Jump to content

Stoma

From Wikipedia, the free encyclopedia

This is an old revision of this page, as edited by Thine Antique Pen (talk | contribs) at 14:36, 7 October 2018 (Reverted edits by 112.79.137.240 (talk): editing tests (HG) (3.4.4)). The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

Not to be confused with Stroma.
For natural and surgically created body openings, see Stoma (medicine).
Stoma in a tomato leaf shown via colorized scanning electron microscope image
A stoma in cross section
The underside of a leaf. In this species (Tradescantia zebrina) the guard cells of the stomata are green because they contain chlorophyll while the epidermal cells are chlorophyll-free and contain red pigments.

In botany, a stoma (plural "stomata"), also called a stomata (plural "stomates")[1] (from Greek στόμα, "mouth"),[2] is a pore, found in the epidermis of leaves, stems, and other organs, that facilitates gas exchange. The pore is bordered by a pair of specialized parenchyma cells known as guard cells that are responsible for regulating the size of the stomatal opening.

The term is usually used collectively to refer to the entire stomatal complex, consisting of the paired guard cells and the pore itself, which is referred to as the stomatal aperture.[3] Air enters the plant through these openings by gaseous diffusion, and contains carbon dioxide and oxygen, which are used in photosynthesis and respiration, respectively. Oxygen produced as a by-product of photosynthesis diffuses out to the atmosphere through these same openings. Also, water vapor diffuses through the stomata into the atmosphere in a process called transpiration.

Stomata are present in the sporophyte generation of all land plant groups except liverworts. In vascular plants the number, size and distribution of stomata varies widely. Dicotyledons usually have more stomata on the lower surface of the leaves than the upper surface. Monocotyledons such as onion, oat and maize may have about the same number of stomata on both leaf surfaces.[4]: 5  In plants with floating leaves, stomata may be found only on the upper epidermis and submerged leaves may lack stomata entirely. Most tree species have stomata only on the lower leaf surface.[5] Leaves with stomata on both the upper and lower leaf are called amphistomatous leaves; leaves with stomata only on the lower surface are hypostomatous, and leaves with stomata only on the upper surface are epistomatous or hyperstomatous.[5] Size varies across species, with end-to-end lengths ranging from 10 to 80 µm and width ranging from a few to 50 µm.[6]

Function

Electron micrograph of a stoma from a Brassica chinensis (Bok Choy) leaf

CO2 gain and water loss

Carbon dioxide, a key reactant in photosynthesis, is present in the atmosphere at a concentration of about 400 ppm. Most plants require the stomata to be open during daytime. The air spaces in the leaf are saturated with water vapour, which exits the leaf through the stomata; this is known as transpiration. Therefore, plants cannot gain carbon dioxide without simultaneously losing water vapour.[7]

Alternative approaches

Ordinarily, carbon dioxide is fixed to ribulose-1,5-bisphosphate (RuBP) by the enzyme RuBisCO in mesophyll cells exposed directly to the air spaces inside the leaf. This exacerbates the transpiration problem for two reasons: first, RuBisCo has a relatively low affinity for carbon dioxide, and second, it fixes oxygen to RuBP, wasting energy and carbon in a process called photorespiration. For both of these reasons, RuBisCo needs high carbon dioxide concentrations, which means wide stomatal apertures and, as a consequence, high water loss.

Narrower stomatal apertures can be used in conjunction with an intermediary molecule with a high carbon dioxide affinity, PEPcase (Phosphoenolpyruvate carboxylase). Retrieving the products of carbon fixation from PEPCase is an energy-intensive process, however. As a result, the PEPCase alternative is preferable only where water is limiting but light is plentiful, or where high temperatures increase the solubility of oxygen relative to that of carbon dioxide, magnifying RuBisCo's oxygenation problem.

CAM plants

C3 and C4 plants(1) stomata stay open all day and close at night. CAM plants(2) stomata open during the morning and close slightly at noon and then open again in the evening.

A group of mostly desert plants called "CAM" plants (Crassulacean acid metabolism, after the family Crassulaceae, which includes the species in which the CAM process was first discovered) open their stomata at night (when water evaporates more slowly from leaves for a given degree of stomatal opening), use PEPcarboxylase to fix carbon dioxide and store the products in large vacuoles. The following day, they close their stomata and release the carbon dioxide fixed the previous night into the presence of RuBisCO. This saturates RuBisCO with carbon dioxide, allowing minimal photorespiration. This approach, however, is severely limited by the capacity to store fixed carbon in the vacuoles, so it is preferable only when water is severely limiting.

