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Proteins are broken down in the stomach during digestion by enzymes known as proteases into smaller polypeptides to provide amino acids for the organism, including the essential amino acids that the organism cannot biosynthesize itself. Aside from their role in protein synthesis, amino acids are also important nutritional sources of nitrogen.

Proteins, like carbohydrates, contain 4 kilocalories per gram as opposed to lipids which contain 9 kilocalories and alcohols which contain 7 kilocalories. The liver and to a much lesser extent the kidneys, can convert amino acids used by cells in protein biosynthesis into glucose by a process known as gluconeogenesis. The amino acids leucine and lysine are exceptions.

Sources of proteins

Dietary sources of protein include meats, eggs, grains, legumes, and dairy products such as milk and cheese.^[1] Of the over 20 amino acids used by humans, 12 nonessential amino acids can be synthesized by the body, and are not required in the diet (though there are exceptions for some in special cases). The 9 essential amino acids, however, cannot be created by the body and must come from dietary sources.

Most animal sources and certain vegetable sources have the complete complement of all 9 essential amino acids. However, it is not necessary to consume a single food source that contains all the essential amino acids, as long as all the essential amino acids are eventually present in the diet: see complete protein and protein combining.

Protein quality

Different proteins have different levels of biological availability to the human body. Many methods have been introduced to measure protein utilization and retention rates in humans. They include biological value, Net Protein Utilization or NPU, and PDCAAS (Protein Digestibility Corrected Amino Acids Score) which was developed by the FDA as an improvement over the Protein Efficiency Ratio (PER) method. These methods examine which proteins are most efficiently used by the body. In general they conclude that animal complete proteins that contain all the essential amino acids such as milk, eggs, and meat, and the complete vegetable protein soy are of most value to the body. ^[2].

Egg whites have been determined to have the standard biological value of 100 (though some sources may have biological values higher), which means that most of the absorbed nitrogen from egg white protein can be retained and used by the body. Since the amino acids found in plants are biologically different from those found in humans and animals, the biological value of plant protein sources is considerably lower. For example, corn has a BA of 70 while peanuts have a relatively low BA of 40. ^[3]

Digestion of protein

Digestion typically begins in the stomach when pepsinogen is converted to pepsin by the action of hydrochloric acid, and continued by trypsin and chymotrypsin in the intestine. The amino acids and their derivatives into which dietary protein is degraded are then absorbed by the gastrointestinal tract. The absorption rates of individual amino acids are highly dependent on the protein source; for example, the digestibilities of many amino acids in humans differ between soy and milk proteins^[4] and between individual milk proteins, beta-lactoglobulin and casein.^[5] For milk proteins, about 50% of the ingested protein is absorbed between the stomach and the jejunum and 90% is absorbed by the time the digested food reaches the ileum.^[6] Biological value (BV) is a measure of the proportion of absorbed protein from a food which becomes incorporated into the proteins of the organism's body.

Dietary requirements

According to the recently updated Dietary Reference Intake guidelines, women aged 19–70 need to consume 46 grams of protein per day, while men aged 19–70 need to consume 56 grams of protein per day to avoid a deficiency.^[7] The difference is due to the fact that men's bodies generally have more muscle mass than those of women, or this may be attributed to weight difference by taking 0.8 g(of protein)/kg of body weight.

Because the body is continually breaking down protein from tissues, even adults who do not fall into the above categories need to include adequate protein in their diet every day. If enough energy is not taken in through diet, the body will use protein from the muscle mass to meet its energy needs, leading to muscle wasting over time.

Other recommendations suggest 0.8 gram of protein per kilogram of bodyweight per day while other sources suggest that higher intakes of 1-1.4 grams of protein per kilogram of bodyweight for enhanced athletes or those with a large muscle mass.^[8]

How much protein needed in a person's daily diet is determined in large part by overall energy intake, as well as by the body's need for nitrogen and essential amino acids. Physical activity and exertion as well as enhanced muscular mass increase the need for protein. Requirements are also greater during childhood for growth and development, during pregnancy or when breast-feeding in order to nourish a baby, or when the body needs to recover from malnutrition or trauma or after an operation.

Protein deficiency

Protein deficiency in developing countries

Protein deficiency is a serious cause of ill health and death in developing countries. Protein deficiency plays a part in the disease kwashiorkor. War, famine, overpopulation and other factors can increase rates of malnutrition and protein deficiency. Protein deficiency can lead to reduced intelligence or mental retardation, see deficiency in proteins, fats, carbohydrates.

In countries that suffer from widespread protein deficiency, food is generally full of plant fibers, which makes adequate energy and protein consumption very difficult. Symptoms of kwashiorkor include apathy, diarrhea, inactivity, failure to grow, flaky skin, fatty liver, and edema of the belly and legs. This edema is explained by the normal functioning of proteins in fluid balance and lipoprotein transport. ^[9] ^[10]

Dr. Latham, director of the Program in International Nutrition at Cornell University claims that malnutrition is a frequent cause of death and disease in third world countries. Protein-energy malnutrition (PEM) affects 500 million people and kills 10 million annually. In severe cases white blood cell numbers decline and the ability of leukocytes to fight infection decreases. ^[11]

Protein deficiency in developed countries

Protein deficiency is rare in developed countries but small numbers of people have difficulty getting sufficient protein due to poverty. Protein deficiency can also occur in developed countries in people who are dieting or crash dieting to lose weight, or in older adults, who may have a poor diet. Convalescent people recovering from surgery, trauma, or illness may become protein deficient if they do not increase their intake to support their increased needs. A deficiency can also occur if the protein a person eats is incomplete and fails to supply all the essential amino acids.

