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===Olfactory adult stem cells===
===Olfactory adult stem cells===
Olfactory adult stem cells have been successfully harvested from the human olfactory mucosa cells, which are found in the lining of the nose and are involved in the sense of smell.<ref>{{cite journal | author=Murrell W, Feron F, Wetzig A, Cameron N, Splatt K, Bellette B, Bianco J, Perry C, Lee G and Mackay-Sim A | title=Multipotent stem cells from adult olfactory mucosa | journal=Dev Dyn | year=2005 | volume=233 | issue=2 | pages=496–515 | doi=10.1002/dvdy.20360}} {{Entrez Pubmed|15782416}}</ref> If they are given the right chemical environment these cells have the same ability as embryonic stem cells to develop into many different cell types. Olfactory stem cells hold the potential for therapeutic applications and, in contrast to neural stem cells, can be harvested with ease without harm to the patient. This means they can be easily obtained from all individuals, including older patients who might be most in need of stem cell therapies.
Olfactory adult stem cells have been successfully harvested from the human olfactory mucosa cells, which are found in the lining of the nose and are involved in the sense of smell.<ref>{{cite journal | author=Murrell W, Feron F, Wetzig A, Cameron N, Splatt K, Bellette B, Bianco J, Perry C, Lee G and Mackay-Sim A | title=Multipotent stem cells from adult olfactory mucosa | journal=Dev Dyn | year=2005 | volume=233 | issue=2 | pages=496–515 | doi=10.1002/dvdy.20360}} {{Entrez Pubmed|15782416}}</ref> If they are given the right chemical environment these cells have the same ability as embryonic stem cells to develop into many different cell types. Olfactory stem cells hold the potential for therapeutic applications and, in contrast to neural stem cells, can be harvested with ease without harm to the patient. This means they can be easily obtained from all individuals, including older patients who might be most in need of stem cell therapies.

===Neural crest stem cells===
In additional to epidermal stem cells, [[hair follicle]]s contain a type of cells that represent a remnant of the stem cells of the embryonic [[neural crest]]. Similar cells have been found in the [[gastrointestinal tract]], [[sciatic nerve]], cardiac outflow tract and [[Dorsal root ganglion|spinal]] and [[sympathetic ganglia]]. These cells can generate [[neuron]]s, [[Schwann cell]]s, [[myofibroblast]], [[chondrocyte]]s and [[melanocyte]]s.<ref>{{cite journal |author=Sieber-Blum M, Hu Y |title=Epidermal neural crest stem cells (EPI-NCSC) and pluripotency |journal=Stem Cell Rev |volume=4 |issue=4 |pages=256–60 |year=2008 |month=December |pmid=18712509 |doi=10.1007/s12015-008-9042-0}}</ref><ref>{{cite journal |author=Kruger GM, Mosher JT, Bixby S, Joseph N, Iwashita T, Morrison SJ |title=Neural crest stem cells persist in the adult gut but undergo changes in self-renewal, neuronal subtype potential, and factor responsiveness |journal=Neuron |volume=35 |issue=4 |pages=657–69 |year=2002 |month=August |pmid=12194866}}</ref>


===Testicular cells===
===Testicular cells===

Revision as of 11:41, 16 August 2009

Stem cell division and same. A - stem cells; B - progenitor cell; C - differentiated cell; 1 - symmetric stem cell division; 2 - asymmetric stem cell division; 3 - progenitor division; 4 - terminal differentiation

Adult stem cells are undifferentiated cells, found throughout the body after embryonic development, that multiply by cell division to replenish dying cells and regenerate damaged tissues. Also known as somatic stem cells (from Greek Σωματικóς, meaning of the body), they can be found in juvenile as well as adult animals and humans.

Scientific interest in adult stem cells has centered on their ability to divide or self-renew indefinitely, and generate all the cell types of the organ from which they originate, potentially regenerating the entire organ from a few cells. Unlike embryonic stem cells, the use of adult stem cells in research and therapy is not considered to be controversial as they are derived from adult tissue samples rather than destroyed human embryos. They have mainly been studied in humans and model organisms such as mice and rats.

