Collet-Serra was born in Sant Iscle de Vallalta, Spain. Soon after graduating, Collet-Serra began his career as an editor before moving on to direct music videos and television commercials for companies such as PlayStation, Budweiser, and Verizon.
Collet-Serra made his television directing debut with the ABC pilot The River, a horror drama from Paranormal Activity writer-director Oren Peli. The episode was filmed in Puerto Rico and the next seven episodes were produced in Hawaii. The show was met with generally favorable reviews, with a collective score of 64/100 from Metacritic from 27 critics. Matt Roush of TV Guide called the show "a terrifying heart-stopper, a cleverly cinematic supernatural adventure that takes us on a wild ride into an exotic heart of darkness."USA Today 's Robert Bianco stated: "There are moments when The River, with its curse-around-every-corner setup, threatens to topple over into farce. But a strong cast and that things-that-go-bump-in-the-night shooting style so far keep the show on course." However, Mike Hale of The New York Times rated the show poorly, saying: "The mixture of Lost storytelling and Paranormal Activity style is neither intriguing nor particularly scary, and it doesn't help that there's hardly a glimmer of humor."
Collet-Serra formed his own production house, Ombra Films. With money from StudioCanal, the small company will make low-budget English-language horror movies, with an eye toward launching the careers of up-and-coming Spanish filmmakers. Juan Sola, Collet-Sera's producing partner, told Deadline: "We've seen how many talented directors are coming out of Spain, but there hasn't really been a platform for them to find work in films. They look up to Jaume as a guy they want to become and we thought, how about a company that creates a bridge between Spain and the U.S., similar to what Luc Besson provided in France? Already, we are being bombarded, and we feel directors will find Ombra a fantastic platform. Jaume loves mentoring new talent." The venture's first film was Mindscape, a psychological thriller about a man with the ability to enter people's memories, starring Taissa Farmiga, Mark Strong and Brian Cox. Collet-Serra executive produced the Comedy Found footage horror film Hooked Up, which was directed by Pablo Larcuen and was shot on a IPhone.