Opening and closure

Further information: Guard cell
Opening and closing of stoma.

However, most plants do not have the aforementioned facility and must therefore open and close their stomata during the daytime, in response to changing conditions, such as light intensity, humidity, and carbon dioxide concentration. It is not entirely certain how these responses work. However, the basic mechanism involves regulation of osmotic pressure.

When conditions are conducive to stomatal opening (e.g., high light intensity and high humidity), a proton pump drives protons (H+) from the guard cells. This means that the cells' electrical potential becomes increasingly negative. The negative potential opens potassium voltage-gated channels and so an uptake of potassium ions (K+) occurs. To maintain this internal negative voltage so that entry of potassium ions does not stop, negative ions balance the influx of potassium. In some cases, chloride ions enter, while in other plants the organic ion malate is produced in guard cells. This increase in solute concentration lowers the water potential inside the cell, which results in the diffusion of water into the cell through osmosis. This increases the cell's volume and turgor pressure. Then, because of rings of cellulose microfibrils that prevent the width of the guard cells from swelling, and thus only allow the extra turgor pressure to elongate the guard cells, whose ends are held firmly in place by surrounding epidermal cells, the two guard cells lengthen by bowing apart from one another, creating an open pore through which gas can move.[8]

When the roots begin to sense a water shortage in the soil, abscisic acid (ABA) is released.[9] ABA binds to receptor proteins in the guard cells' plasma membrane and cytosol, which first raises the pH of the cytosol of the cells and cause the concentration of free Ca2+ to increase in the cytosol due to influx from outside the cell and release of Ca2+ from internal stores such as the endoplasmic reticulum and vacuoles.[10] This causes the chloride (Cl−) and organic ions to exit the cells. Second, this stops the uptake of any further K+ into the cells and, subsequently, the loss of K+. The loss of these solutes causes an increase in water potential, which results in the diffusion of water back out of the cell by osmosis. This makes the cell plasmolysed, which results in the closing of the stomatal pores.

Guard cells have more chloroplasts than the other epidermal cells from which guard cells are derived. Their function is controversial.[11][12]

Inferring stomatal behavior from gas exchange

The degree of stomatal resistance can be determined by measuring leaf gas exchange of a leaf. The transpiration rate is dependent on the diffusion resistance provided by the stomatal pores, and also on the humidity gradient between the leaf's internal air spaces and the outside air. Stomatal resistance (or its inverse, stomatal conductance) can therefore be calculated from the transpiration rate and humidity gradient. This allows scientists to investigate how stomata respond to changes in environmental conditions, such as light intensity and concentrations of gases such as water vapor, carbon dioxide, and ozone.[13] Evaporation (E) can be calculated as;[14]

where ei and ea are the partial pressures of water in the leaf and in the ambient air, respectively, P is atmospheric pressure, and r is stomatal resistance. The inverse of r is conductance to water vapor (g), so the equation can be rearranged to;[14]

and solved for g;[14]

Photosynthetic CO2 assimilation (A) can be calculated from

where Ca and Ci are the atmospheric and sub-stomatal partial pressures of CO2, respectively. The rate of evaporation from a leaf can be determined using a photosynthesis system. These scientific instruments measure the amount of water vapour leaving the leaf and the vapor pressure of the ambient air. Photosynthetic systems may calculate water use efficiency (A/E), g, intrinsic water use efficiency (A/g), and Ci. These scientific instruments are commonly used by plant physiologists to measure CO2 uptake and thus measure photosynthetic rate.[15] [16]

Evolution

Tomato stoma observed through immersion oil

There is little evidence of the evolution of stomata in the fossil record, but they had appeared in land plants by the middle of the Silurian period.[17] They may have evolved by the modification of conceptacles from plants' alga-like ancestors.[18] However, the evolution of stomata must have happened at the same time as the waxy cuticle was evolving – these two traits together constituted a major advantage for early terrestrial plants.[citation needed]

Development

There are three major epidermal cell types which all ultimately derive from the outermost (L1) tissue layer of the shoot apical meristem, called protodermal cells: trichomes, pavement cells and guard cells, all of which are arranged in a non-random fashion.

