5β-Reductase
| Delta4-3-oxosteroid 5beta-reductase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.3.1.3 | ||||||||
| CAS no. | 2604034 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Delta4-3-oxosteroid 5beta-reductase (EC 1.3.1.3, 3-oxo-Delta4-steroid 5beta-reductase, 5beta-reductase, androstenedione 5beta-reductase, cholestenone 5beta-reductase, cortisone 5beta-reductase, cortisone beta-reductase, cortisone Delta4-5beta-reductase, steroid 5beta-reductase, testosterone 5beta-reductase, Delta4-3-ketosteroid 5beta-reductase, Delta4-5beta-reductase, Delta4-hydrogenase, 4,5beta-dihydrocortisone:NADP+ Delta4-oxidoreductase, 3-oxo-5beta-steroid:NADP+ Delta4-oxidoreductase) is an enzyme with system name 5beta-cholestan-3-one:NADP+ 4,5-oxidoreductase.[1][2][3][4][5][6][7][8][9] This enzyme catalyses the following chemical reaction
- (1) 5beta-cholestan-3-one + NADP+ cholest-4-en-3-one + NADPH + H+
- (2) 17,21-dihydroxy-5beta-pregnane-3,11,20-trione + NADP+ cortisone + NADPH + H+
The human enzyme efficiently acts on progesterone, androstenedione, 17alpha-hydroxyprogesterone and testosterone to 5beta-reduced metabolites. It can also act on aldosterone, corticosterone and cortisol, to some extent.
References
- ^ Forchielli, E. and Dorfman, R.I. (1956). "Separation of Δ4-5α- and Δ4-5β-hydrogenases from rat liver homogenates". J. Biol. Chem. 223: 443–448. PMID 13376613.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Brown-Grant, K., Forchielli, E. and Dorfman, R.I. (1960). "The Δ4-hydrogenases of guinea pig adrenal gland". J. Biol. Chem. 235: 1317–1320. PMID 13805063.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Levy, H.R. and Talalay, P. (1957). "Enzymatic introduction of double bonds into steroid ring A". J. Am. Chem. Soc. 79: 2658–2659. doi:10.1021/ja01567a089.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Tomkins, G.M. (1957). "The enzymatic reduction of Δ4-3-ketosteroids". J. Biol. Chem. 225: 13–24. PMID 13416214.
- ^ Sugimoto, Y., Yoshida, M. and Tamaoki, B. (1990). "Purification of 5β-reductase from hepatic cytosol fraction of chicken". J. Steroid Biochem. 37: 717–724. doi:10.1016/0960-0760(90)90356-p. PMID 2278855.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Furuebisu, M., Deguchi, S. and Okuda, K. (1987). "Identification of cortisone 5β-reductase as Δ4-3-ketosteroid 5β-reductase". Biochim. Biophys. Acta. 912: 110–114. doi:10.1016/0167-4838(87)90253-6. PMID 3828348.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Okuda, A. and Okuda, K. (1984). "Purification and characterization of Δ4-3-ketosteroid 5β-reductase". J. Biol. Chem. 259: 7519–7524. PMID 6736016.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Charbonneau, A. and The, V.L. (2001). "Genomic organization of a human 5β-reductase and its pseudogene and substrate selectivity of the expressed enzyme". Biochim. Biophys. Acta. 1517: 228–235. doi:10.1016/s0167-4781(00)00278-5. PMID 11342103.
{{cite journal}}: CS1 maint: multiple names: authors list (link) - ^ Kondo, K.H., Kai, M.H., Setoguchi, Y., Eggertsen, G., Sjöblom, P., Setoguchi, T., Okuda, K.I. and Björkhem, I. (1994). "Cloning and expression of cDNA of human Δ4-3-oxosteroid 5β-reductase and substrate specificity of the expressed enzyme". Eur. J. Biochem. 219: 357–363. doi:10.1111/j.1432-1033.1994.tb19947.x. PMID 7508385.
{{cite journal}}: CS1 maint: multiple names: authors list (link)
External links
- Delta4-3-oxosteroid+5beta-reductase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)