In biology, the extracellular matrix (ECM) is a collection of extracellular molecules secreted by cells that provides structural and biochemical support to the surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM.
The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest.
The plant ECM includes cell wall components, like cellulose, in addition to more complex signaling molecules. Some single-celled organisms adopt multicelluar biofilms in which the cells are embedded in an ECM composed primarily of extracellular polymeric substances (EPS).
- 1 Role and importance
- 2 Molecular components
- 3 Mechanical properties of the ECM
- 4 Cell adhesion to the ECM
- 5 Cell types involved in ECM formation
- 6 Extracellular matrix in plants
- 7 Medical applications
- 8 References
- 9 Further reading
Role and importance
Due to its diverse nature and composition, the ECM can serve many functions, such as providing support, segregating tissues from one another, and regulating intercellular communication. The extracellular matrix regulates a cell's dynamic behavior. In addition, it sequesters a wide range of cellular growth factors and acts as a local store for them. Changes in physiological conditions can trigger protease activities that cause local release of such stores. This allows the rapid and local growth factor-mediated activation of cellular functions without de novo synthesis.
Formation of the extracellular matrix is essential for processes like growth, wound healing, and fibrosis. An understanding of ECM structure and composition also helps in comprehending the complex dynamics of tumor invasion and metastasis in cancer biology as metastasis often involves the destruction of extracellular matrix by enzymes such as serine proteases, threonine proteases, and matrix metalloproteinases.
The stiffness and elasticity of the ECM has important implications in cell migration, gene expression, and differentiation. Cells actively sense ECM rigidity and migrate preferentially towards stiffer surfaces in a phenomenon called durotaxis. They also detect elasticity and adjust their gene expression accordingly which has increasingly become a subject of research because of its impact on differentiation and cancer progression.
Components of the ECM are produced intracellularly by resident cells and secreted into the ECM via exocytosis. Once secreted, they then aggregate with the existing matrix. The ECM is composed of an interlocking mesh of fibrous proteins and glycosaminoglycans (GAGs).
GAGs are carbohydrate polymers and are usually attached to extracellular matrix proteins to form proteoglycans (hyaluronic acid is a notable exception, see below). Proteoglycans have a net negative charge that attracts positively charged sodium ions (Na+), which attracts water molecules via osmosis, keeping the ECM and resident cells hydrated. Proteoglycans may also help to trap and store growth factors within the ECM.
Described below are the different types of proteoglycan found within the extracellular matrix.
Heparan sulfate (HS) is a linear polysaccharide found in all animal tissues. It occurs as a proteoglycan (PG) in which two or three HS chains are attached in close proximity to cell surface or ECM proteins. It is in this form that HS binds to a variety of protein ligands and regulates a wide variety of biological activities, including developmental processes, angiogenesis, blood coagulation, and tumour metastasis.
Hyaluronic acid (or "hyaluronan") is a polysaccharide consisting of alternating residues of D-glucuronic acid and N-acetylglucosamine, and unlike other GAGs, is not found as a proteoglycan. Hyaluronic acid in the extracellular space confers upon tissues the ability to resist compression by providing a counteracting turgor (swelling) force by absorbing significant amounts of water. Hyaluronic acid is thus found in abundance in the ECM of load-bearing joints. It is also a chief component of the interstitial gel. Hyaluronic acid is found on the inner surface of the cell membrane and is translocated out of the cell during biosynthesis.
Hyaluronic acid acts as an environmental cue that regulates cell behavior during embryonic development, healing processes, inflammation, and tumor development. It interacts with a specific transmembrane receptor, CD44.
