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Centrosome

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Schematic of typical animal cell, showing subcellular components.

Organelles:

(1) nucleolus
(2) nucleus
(3) ribosomes (little dots)
(4) vesicle
(5) rough endoplasmic reticulum (ER)
(6) Golgi apparatus
(7) Cytoskeleton
(8) smooth ER
(9) In cell biology, the centrosome is an organelle that serves as the main microtubule organizing center (MTOC) of the animal cell as well as a regulator of cell-cycle progression. It was discovered by Edouard Van Beneden in 1883 [1] and was described and named in 1888 by Theodor Boveri.[2] The centrosome is thought to have evolved only in the metazoan lineage of eukaryotic cells.[3] Fungi and plants use other MTOC structures to organize their microtubules.[4][5] Although the centrosome has a key role in efficient mitosis in animal cells, it is not necessary.[6]

Centrosomes are composed of two orthogonally arranged centrioles surrounded by an amorphous mass of protein termed the pericentriolar material (PCM). The PCM contains proteins responsible for microtubule nucleation and anchoring[7] including γ-tubulin, pericentrin and ninein. In general, each centriole of the centrosome is based on a nine triplet microtubule assembled in a cartwheel structure, and contains centrin, cenexin and tektin.[8]

Roles of the centrosome

Role of the centrosome in cell cycle progression

Centrosomes are often associated with the nuclear membrane during interphase of the cell cycle. In mitosis the nuclear membrane breaks down and the centrosome nucleated microtubules can interact with the chromosomes to build the mitotic spindle.

The mother centriole, the one that was inherited from the mother cell, also has a central role in making cilia and flagella.[8]

The centrosome is copied only once per cell cycle so that each daughter cell inherits one centrosome, containing two centrioles. The centrosome replicates during the S phase of the cell cycle. During the prophase of mitosis, the centrosomes migrate to opposite poles of the cell. The mitotic spindle then forms between the two centrosomes. Upon division, each daughter cell receives one centrosome. Aberrant numbers of centrosomes in a cell have been associated with cancer. Doubling of a centrosome is similar to DNA replication in two respects: the semiconservative nature of the process and the action of cdk2 as a regulator of the process.[9] But the processes are essentially different in that centrosome doubling does not occur by template reading and assembly. The mother centriole just aids in the accumulation of materials required for the assembly of the daughter centriole.[10]

Centrosome (shown by arrow) next to nucleus

Interestingly, centrosomes are not required for the progression of mitosis. When the centrosomes are irradiated by a laser, mitosis proceeds normally with a morphologically normal spindle. Moreover, development of the fruit fly Drosophila is largely normal when centrioles are absent due to a mutation in a gene required for their duplication.[11] In the absence of the centrosome the microtubules of the spindle are focused by motors allowing the formation of a bipolar spindle. Many cells can completely undergo interphase without centrosomes.[8]

Although centrosomes are not required for mitosis or survival of the cell, they are required for survival of the organism. Acentrosomal cells lack radial arrays of astral microtubules. They are also defective in spindle positioning and in ability to establish a central localization site in cytokinesis. The function of centrosome in this context is hypothesized to ensure the fidelity of cell division as it is not necessary but greatly increases the efficacy. Some cell types arrest in the following cell cycle when centrosomes are absent. This is not a universal phenomenon.

When the nematode C. elegans egg is fertilized the sperm delivers a pair of centrioles. These centrioles will form the centrosomes which will direct the first cell division of the zygote and this will determine its polarity. It is not yet clear whether the role of the centrosome in polarity determination is microtubule dependent or independent.

Centrosome Associated Nucleotides

The existence of nucleotides associated with the centrosome remains controversial. Many studies have investigated whether nucleotides associate with the centrosome with varying results.

Recent research in 2006[12] indicates that centrosomes from Surf clam eggs contain RNA sequences. The sequences identified were found in "few to no" other places in the cell, and do not appear in existing genome databases. One identified RNA sequence contains a putative RNA polymerase, leading to the hypothesis of an RNA based genome within the centrosome.

References

  1. ^ Wunderlich, Volker (2002). "JMM - Past and Present". Journal of Molecular Medicine. 80. Springer: 545–548. doi:10.1007/s00109-002-0374-y. PMID 12226736. {{cite journal}}: Unknown parameter |day= ignored (help); Unknown parameter |month= ignored (help); Unknown parameter |trans_title= ignored (|trans-title= suggested) (help)
  2. ^ Boveri, Theodor (1888). Zellen-Studien II: Die Befruchtung und Teilung des Eies von Ascaris megalocephala. Jena: Gustav Fischer Verlag.
  3. ^ Bornens M, Azimzadeh J (2007). "Origin and evolution of the centrosome". Adv. Exp. Med. Biol. 607: 119–29. doi:10.1007/978-0-387-74021-8_10. PMID 17977464.
  4. ^ Schmit AC (2002). "Acentrosomal microtubule nucleation in higher plants". Int. Rev. Cytol. 220: 257–89. doi:10.1016/S0074-7696(02)20008-X. PMID 12224551.
  5. ^ Jaspersen SL, Winey M (2004). "The budding yeast spindle pol body: structure, duplication, and function". Annu. Rev. Cell Dev. Biol. 20: 1–28. doi:10.1146/annurev.cellbio.20.022003.114106. PMID 15473833.
  6. ^ Mahoney NM, Goshima G, Douglass AD, Vale RD (2006). "Making microtubules and mitotic spindles in cells without functional centrosomes". Curr. Biol. 16 (6): 564–9. doi:10.1016/j.cub.2006.01.053. PMID 16546079.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  7. ^ B. Edde, J. Rossier, J.P. Le Caer, E. Desbruyeres, F. Gros & P. Denoulet (1990). "Posttranslational glutamylation of alpha-tubulin". Science. 1990: 83–85. doi:10.1126/science.1967194. PMID 1967194.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  8. ^ a b c Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 11567874, please use {{cite journal}} with |pmid=11567874 instead.
  9. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 11371338, please use {{cite journal}} with |pmid=11371338 instead.
  10. ^ A. Rodrigues-Martins, M. Riparbelli, G. Callaini, D. M. Glover, M. Bettencourt-Dias; Revisiting the Role of the Mother Centriole in Centriole Biogenesis; Science 18 May 2124: Vol. 316. no. 5827, pp. 1046 - 1050; DOI: 10.1126/science.12535925
  11. ^ Attention: This template ({{cite pmid}}) is deprecated. To cite the publication identified by PMID 16814722, please use {{cite journal}} with |pmid=16814722 instead.
  12. ^ Alliegro, M.C., Alliegro, M.A., and R.E. Palazzo. 2006. Proc. Natl. Acad. Sci. USA, 103(24):9034-8 (as reported in Scientific American, p32, August 2006). PMID 16754862
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