U2AF2

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U2AF2
Protein U2AF2 PDB 1jmt.png
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases U2AF2, U2AF65, U2 small nuclear RNA auxiliary factor 2
External IDs MGI: 98886 HomoloGene: 110853 GeneCards: 11338
RNA expression pattern
PBB GE U2AF2 218381 s at tn.png

PBB GE U2AF2 218382 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001012478
NM_007279

NM_001205231
NM_133671

RefSeq (protein)

NP_001012496.1
NP_009210.1

NP_001192160.1

Location (UCSC) Chr 19: 55.65 – 55.67 Mb Chr 7: 5.06 – 5.08 Mb
PubMed search [1] [2]
Wikidata
View/Edit Human View/Edit Mouse

Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene.[1]

Function[edit]

U2 auxiliary factor (U2AF), composed of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit, which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple alternatively spliced transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.[2]

In humans and other tetrapods, it has been shown that without U2AF2, the splicing process is inhibited. However, in zebrafish and other teleosts the RNA splicing process can still occur on certain genes in the absence of U2AF2. This may be because 10% of genes have alternating TG and AC base pairs at the 3' splice site (3'ss) and 5' splice site (5'ss) respectively on each intron, which alters the secondary structure of the RNA and influences splicing. [3]

Interactions[edit]

U2AF2 has been shown to interact with:

References[edit]

  1. ^ Zamore PD, Patton JG, Green MR (Mar 1992). "Cloning and domain structure of the mammalian splicing factor U2AF". Nature 355 (6361): 609–14. doi:10.1038/355609a0. PMID 1538748. 
  2. ^ "Entrez Gene: U2AF2 U2 small nuclear RNA auxiliary factor 2". 
  3. ^ Lin CL, Taggart AJ, Lim KH, Cygan KJ, Ferraris L, Creton R, Huang YT, Fairbrother WG (January 2016). "RNA structure replaces the need for U2AF2 in splicing". Genome Research 26 (1): 12–23. doi:10.1101/gr.181008.114. PMID 26566657. 
  4. ^ a b c d Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. 
  5. ^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. 
  6. ^ Berglund JA, Abovich N, Rosbash M (Mar 1998). "A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition". Genes Dev. 12 (6): 858–67. doi:10.1101/gad.12.6.858. PMC 316625. PMID 9512519. 
  7. ^ Abovich N, Rosbash M (May 1997). "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals". Cell 89 (3): 403–12. doi:10.1016/s0092-8674(00)80221-4. PMID 9150140. 
  8. ^ Zhang WJ, Wu JY (Oct 1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing". Mol. Cell. Biol. 16 (10): 5400–8. PMC 231539. PMID 8816452. 
  9. ^ Zhang WJ, Wu JY (Feb 1998). "Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing". Mol. Cell. Biol. 18 (2): 676–84. PMC 108778. PMID 9447963. 
  10. ^ Wang HY, Lin W, Dyck JA, Yeakley JM, Songyang Z, Cantley LC, Fu XD (Feb 1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells". J. Cell Biol. 140 (4): 737–50. doi:10.1083/jcb.140.4.737. PMC 2141757. PMID 9472028. 
  11. ^ Zhang M, Zamore PD, Carmo-Fonseca M, Lamond AI, Green MR (Sep 1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit". Proc. Natl. Acad. Sci. U.S.A. 89 (18): 8769–73. doi:10.1073/pnas.89.18.8769. PMC 50002. PMID 1388271. 
  12. ^ Davies RC, Calvio C, Bratt E, Larsson SH, Lamond AI, Hastie ND (Oct 1998). "WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes". Genes Dev. 12 (20): 3217–25. doi:10.1101/gad.12.20.3217. PMC 317218. PMID 9784496. 

Further reading[edit]