Collagens are the most abundant protein in the ECM. In fact, collagen is the most abundant protein in the human body and accounts for 90% of bone matrix protein content. Collagens are present in the ECM as fibrillar proteins and give structural support to resident cells. Collagen is exocytosed in precursor form (procollagen), which is then cleaved by procollagen proteases to allow extracellular assembly. Disorders such as Ehlers Danlos Syndrome, osteogenesis imperfecta, and epidermolysis bullosa are linked with genetic defects in collagen-encoding genes. The collagen can be divided into several families according to the types of structure they form:
- Fibrillar (Type I, II, III, V, XI)
- Facit (Type IX, XII, XIV)
- Short chain (Type VIII, X)
- Basement membrane (Type IV)
- Other (Type VI, VII, XIII)
Elastins, in contrast to collagens, give elasticity to tissues, allowing them to stretch when needed and then return to their original state. This is useful in blood vessels, the lungs, in skin, and the ligamentum nuchae, and these tissues contain high amounts of elastins. Elastins are synthesized by fibroblasts and smooth muscle cells. Elastins are highly insoluble, and tropoelastins are secreted inside a chaperone molecule, which releases the precursor molecule upon contact with a fiber of mature elastin. Tropoelastins are then deaminated to become incorporated into the elastin strand. Disorders such as cutis laxa and Williams syndrome are associated with deficient or absent elastin fibers in the ECM.
Fibronectins are glycoproteins that connect cells with collagen fibers in the ECM, allowing cells to move through the ECM. Fibronectins bind collagen and cell-surface integrins, causing a reorganization of the cell's cytoskeleton to facilitate cell movement. Fibronectins are secreted by cells in an unfolded, inactive form. Binding to integrins unfolds fibronectin molecules, allowing them to form dimers so that they can function properly. Fibronectins also help at the site of tissue injury by binding to platelets during blood clotting and facilitating cell movement to the affected area during wound healing.
Laminins are proteins found in the basal laminae of virtually all animals. Rather than forming collagen-like fibers, laminins form networks of web-like structures that resist tensile forces in the basal lamina. They also assist in cell adhesion. Laminins bind other ECM components such as collagens, nidogens, and entactins.
Mechanical properties of the ECM
Stiffness and elasticity
The ECM can exist in varying degrees of stiffness and elasticity, from soft brain tissues to hard bone tissues, the elasticity of the ECM can differ by several orders of magnitude. This property is primarily dependent on collagen and elastin concentration, and it has recently been shown to play an influential role in regulating numerous cell functions.
Cells can sense the mechanical properties of their environment by applying forces and measuring the resulting backlash. This plays an important role because it helps regulate many important cellular processes including cellular contraction, cell migration, cell proliferation, differentiation and cell death (apoptosis). Inhibition of nonmuscle myosin II blocks most of these effects, indicating that they are indeed tied to sensing the mechanical properties of the ECM, which has become a new focus in research during the past decade.
Effect on gene expression
Differing mechanical properties in ECM exert effects on both cell behaviour and gene expression. Although the mechanism by which this is done has not been thoroughly explained, adhesion complexes and the actin-myosin cytoskeleton, whose contractile forces are transmitted through transcellular structures are thought to play key roles in the yet to be discovered molecular pathways.
Effect on differentiation
ECM elasticity can direct cellular differentiation, the process by which a cell changes from one cell type to another. In particular, naive mesenchymal stem cells (MSCs) have been shown to specify lineage and commit to phenotypes with extreme sensitivity to tissue-level elasticity. MSCs placed on soft matrices that mimic brain differentiate into neuron-like cells, showing similar shape, RNAi profiles, cytoskeletal markers, and transcription factor levels. Similarly stiffer matrices that mimic muscle are myogenic, and matrices with stiffnesses that mimic collagenous bone are osteogenic.
Stiffness and elasticity also guide cell migration, this process is called durotaxis. The term was coined by Lo CM and colleagues when they discovered the tendency of single cells to migrate up rigidity gradients (towards more stiff substrates) and has been extensively studied since. The molecular mechanisms behind durotaxis are thought to exist primarily in the focal adhesion, a large protein complex that acts as the primary site of contact between the cell and the ECM. This complex contains many proteins that are essential to durotaxis including structural anchoring proteins (integrins) and signaling proteins (adhesion kinase (FAK), talin, vinculin, paxillin, α-actinin, GTPases etc.) which cause changes in cell shape and actomyosin contractility. These changes are thought to cause cytoskeletal rearrangements in order to facilitate directional migration.