An asymmetrical cell division occurs in protodermal cells resulting in one large cell that is fated to become a pavement cell and a smaller cell called a meristemoid that will eventually differentiate into the guard cells that surround a stoma. This meristemoid then divides asymmetrically one to three times before differentiating into a guard mother cell. The guard mother cell then makes one symmetrical division, which forms a pair of guard cells.[19] Cell division is inhibited in some cells so there is always at least one cell between stomata.[20]

Stomatal patterning is controlled by the interaction of many signal transduction components such as EPF (Epidermal Patterning Factor), ERL (ERecta Like) and YODA (a putative MAP kinase kinase kinase).[20] Mutations in any one of the genes which encode these factors may alter the development of stomata in the epidermis.[20] For example, a mutation in one gene causes more stomata that are clustered together, hence is called Too Many Mouths (TMM).[19] Whereas, disruption of the SPCH (SPeecCHless) gene prevents stomatal development all together.[20]  Activation of stomatal production can occur by the activation of EPF1, which activates TMM/ERL, which together activate YODA. YODA inhibits SPCH, causing SPCH activity to decrease, allowing for asymmetrical cell division that initiates stomata formation.[20][21] Stomatal development is also coordinated by the cellular peptide signal called stomagen, which signals the inhibition of the SPCH, resulting in increased number of stomata.[22]

Environmental and hormonal factors can affect stomatal development. Light increases stomatal development in plants; while, plants grown in the dark have a lower amount of stomata. Auxin represses stomatal development by affecting their development at the receptor level like the ERL and TMM receptors. However, a low concentration of auxin allows for equal division of a guard mother cell and increases the chance of producing guard cells.[23]

Types

Different classifications of stoma types exist. One that is widely used is based on the types that Julien Joseph Vesque introduced in 1889, was further developed by Metcalfe and Chalk,[24] and later complemented by other authors. It is based on the size, shape and arrangement of the subsidiary cells that surround the two guard cells.[25] They distinguish for dicots:

  • actinocytic (meaning star-celled) stomata have guard cells that are surrounded by at least five radiating cells forming a star-like circle. This is a rare type that can for instance be found in the Ebenaceae family.
  • anisocytic (meaning unequal celled) stomata have guard cells between two larger subsidiary cells and one distinctly smaller one. This type of stomata can be found in more than thirty dicot families, including Brassicaceae, Solanaceae, and Crassulaceae. It is sometimes called cruciferous type.
  • anomocytic (meaning irregular celled) stomata have guard cells that are surrounded by cells that have the same size, shape and arrangement as the rest of the epidermis cells. This type of stomata can be found in more than hundred dicot families such as Apocynaceae, Boraginaceae, Chenopodiaceae, and Cucurbitaceae. It is sometimes called ranunculaceous type.
  • diacytic (meaning cross-celled) stomata have guard cells surrounded by two subsidiary cells, that each encircle one end of the opening and contact each other opposite to the middle of the opening. This type of stomata can be found in more than ten dicot families such as Caryophyllaceae and Acanthaceae. It is sometimes called caryophyllaceous type.
  • hemiparacytic stomata are bordered by just one subsidiary cell that differs from the surrounding epidermis cells, its length parallel to the stoma opening. This type occurs for instance in the Molluginaceae and Aizoaceae.
  • paracytic (meaning parallel celled) stomata have one or more subsidiary cells parallel to the opening between the guard cells. These subsidiary cells may reach beyond the guard cells or not. This type of stomata can be found in more than hundred dicot families such as Rubiaceae, Convolvulaceae and Fabaceae. It is sometimes called rubiaceous type.

In monocots, several different types of stomata occur such as:

  • gramineous (meaning grass-like) stomata have two guard cells surrounded by two lens-shaped subsidiary cells. The guard cells are narrower in the middle and bulbous on each end. This middle section is strongly thickened. The axis of the subsidiary cells are parallel stoma opening. This type can be found in monocot families including Poaceae and Cyperaceae.
  • hexacytic (meaning six-celled) stomata have six subsidiary cells around both guard cells, one at either end of the opening of the stoma, one adjoining each guard cell, and one between that last subsidiary cell and the standard epidermis cells. This type can be found in some monocot families.
  • tetracytic (meaning four-celled) stomata have four subsidiary cells, one on either end of the opening, and one next to each guard cell. This type occurs in many monocot families, but also can be found in some dicots, such as Tilia and several Asclepiadaceae.

