Talk:Hybridoma technology

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Question[edit]

The article isn't clear. THere's no enough data on what a hybridoma is (does it multiply, for example? what immortal actually means? it multiplies forever, or a specific cell doesn't die?, the cell has 43 chromosomes? if so how it multiplies?) Gabrieloncho (talk) 13:59, 5 April 2010 (UTC)

I agree. --David Tornheim (talk) 01:20, 14 February 2016 (UTC)

Discovery of hybridomas[edit]

Will someone please add in some history on the discovery of hybridomas? Here's an article: http://www.sciencemag.org/cgi/reprint/215/4536/1073 128.12.37.211 (talk) 11:55, 13 May 2008 (UTC)

Merged partly diverging descriptions of process[edit]

Text I removed because it contradicts the rest of the description, or because it is unclear:

While some may believe that IL-6 is essential for this step, it is not necessary to add that expensive supplement, rather use 50% heat-inactivated FBS for the first week.[citation needed] Add 10% FBS DMEM to the clone culture plate after screening for single colony wells.
After using HAT it is often desirable to use HT containing media.

--ἀνυπόδητος (talk) 12:39, 5 January 2010 (UTC)

Aug 2014 Edit[edit]

You will see my note in my Aug 2014 edit. I recommend that relevent content is moved to the mAb page. Also, some text was removed, but captured in higher level of detail in summary (e.g. more specific / lower threshold than staining) and the text regarding the benefit of diagnostics using mAb to avoid unnecessary procedures.

I recommend this page be evaluated by an expert and discuss 'Hybridoma technology' only. January2009 (talk) 14:40, 18 August 2014 (UTC)

Making this article more accessible[edit]

@Boghog: Thank you! Huge improvement. So is one of the main purposes of creating these anti-bodies (besides research) to make vaccines or as a drug to fight off infection of some sort? I guess I don't really understand why one would want to mass produce anti-bodies besides research. I know the human body makes anti-bodies to kill off colds and/or viruses and perhaps other pathogens, but I thought Penicillin was the only drug (a fungus I believe) that could actually kill a cold (bacteria), and that there was pretty much no medical cure for any cold stemming from a virus. So I am a little confused about how these manufactured anti-bodies can be used. --David Tornheim (talk) 11:20, 14 February 2016 (UTC)

Thanks. This is somewhat outside my area of expertise, so I hope I haven't introduced any errors. Monoclonal antibodies produced by hybridomas are used for all sorts of things. In addition to the most obvious application of helping the immune system to fight off infection, they can engineered to block the immune system in inflammatory diseases (see biopharmaceutical). Antibodies are also used in ELISA as diagnostic tools. The current article focuses on diagnostics but this article probably should be expanded to include more on the development and manufacture of therapeutics. Boghog (talk) 13:10, 14 February 2016 (UTC)