Cell adhesion to the ECM
Many cells bind to components of the extracellular matrix. Cell adhesion can occur in two ways; by focal adhesions, connecting the ECM to actin filaments of the cell, and hemidesmosomes, connecting the ECM to intermediate filaments such as keratin. This cell-to-ECM adhesion is regulated by specific cell-surface cellular adhesion molecules (CAM) known as integrins. Integrins are cell-surface proteins that bind cells to ECM structures, such as fibronectin and laminin, and also to integrin proteins on the surface of other cells.
Fibronectins bind to ECM macromolecules and facilitate their binding to transmembrane integrins. The attachment of fibronectin to the extracellular domain initiates intracellular signalling pathways as well as association with the cellular cytoskeleton via a set of adaptor molecules such as actin.
Cell types involved in ECM formation
There are many cell types that contribute to the development of the various types of extracellular matrix found in plethora of tissue types. The local components of ECM determine the properties of the connective tissue.
Fibroblasts are the most common cell type in connective tissue ECM, in which they synthesize, maintain, and provide a structural framework; fibroblasts secrete the precursor components of the ECM, including the ground substance. Chondrocytes are found in cartilage and produce the cartilagenous matrix. Osteoblasts are responsible for bone formation.
Extracellular matrix in plants
Plant cells are tessellated to form tissues. The cell wall is the relatively rigid structure surrounding the plant cell. The cell wall provides lateral strength to resist osmotic turgor pressure, but it is flexible enough to allow cell growth when needed; it also serves as a medium for intercellular communication. The cell wall comprises multiple laminate layers of cellulose microfibrils embedded in a matrix of glycoproteins, including hemicellulose, pectin, and extensin. The components of the glycoprotein matrix help cell walls of adjacent plant cells to bind to each other. The selective permeability of the cell wall is chiefly governed by pectins in the glycoprotein matrix. Plasmodesmata (singular: plasmodesma) are pores that traverse the cell walls of adjacent plant cells. These channels are tightly regulated and selectively allow molecules of specific sizes to pass between cells.
Extracellular matrix has been found to cause regrowth and healing of tissue. In human fetuses, for example, the extracellular matrix works with stem cells to grow and regrow all parts of the human body, and fetuses can regrow anything that gets damaged in the womb. Scientists have long believed that the matrix stops functioning after full development. It has been used in the past to help horses heal torn ligaments, but it is being researched further as a device for tissue regeneration in humans.
In terms of injury repair and tissue engineering, the extracellular matrix serves two main purposes. First, it prevents the immune system from triggering from the injury and responding with inflammation and scar tissue. Next, it facilitates the surrounding cells to repair the tissue instead of forming scar tissue.
For medical applications, the ECM required is usually extracted from pig bladders, an easily accessible and relatively unused source. It is currently being used regularly to treat ulcers by closing the hole in the tissue that lines the stomach, but further research is currently being done by many universities as well as the U.S. Government for wounded soldier applications. As of early 2007, testing was being carried out on a military base in Texas. Scientists are using a powdered form on Iraq War veterans whose hands were damaged in the war.
Not all ECM devices come from the bladder. Extracellular matrix coming from pig small intestine submucosa are being used to repair "atrial septal defects" (ASD), "patent foramen ovale" (PFO) and inguinal hernia. After one year 95% of the collagen ECM in these patches is replaced by the normal soft tissue of the heart.
Extracellular matrix proteins are commonly used in cell culture systems to maintain stem and precursor cells in an undifferentiated state during cell culture and function to induce differentiation of epithelial, endothelial and smooth muscle cells in vitro. Extracellular matrix proteins can also be used to support 3D cell culture in vitro for modelling tumor development.
A class of biomaterials derived from processing human or animal tissues to retain portions of the extracellular matrix are called ECM Biomaterial.
