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REV1 Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2EBW , 2LSI , 2LSK , 2LSY , 3GQC , 3VU7 , 4BA9 , 4EXT , 4GK0 , 4GK5 , 2N1G
Identifiers Aliases REV1 , REV1L, AIBP80, DNA directed polymerase, REV1 DNA directed polymeraseExternal IDs OMIM : 606134 ; MGI : 1929074 ; HomoloGene : 32309 ; GeneCards : REV1 ; OMA :REV1 - orthologs Wikidata
DNA repair protein REV1 is a protein that in humans is encoded by the REV1 gene .[ 5] [ 6]
This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain , which is important in protein-protein interactions . A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found.[ 6]
Rev1 is a Y family DNA polymerase ; it is sometimes referred to as a deoxycytidyl transferase because it only inserts deoxycytidine (dC) across from lesions. Whether G , A , T , C , or an abasic site , Rev1 will always add a C. Rev1 has the ability to always add a C, because it uses an arginine as a template which complements well with C.[ 7] Yet it is believed[by whom? ] that Rev1 rarely uses its polymerase activity; rather it is thought that Rev1's primary role is as a protein landing pad, whereby it helps direct the recruitment of TLS proteins, especially Pol ζ (Rev3 /Rev7).
Interactions
REV1 has been shown to interact with MAD2L2 .[ 8] It is believed that Rev1 may interact with PCNA, once ubiquitylated due to a lesion, and help recruit Pol ζ (Rev3 /Rev7) a B family polymerase involved in TLS.
References
^ a b c GRCh38: Ensembl release 89: ENSG00000135945 – Ensembl , May 2017
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000026082 – Ensembl , May 2017
^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ Lin W, Xin H, Zhang Y, Wu X, Yuan F, Wang Z (Dec 1999). "The human REV1 gene codes for a DNA template-dependent dCMP transferase" . Nucleic Acids Res . 27 (22): 4468–75. doi :10.1093/nar/27.22.4468 . PMC 148731 . PMID 10536157 .
^ a b "Entrez Gene: REV1 REV1 homolog (S. cerevisiae)" .
^ Nair, DT (Sep 30, 2005). "Rev1 employs a novel mechanism of DNA synthesis using a protein template". Science . 309 (5744): 2219–22. Bibcode :2005Sci...309.2219N . doi :10.1126/science.1116336 . PMID 16195463 . S2CID 35378034 .
^ Murakumo, Y; Ogura Y; Ishii H; Numata S; Ichihara M; Croce C M; Fishel R; Takahashi M (September 2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7" . J. Biol. Chem . 276 (38). United States: 35644–51. doi :10.1074/jbc.M102051200 . ISSN 0021-9258 . PMID 11485998 .
Further reading
Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery" . Genome Res . 6 (9): 791–806. doi :10.1101/gr.6.9.791 . PMID 8889548 .
Wixler V, Laplantine E, Geerts D, et al. (1999). "Identification of novel interaction partners for the conserved membrane proximal region of alpha-integrin cytoplasmic domains" . FEBS Lett . 445 (2–3): 351–5. doi :10.1016/S0014-5793(99)00151-9 . PMID 10094488 . S2CID 9218762 .
Gibbs PE, Wang XD, Li Z, et al. (2000). "The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light" . Proc. Natl. Acad. Sci. U.S.A . 97 (8): 4186–91. Bibcode :2000PNAS...97.4186G . doi :10.1073/pnas.97.8.4186 . PMC 18191 . PMID 10760286 .
Wixler V, Geerts D, Laplantine E, et al. (2000). "The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes" . J. Biol. Chem . 275 (43): 33669–78. doi :10.1074/jbc.M002519200 . PMID 10906324 .
Masuda Y, Takahashi M, Tsunekuni N, et al. (2001). "Deoxycytidyl transferase activity of the human REV1 protein is closely associated with the conserved polymerase domain" . J. Biol. Chem . 276 (18): 15051–8. doi :10.1074/jbc.M008082200 . PMID 11278384 .
Murakumo Y, Ogura Y, Ishii H, et al. (2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7" . J. Biol. Chem . 276 (38): 35644–51. doi :10.1074/jbc.M102051200 . PMID 11485998 .
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences" . Proc. Natl. Acad. Sci. U.S.A . 99 (26): 16899–903. Bibcode :2002PNAS...9916899M . doi :10.1073/pnas.242603899 . PMC 139241 . PMID 12477932 .
Masuda Y, Ohmae M, Masuda K, Kamiya K (2003). "Structure and enzymatic properties of a stable complex of the human REV1 and REV7 proteins" . J. Biol. Chem . 278 (14): 12356–60. doi :10.1074/jbc.M211765200 . PMID 12529368 .
Clark DR, Zacharias W, Panaitescu L, McGregor WG (2004). "Ribozyme-mediated REV1 inhibition reduces the frequency of UV-induced mutations in the human HPRT gene" . Nucleic Acids Res . 31 (17): 4981–8. doi :10.1093/nar/gkg725 . PMC 212819 . PMID 12930947 .
Guo C, Fischhaber PL, Luk-Paszyc MJ, et al. (2004). "Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis" . EMBO J . 22 (24): 6621–30. doi :10.1093/emboj/cdg626 . PMC 291821 . PMID 14657033 .
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs" . Nat. Genet . 36 (1): 40–5. doi :10.1038/ng1285 . PMID 14702039 .
Ohashi E, Murakumo Y, Kanjo N, et al. (2005). "Interaction of hREV1 with three human Y-family DNA polymerases" . Genes Cells . 9 (6): 523–31. doi :10.1111/j.1356-9597.2004.00747.x . PMID 15189446 . S2CID 24470762 .
Tissier A, Kannouche P, Reck MP, et al. (2005). "Co-localization in replication foci and interaction of human Y-family members, DNA polymerase pol eta and REVl protein". DNA Repair (Amst.) . 3 (11): 1503–14. doi :10.1016/j.dnarep.2004.06.015 . PMID 15380106 .
Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4" . Nature . 434 (7034): 724–31. Bibcode :2005Natur.434..724H . doi :10.1038/nature03466 . PMID 15815621 .
Lin X, Okuda T, Trang J, Howell SB (2006). "Human REV1 modulates the cytotoxicity and mutagenicity of cisplatin in human ovarian carcinoma cells". Mol. Pharmacol . 69 (5): 1748–54. doi :10.1124/mol.105.020446 . PMID 16495473 . S2CID 22316155 .
Masuda Y, Kamiya K (2006). "Role of single-stranded DNA in targeting REV1 to primer termini" . J. Biol. Chem . 281 (34): 24314–21. doi :10.1074/jbc.M602967200 . PMID 16803901 .
Yuasa MS, Masutani C, Hirano A, et al. (2006). "A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest" . Genes Cells . 11 (7): 731–44. doi :10.1111/j.1365-2443.2006.00974.x . PMID 16824193 . S2CID 32695133 .