Properties

Defining properties

The rigorous definition of a stem cell requires that it possesses two properties:

  • Self-renewal which is the ability to go through numerous cycles of cell division while maintaining the undifferentiated state.
  • multipotency or multidifferentiative potential which is the ability to generate progeny of several distinct cell types, (for example glial cells and neurons) as opposed to unipotency which is the term for cells that are restricted to producing a single-cell type. However, some researchers do not consider multipotency to be essential, and believe that unipotent self-renewing stem cells can exist.

These properties can be illustrated with relative ease in vitro, using methods such as clonogenic assays, where the progeny of a single cell is characterized, however, it is known that in vitro cell culture conditions can alter the behavior of cells. Proving that a particular subpopulation of cells possesses stem cell properties in vivo is challenging, and so considerable debate exists as to whether some proposed stem cell populations in the adult are indeed stem cells.

Lineage

To ensure self-renewal, stem cells undergo two types of cell division (see Stem cell division and differentiation diagram). Symmetric division gives rise to two identical daughter cells, both endowed with stem cell properties, whereas asymmetric division produces only one stem cell and a progenitor cell with limited self-renewal potential. Asymmetric division is the process of a cell splitting into another cell and an essential cell fat, or a lipid, this lipid will bond to a free cell and reproduce. Progenitors can go through several rounds of cell division before finally differentiating into a mature cell. It is believed that the molecular distinction between symmetric and asymmetric divisions lies in differential segregation of cell membrane proteins (such as receptors) between the daughter cells.

Multidrug resistance

Adult stem cells express transporters of the ATP-binding cassette family that actively pump a diversity of organic molecules out of the cell.[1] Many pharmaceuticals are exported by these transporters conferring multidrug resistance onto the cell. This complicates the design of drugs, for instance neural stem cell targeted therapies for the treatment of clinical depression.

Signaling pathways

Adult stem cell research has been focused on uncovering the general molecular mechanisms that control their self-renewal and differentiation.

  • Bmi-1
The transcriptional repressor Bmi-1 is one of the Polycomb-group proteins that was discovered as a common oncogene activated in lymphoma[2] and later shown to specifically regulate HSCs.[3] The role of Bmi-1 has also been illustrated in neural stem cells.[4]
The Notch pathway has been known to developmental biologists for decades. Its role in control of stem cell proliferation has now been demonstrated for several cell types including haematopoietic, neural and mammary[5] stem cells.
These developmental pathways are also strongly implicated as stem cell regulators.[6]

Plasticity

Under special conditions tissue-specific adult stem cells can generate a whole spectrum of cell types of other tissues, even crossing germ layers.[7] This phenomenon is referred to as stem cell transdifferentiation or plasticity. It can be induced by modifying the growth medium when stem cells are cultured in vitro or transplanting them to an organ of the body different from the one they were originally isolated from. There is yet no consensus among biologists on the prevalence and physiological and therapeutic relevance of stem cell plasticity.

Types

Haematopoietic stem cells

Main article: Pluripotential hemopoietic stem cell

Haematopoietic stem cells are found in the bone marrow and give rise to all the blood cell types.

Mammary stem cells

Mammary stem cells provide the source of cells for growth of the mammary gland during puberty and gestation and play an important role in carcinogenesis of the breast.[8] Mammary stem cells have been isolated from human and mouse tissue as well as from cell lines derived from the mammary gland. Single such cells can give rise to both the luminal and myoepithelial cell types of the gland, and have been shown to have the ability to regenerate the entire organ in mice.[9]

Mesenchymal stem cells

Main article: Mesenchymal stem cell

Mesenchymal stem cells (MSCs) are of stromal origin and may differentiate into a variety of tissues. MSCs have been isolated from placenta, adipose tissue, lung, bone marrow and blood, Wharton's jelly from the umbilical cord,[10] and teeth (perivascular niche of dental pulp and periodontal ligament).[11] MSCs are attractive for clinical therapy due to their ability to differentiate, provide trophic support, and modulate innate immune response.[10]