- Michel, Gurvan; Thierry Tonon; Delphine Scornet; J. Mark Cock; Bernard Kloareg (October 2010). "The cell wall polysaccharide metabolism of the brown alga Ectocarpus siliculosus. Insights into the evolution of extracellular matrix polysaccharides in Eukaryotes". New Phytologist 188 (1): 82–97. doi:10.1111/j.1469-8137.2010.03374.x.
- Abedin, Monika; Nicole King (December 2010). "Diverse evolutionary paths to cell adhesion". Trends in Cell Biology 20 (12): 734–742. doi:10.1016/j.tcb.2010.08.002. PMC 2991404. PMID 20817460.
- Kumar; Abbas; Fausto. Robbins and Cotran: Pathologic Basis of Disease (7th ed.). Philadelphia: Elsevier. ISBN 0-7216-0187-1.
- Alberts B, Bray D, Hopin K, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2004). "Tissues and Cancer". Essential cell biology. New York and London: Garland Science. ISBN 0-8153-3481-8.
- Brownlee, Colin (October 2002). "Role of the extracellular matrix in cell-cell signalling: paracrine paradigms". Current Opinion in Plant Biology 5 (5): 396–401. doi:10.1016/S1369-5266(02)00286-8.
- Kostakioti, Maria (2013). "Bacterial Biofilms: Development, Dispersal, and Therapeutic Strategies in the Dawn of the Postantibiotic Era" (PDF). Cold Spring Harbor Perspectives in Medicine.
- Liotta LA, Tryggvason K, Garbisa S, Hart I, Foltz CM, Shafie S (1980). "Metastatic potential correlates with enzymatic degradation of basement membrane collagen". Nature 284 (5751): 67–68. doi:10.1038/284067a0. PMID 6243750.
- Wang JHC, Thampatty BP, Lin JS, Im HJ (2007). "Mechanoregulation of gene expression in fibroblasts". Gene 15 (391): 1–15. PMID 17331678.
- Engler AJ, Sen S, Sweeney HL, Discher DE (2006). "Matrix elasticity directs stem cell lineage specification". Cell 126 (4): 677–89. PMID 16923388.
- Lo CM, Wang HB, Dembo M, Wang YL (2000). "Cell movement is guided by the rigidity of the substrate". Biophys J 79 (1): 144–52. PMID 10866943.
- Provenzano PP , Inman DR, Eliceiri KW, Keely PJ (2009). "Matrix density-induced mechanoregulation of breast cell phenotype, signaling and gene expression through a FAK-ERK linkage". Oncogene 28 (49): 4326–4343. doi:10.1038/onc.2009.299. PMID 19826415.
- Plopper G (2007). The extracellular matrix and cell adhesion, in Cells (eds Lewin B, Cassimeris L, Lingappa V, Plopper G). Sudbury, MA: Jones and Bartlett. ISBN 0-7637-3905-7.
- Gallagher, J.T., Lyon, M. (2000). "Molecular structure of Heparan Sulfate and interactions with growth factors and morphogens". In Iozzo, M, V. Proteoglycans: structure, biology and molecular interactions. Marcel Dekker Inc. New York, New York. pp. 27–59. ISBN 9780824703349.
- Iozzo, R. V. (1998). "Matrix proteoglycans: from molecular design to cellular function". Annu. Rev. Biochem. 67 (1): 609–652. doi:10.1146/annurev.biochem.67.1.609. PMID 9759499.
- Hensch TK (2005). "Critical period mechanisms in developing visual cortex". Curr. Top. Dev. Biol. 69: 215–237. doi:10.1016/S0070-2153(05)69008-4. PMID 16243601.
- Lodish H, Berk A, Matsudaira P, Kaiser CA, Krieger M, Scott MP, Zipursky SL, Darnell J. "Integrating Cells Into Tissues". Molecular Cell Biology (5th ed.). New York: WH Freeman and Company. pp. 197–234.
- Peach RJ, Hollenbaugh D, Stamenkovic I, Aruffo A (July 1993). "Identification of hyaluronic acid binding sites in the extracellular domain of CD44". J. Cell Biol. 122 (1): 257–264. doi:10.1083/jcb.122.1.257. PMC 2119597. PMID 8314845.
