Higher-energy collisional dissociation

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Higher-energy collisional dissociation (HCD, formerly higher-energy C-trap dissociation) is a mass spectrometry fragmentation technique specific to the orbitrap mass spectrometer in which fragmentation takes place external to the trap.[1]


In HCD, the ions pass through the C-trap and into the HCD cell, an added octopole collision cell, where dissociation takes place. The ions are then returned to the C-trap before injection into the orbitrap for mass analysis.

Comparison with collision induced dissociation[edit]

HCD does not suffer from the low mass cutoff of resonant-excitation collision-induced dissociation (CID), and therefore is useful for isobaric tag–based quantification as reporter ions can be observed.

Collision energy[edit]

Despite the name, the collision energy of HCD is typically in the regime of low energy collision induced dissociation (less than 1000 ev).[1][2]


  1. ^ a b Olsen JV, Macek B, Lange O, Makarov A, Horning S, Mann M (September 2007). "Higher-energy C-trap dissociation for peptide modification analysis". Nat. Methods 4 (9): 709–12. doi:10.1038/nmeth1060. PMID 17721543. 
  2. ^ Murray, Kermit K.; Boyd, Robert K.; Eberlin, Marcos N.; Langley, G. John; Li, Liang; Naito, Yasuhide (2013). "Definitions of terms relating to mass spectrometry (IUPAC Recommendations 2013)". Pure and Applied Chemistry 85 (7). doi:10.1351/PAC-REC-06-04-06. ISSN 1365-3075.