Mimosinase: Difference between revisions
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This enzyme belongs to the family of [[hydrolase]]s, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The [[List of enzymes|systematic name]] of this enzyme class is '''mimosine amidohydrolase'''. |
This enzyme belongs to the family of [[hydrolase]]s, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The [[List of enzymes|systematic name]] of this enzyme class is '''mimosine amidohydrolase'''. |
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== Occurrence == |
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Known to occur in all ''[[Leucaena]]'' and ''[[Mimosa]]''.<ref name = "Biotechnology"/> Negi & Borthakur 2016 [[gene cloning|clone]] the [[synthase]] found in ''[[Leucaena leucocephala|L. leucocephala]]'' .<ref name = "Biotechnology"/> |
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== Research methods == |
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[[Heterologous expression]] in ''[[E. coli]]'' can be used.<ref name = "Biotechnology"/> Negi & Borthakur 2016 provide a [[laboratory protocol|protocol]].<ref name = "Biotechnology"/> |
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==References== |
==References== |
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<ref name = "Biotechnology"> {{ Cite book | year = 2016 | publication-place = [[New York, NY]]| publisher = [[Springer Science+Business Media]] | volume = 1405 | series = [[Methods in Molecular Biology]] (MIMB) | issn = 1064-3745 | doi = 10.1007/978-1-4939-3393-8 | title = Biotechnology of Plant Secondary Metabolism | page = 59–77 }} </ref> |
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* {{cite journal | vauthors = Tangendjaja B, Lowry JB, Wills RH | date = 1986 | title = Isolation of a mimosine degrading enzyme from Leucaena leaf | journal = J. Sci. Food Agric. | volume = 37 | pages = 523–526 | doi = 10.1002/jsfa.2740370603 | issue = 6 }} |
* {{cite journal | vauthors = Tangendjaja B, Lowry JB, Wills RH | date = 1986 | title = Isolation of a mimosine degrading enzyme from Leucaena leaf | journal = J. Sci. Food Agric. | volume = 37 | pages = 523–526 | doi = 10.1002/jsfa.2740370603 | issue = 6 }} |
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Revision as of 16:51, 26 August 2023
In enzymology, a mimosinase (EC 3.5.1.61) is an enzyme that catalyzes the chemical reaction
- (S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate + H2O 3-hydroxy-4H-pyrid-4-one + L-serine
Thus, the two substrates of this enzyme are (S)-2-amino-3-(3-hydroxy-4-oxo-4H-pyridin-1-yl)propanoate and H2O, whereas its two products are 3-hydroxy-4H-pyrid-4-one and L-serine.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is mimosine amidohydrolase.
Occurrence
Known to occur in all Leucaena and Mimosa.[1] Negi & Borthakur 2016 clone the synthase found in L. leucocephala .[1]
Research methods
Heterologous expression in E. coli can be used.[1] Negi & Borthakur 2016 provide a protocol.[1]
References
- ^ a b c d Biotechnology of Plant Secondary Metabolism. Methods in Molecular Biology (MIMB). Vol. 1405. New York, NY: Springer Science+Business Media. 2016. p. 59–77. doi:10.1007/978-1-4939-3393-8. ISSN 1064-3745.
- Tangendjaja B, Lowry JB, Wills RH (1986). "Isolation of a mimosine degrading enzyme from Leucaena leaf". J. Sci. Food Agric. 37 (6): 523–526. doi:10.1002/jsfa.2740370603.