In ferns, four different types are distinguished:

  • hypocytic stomata have two guard cells in one layer with only ordinary epidermis cells, but with two subsidiary cells on the outer surface of the epidermis, arranged parallel to the guard cells, with a pore between them, overlying the stoma opening.
  • pericytic stomata have two guard cells that are entirely encircled by one continuous subsidiary cell (like a donut).
  • desmocytic stomata have two guard cells that are entirely encircled by one subsidiary cell that has not merged its ends (like a sausage).
  • polocytic stomata have two guard cells that are largely encircled by one subsidiary cell, but also contact ordinary epidermis cells (like a U or horseshoe).

Stomatal crypts

Stomatal crypts are sunken areas of the leaf epidermis which form a chamber-like structure that contains one or more stomata and sometimes trichomes or accumulations of wax. Stomatal crypts can be an adaption to drought and dry climate conditions when the stomatal crypts are very pronounced. However, dry climates are not the only places where they can be found. The following plants are examples of species with stomatal crypts or antechambers: Nerium oleander, conifers, and Drimys winteri which is a species of plant found in the cloud forest.[26]

Stomata as pathogenic pathways

Stomata are obvious holes in the leaf by which, as was presumed for a while, pathogens can enter unchallenged. However, it has been recently shown that stomata do in fact sense the presence of some, if not all, pathogens. However, with the virulent bacteria applied to Arabidopsis plant leaves in the experiment, the bacteria released the chemical coronatine, which forced the stomata open again within a few hours.[27]

Stomata and climate change

Response of stomata to environmental factors

Photosynthesis, plant water transport (xylem) and gas exchange are regulated by stomatal function which is important in the functioning of plants.[28] Stomatal density and aperture (length of stomata) varies under a number of environmental factors such as atmospheric CO2 concentration, light intensity, air temperature and photoperiod (daytime duration). [29][30]

Decreasing stomatal density is one way plants have responded to the increase in concentration of atmospheric CO2 ([CO2]atm).[31] Although changes in [CO2]atm response is the least understood mechanistically, this stomatal response has begun to plateau where it is soon expected to impact transpiration and photosynthesis processes in plants.[28][32]

Future adaptations during climate change

It is expected for [CO2]atm to reach 500–1000 ppm by 2100.[28] 96% of the past 400 000 years experienced below 280 ppm CO2 levels. From this figure, it is highly probable that genotypes of today’s plants diverged from their pre-industrial relative.[28]

The gene HIC (high carbon dioxide) encodes a negative regulator for the development of stomata in plants.[33] Research into the HIC gene using Arabidopsis thaliana found no increase of stomatal development in the dominant allele, but in the ‘wild type’ recessive allele showed a large increase, both in response to rising CO2 levels in the atmosphere.[33] These studies imply the plants response to changing CO2 levels is largely controlled by genetics.

Agricultural implications

In the face of ecological contingencies such as increasing temperatures, changes in rainfall patterns, long term climate change, and biotic influences of human management interventions, it[clarification needed] is expected to reduce the production and quality of food and have a negative impact on agricultural production.[dubious – discuss][failed verification][30][31]

The CO2 fertiliser effect has been greatly overestimated during Free-Air Carbon dioxide Enrichment (FACE) experiments where results show increased CO2 levels in the atmosphere enhances photosynthesis, reduce transpiration, and increase water use efficiency (WUE).[31] Increased biomass is one of the effects with simulations from experiments predicting a 5–20% increase in crop yields at 550 ppm of CO2.[34] Rates of leaf photosynthesis were shown to increase by 30–50% in C3 plants, and 10–25% in C4 under doubled CO2 levels.[34] The existence of a feedback mechanism results a phenotypic plasticity in response to [CO2]atm that may have been an adaptive trait in the evolution of plant respiration and function.[28][30]

Predicting how stomata perform during adaptation is useful for understanding the productivity of plant systems for both natural and agricultural systems.[29] Plant breeders and farmers are beginning to work together using evolutionary and participatory plant breeding to find the best suited species such as heat and drought resistant crop varieties that could naturally evolve to the change in the face of food security challenges.[31]