Endothelial stem cells

Main article: Endothelial stem cell

Neural stem cells

The existence of stem cells in the adult brain has been postulated following the discovery that the process of neurogenesis, the birth of new neurons, continues into adulthood in rats.[12] The presence of stem cells in the mature primate brain was first reported in 1967.[13] It has since been shown that new neurons are generated in adult mice, songbirds and primates, including humans. Normally, adult neurogenesis is restricted to two areas of the brain - the subventricular zone, which lines the lateral ventricles and the dentate gyrus of the hippocampal formation.[14] Although the generation of new neurons in the hippocampus is well established, the presence of true self-renewing stem cells there has been debated.[15] Under certain circumstances, such as following tissue damage in ischemia, neurogenesis can be induced in other brain regions, including the neocortex.

Neural stem cells are commonly cultured in vitro as so called neurospheres - floating heterogeneous aggregates of cells, containing a large proportion of stem cells.[16] They can be propagated for extended periods of time and differentiated into both neuronal and glia cells, and therefore behave as stem cells. However, some recent studies suggest that this behaviour is induced by the culture conditions in progenitor cells, the progeny of stem cell division that normally undergo a strictly limited number of replication cycles in vivo.[17] Furthermore, neurosphere-derived cells do not behave as stem cells when transplanted back into the brain.[18]

Neural stem cells share many properties with haematopoietic stem cells (HSCs). Remarkably, when injected into the blood, neurosphere-derived cells differentiate into various cell types of the immune system.[19]

Olfactory adult stem cells

Olfactory adult stem cells have been successfully harvested from the human olfactory mucosa cells, which are found in the lining of the nose and are involved in the sense of smell.[20] If they are given the right chemical environment these cells have the same ability as embryonic stem cells to develop into many different cell types. Olfactory stem cells hold the potential for therapeutic applications and, in contrast to neural stem cells, can be harvested with ease without harm to the patient. This means they can be easily obtained from all individuals, including older patients who might be most in need of stem cell therapies.

Neural crest stem cells

In additional to epidermal stem cells, hair follicles contain a type of cells that represent a remnant of the stem cells of the embryonic neural crest. Similar cells have been found in the gastrointestinal tract, sciatic nerve, cardiac outflow tract and spinal and sympathetic ganglia. These cells can generate neurons, Schwann cells, myofibroblast, chondrocytes and melanocytes.[21][22]

Testicular cells

Multipotent stem cells with a claimed equivalency to embryonic stem cells have been derived from spermatogonial progenitor cells found in the testicles of laboratory mice by scientists in Germany[23][24][25] and the United States,[26][27][28][29] and, a year later, researchers from Germany and the United Kingdom confirmed the same capability using cells from the testicles of humans.[30] The extracted stem cells are known as human adult germline stem cells (GSCs)[31]

Multipotent stem cells have also been derived from germ cells found in human testicles.[32]

Adult stem cell therapies

Main article: Stem cell treatments

The therapeutic potential of adult stem cells is the focus of much scientific research, due to their ability to be harvested from the patient.[33][34][35] In common with embryonic stem cells, adult stem cells have the ability to differentiate into more than one cell type, but unlike the former they are often restricted to certain types or "lineages". The ability of a differentiated stem cell of one lineage to produce cells of a different lineage is called transdifferentiation. Some types of adult stem cells are more capable of transdifferentiation than others, and for many there is no evidence that such a transformation is possible. Consequently, adult stem therapies require a stem cell source of the specific lineage needed, and harvesting and/or culturing them up to the numbers required is a challenge.[36][37]

Sources

Pluripotent stem cells, i.e. cells that can give rise to any fetal or adult cell type, can be found in a number of tissues, including umbilical cord blood.[38] Using genetic reprogramming, pluripotent stem cells equivalent to embryonic stem cells have been derived from human adult skin tissue.[39][40][41][42][43] Other adult stem cells are multipotent, meaning they are restricted in the types of cell they can become, and are generally referred to by their tissue origin (such as mesenchymal stem cell, adipose-derived stem cell, endothelial stem cell, etc.).[44][45] A great deal of adult stem cell research has focused on investigating their capacity to divide or self-renew indefinitely, and their potential for differentiation.[46] In mice, pluripotent stem cells can be directly generated from adult fibroblast cultures.[47]

