Jump to content

Granulocyte

From Wikipedia, the free encyclopedia

This is an old revision of this page, as edited by 74.215.81.17 (talk) at 16:30, 18 February 2010. The present address (URL) is a permanent link to this revision, which may differ significantly from the current revision.

Eosinophil granulocyte

Granulocytes are a category of white blood cells characterized by the presence of granules in their cytoplasm.[1] They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes,[2] the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.

Types of granulocytes

There are three types of granulocytes, distinguished by their appearance under Wright's stain:

Their names are Stephen, Mitchell, and Mr Doctoroctagonoman derived from their staining characteristics; for example, the most abundant granulocyte is the neutrophil granulocyte, which has neutrally-staining cytoplasmic granules.

Other white blood cells which are not granulocytes ("agranulocytes") are mainly lymphocytes and monocytes.

Neutrophils

Main article: Neutrophil
A neutrophil with a segmented nucleus (center and surrounded by erythrocytes), the intra-cellular granules are visible in the cytoplasm (Giemsa stained high magnification)

Neutrophils are normally found in the bloodstream and are the most abundant type of phagocyte, constituting 50% to 60% of the total circulating white blood cells.[3] One liter of human blood contains about five billion neutrophils,[4] which are about 10 micrometers in diameter,[5] and live approximately five days.[6] Once neutrophils have received the appropriate signals, it takes them about thirty minutes to leave the blood and reach the site of an infection.[7] Neutrophils are professional phagocytes[8]: they are ferocious eaters and rapidly engulf invaders coated with antibodies and complement, and damaged cells or cellular debris. Neutrophils do not return to the blood; they turn into pus cells and die.[7] Mature neutrophils are smaller than monocytes, and have a segmented nucleus with several sections(two to five segments); each section is connected by chromatin filaments. Neutrophils do not normally exit the bone marrow until maturity but during an infection neutrophil precursors called myelocytes and promyelocytes are released.[9]

The intra-cellular granules of the human neutrophil have long been recognized for their protein-destroying and bactericidal properties.[10] Neutrophils can secrete products that stimulate monocytes and macrophages; these secretions increase phagocytosis and the formation of reactive oxygen compounds involved in intracellular killing.[11] Neutrophils have two types of granules primary (azurophilic) granules (found in young cells) and specific granules (which are found in more mature cells). Primary granules contain cationic proteins and defensins that are used to kill bacteria, proteolytic enzymes and cathepsin G to breakdown (bacterial) proteins, lysozyme to break down bacterial cell walls, and myeloperoxidase (use to generate toxic bacteria-killing substances).[12] In addition, secretions from the primary granules of neutrophils stimulate the phagocytosis of IgG antibody-coated bacteria.[13] The secondary granules contain compounds that are involved in the formation of toxic oxygen compounds, lysozyme, and lactoferrin (used to take essential iron from bacteria).[12]

Eosinophils

Main article: Eosinophil
An eosinophil surrounded by erythrocytes

Eosinophils also have lobed nuclei (two to four lobes). The number of granules in an eosinophil can vary because they have a tendency to degranulate while in the blood stream.[14] Eosinophils play a crucial part in the killing of parasites (e.g. enteric nematodes) because their granules contain a unique, toxic basic protein and cationic protein (e.g. cathepsin[12]);[15] receptors that bind to IgE are used to help with this task.[16] These cells also have a limited ability to participate in phagocytosis,[17] they are professional antigen presenting cells, they regulate other immune cell functions (e.g. CD4+ T cell, dendritic cell, B cell, mast cell, neutrophil, and basophil functions),[18] they are involved in the destruction of tumor cells[14], and they promote the repair of damaged tissue.[19] A chemical called interleukin-5 interacts with eosinophils and causes them to grow and differentiate; this chemical is produced by basophils. [15]

Basophils

Main article: Basophil
A basophil with lobed nuclei surrounded by erythrocytes

Basophils are one of the least abundant cells in bone marrow and blood (occurring at less than two percent of all cells). Like neutrophils and eosinophils they have lobed nuclei; however they only have two lobes and the chromatin filaments that connect them are not very visible. Basophils have receptors that can bind to IgE, IgG, complement, and histamine. The cytoplasm of basophils contains a varied amount of granules; these granules are usually numerous enough to partially conceal the nucleus. Granule contents of basophils are abundant with histamine, heparin, chondroitin sulfate, peroxidase, platelet activating factor, and other substances.

When an infection occurs mature basophils will be released from the bone marrow and travel to the site of infection.[20] When basophils are injured they will release histamine; this contributes to the inflammatory response that helps fight invading organisms. Histamine causes dilation and increased permeability of capillaries close to the basophil. Injured basophils and other leukocytes will release another substance called prostaglandins that contributes to an increased blood flow to the site of infection. Both of these mechanisms allow blood clotting elements to be delivered to the infected area (this begins the recovery process and blocks the travel of microbes to other parts of the body. Increased permeability of the blood also allows for more phagocyte migration to the site of infection so that they can consume microbes.[17]

Toxic materials produced or released

Examples of toxic materials produced or released by degranulation by granulocytes on the ingestion of microorganism includes:

Pathology

Granulocytopenia is an abnormally low concentration of granulocytes in the blood. This condition reduces the body's resistance to many infections. Closely-related terms include agranulocytosis (no granulocytes at all) and neutropenia (deficiency of neutrophil granulocytes). Transfusion of granulocytes would have been a solution to the problem. However, granulocytes live only ~10 hours in the circulation (for days in spleen or other tissue), which gives a very short-lasting effect. In addition, there are many complications of such procedure.

