Higher-energy collisional dissociation
Higher-energy collisional dissociation (HCD, formerly higher-energy C-trap dissociation) is a mass spectrometry fragmentation technique specific to the orbitrap mass spectrometer in which fragmentation takes place external to the trap.
In HCD, the ions pass through the C-trap and into the HCD cell, an added octopole collision cell, where dissociation takes place. The ions are then returned to the C-trap before injection into the orbitrap for mass analysis.
Comparison with collision induced dissociation
HCD does not suffer from the low mass cutoff of resonant-excitation collision-induced dissociation (CID), and therefore is useful for isobaric tag–based quantification as reporter ions can be observed.
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