Bordet–Gengou agar
Bordet-Gengou agar is a type of agar plate optimized to isolate Bordetella, containing blood, potato extract, and glycerol, with an antibiotic such as cephalexin or penicillin and sometimes nicotinamide.[1] The potato extract provided nitrogen and vitamins, and potato starch absorbed fatty acids present in nasal secretions or collection-swab cotton that inhibited growth; glycerol was a carbon source.[2] Medical Microbiology, 4th edition[1] states that Regan-Lowe medium[2] (containing charcoal, blood, and antibiotic) has replaced Bordet-Gengou medium as the medium of choice for routine Bordetella pertussis incubation.
Bordetella bacteria were difficult to culture; Jules Bordet and Octave Gengou invented the first version to isolate the coccobacillus, named Bordet-Gengou in 1906, they believed was associated with whooping cough. In 1909, it was not yet established that the coccobacillus was solely the cause.[3]
References
- ^ a b Horst Finger & Carl Heinz Wirsing von Koenig (1996). "Bordetella". In Samuel Baron (ed.). Medical Microbiology (4th ed.). University of Texas Medical Branch at Galveston. ISBN 0-9631172-1-1.
- ^ a b BD Bordet Gengou Agar with 15% Sheep Blood (product insert also describing Bordetella Agar with Charcoal)
- ^ Martha Wollstein (January 9, 1909). "THE BORDET-GENGOU BACILLUS OF PERTUSSIS". The Journal of Experimental Medicine. 11: 41. doi:10.1084/jem.11.1.41. PMC 2124697. PMID 19867243.
- Kurzynski TA, Boehm DM, Rott-Petri JA, Schell RF, Allison PE (December 1988). "Comparison of modified Bordet-Gengou and modified Regan-Lowe media for the isolation of Bordetella pertussis and Bordetella parapertussis". J. Clin. Microbiol. 26 (12): 2661–3. PMC 266968. PMID 2906642.