This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminalLIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1 and 2) have C-terminal LIM domains.
^Arimura T, Hayashi T, Terada H, Lee SY, Zhou Q, Takahashi M, Ueda K, Nouchi T, Hohda S, Shibutani M, Hirose M, Chen J, Park JE, Yasunami M, Hayashi H, Kimura A (February 2004). "A Cypher/ZASP mutation associated with dilated cardiomyopathy alters the binding affinity to protein kinase C". J. Biol. Chem.279 (8): 6746–52. doi:10.1074/jbc.M311849200. PMID14660611.
Marziliano N, Mannarino S, Nespoli L et al. (2007). "Barth syndrome associated with compound hemizygosity and heterozygosity of the TAZ and LDB3 genes". Am. J. Med. Genet. A143 (9): 907–15. doi:10.1002/ajmg.a.31653. PMID17394203.
Klaavuniemi T, Ylänne J (2006). "Zasp/Cypher internal ZM-motif containing fragments are sufficient to co-localize with alpha-actinin--analysis of patient mutations". Exp. Cell Res.312 (8): 1299–311. doi:10.1016/j.yexcr.2005.12.036. PMID16476425.
Arimura T, Hayashi T, Terada H et al. (2004). "A Cypher/ZASP mutation associated with dilated cardiomyopathy alters the binding affinity to protein kinase C". J. Biol. Chem.279 (8): 6746–52. doi:10.1074/jbc.M311849200. PMID14660611.
Frey N, Olson EN (2002). "Calsarcin-3, a novel skeletal muscle-specific member of the calsarcin family, interacts with multiple Z-disc proteins". J. Biol. Chem.277 (16): 13998–4004. doi:10.1074/jbc.M200712200. PMID11842093.
Zhou Q, Ruiz-Lozano P, Martone ME, Chen J (1999). "Cypher, a striated muscle-restricted PDZ and LIM domain-containing protein, binds to alpha-actinin-2 and protein kinase C". J. Biol. Chem.274 (28): 19807–13. doi:10.1074/jbc.274.28.19807. PMID10391924.
Ishikawa K, Nagase T, Suyama M et al. (1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res.5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID9734811.
Lanfranchi G, Muraro T, Caldara F et al. (1996). "Identification of 4370 expressed sequence tags from a 3'-end-specific cDNA library of human skeletal muscle by DNA sequencing and filter hybridization". Genome Res.6 (1): 35–42. doi:10.1101/gr.6.1.35. PMID8681137.