References

  1. ^ "Living Environment—Regents High school examination" (PDF). January 2011 Regents. NYSED. Retrieved 15 June 2013.
  2. ^ στόμα. Liddell, Henry George; Scott, Robert; A Greek–English Lexicon at the Perseus Project.
  3. ^ Esau, K. (1977). Anatomy of Seed Plants. Wiley and Sons. p. 88. ISBN 0-471-24520-8.
  4. ^ Weyers, J. D. B.; Meidner, H. (1990). Methods in stomatal research. Longman Group UK Ltd. ISBN 0582034833.
  5. ^ a b Willmer, Colin; Fricker, Mark (1996). Stomata. Springer. p. 16. doi:10.1007/978-94-011-0579-8.
  6. ^ Fricker, M.; Willmer, C. (2012). Stomata. Springer Netherlands. p. 18. ISBN 978-94-011-0579-8. Retrieved 15 June 2016.
  7. ^ Debbie Swarthout and C.Michael Hogan. 2010. Stomata. Encyclopedia of Earth. National Council for Science and the Environment, Washington DC
  8. ^ N. S. CHRISTODOULAKIS; J. MENTI; B. GALATIS (January 2002). "Structure and Development of Stomata on the Primary Root of Ceratonia siliqua L." Annals of Botany. 89 (1): 23–29. doi:10.1093/aob/mcf002. PMC 4233769. PMID 12096815.
  9. ^ C. L. Trejo; W. J. Davies; LdMP. Ruiz (1993). "Sensitivity of Stomata to Abscisic Acid (An Effect of the Mesophyll)". Plant Physiology. 102 (2): 497–502. doi:10.1104/pp.102.2.497. PMC 158804. PMID 12231838.
  10. ^ Petra Dietrich; Dale Sanders; Rainer Hedrich (October 2001). "The role of ion channels in light-dependent stomatal opening". Journal of Experimental Botany. 52 (363): 1959–1967. doi:10.1093/jexbot/52.363.1959. PMID 11559731.
  11. ^ "Guard Cell Photosynthesis". Retrieved 2015-10-04.
  12. ^ Eduardo Zeiger; Lawrence D. Talbott; Silvia Frechilla; Alaka Srivastava; Jianxin Zhu (March 2002). "The Guard Cell Chloroplast: A Perspective for the Twenty-First Century". New Phytologist. 153 (3 Special Issue: Stomata): 415–424. doi:10.1046/j.0028-646X.2001.NPH328.doc.x.
  13. ^ Hopkin, Michael (2007-07-26). "Carbon sinks threatened by increasing ozone". Nature. 448 (7152): 396–397. Bibcode:2007Natur.448..396H. doi:10.1038/448396b. PMID 17653153.
  14. ^ a b c "Calculating Important Parameters in Leaf Gas Exchange". Plant Physiology Online. Sinauer. Retrieved 2013-02-24.
  15. ^ Waichi Agata; Yoshinobu Kawamitsu; Susumu Hakoyama; Yasuo Shima (January 1986). "A system for measuring leaf gas exchange based on regulating vapour pressure difference". Photosynthesis Research. 9 (3): 345–357. doi:10.1007/BF00029799. ISSN 1573-5079. PMID 24442366. Retrieved May 6, 2010.
  16. ^ Portable Gas Exchange Fluorescence System GFS-3000. Handbook of Operation (PDF), March 20, 2013
  17. ^ D. Edwards, H. Kerp; Hass, H. (1998). "Stomata in early land plants: an anatomical and ecophysiological approach". Journal of Experimental Botany. 49 (Special Issue): 255–278. doi:10.1093/jxb/49.Special_Issue.255.
  18. ^ Krassilov, Valentin A. (2004). "Macroevolutionary events and the origin of higher taxa". In Wasser, Solomon P. (ed.). Evolutionary theory and processes : modern horizons : papers in honour of Eviatar Nevo. Dordrecht: Kluwer Acad. Publ. pp. 265–289. ISBN 1-4020-1693-X. {{cite book}}: External link in |chapterurl= (help); Unknown parameter |chapterurl= ignored (|chapter-url= suggested) (help)
  19. ^ a b Bergmann, Dominique C.; Lukowitz, Wolfgang; Somerville, Chris R.; Lukowitz, W; Somerville, CR (4 July 2004). "Stomatal Development and Pattern Controlled by a MAPKK Kinase". Science. 304 (5676): 1494–1497. Bibcode:2004Sci...304.1494B. doi:10.1126/science.1096014. PMID 15178800.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  20. ^ a b c d e Pillitteri, Lynn Jo; Dong, Juan (2013-06-06). "Stomatal Development in Arabidopsis". The Arabidopsis Book / American Society of Plant Biologists. 11: e0162. doi:10.1199/tab.0162. ISSN 1543-8120. PMC 3711358. PMID 23864836.
