Gut–brain axis
![](http://upload.wikimedia.org/wikipedia/commons/thumb/3/39/GI_normal.jpg/220px-GI_normal.jpg)
The gut–brain axis is the biochemical signaling that takes place between the gastrointestinal tract and the central nervous system.[1] That term has been expanded to include the role of the gut flora in the interplay; the term microbiome-gut-brain axis is sometimes used to describe paradigms explicitly including the gut flora.[1][2][3]
Broadly defined, the gut-brain axis includes the central nervous system, neuroendocrine and neuroimmune systems including the hypothalamic–pituitary–adrenal axis (HPA axis), sympathetic and parasympathetic arms of the autonomic nervous system including the enteric nervous system and the vagus nerve, and the gut microbiota.[1][3]
Interest in the field was sparked by a 2004 study showing that germ-free mice showed an exaggerated HPA axis response to stress compared to non-GF laboratory mice.[1] As of January 2016, most of the work that has been done on the role of gut flora in the gut-brain axis had been conducted in animals, or characterizing the various neuroactive compounds that gut flora can produce, and studies with humans measuring differences between people with various psychiatric and neurological differences, or changes to gut flora in response to stress, or measuring effects of various probiotics (dubbed "psychobiotics" in this context), had generally been small and could not be generalized; whether changes to gut flora are a result of disease, a cause of disease, or both in any number of possible feedback loops in the gut-brain axis, remained unclear.[4][1] A systematic review from 2016 examined the preclinical and small human trials that have been conducted with certain commercially available strains of probiotic bacteria and identified those that had the most potential to be useful for certain central nervous system disorders.[5]
![](http://upload.wikimedia.org/wikipedia/commons/thumb/3/35/Bifidobacterium_adolescentis_Gram.jpg/220px-Bifidobacterium_adolescentis_Gram.jpg)
![](http://upload.wikimedia.org/wikipedia/commons/thumb/7/7d/Lactobacillus_sp_01.png/220px-Lactobacillus_sp_01.png)
Gut flora
The gut flora is the complex community of microorganisms that live in the digestive tracts of humans and other animals, as well as insects. The gut metagenome is the aggregate of all the genomes of gut microbiota.[6] The gut is one niche that human microbiota inhabit.[7]
In humans, the gut microbiota has the largest numbers of bacteria and the greatest number of species compared to other areas of the body.[8] In humans the gut flora is established at one to two years after birth, and by that time the intestinal epithelium and the intestinal mucosal barrier that it secretes have co-developed in a way that is tolerant to, and even supportive of, the gut flora and that also provides a barrier to pathogenic organisms.[9][10]
The relationship between gut flora and humans is not merely commensal (a non-harmful coexistence), but rather a mutualistic relationship.[7]: 700 Human gut microorganisms benefit the host by collecting the energy from the fermentation of undigested carbohydrates and the subsequent absorption of short-chain fatty acids (SCFAs), acetate, butyrate, and propionate.[8][11] Intestinal bacteria also play a role in synthesizing vitamin B and vitamin K as well as metabolizing bile acids, sterols, and xenobiotics.[7][11] The systemic importance of the SCFAs and other compounds they produce are like hormones and the gut flora itself appears to function like an endocrine organ,[11] and dysregulation of the gut flora has been correlated with a host of inflammatory and autoimmune conditions.[8][12]
The composition of human gut flora changes over time, when the diet changes, and as overall health changes.[8][12]
Enteric nervous system
The enteric nervous system is one of the main divisions of the nervous system and consists of a mesh-like system of neurons that governs the function of the gastrointestinal system; it has been described as a "second brain" for several reasons. The enteric nervous system can operate autonomously. It normally communicates with the central nervous system (CNS) through the parasympathetic (e.g., via the vagus nerve) and sympathetic (e.g., via the prevertebral ganglia) nervous systems. However, vertebrate studies show that when the vagus nerve is severed, the enteric nervous system continues to function.[13]
In vertebrates, the enteric nervous system includes efferent neurons, afferent neurons, and interneurons, all of which make the enteric nervous system capable of carrying reflexes in the absence of CNS input. The sensory neurons report on mechanical and chemical conditions. Through intestinal muscles, the motor neurons control peristalsis and churning of intestinal contents. Other neurons control the secretion of enzymes. The enteric nervous system also makes use of more than 30 neurotransmitters, most of which are identical to the ones found in CNS, such as acetylcholine, dopamine, and serotonin. More than 90% of the body's serotonin lies in the gut, as well as about 50% of the body's dopamine and the dual function of these neurotransmitters is an active part of gut-brain research.[14][15][16]
Gut-brain integration
![](http://upload.wikimedia.org/wikipedia/commons/thumb/b/bc/MorrisWaterMaze.jpg/220px-MorrisWaterMaze.jpg)
The gut–brain axis, a bidirectional neurohumoral communication system, is important for maintaining homeostasis and is regulated through the central and enteric nervous systems and the neural, endocrine, immune, and metabolic pathways, and especially including the hypothalamic–pituitary–adrenal axis (HPA axis).[1] That term has been expanded to include the role of the gut flora as part of the "microbiome-gut-brain axis", a linkage of functions including the gut flora.[1][3][2]
Interest in the field was sparked by a 2004 study showing that germ-free mice (genetically homogeneous laboratory mice, birthed and raised in an antiseptic environment) showed an exaggerated HPA axis response to stress compared to non-GF laboratory mice.[1]