Clinical Applications

Adult stem cell treatments have been used for many years to successfully treat leukemia and related bone/blood cancers utilizing bone marrow transplants.[48] The use of adult stem cells in research and therapy is not considered as controversial as the use of embryonic stem cells, because the production of adult stem cells does not require the destruction of an embryo. Consequently, the majority of US government funding provided for research in this field is restricted to supporting adult stem cell research.[49]

Early regenerative applications of adult stem cells has focused on intravenous delivery of blood progenitors known as Hematopetic Stem Cells (HSC's). Other early commercial applications have focused on Mesenchymal Stem Cells (MSC's). For both cell lines, direct injection or placement of cells into a site in need of repair may be the preferred method of treatment, as vascular delivery suffers from a "pulmonary first pass effect" where intravenous injected cells are sequestered in the lungs.[50] Clinical case reports in orthopedic applications have been published. Wakitani has published a small case series of nine defects in five knees involving surgical transplantation of mesenchymal stem cells with coverage of the treated chondral defects.[51] Centeno et al. have reported high field MRI evidence of increased cartilage and meniscus volume in individual human clinical subjects.[52][53] However, Centeno's stem cell clinic is quite controversial and the results have not been published for more treated patients. Many other stem cell based treatments are operating outside the US, with much controversy being reported regarding these treatments as some feel more regulation is needed as clinics tend to exaggerate claims of success and minimize or omit risks.[54]

First transplanted human organ grown from adult stem cells

In 2008 the first full transplant of a human organ grown from adult stem cells was carried out by Paolo Macchiarini, at the Hospital Clínic of Barcelona on Claudia Castillo, a Colombian female adult whose trachea had collapsed due to tuberculosis. Researchers from the University of Padua, the University of Bristol, and Politecnico di Milano harvested a section of trachea from a donor and stripped off the cells that could cause an immune reaction, leaving a grey trunk of cartilage. This section of trachea was then "seeded" with stem cells taken from Ms. Castillo's bone marrow and a new section of trachea was grown in the laboratory over four days. The new section of trachea was then transplanted into the left main bronchus of the patient.[55][56][57][58][59] Because the stem cells were harvested from the patient's own bone marrow Professor Macchiarini did not think it was necessary for her to be given anti-rejection (immunosuppressive) medication and when the procedure was reported four months later in The Lancet, the patient's immune system was showing no signs of rejecting the transplant.[60]

Adult stem cells and cancer

In recent years, acceptance of the concept of adult stem cells has increased. There is now a theory that stem cells reside in many adult tissues and that these unique reservoirs of cells are not only responsible for the normal reparative and regenerative processes, but are also considered to be a prime target for genetic and epigenetic changes, culminating in many abnormal conditions including cancer.[61][62]