  21. ^ Casson, Stuart A; Hetherington, Alistair M (2010-02-01). "Environmental regulation of stomatal development". Current Opinion in Plant Biology. 13 (1): 90–95. doi:10.1016/j.pbi.2009.08.005.
  22. ^ Sugano, Shigeo S.; Shimada, Tomoo; Imai, Yu; Okawa, Katsuya; Tamai, Atsushi; Mori, Masashi; Hara-Nishimura, Ikuko (2010-01-14). "Stomagen positively regulates stomatal density in Arabidopsis". Nature. 463 (7278): 241–244. Bibcode:2010Natur.463..241S. doi:10.1038/nature08682. ISSN 0028-0836.
  23. ^ Balcerowicz, M.; Ranjan, A.; Rupprecht, L.; Fiene, G.; Hoecker, U. (2014). "Auxin represses stomatal development in dark-grown seedling via Aux/IAA proteins". Development. 141: 3165–3176. doi:10.1242/dev.109181.
  24. ^ Metcalfe, C.R.; Chalk, L. (1950). Anatomy of Dicotyledons. Vol. 1: Leaves, Stem, and Wood in relation to Taxonomy, with notes on economic Uses. {{cite book}}: Cite has empty unknown parameter: |1= (help)
  25. ^ van Cotthem, W.R.F. (1970). "A Classification of Stomatal Types" (PDF). Botanical Journal of the Linnean Society. 63: 235–246. doi:10.1111/j.1095-8339.1970.tb02321.x.
  26. ^ Roth-Nebelsick, A.; Hassiotou, F.; Veneklaas, E. J (2009). "Stomatal crypts have small effects on transpiration: A numerical model analysis". Plant Physiology. 151: 2018–2027. doi:10.1104/pp.109.146969. PMC 2785996.
  27. ^ Maeli Melotto; William Underwood; Jessica Koczan; Kinya Nomura; Sheng Yang He (September 2006). "Plant Stomata Function in Innate Immunity against Bacterial Invasion". Cell. 126 (5): 969–980. doi:10.1016/j.cell.2006.06.054. PMID 16959575.
  28. ^ a b c d e Rico, C; Pittermann, J; Polley, HW; Aspinwall, MJ; Fay, PA (2013). "The effect of subambient to elevated atmospheric CO2 concentration on vascular function in Helianthus annuus: implications for plant response to climate change". New Phytologist. 199: 956–965. doi:10.1111/nph.12339.
  29. ^ a b Buckley, TN; Mott, KA (2013). "Modelling stomatal conductance in response to environmental factors". Plant, Cell and Environment. 36: 1691–1699. doi:10.1111/pce.12140.
  30. ^ a b c Rogiers, SY; Hardie, WJ; Smith, JP (2011). "Stomatal density of grapevine leaves (Vitis Vinifera L.) responds to soil temperature and atmospheric carbon dioxide". Australian Journal of Grape and Wine Research. 17: 147–152. doi:10.1111/j.1755-0238.2011.00124.x.
  31. ^ a b c d Ceccarelli, S; Grando, S; Maatougui, M; Michael, M; Slash, M; Haghparast, R; Rahmanian, M; Taheri, A; Al-Yassin, A; Benbelkacem, A; Labdi, M; Mimoun, H; Nachit, M (2010). "'Plant breeding and climate changes'". The Journal of Agricultural Science. 148 (6): 627–637. doi:10.1017/s0021859610000651.
  32. ^ Serna, L; Fenoll, C (2000). "Coping with human CO2 emissions". Nature. 408: 656–657. doi:10.1038/35047202.
  33. ^ a b Gray, J; Holroyd, G; van der Lee, F; Bahrami, A; Sijmons, P; Woodward, F; Schuch, W; Hetherington, A (2000). "The HIC signalling pathway links CO2 perception to stomatal development". Nature. 408: 713–716. Bibcode:2000Natur.408..713G. doi:10.1038/35047071.
  34. ^ a b Tubiello, FN; Soussana, J-F; Howden, SM (2007). "Crop and pasture response to climate change". Proceedings of the National Academy of Sciences of the United States of America. 104 (50): 19686–19690. Bibcode:2007PNAS..10419686T. doi:10.1073/pnas.0701728104.
Wikimedia Commons has media related to Stoma.
Retrieved from "https://en.wikipedia.org/w/index.php?title=Stoma&oldid=862913452"