- Di Lullo GA, Sweeney SM, Korkko J, Ala-Kokko L, San Antonio JD (2002). "Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen". J. Biol. Chem. 277 (6): 4223–4231. doi:10.1074/jbc.M110709200. PMID 11704682.
- Karsenty G, Park RW (1995). "Regulation of type I collagen genes expression". Int. Rev. Immunol. 12 (2–4): 177–185. doi:10.3109/08830189509056711. PMID 7650420.
- Kern B, Shen J, Starbuck M, Karsenty G (2001). "Cbfa1 contributes to the osteoblast-specific expression of type I collagen genes". J. Biol. Chem. 276 (10): 7101–7107. doi:10.1074/jbc.M006215200. PMID 11106645.
- Alberts, Bruce (2002). Molecular biology of the cell. Garland Science. ISBN 0-8153-3218-1.
- Plotnikov SV, Pasapera AM, Sabass B, Waterman CM (2012). "Force fluctuations within focal adhesions mediate ECM-rigidity sensing to guide directed cell migration". Cell 151 (7): 1513–27. PMID 23260139.
- Discher DE, Janmey P. and Wang YL (2005). "Tissue cells feel and respond to the stiffness of their substrate". Science 310: 1139–1143. PMID 16293750.
- Hadjipanayi E, Mudera V, Brown RA (2009). "Close dependence of fibroblast proliferation on collagen scaffold matrix stiffness". J. Tissue Eng. 3 (2): 77–84. PMID 19051218.
- Wang HB, Dembo M, Wang YL (2000). "Substrate flexibility regulates growth and apoptosis of normal but not transformed cells". Am J Physiol Cell Physiol 279 (5): 1345–50. PMID 11029281.
- Allen JL, Cooke ME, Alliston T (25 July 2012). "ECM stiffness primes the TGF pathway to promote chondrocyte differentiation". Molecular Biology of the Cell 23 (18): 3731–3742. doi:10.1091/mbc. PMID 22833566.
- Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess HF, Waterman CM (25 November 2010). "Nanoscale architecture of integrin-based cell adhesions". Nature 468 (7323): 580–584. doi:10.1038/nature09621. PMID 21107430.
- 'Pixie dust' helps man grow new finger
- HowStuffWorks, Humans Can Regrow Fingers? In 2009, the St. Francis Heart Center announced the use of the extracellular matrix technology in repair surgery. Archived March 10, 2007 at the Wayback Machine
- "First Ever Implantation of Bioabsorbable Biostar Device at DHZB". DHZB NEWS. December 2007. Retrieved 2008-08-05.
The almost transparent collagen matrix consists of medically purified pig intestine, which is broken down by the scavenger cells (macrophages) of the immune system. After about 1 year the collagen has been almost completely (90-95%) replaced by normal body tissue: only the tiny metal framework remains. An entirely absorbable implant is currently under development.
- Kleinman, H.K.; L. Luckenbill-Edds1, F.W. Cannon, G.C. Sephel (October 1987). "Use of extracellularmatrix components for cell culture". Analytical Biochemistry 186 (1): 1–13. doi:10.1016/0003-2697(87)90538-0. Retrieved 2012-05-14.
- ANAT3231 Lecture 08 Extracellular Matrix[dead link] - Lecture about extracellular matrix from UNSW Cell Biology website.
- Extracellular matrix: review of its roles in acute and chronic wounds
- Usage of Extracellular Matrix from pigs to regrow human extremities
- "The Extracellular Matrix of Animals", from Chapter 19 of The Molecular Biology of the Cell, 4th edition, Alberts et al.
- Biology, John W. Kimball. An online Biology textbook.
- , The man who grew a finger, By Matthew Price, BBC news
- Sound Medicine - Heart Tissue Regeneration[dead link] - July 19 interview discussing ECM and its uses in cardiac tissue repair (requires MP3 playback) [broken link].
- Growing Body Parts - A December 2009 report by 60 Minutes