News and external links

References

  1. ^ Chaudhary PM and Roninson IB (1991). "Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells". Cell. 66 (1): 85–94. doi:10.1016/0092-8674(91)90141-K. Template:Entrez Pubmed
  2. ^ Haupt Y, Bath ML, Harris AW and Adams JM (1993). "bmi-1 transgene induces lymphomas and collaborates with myc in tumorigenesis". Oncogene. 8: 3161–3164.{{cite journal}}: CS1 maint: multiple names: authors list (link) Template:Entrez Pubmed
  3. ^ Park IK, Qian D, Kiel M, Becker MW, Pihalja M, Weissman IL, Morrison SJ and Clarke MF (2003). "Bmi-1 is required for maintenance of adult self-renewing haematopoietic stem cells". Nature. 423: 302–305. doi:10.1038/nature01587.{{cite journal}}: CS1 maint: multiple names: authors list (link) Template:Entrez Pubmed
  4. ^ Molofsky AV, Pardal R, Iwashita T, Park IK, Clarke MF and Morrison SJ (2003). "Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation". Nature. 425: 962–967. doi:10.1038/nature02060.{{cite journal}}: CS1 maint: multiple names: authors list (link) Template:Entrez Pubmed
  5. ^ Dontu G, Jackson KW, McNicholas E, Kawamura MJ, Abdallah WM and Wicha MS (2004). "Role of Notch signaling in cell-fate determination of human mammary stem/progenitor cells". Breast Cancer Res. 6: R605–615. doi:10.1186/bcr920.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link) PMC 1064073
  6. ^ Beachy PA, Karhadkar SS and Berman DM (2004). "Tissue repair and stem cell renewal in carcinogenesis". Nature. 432: 324–331. doi:10.1038/nature03100. Template:Entrez Pubmed
  7. ^ Filip S, English D and Mokry J (2004). "Issues in stem cell plasticity". J Cell Mol Med. 8 (4): 572–577. doi:10.1111/j.1582-4934.2004.tb00483.x. Template:Entrez Pubmed
  8. ^ Liu S, Dontu G and Wicha MS (2005). "Mammary stem cells, self-renewal pathways, and carcinogenesis". Breast Cancer Res. 7 (3): 86–95. doi:10.1186/bcr1021.{{cite journal}}: CS1 maint: unflagged free DOI (link) Template:Entrez Pubmed
  9. ^ Shackleton M, Vaillant F, Simpson KJ, Stingl J, Smyth GK, Asselin-Labat ML, Wu L, Lindeman GJ and Visvader JE (2005). "Mammary stem cells, self-renewal pathways, and carcinogenesis". Breast Cancer Res. 7 (3): 86–95. doi:10.1186/bcr1021.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link) Template:Entrez Pubmed
  10. ^ a b Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair--current views. Stem Cells. 2007 Nov;25(11):2896-902. Epub 2007 Sep 27. Review.
  11. ^ Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S (2005). "The efficacy of mesenchymal stem cells to regenerate and repair dental structures". Orthod Craniofac Res. 8 (3): 191–9. doi:10.1111/j.1601-6343.2005.00331.x. PMID 16022721. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  12. ^ Altman J and Das GD (1965). "Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats". J Comp Neurol. 124 (3): 319–335. doi:10.1002/cne.901240303.Template:Entrez Pubmed
  13. ^ Lewis PD (1968). "Mitotic activity in the primate subependymal layer and the genesis of gliomas". Nature. 217: 974–975.
  14. ^ Alvarez-Buylla A, Seri B, Doetsch F (2002). "Identification of neural stem cells in the adult vertebrate brain". Brain Res Bull. 57 (6): 751–758. doi:10.1016/S0361-9230(01)00770-5.{{cite journal}}: CS1 maint: multiple names: authors list (link) Template:Entrez Pubmed
  15. ^ Bull ND and Bartlett PF (2005). "The adult mouse hippocampal progenitor is neurogenic but not a stem cell". J Neurosci. 25 (47): 10815–10821. doi:10.1523/JNEUROSCI.3249-05.2005. PMID 16306394. Template:Entrez Pubmed
  16. ^ Reynolds BA and Weiss S (1992). "Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system". Science. 255: 1707–1710. doi:10.1126/science.1553558. PMID 1553558. Template:Entrez Pubmed
