|PDB structures||RCSB PDB PDBe PDBsum|
|Gene Ontology||AmiGO / EGO|
Acetaldehyde dehydrogenases (EC 184.108.40.206) are dehydrogenase enzymes which catalyze the conversion of acetaldehyde into acetic acid. The oxidation of acetaldehyde to acetate can be summarized as follows:
In humans, there are three known genes which encode this enzymatic activity, ALDH1A1, ALDH2, and the more recently discovered ALDH1B1 (also known as ALDH5). These enzymes are members of the larger class of aldehyde dehydrogenases.
The CAS number for this type of the enzyme is [9028-91-5].
Cysteine-302 is one of three consecutive Cys residues and is crucial to the enzyme’s catalytic function. The residue is alkylated by iodoacetamide in both the cytosolic and mitochondrial isozymes, with modifications to Cys-302 indicative of catalytic activity with other residues. Furthermore, the preceding sequence Gln-Gly-Gln-Cys is conserved in both isozymes for both human and horse, which is consistent with Cys-302 being crucial to catalytic function.
As discovered by site-directed mutagenesis, glutamate-268 is a key component of liver acetaldehyde dehydrogenase and is also critical to catalytic activity. Since activity in mutants could not be restored by addition of general bases, it’s suggested that the residue functions as a general base for activation of the essential Cys-302 residue.
In bacteria, acylating acetaldehyde dehydrogenase forms a bifunctional heterodimer with metal-dependent 4-hydroxy-2-ketovalerate aldolase. Utilized in the bacterial degradation of toxic aromatic compounds, the enzyme’s crystal structure indicates that intermediates are shuttled directly between active sites through a hydrophobic intermediary channel, providing an unreactive environment in which to move the reactive acetaldehyde intermediate from the aldolase active site to the acetaldehyde dehydrogenase active site. Such communication between proteins allows for the efficient transfer substrates from one active site to the next.
Although the two isozymes (ALDH1 and ALDH2) do not share a common subunit, the homology between the human ALDH1 and ALDH2 proteins is 66% at the coding nucleotide level and 69% at the amino acid level, which is found to be lower than the 91% homology between human ALDH1 and horse ALDH1. Such a finding is consistent with evidence suggesting the early evoluationary divergence between cytosolic and mitochondrial isozymes, as seen in the 50% homology between pig mitochondrial and cytosolic asparatate aminotransferases.
Role in metabolism of alcohol
In the liver, the enzyme alcohol dehydrogenase oxidizes ethanol into acetaldehyde, which is then further converted into the harmless acetic acid (vinegar) by acetaldehyde dehydrogenase. Acetaldehyde is more toxic than alcohol and is responsible for many hangover symptoms.
About 50% of people of Northeast Asian descent have a dominant mutation in their acetaldehyde dehydrogenase gene, making this enzyme less effective. A similar mutation is found in about 5-10% of blond-haired blue-eyed people of Northern European descent. In these people, acetaldehyde accumulates after drinking alcohol, leading to symptoms of acetaldehyde poisoning, including the characteristic flushing of the skin and increased heart and respiration rates. Other symptoms can include severe abdominal and urinary tract cramping, hot and cold flashes, profuse sweating, and profound malaise. Individuals with deficient acetaldehyde dehydrogenase activity are far less likely to become alcoholics, but seem to be at a greater risk of liver damage, alcohol-induced asthma, and contracting cancers of the oro-pharynx and esophagus due to acetaldehyde overexposure.
ALDH2, which has a lower KM for acetaldhehydes than ALDH1 and acts predominantly in the mitochondrial matrix, is the main enzyme in acetaldehyde metabolism and has three genotypes. A single point mutation (G → A) at exon 12 of the ALDH2 gene causes a replacement of glutamine with lysine at residue 487, resulting in the ALDH2K enzyme. ALDH2K has an increased KM for NAD+, rendering it virtually inactive at cellular concentrations of NAD+. Since ALDH2 is a randomized tetramer, the hetero-mutated genotype is reduced to only 6% activity compared to wild type, while homo-mutated genotypes have virtually zero enzyme activity. The ALDH2-deficient subunit is dominant in hybridization with a wild type subunit, resulting in inactivation of the isozyme by interfering with catalytic activity and increasing turnover. ALDH2 genetic variation has been closely correlated with alcohol dependence, with heterozygotes at a reduced risk compared to wild type homozygotes and individual homozygotes for the ALDH2-deficient at a very low risk for alcoholism.
