Chronic wasting disease
Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) of mule deer, white-tailed deer, elk (or "wapiti"), and moose ("elk" in Europe). To date, CWD has only been found in cervids (members of the deer family). First recognized as a clinical "wasting" syndrome in 1967 in mule deer in a wildlife research facility in northern Colorado, USA, it was identified as a TSE in 1978 and has spread to free-ranging and captive populations in 23 US states and 2 Canadian provinces. CWD is typified by chronic weight loss leading to death. There is no known relationship between CWD and any other TSE of animals or people.
Although there have been reports in the popular press of humans being affected by CWD, a study by the CDC suggests that "[m]ore epidemiologic and laboratory studies are needed to monitor the possibility of such transmissions." The epidemiological study further concludes that, "[a]s a precaution, hunters should avoid eating deer and elk tissues known to harbor the CWD agent (e.g., brain, spinal cord, eyes, spleen, tonsils, lymph nodes) from areas where CWD has been identified."
Most cases of CWD occur in adult animals. The disease is progressive and always fatal. The most obvious and consistent clinical sign of CWD is weight loss over time. Behavioral changes also occur in the majority of cases, including decreased interactions with other animals, listlessness, lowering of the head, blank facial expression, repetitive walking in set patterns, and a smell like meat starting to rot. In elk, behavioral changes may also include hyperexcitability and nervousness. Affected animals continue to eat grain but may show decreased interest in hay. Excessive salivation and grinding of the teeth also are observed. Most deer show increased drinking and urination.
The agent responsible for CWD (and other TSEs, such as scrapie and bovine spongiform encephalopathy) is commonly thought to be a prion, an abnormal form of a normal protein, known as prion protein (PrP), most commonly found in the central nervous system (CNS), and is capable of spreading to the peripheral nervous system (PNS), thus infecting meat, or muscle, of deer and elk. The abnormal prion protein infects the host animal by promoting conversion of normal cellular prion protein (PrPC) to the abnormal prion form (PrPCWD). The build-up of PrPCWD in the brain is associated with widespread neurodegeneration. An alternative theory gaining thus far limited acceptance is that Spiroplasma, a bacterium devoid of a conventional cell wall, may be the causative agent. This does not eliminate prions from playing some role, but speculation has suggested prions may even be a byproduct of Spiroplasma.
Research is being conducted to develop live-animal diagnostic tests for CWD. Currently, definitive diagnosis is based on post mortem examination (necropsy) and testing. Gross lesions seen at necropsy reflect the clinical signs of CWD, primarily emaciation. Aspiration pneumonia, which may be the actual cause of death, also is a common finding in animals affected with CWD On microscopic examination, lesions of CWD in the central nervous system resemble those of other TSEs. In addition, scientists use immunohistochemistry to test brain tissue for the presence of the abnormal prion protein to diagnose CWD.
In 2010, a team from New York described detection of PrPSc even when initially present at only one part in a hundred thousand million (10−11) in brain tissue. The method combines amplification with a novel technology called surround optical fiber immunoassay (SOFIA) and some specific antibodies against PrPSc. After amplifying and then concentrating any PrPSc, the samples are labelled with a fluorescent dye using an antibody for specificity and then finally loaded into a microcapillary tube. This tube is placed in a specially constructed apparatus so it is totally surrounded by optical fibres to capture all light emitted once the dye is excited using a laser. The technique allowed detection of PrPSc after many fewer cycles of conversion than others have achieved, substantially reducing the possibility of artefacts, as well as speeding up the assay. The researchers also tested their method on blood samples from apparently healthy sheep that went on to develop scrapie. The animals’ brains were analysed once any symptoms became apparent. The researchers could therefore compare results from brain tissue and blood taken once the animals exhibited symptoms of the diseases, with blood obtained earlier in the animals’ lives, and from uninfected animals. The results showed very clearly that PrPSc could be detected in the blood of animals long before the symptoms appeared. After further development and testing, this method could be of great value in surveillance as a blood or urine-based screening test for CWD.