  17. ^ Doetsch F, Petreanu L, Caille I, Garcia-Verdugo JM and Alvarez-Buylla A (2002). "EGF converts transit-amplifying neurogenic precursors in the adult brain into multipotent stem cells". Neuron. 36 (6): 1021–1034. doi:10.1016/S0896-6273(02)01133-9.{{cite journal}}: CS1 maint: multiple names: authors list (link) Template:Entrez Pubmed
  18. ^ Marshall GP 2nd, Laywell ED, Zheng T, Steindler DA and Scott EW (2006). "In vitro-derived "neural stem cells" function as neural progenitors without the capacity for self-renewal". Stem Cells. 24 (3): 731–738. doi:10.1634/stemcells.2005-0245. PMID 16339644.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: numeric names: authors list (link) Template:Entrez Pubmed
  19. ^ Bjornson CR, Rietze RL, Reynolds BA, Magli MC and Vescovi AL (1999). "Turning brain into blood: a hematopoietic fate adopted by adult neural stem cells in vivo". Science. 283: 534–537. doi:10.1126/science.283.5401.534. PMID 9915700.{{cite journal}}: CS1 maint: multiple names: authors list (link) Template:Entrez Pubmed
  20. ^ Murrell W, Feron F, Wetzig A, Cameron N, Splatt K, Bellette B, Bianco J, Perry C, Lee G and Mackay-Sim A (2005). "Multipotent stem cells from adult olfactory mucosa". Dev Dyn. 233 (2): 496–515. doi:10.1002/dvdy.20360.{{cite journal}}: CS1 maint: multiple names: authors list (link) Template:Entrez Pubmed
  21. ^ Sieber-Blum M, Hu Y (2008). "Epidermal neural crest stem cells (EPI-NCSC) and pluripotency". Stem Cell Rev. 4 (4): 256–60. doi:10.1007/s12015-008-9042-0. PMID 18712509. {{cite journal}}: Unknown parameter |month= ignored (help)
  22. ^ Kruger GM, Mosher JT, Bixby S, Joseph N, Iwashita T, Morrison SJ (2002). "Neural crest stem cells persist in the adult gut but undergo changes in self-renewal, neuronal subtype potential, and factor responsiveness". Neuron. 35 (4): 657–69. PMID 12194866. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  23. ^ "Testicle cells may aid research". BBC. 2006-03-25.
  24. ^ CBS/Associated Press (2006-03-24). "Study: Mice Testes Act Like Stem Cells". CBS.
  25. ^ Rick Weiss (2006-03-25). "Embryonic Stem Cell Success". Washington Post.
  26. ^ "Promising New Source Of Stem Cells: Mouse Testes Produce Wide Range Of Tissue Types". Science Daily. 2007-09-24.
  27. ^ Barbara Miller (2007-09-20). "Testicles yield stem cells in science breakthrough". Australian Broadcasting Corporation.
  28. ^ J.R. Minkel (2007-09-19). "Testes May Prove Fertile Source of Stem Cells". Scientific American.
  29. ^ "Stem Cells in Adult Testes Provide Alternative to Embryonic Stem Cells for Organ Regeneration". Cornell University. 2007-09-20.
  30. ^ Rob Waters (2008-10-08). "Testicle Stem Cells Become Bone, Muscle in German Experiments". Bloomberg.
  31. ^ Nora Schultz (2008-10-09). "A Source of Men's Stem Cells - Stem cells from human testes could be used for personalized medicine". Technology Review.
  32. ^ Maggie Fox (Reuters) (2006-04-02). "U.S. Firm Says It Made Stem Cells From Human Testes". Washington Post. {{cite web}}: |author= has generic name (help)
  33. ^ Adult stem or progenitor cells in treatment for type 1 diabetes: current progress, Can J Surg, Vol. 50, No. 2, April 2007
  34. ^ Stem Cells: A Revolution in Therapeutics—Recent Advances in Stem Cell Biology and Their Therapeutic Applications in Regenerative Medicine and Cancer Therapies, Clinical Pharmacology & Therapeutics (2007) 82, 252–264;
  35. ^ Stem Cells and Their Potential in Cell-Based Cardiac Therapies, Progress in Cardiovascular Diseases Volume 49, Issue 6, May-June 2007, Pages 396-413
  36. ^ Adult stem cell plasticity: Fact or Artifact?, Annual Review of Cell and Developmental Biology Vol. 19: 1-22
  37. ^ Adult Versus Embryonic Stem Cells: Treatments Science 8 June 2007:Vol. 316. no. 5830, pp. 1422 - 1423