The drug disulfiram (Antabuse) prevents the oxidation of acetaldehyde to acetic acid and is used in the treatment of alcoholism. ALDH1 is strongly inhibited by disulfiram, while ALDH2 is resistant to its effect. The cysteine residue at 302 in ALDH1 and 200 in ALDH2 is implicated as a disulfiram binding site on the enzyme and serves as a disfulfiram sensitive thiol site. Covalent binding of disulfiram to the thiol blocks the binding of one of the cysteine residues with iodoacetamide, thereby inactivating the enzyme and significantly lowering catalytic activity. Activity can be recovered by treatment with 2-mercaptoethanol, although not with glutathione.
Metronidazole (Flagyl), which is used to treat certain parasitic infections as well as pseudomembranous colitis, causes similar effects to disulfiram. Coprine (which is an amino acid found in certain coprinoid mushrooms) metabolizes in vivo to 1-aminocyclopropanol which causes similar effects as well.
Role in fat metabolism
ALDH1 is involved in the metabolism of Vitamin A. Animal models suggest that absence of the gene is associated with protection against visceral adiposity (PMC2233696).
- PDB 1NVM; Manjasetty BA, Powlowski J, Vrielink A (June 2003). "Crystal structure of a bifunctional aldolase-dehydrogenase: sequestering a reactive and volatile intermediate". Proc. Natl. Acad. Sci. U.S.A. 100 (12): 6992–6997. doi:10.1073/pnas.1236794100. PMC 165818. PMID 12764229.
- Hempel J, Kaiser R, Jornvall H. (1985). "Mitochondrial aldehyde dehydrogenase from human liver". Eur. J. Biochem. 153 (1): 13–28. doi:10.1111/j.1432-1033.1985.tb09260.x. PMID 4065146.
- Wang X, Weiner H. (1995). "Involvement of glutamate 268 in the active site of human liver mitochondrial (class 2) aldehyde dehydrogenase as probed by site-directed mutagenesis". Biochemistry 34 (JAN 10): 237–243. doi:10.1021/bi00001a028. PMID 7819202.
- Hsu L.C., Tani K, Fujiyoshi T, Kurachi K, Yoshida A. (1985). "Cloning of cDNAs for human aldehyde dehydrogenases 1 and 2". Porc. Natl. Acad. Sci. USA 82 (JUNE): 3771–3775. doi:10.1073/pnas.82.11.3771. PMC 397869. PMID 2987944.
- Xiao Q, Weiner H, Crabb DW (1996). "The mutation in the mitochondrial aldehyde dehydrogenase (ALDH2) gene responsible for alcohol-induced flushing increases turnover of the enzyme tetramers in a dominant fashion". J. Clin. Invest. 98 (9): 2027–2032. doi:10.1172/JCI119007. PMC 507646. PMID 8903321.
- Crabb D, Xiao Q. (2006). "Studies on the Enzymology of Aldehyde Dehydrogenase-2 in Genetically Modified HeLa Cells". Alcoholism: Clinical and Experimental Research 22 (4): 780–781. doi:10.1111/j.1530-0277.1998.tb03867.x. PMID 9660300.
- Lu Y, Morimoto K. (2009). "Is habitual alcohol drinking associated with reduced electrophoretic DNA migration in peripheral blood leukocytes from ALDH2-deficient male Japanese?". Mutagenesis. 24 (4): 303–308. doi:10.1093/mutage/gep008. PMID 19286920.
- Macgregor S, Lind P, Bucholz K, Hansell N, Madden P, Richter M, Montgomery G, Martin N, Heath A, Whitfield J. (2009). "Associations of ADH and ALDH2 gene variation with self report alcohol reactions, consumption and dependence: an integrated analysis". Human Molecular Genetics 18 (3): 580–593. doi:10.1093/hmg/ddn372. PMC 2722191. PMID 18996923.
- Lind PA, Eriksson CJ, Wilhelmsen KC. (2008). "The role of aldehyde dehydrogenase-1 (ALDH1A1) polymorphisms in harmful alcohol consumption in a Finnish population". Human Genomics 3 (1): 24–35. PMID 19129088.
- Hempel J, Bahr-Lindstrom, Jornvall Hans. (1985). "Aldehyde dehydrogenase from human liver". European Journal of Biochemistry 141 (1): 21–35. doi:10.1111/j.1432-1033.1984.tb08150.x. PMID 6723659.
- Vallari RC, Pietruszko R. (1982). "Human aldehyde dehydrogenase: mechanism of inhibition of disulfiram". Science 216 (4546): 637–639. doi:10.1126/science.7071604. PMID 7071604.