The gut flora can produce a range of neuroactive molecules, such as acetylcholine, catecholamines, γ-aminobutyric acid, histamine, melatonin, and serotonin, which is essential for regulating peristalsis and sensation in the gut.[17] Changes in the composition of the gut flora due to diet, drugs, or disease correlate with changes in levels of circulating cytokines, some of which can affect brain function.[17] The gut flora also release molecules that can directly activate the vagus nerve which transmits information about the state of the intestines to the brain.[17]
Likewise, chronic or acutely stressful situations activate the hypothalamic–pituitary–adrenal axis, causing changes in the gut flora and intestinal epithelium, and possibly having systemic effects.[17] Additionally, the cholinergic anti-inflammatory pathway, signaling through the vagus nerve, affects the gut epithelium and flora.[17] Hunger and satiety are integrated in the brain, and the presence or absence of food in the gut and types of food present, also affect the composition and activity of gut flora.[17]
That said, most of the work that has been done on the role of gut flora in the gut-brain axis has been conducted in animals, including the highly artificial germ-free mice. As of 2016 studies with humans measuring changes to gut flora in response to stress, or measuring effects of various probiotics, have generally been small and cannot be generalized; whether changes to gut flora are a result of disease, a cause of disease, or both in any number of possible feedback loops in the gut-brain axis, remains unclear.[4]
Specific conditions
Anxiety and mood disorders
As of January 2016 work on the relationship between gut flora and anxiety disorders and mood disorders including depression was at an early stage, with insufficient evidence to draw conclusions about a causal role for gut flora changes in these conditions, nor for the efficacy of any probiotic treatment.[4]
![](http://upload.wikimedia.org/wikipedia/commons/thumb/2/2e/Lab_mouse_mg_3135.jpg/220px-Lab_mouse_mg_3135.jpg)
People with anxiety and mood disorders tend to have GI problems; small studies have been conducted to compare the gut flora of people with major depressive disorder and healthy people, but those studies have had contradictory results.[4]
Much interest was generated in the potential role of gut flora in anxiety disorders, and more generally in the role of gut flora in the gut-brain axis, by studies published in 2004 showing that germ-free mice have an exaggerated HPA axis response to stress caused by being restrained, which was reversed by colonizing their gut with a Bifidobacterium species.[2] Studies looking at maternal separation for rats shows neonatal stress leads to long-term changes in the gut microbiota such as its diversity and composition, which also led to stress and anxiety-like behavior.[18] Additionally, while much work had been done as of 2016 to characterize various neurotransmitters known to be involved in anxiety and mood disorders that gut flora can produce (for example, Escherichia, Bacillus, and Saccharomyces species can produce noradrenalin; Candida, Streptococcus, and Escherichia species can produce serotonin, etc) the inter-relationships and pathways by which the gut flora might affect anxiety in humans were unclear.[6]
Schizophrenia
People with schizophrenia tend to also have GI problems,[17] but as of 2015, no studies had been carried out to compare the gut flora of people with schizophrenia with healthy people.[3] Research causing schizophrenia-like symptoms in mice by giving them phencyclidine (PCP) has found changes to the gut flora of the treated mice compared with untreated mice.[3]
Autism
Around 70% of people with autism also have GI problems, and autism is often diagnosed at the time that the gut flora becomes established, indicating that there may be a connection between autism and gut flora.[19] Some studies have found differences in the gut flora of children with autism compared with normal children – most notably elevations in the amount of Clostridium in the stools of children with autism compared with the stools of the children without[20] – but these results have not been consistently replicated.[19] Many of the environmental factors thought to be relevant to the development of autism would also affect the gut flora, leaving open the question whether specific developments in the gut flora drive the development of autism or whether those developments happen concurrently.[3][19] As of 2016, studies with probiotics had only been conducted with animals; studies of other dietary changes to treat autism have been inconclusive.[4]
Other factors that may be involved in the microbiota-gut-brain axis and have been speculated to be involved in the etiology or pathophysiology of autism is the lack of macrobiota species, namely helminths and fauna, which co-evolved with the microbiota to promote a balanced immune response[21]
Parkinson's disease
As of 2015 one study had been conducted comparing the gut flora of people with Parkinson's disease to healthy controls; in that study people with Parkinsons had lower levels of Prevotellaceae and people with Parkinsons who had higher levels of Enterobacteriaceae had more clinically severe symptoms; the authors of the study drew no conclusions about whether gut flora changes were driving the disease or vice versa.[3]
Obsessive–compulsive disorder
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Psychobiotics
Psychobiotics are defined by Dinan et al. as probiotics that may benefit people with psychiatric, or neurological illnesses.[22] The concept generated enormous research interest.[23][24][25][26]
A systematic review from 2016 examined the preclinical and small human trials that have been conducted with certain commercially available strains of probiotic bacteria and found that among those tested, Bifidobacterium and Lactobacillus genera (B. longum, B. breve, B. infantis, L. helveticus, L. rhamnosus, L. plantarum, and L. casei), had the most potential to be useful for certain central nervous system disorders.[5]
References
- ^ a b c d e f g h Wang, Y; Kasper, LH (May 2014). "The role of microbiome in central nervous system disorders". Brain Behav Immun. 38: 1–12. doi:10.1016/j.bbi.2013.12.015. PMC 4062078. PMID 24370461.