The origin and mode of transmission of the prions causing CWD is unknown, but recent research indicates that prions can be excreted by deer and elk, and is transmitted by eating grass growing in contaminated soil. Animals born in captivity and those born in the wild have been affected with the disease. Based on epidemiology, transmission of CWD is thought to be lateral (from animal to animal). Maternal transmission may occur, although it appears to be relatively unimportant in maintaining epidemics. Research has recently shown that an infected deer's saliva is able to spread the CWD prions.
In the mid-1980s, CWD was detected in free-ranging deer and elk in contiguous portions of northeastern Colorado and southeastern Wyoming. Soon after diagnosis of the disease as a TSE, Colorado and Wyoming wildlife management agencies stopped the movement of deer and elk from their research facilities; wild cervids have not been translocated from the endemic area. In May 2001, CWD was also found in free-ranging deer in the southwestern corner of Nebraska (adjacent to Colorado and Wyoming) and later in additional areas in western Nebraska. The limited area of northern Colorado, southern Wyoming, and western Nebraska in which free-ranging deer, moose, and/or elk positive for CWD have been found is referred to as the endemic area. The area in 2006 has expanded to six states including parts of eastern Utah, southwestern South Dakota, and northwestern Kansas. There are also non-contiguous (to the endemic area) areas in central Utah and central Nebraska. The limits of the affected areas are not well defined since the disease is at a low incidence and the amount of sampling may not be adequate to detect it. In 2002, CWD was detected in wild deer in south-central Wisconsin and northern Illinois and in an isolated area of southern New Mexico. In 2005, it was found in wild White-tailed deer in New York and in Hampshire County, West Virginia. In 2008, the first confirmed case of CWD in Michigan was discovered in an infected deer on an enclosed deer breeding facility. It is also found in the Canadian provinces of Alberta and Saskatchewan.
CWD has also been diagnosed in farmed elk and deer herds in a number of states and in two Canadian provinces. The first positive farmed elk herd in the United States was detected in 1997 in South Dakota. Since then, additional positive elk herds and farmed White-tailed deer herds have been found in South Dakota (7), Nebraska (4), Colorado (10), Oklahoma (1), Kansas (1), Minnesota (3), Montana (1), Wisconsin (6) and New York (2). As of fall of 2006, four positive elk herds in Colorado and a positive White-tailed deer herd in Wisconsin remain under State quarantine. All of the other herds have been depopulated or have been slaughtered and tested, and the quarantine has been lifted from one herd that underwent rigorous surveillance with no further evidence of disease. CWD also has been found in farmed elk in the Canadian provinces of Saskatchewan and Alberta. A retrospective study also showed that Mule deer exported from Denver to the Toronto Zoo in the 1980s were affected.
Species that have been affected with CWD include elk, mule deer, white-tailed deer, black-tailed deer, and moose. Other ruminant species, including wild ruminants and domestic cattle, sheep, and goats, have been housed in wildlife facilities in direct or indirect contact with CWD-affected deer and elk, with no evidence of disease transmission. However, experimental transmission of CWD into other ruminants by intracranial inoculation does result in disease, suggesting that only a weak molecular species barrier exists. There is ongoing research to further explore the possibility of transmission of CWD to other species.
Food safety concern
Human exposure to CWD-infected cervids in past decades is likely. The highest levels of prion infectivity are present in the central nervous system and lymphatic tissues of CWD-infected cervids; contamination of knives, saws, and muscles with these tissues can easily occur when processing game. Despite the likelihood of exposures, epidemiologic studies of humans living in CWD-endemic areas of Colorado and Wyoming during 1979–2001 have not shown any increases in human TSE cases. Ongoing studies by the Colorado Department of Public Health and Environmental Human Prion Disease Surveillance Program, in conjunction with the University of Colorado School of Medicine, have also concluded that no convincing cases of CWD transmission to humans have been detected in Colorado. However, if CWD in humans appears like a wasting syndrome similar to that observed in the squirrel monkeys in our study, affected persons might receive a diagnosis of a metabolic disorder and never be tested for TSE. Fortunately, additional laboratory data are consistent with the epidemiologic data, and these results support the conclusion that a species barrier protects humans from CWD infection
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- "Chronic Wasting Disease". 2013-05-21. Retrieved 2014-12-05.