A granuloma is a tumor containing granulocytes, and a "granulomatosis" is a necrotizing granuloma.

There is usually a granulocyte chemotactic defect in individuals who suffer from insulin dependent diabetes mellitus.

Additional images

  • Blood cell lineage
    Blood cell lineage
  • Hematopoiesis
    Hematopoiesis

See also

References

  1. ^ Template:EMedicineDictionary
  2. ^ Template:EMedicineDictionary
  3. ^ Stvrtinová, Viera (1995). "Neutrophils, central cells in acute inflammation". Inflammation and Fever from Pathophysiology: Principles of Disease. Computing Centre, Slovak Academy of Sciences: Academic Electronic Press. ISBN 80-967366-1-2. Retrieved March 28, 2009. {{cite book}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  4. ^ Hoffbrand p. 331
  5. ^ Delves p. 4
  6. ^ Sompayrac p. 17
  7. ^ a b Sompayrac p. 18
  8. ^ Robinson p. 187 and Ernst pp. 7–10
  9. ^ Linderkamp O, Ruef P, Brenner B, Gulbins E, Lang F (1998). "Passive deformability of mature, immature, and active neutrophils in healthy and septicemic neonates". Pediatr. Res. 44 (6): 946–50. doi:10.1203/00006450-199812000-00021. PMID 9853933. Retrieved 2009-04-06. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  10. ^ Paoletti p. 62
  11. ^ Soehnlein O, Kenne E, Rotzius P, Eriksson EE, Lindbom L (2008). "Neutrophil secretion products regulate anti-bacterial activity in monocytes and macrophages". Clin. Exp. Immunol. 151 (1): 139–45. doi:10.1111/j.1365-2249.2007.03532.x. PMC 2276935. PMID 17991288. Retrieved March 29, 2009. {{cite journal}}: Unknown parameter |doi_brokendate= ignored (|doi-broken-date= suggested) (help); Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  12. ^ a b c Mayer, Gene (2006). "Immunology — Chapter One: Innate (non-specific) Immunity". Microbiology and Immunology On-Line Textbook. USC School of Medicine. Retrieved November 12, 2008.
  13. ^ Soehnlein O, Kai-Larsen Y, Frithiof R; et al. (2008). "Neutrophil primary granule proteins HBP and HNP1-3 boost bacterial phagocytosis by human and murine macrophages". J. Clin. Invest. 118 (10): 3491–502. doi:10.1172/JCI35740. PMC 2532980. PMID 18787642. {{cite journal}}: |access-date= requires |url= (help); Explicit use of et al. in: |author= (help); Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  14. ^ a b Hess, Charles E. "Segmented Eosinophil". University of Virginia Health System. Retrieved 2009-04-10.
  15. ^ a b Baron, Samuel (editor) (1996). Medical Microbiology (4th edition). EditionThe University of Texas Medical Branch at Galveston. ISBN ISBN 0-9631172-1-1. {{cite book}}: |first= has generic name (help); Check |isbn= value: invalid character (help); Cite has empty unknown parameter: |coauthors= (help)
  16. ^ Gleich GJ, Adolphson CR (1986). "The eosinophilic leukocyte: structure and function". Advances in Immunology. 39: 177–253. doi:10.1016/S0065-2776(08)60351-X. PMID 3538819.
  17. ^ a b Campbell p. 903
  18. ^ Akuthota P, Wang HB, Spencer LA, Weller PF (2008). "Immunoregulatory roles of eosinophils: a new look at a familiar cell". Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology. 38 (8): 1254–63. doi:10.1111/j.1365-2222.2008.03037.x. PMC 2735457. PMID 18727793. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  19. ^ Kariyawasam HH, Robinson DS (2006). "The eosinophil: the cell and its weapons, the cytokines, its locations". Seminars in Respiratory and Critical Care Medicine. 27 (2): 117–27. doi:10.1055/s-2006-939514. PMID 16612762. {{cite journal}}: Unknown parameter |month= ignored (help)
  20. ^ Hess, Charles E. "Mature Basophil". University of Virginia Health System. Retrieved 2009-04-10.
Bibliography
  • Campbell, Neil A., Reece Jane B., Biology (6th edition), Pearson Education, Inc., 2002 ISBN 0-8053-6624-5
  • Delves, P.J., Martin, S. J., Burton, D. R. and Roit I.M. Roitt's Essential Immunology (11th edition), Blackwell Publishing, 2006, ISBN 978-1-4051-3603-7.
  • Ernst J. D. and Stendahl O., (editors), Phagocytosis of Bacteria and Bacterial Pathogenicity, Cambridge University Press, 2006, ISBN 0-521-84569-6 Website
  • Hoffbrand, A.V., Pettit, J.E. and Moss, P.A.H., Essential Haematology (4th edition), Blackwell Science, 2005, ISBN 0-632-05153-1.
  • Paoletti R., Notario A. and Ricevuti G., (editors), Phagocytes: Biology, Physiology, Pathology, and Pharmacotherapeutics, The New York Academy of Sciences, 1997, ISBN 1-57331-102-2.
  • Robinson J.P. and Babcock G. F., (editors), Phagocyte Function —A guide for research and clinical evaluation, Wiley–Liss, 1998, ISBN 0471123641
  • Sompayrac, L. How the Immune System Works (3rd edition), Blackwell Publishing, 2008, ISBN 978-1-4051-6221-0
Retrieved from "https://en.wikipedia.org/w/index.php?title=Granulocyte&oldid=344838947"