- ^ a b c Mayer, EA; Knight, R; Mazmanian, SK; et al. (2014). "Gut microbes and the brain: paradigm shift in neuroscience" (PDF). J Neurosci. 34: 15490–15496. doi:10.1523/JNEUROSCI.3299-14.2014. PMC 4228144. PMID 25392516.
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(help) - ^ a b c d e f g Dinan, T.G; Cryan, 2015 (2015). "The impact of gut microbiota on brain and behavior: implications for psychiatry". Curr Opin Clin Nutr Metab Care. 18: 552–558. doi:10.1097/MCO.0000000000000221. PMID 26372511.
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has numeric name (help) - ^ a b c d e Schneiderhan, J; Master-Hunter, T; Locke, A (2016). "Targeting gut flora to treat and prevent disease". J Fam Pract. 65: 34–8. PMID 26845162.
- ^ a b Wang H, Lee IS, Braun C, Enck P (July 2016). "Effect of probiotics on central nervous system functions in animals and humans - a systematic review". J. Neurogastroenterol Motil. doi:10.5056/jnm16018. PMID 27413138.
We reviewed the effect of probiotics on the central nervous system in randomized controlled trials in animals and humans, and analyzed the possibility of translating animal models to human studies because few human studies have been conducted to date. According to the qualitative analyses of current studies, we can provisionally draw the conclusion that B. longum, B. breve, B. infantis, L. helveticus, L. rhamnosus, L. plantarum, and L. casei were most effective in improving CNS function, including psychiatric disease-associated functions (anxiety, depression, mood, stress response) and memory abilities.
- ^ a b Saxena, R.; Sharma, V.K (2016). "A Metagenomic Insight Into the Human Microbiome: Its Implications in Health and Disease". In D. Kumar; S. Antonarakis (eds.). Medical and Health Genomics. Elsevier Science. p. 117. doi:10.1016/B978-0-12-420196-5.00009-5. ISBN 978-0-12-799922-7.
- ^ a b c Sherwood, Linda; Willey, Joanne; Woolverton, Christopher (2013). Prescott's Microbiology (9th ed.). New York: McGraw Hill. pp. 713–721. ISBN 978-0-07-340240-6. OCLC 886600661.
- ^ a b c d Quigley, EM (2013). "Gut bacteria in health and disease". Gastroenterol Hepatol (N Y). 9: 560–9. PMC 3983973. PMID 24729765.
- ^ Sommer, F; Bäckhed, F (Apr 2013). "The gut microbiota--masters of host development and physiology". Nat Rev Microbiol. 11 (4): 227–38. doi:10.1038/nrmicro2974. PMID 23435359.
- ^ Faderl, M; et al. (Apr 2015). "Keeping bugs in check: The mucus layer as a critical component in maintaining intestinal homeostasis". IUBMB Life. 67 (4): 275–85. doi:10.1002/iub.1374. PMID 25914114.
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(help) - ^ a b c Clarke, G; et al. (Aug 2014). "Minireview: Gut microbiota: the neglected endocrine organ". Mol Endocrinol. 28 (8): 1221–38. doi:10.1210/me.2014-1108. PMID 24892638.
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(help) - ^ a b Shen, S; Wong, CH (Apr 2016). "Bugging inflammation: role of the gut microbiota". Clin Transl Immunology. 5 (4): e72. doi:10.1038/cti.2016.12. PMC 4855262. PMID 27195115.
