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Aliases RFC2, RFC40, replication factor C subunit 2
External IDs MGI: 1341868 HomoloGene: 6885 GeneCards: RFC2
RNA expression pattern
PBB GE RFC2 1053 at fs.png

PBB GE RFC2 203696 s at fs.png
More reference expression data
Species Human Mouse
RefSeq (mRNA)



RefSeq (protein)


Location (UCSC) Chr 7: 74.23 – 74.25 Mb Chr 5: 134.58 – 134.6 Mb
PubMed search [1] [2]
View/Edit Human View/Edit Mouse

Replication factor C subunit 2 is a protein that in humans is encoded by the RFC2 gene.[3][4][5]


The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.[5]


RFC2 has been shown to interact with BRD4,[6] CHTF18,[7][8] PCNA,[9][10][11] RFC4[9][12] and RFC5.[9][12]


  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ Chen M, Pan ZQ, Hurwitz J (Apr 1992). "Sequence and expression in Escherichia coli of the 40-kDa subunit of activator 1 (replication factor C) of HeLa cells". Proceedings of the National Academy of Sciences of the United States of America. 89 (7): 2516–20. doi:10.1073/pnas.89.7.2516. PMC 48692Freely accessible. PMID 1313560. 
  4. ^ Okumura K, Nogami M, Taguchi H, Dean FB, Chen M, Pan ZQ, Hurwitz J, Shiratori A, Murakami Y, Ozawa K (Jan 1995). "Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23". Genomics. 25 (1): 274–8. doi:10.1016/0888-7543(95)80135-9. PMID 7774928. 
  5. ^ a b "Entrez Gene: RFC2 replication factor C (activator 1) 2, 40kDa". 
  6. ^ Maruyama T, Farina A, Dey A, Cheong J, Bermudez VP, Tamura T, Sciortino S, Shuman J, Hurwitz J, Ozato K (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Molecular and Cellular Biology. 22 (18): 6509–20. doi:10.1128/MCB.22.18.6509-6520.2002. PMC 135621Freely accessible. PMID 12192049. 
  7. ^ Bermudez VP, Maniwa Y, Tappin I, Ozato K, Yokomori K, Hurwitz J (Sep 2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA". Proceedings of the National Academy of Sciences of the United States of America. 100 (18): 10237–42. doi:10.1073/pnas.1434308100. PMC 193545Freely accessible. PMID 12930902. 
  8. ^ Merkle CJ, Karnitz LM, Henry-Sánchez JT, Chen J (Aug 2003). "Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment". The Journal of Biological Chemistry. 278 (32): 30051–6. doi:10.1074/jbc.M211591200. PMID 12766176. 
  9. ^ a b c Cai J, Gibbs E, Uhlmann F, Phillips B, Yao N, O'Donnell M, Hurwitz J (Jul 1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme". The Journal of Biological Chemistry. 272 (30): 18974–81. doi:10.1074/jbc.272.30.18974. PMID 9228079. 
  10. ^ Pan ZQ, Chen M, Hurwitz J (Jan 1993). "The subunits of activator 1 (replication factor C) carry out multiple functions essential for proliferating-cell nuclear antigen-dependent DNA synthesis". Proceedings of the National Academy of Sciences of the United States of America. 90 (1): 6–10. doi:10.1073/pnas.90.1.6. PMC 45588Freely accessible. PMID 8093561. 
  11. ^ Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 (43): 40362–7. doi:10.1074/jbc.M206194200. PMID 12171929. 
  12. ^ a b Cai J, Yao N, Gibbs E, Finkelstein J, Phillips B, O'Donnell M, Hurwitz J (Sep 1998). "ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits". Proceedings of the National Academy of Sciences of the United States of America. 95 (20): 11607–12. doi:10.1073/pnas.95.20.11607. PMC 21688Freely accessible. PMID 9751713. 

Further reading[edit]