haloalkyl group; the parent propyl system being poorly active. Subsequent studies in HIV-infected CEM/TK- cell cultures revealed the aryloxy phosphoramidates of d4T to be highly effective and, notably, to retain their full activity in CEM/TK- cells. In this study the benzyl ester emerged as slightly more potent than the parent methyl compound, being almost 10-times more active than d4T in CEM/TK+ assays and thus ca 300-500 fold more active than d4T, in CEM/TK- assays.

Current applications[edit]

Extensive studies followed on these promising d4T derivatives and the ProTide technology was successfully applied to a wide range of nucleoside analogues.[5][6] In particular, the ProTide approach has been used on several clinically evaluated anti-HCV nucleoside analogues, including the 2013 FDA approved compound, sofosbuvir.

Sofosbuvir

References[edit]

  1. ^ McGuigan, C; Pathirana, RN; Mahmood, N; Devine, KG; Hay, AJ (April 1992). "Aryl phosphate derivatives of AZT retain activity against HIV1 in cell lines which are resistant to the action of AZT". Antiviral Research. 17 (4): 311–21. doi:10.1016/0166-3542(92)90026-2. PMID 1642482.
  2. ^ McGuigan, Christopher; Pathirana, Ranjith N.; Mahmood, Naheed; Hay, Alan J. (July 1992). "Aryl phosphate derivates of AZT inhibit HIV replication in cells where the nucleoside is poorly active". Bioorganic & Medicinal Chemistry Letters. 2 (7): 701–704. doi:10.1016/S0960-894X(00)80395-9.
  3. ^ McGuigan, Christopher; Pathirana, Ranjith N.; Balzarini, Jan; De Clercq, Erik (April 1993). "Intracellular delivery of bioactive AZT nucleotides by aryl phosphate derivatives of AZT". Journal of Medicinal Chemistry. 36 (8): 1048–1052. doi:10.1021/jm00060a013.
  4. ^ McGuigan, Christopher; Sheeka, Hendrika M.; Mahmood, Naheed; Hay, Alan (June 1993). "Phosphate derivatives of d4T as inhibitors of HIV". Bioorganic & Medicinal Chemistry Letters. 3 (6): 1203–1206. doi:10.1016/S0960-894X(00)80315-7.
  5. ^ Cahard, D.; McGuigan, C.; Balzarini, J. (2004). "Aryloxy Phosphoramidate Triesters as Pro-Tides". Mini Reviews in Medicinal Chemistry. 4 (4). doi:10.2174/1389557043403936.
  6. ^ McGuigan, Christopher; Harris, Sarah A.; Daluge, Susan M.; Gudmundsson, Kristjan S.; McLean, Ed W.; Burnette, Thimysta C.; Marr, Harry; Hazen, Richard; Condreay, Lynn D.; Johnson, Lance; De Clercq, Erik; Balzarini, Jan (May 2005). "Application of Phosphoramidate Pronucleotide Technology to Abacavir Leads to a Significant Enhancement of Antiviral Potency". Journal of Medicinal Chemistry. 48 (10): 3504–3515. doi:10.1021/jm0491400. PMID 15887959.