|last1=in Authors list (help)
- Belay, E.D.; Maddox, R.A.; Williams, E.S.; Miller, M.W.; Gambetti, P.; Schonberger, L.B. (June 2004). "Chronic Wasting Disease and Potential Transmission to Humans". Emerging Infectious Diseases (CDC) 10 (6): 977–984. doi:10.3201/eid0905.020577. PMC 3323184. PMID 15207045. Retrieved 2008-04-08.
- Bastian, et al. "Spiroplasma spp. from transmissible spongiform encephalopathy brains or ticks induce spongiform encephalopathy in ruminants". Journal of Medical Microbiology. doi:10.1099/jmm.0.47159-0.
- "Detecting Prions in Blood". Microbiology Today.: 195. August 2010. Retrieved 2011-08-21.
- "SOFIA: An Assay Platform for Ultrasensitive Detection of PrPSc in Brain and Blood". SUNY Downstate Medical Center. Retrieved 2011-08-19.
- "Study Shows Prions Stick Around In Certain Soils". Science Daily. September 17, 2003. Retrieved October 23, 2006.
- "New Research Supports Theory That Indirect Transmission Of Chronic Wasting Disease Possible In Mule Deer". Science Daily. May 19, 2004. Retrieved October 23, 2006.
- Mathiason, CK; Powers JG, Dahmes SJ, Osborn DA, Miller KV, Warren RJ, Mason GL, Hays SA, Hayes-Klug J, Seelig DM, Wild MA, Wolfe LL, Spraker TR, Miller MW, Sigurdson CJ, Telling GC, Hoover EA (October 6, 2006). "Infectious prions in the saliva and blood of deer with chronic wasting disease". Science 314 (5796): 133–6. doi:10.1126/science.1132661. PMID 17023660.
- "Chronic Wasting Disease". West Virginia Division of Natural Resources. 2008-08-01.
- "Wasting Disease Confirmed in State". February 13, 2011.
- Belay ED, Maddox RA, Williams ES, Miller MW, Gambetti P, Schonberger LB. Chronic wasting disease and potential transmission to humans. Emerg Infect Dis. 2004;10:977–84.
- Mawhinney S, Pape WJ, Forster JE, Anderson CA, Bosque P, Miller MW. Human prion disease and relative risk associated with chronic wasting disease. Emerg Infect Dis. 2006;12:1527–35.
- Anderson CA, Bosque P, Filley CM, Arciniegas DB, Kleinschmidt-Demasters BK, Pape WJ, Colorado surveillance program for chronic wasting disease transmission to humans: lessons from 2 highly suspicious but negative cases. Arch Neurol. 2007;64:439–41.
- B, Meade-White KD, Miller MW, Barbian KD, Rubenstein R, LaFauci G, et al. Susceptibilities of nonhuman primates to chronic wasting disease., Emerg Infect Dis [serial on the Internet]. 2009 Sep [date cited]. Available from DOI: 10.3201/eid1509.090253
- *This entry incorporates public domain text originally from the Animal and Plant Health Inspection Service, APHIS.
- Chronic Wasting Disease Alliance
- "Chronic Wasting Disease (CWD) of Deer and Elk". Canadian Food Inspection Agency. 2006-10-03. Archived from the original on September 25, 2006. Retrieved October 23, 2006.
- Elk Research Institute
- United States Geological Survey
- United States Department of Agriculture
- Colorado Division of Wildlife
- Illinois Department of Natural Resources
- Minnesota Department of Natural Resources
- Nebraska Game and Parks Commission
- New York State Department of Environmental Conservation
- Wisconsin Department of Natural Resources
- Wyoming Game and Fish
- Wildlife Disease Information Node Chronic Wasting Disease Factsheet and resources
- A case of chronic wasting disease in a captive red deer (Cervus elaphus)