- ^ Li,Ying; Owyang,Chung (September 2003). "Musings on the Wanderer: What's New in Our Understanding of Vago-Vagal Reflexes? V. Remodeling of vagus and enteric neural circuitry after vagal injury". American Journal of Physiology. Gastrointestinal and Liver Physiology. 285 (3): G461-9. doi:10.1152/ajpgi.00119.2003.
- ^ Pasricha, Pankaj Jay. "Stanford Hospital: Brain in the Gut – Your Health".
- ^ Martinucci, I; et al. (2015). "Genetics and pharmacogenetics of aminergic transmitter pathways in functional gastrointestinal disorders". Pharmacogenomics. 16 (5): 523–39. doi:10.2217/pgs.15.12. PMID 25916523.
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(help) - ^ Smitka, K; et al. (2013). "The role of "mixed" orexigenic and anorexigenic signals and autoantibodies reacting with appetite-regulating neuropeptides and peptides of the adipose tissue-gut-brain axis: relevance to food intake and nutritional status in patients with anorexia nervosa and bulimia nervosa". Int J Endocrinol. 2013: 483145. doi:10.1155/2013/483145. PMC 3782835. PMID 24106499.
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(help)CS1 maint: unflagged free DOI (link) - ^ a b c d e f g Petra, AI; et al. (May 2015). "Gut-Microbiota-Brain Axis and Its Effect on Neuropsychiatric Disorders With Suspected Immune Dysregulation". Clin Ther. 37 (5): 984–95. doi:10.1016/j.clinthera.2015.04.002. PMC 4458706. PMID 26046241.
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(help) - ^ Foster, J.A.; McVey Neufelt, K.A. (2013). "Gut-brain axis: how the microbiome influences anxiety and depression". Trends in Neurosciences. 36: 305–312. doi:10.1016/j.tins.2013.01.005. PMID 23384445.
- ^ a b c Buie, T (May 2015). "Potential Etiologic Factors of Microbiome Disruption in Autism". Clin Ther. 37 (5): 976–83. doi:10.1016/j.clinthera.2015.04.001. PMID 26046240.
- ^ Chen, X; D'Souza, R; Hong, ST (2013). "The role of gut microbiota in the gut-brain axis: current challenges and perspectives". Protein & Cell. 4 (6): 403–14. doi:10.1007/s13238-013-3017-x. PMID 23686721.
- ^ Slattery, John; MacFabe, Derrick F.; Kahler, Stephen G.; Frye, Richard E. (2016-01-01). "Enteric Ecosystem Disruption in Autism Spectrum Disorder: Can the Microbiota and Macrobiota be Restored?". Current Pharmaceutical Design. 22 (40): 6107–6121. ISSN 1873-4286. PMID 27592717.
- ^ Dinan TG, Stanton C, Cryan JF (2013). "Psychobiotics: a novel class of psychotropic". Biol Psychiatry. 74 (10): 720–6. doi:10.1016/j.biopsych.2013.05.001. PMID 23759244.
- ^ Zhou L, Foster JA (2015). "Psychobiotics and the gut-brain axis: in the pursuit of happiness". Neuropsychiatr Dis Treat. 11: 715–23. doi:10.2147/NDT.S61997. PMC 4370913. PMID 25834446.
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: CS1 maint: unflagged free DOI (link) - ^ Gnanavel S (2015). "Psychobiotics: the latest psychotropics". Indian J Psychol Med. 37 (1): 110. doi:10.4103/0253-7176.150862. PMC 4341301. PMID 25722529.
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: CS1 maint: unflagged free DOI (link) - ^ Evrensel A, Ceylan ME (2015). "The Gut-Brain Axis: The Missing Link in Depression". Clin Psychopharmacol Neurosci. 13 (3): 239–244. doi:10.9758/cpn.2015.13.3.239. PMC 4662178. PMID 26598580.
- ^ Selhub EM, Logan AC, Bested AC (2014). "Fermented foods, microbiota, and mental health: ancient practice meets nutritional psychiatry". J Physiol Anthropol. 33: 2. doi:10.1186/1880-6805-33-2. PMC 3904694. PMID 24422720.
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: CS1 maint: unflagged free DOI (link)
Further reading
- Hooper LV; Macpherson AJ (Mar 2010). "Immune adaptations that maintain homeostasis with the intestinal microbiota". Nat Rev Immunol. 10: 159–69. doi:10.1038/nri2710. PMID 20182457..
- De Palma, G; Collins, SM; Bercik, P (2014). "The microbiota-gut-brain axis in functional gastrointestinal disorders". Gut Microbes. 5: 419–29. doi:10.4161/gmic.29417. PMC 4153782. PMID 24921926. Free PMC Article