  38. ^ Ratajczak MZ, Machalinski B, Wojakowski W, Ratajczak J, Kucia M (2007). "A hypothesis for an embryonic origin of pluripotent Oct-4(+) stem cells in adult bone marrow and other tissues". Leukemia. 21 (5): 860–7. doi:10.1038/sj.leu.2404630. PMID 17344915.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  39. ^ "Me too, too - How to make human embryonic stem cells without destroying human embryos". The Economist. 2007-11-22.
  40. ^ Gina Kolata (2007-11-22). "Man Who Helped Start Stem Cell War May End It". New York Times.
  41. ^ Gina Kolata (2007-11-21). "Scientists Bypass Need for Embryo to Get Stem Cells". New York Times.
  42. ^ Anne McIlroy (2007-11-21). "Stem-cell method hailed as 'massive breakthrough'". Globe and Mail.
  43. ^ Alice Park (2007-11-20). "A Breakthrough on Stem Cells". Time Magazine.
  44. ^ Barrilleaux B, Phinney DG, Prockop DJ, O'Connor KC (2006). "Review: ex vivo engineering of living tissues with adult stem cells". Tissue Eng. 12 (11): 3007–19. doi:10.1089/ten.2006.12.3007. PMID 17518617.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  45. ^ Gimble JM, Katz AJ, Bunnell BA (2007). "Adipose-derived stem cells for regenerative medicine". Circ. Res. 100 (9): 1249–60. doi:10.1161/01.RES.0000265074.83288.09. PMID 17495232.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  46. ^ Gardner RL (2002). "Stem cells: potency, plasticity and public perception". Journal of Anatomy. 200 (3): 277–82. doi:10.1046/j.1469-7580.2002.00029.x. PMID 12033732.
  47. ^ Takahashi K, Yamanaka S (2006). "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors". Cell. 126 (4): 663–76. doi:10.1016/j.cell.2006.07.024. PMID 16904174.
  48. ^ Bone Marrow Transplant Retrieved on 21 November 2008
  49. ^ Stem Cell FAQ 2004 Retrieved on 21 November 2008
  50. ^ >Fischer; et al. ""Pulmonary passage is a major obstacle for intravenous stem cell delivery: The pulmonary first pass effect". {{cite web}}: Explicit use of et al. in: |author= (help)
  51. ^ >Wakatani; et al. ""Increased knee cartilage volume in degenerative joint disease using percutaneously implanted, autologous mesenchymal stem cells"". {{cite web}}: Explicit use of et al. in: |author= (help)
  52. ^ >Centeno; et al. ""Regeneration of meniscus cartilage in a knee treated with percutaneously implanted autologous mesenchymal stem cells"". {{cite web}}: Explicit use of et al. in: |author= (help)
  53. ^ >Centeno; et al. ""Increased knee cartilage volume in degenerative joint disease using percutaneously implanted, autologous mesenchymal stem cells"". {{cite web}}: Explicit use of et al. in: |author= (help)
  54. ^ >PR Newswire. ""The International Society for Stem Cell Research Releases New Guidelines to Shape Future of Stem Cell Therapy Regulation needed as new study reveals clinics exaggerate claims and omit risks"". {{cite web}}: line feed character in |title= at position 111 (help)
  55. ^ "Claudia Castillo: The pioneer's story". The Independent(United Kingdom). 2008-11-19.
  56. ^ Michael Kahn (2008-11-18). "Woman gets first trachea transplant without drugs". Reuters.
  57. ^ Kate Devlin (2008-11-18). "British doctors help perform world's first transplant of a whole organ grown in lab". The Telegraph (United Kingdom).
  58. ^ Tanya Thompson (2008-11-19). "World first as woman gets organ made from stem cells". The Scotsman.
  59. ^ Jeremy Laurance (2008-11-19). "The medical miracle". The Independent(United Kingdom).
  60. ^ Rose, David (19 November 2008). "Claudia Castillo gets windpipe tailor-made from her own stem cells". The Times. The Times Newspapers Ltd. Retrieved 20 November 2008.
  61. ^ Bioinfobank FAQ:Stem cells in adult tissues Retrieved on 21 november 2008
  62. ^ An Overview of Stem Cell Research and Regulatory Issues Mayo Clin Proc. 2003;78:993-1003 Cogle, Guthrie et al. Retrieved on 21 november 2008
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