Excess protein consumption

Because the body is unable to store in the form of protein, excess consumed protein is broken down and converted into sugars or fatty acids. The liver removes nitrogen from the amino acids, so that they can be burned as fuel, and the nitrogen is incorporated into urea, the substance that is excreted by the kidneys. These organs can normally cope with any extra workload but if kidney disease occurs, a decrease in protein will often be prescribed.^[12]

Many researchers think excessive intake of protein forces increased calcium excretion. If there is to be excessive intake of protein, it is thought that a regular intake of calcium would be able to stabilize, or even increase the uptake of calcium by the small intestine, which would be more beneficial in older women.^[13]

Proteins are often progenitors in allergies and allergic reactions to certain foods. This is because the structure of each form of protein is slightly different; some may trigger a response from the immune system while others remain perfectly safe. Many people are allergic to casein, the protein in milk; gluten, the protein in wheat and other grains; the particular proteins found in peanuts; or those in shellfish or other seafoods.

Testing for protein in foods

The classic assay for protein concentration in food is the Kjeldahl method. This test determines the total nitrogen in a sample. The only major component of most food which contains nitrogen is protein (fat, carbohydrate and dietary fibre do not contain nitrogen). If the amount of nitrogen is multiplied by a factor depending on the kinds of protein expected in the food the total protein can be determined. On food labels the protein is given by the nitrogen multiplied by 6.25, because the average nitrogen content of proteins is about 16%. The Kjeldahl test is used because it is the method the AOAC International has adopted and is therefore used by many food standards agencies around the world.

References

^ USDA National Nutrient Database for Standard Reference, Release 20, United States Department of Agriculture. Last modified on September 26, 2007.
^ Protein Quality Comparison Chart. Retrieved from the Whey Protein Institute on 2008-05-24.
^ Wardlaw, Gordon M. Perspectives in Nutrition. Boston: McGraw-Hill Higher Education. doi:pp. 259,260. ISBN 0-07-282750-5. {{cite book}}: Check |doi= value (help)
^ Gaudichon C, Bos C, Morens C, Petzke KJ, Mariotti F, Everwand J, Benamouzig R, Dare S, Tome D, Metges CC. (2002). Ileal losses of nitrogen and amino acids in humans and their importance to the assessment of amino acid requirements. Gastroenterology 123(1):50-9.
^ Mahe S, Roos N, Benamouzig R, Davin L, Luengo C, Gagnon L, Gausserges N, Rautureau J, Tome D. (1996). Gastrojejunal kinetics and the digestion of [15N]beta-lactoglobulin and casein in humans: the influence of the nature and quantity of the protein. Am J Clin Nutr 63(4):546-52.
^ Mahe S, Marteau P, Huneau JF, Thuillier F, Tome D. (1994). Intestinal nitrogen and electrolyte movements following fermented milk ingestion in man. Br J Nutr 71(2):169-80.
^ Dietary Reference Intakes: Macronutrients. Institute of Medicine. Retrieved on 2008-05-18
^ Tarnopolsky MA, Atkinson SA, MacDougall JD, Chesley A, Phillips S, Schwarcz HP. "Evaluation of protein requirements for trained strength athletes". Journal of Applied Physiology 1992 Nov; 73 (5): 1986-1995. PMID 1474076
^ Jeffery Schwartz; Bryant, Carol A.; DeWalt, Kathleen Musante; Anita Courtney (2003). The cultural feast: an introduction to food and society. Belmont, CA: Thomson/Wadsworth. doi:pp. 282,283. ISBN 0-534-52582-2. {{cite book}}: Check |doi= value (help)CS1 maint: multiple names: authors list (link)
^ "Apache Tomcat/6.0.14 - Error report". Retrieved 2008-03-11.
^ http://www.religion-online.org/showarticle.asp?title=1405
^ Born, Steve. "Fueling For Endurance: Ten Mistakes Endurance Athletes Make and How You Can Avoid Them". UltraCycling Magazine.
^ Kerstetter, J. E., O'Brien, K. O., Caseria, D.M, Wall, D. E. & Insogna, K. L (2005) "The impact of dietary protein on calcium absorption and kinetic measures of bone turnover in women". J Clin Endocrinol Metab (2005) Vol 90, p26–31. PMID 15546911

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 ==Sources of proteins==
-Dietary sources of protein include [[meat]]s, [[egg (food)|eggs]], [[grain]]s, [[legume]]s, and [[dairy product]]s such as [[milk]] and [[cheese]].<ref name="USDANutrients">''[http://www.ars.usda.gov/Main/docs.htm?docid=15869 USDA National Nutrient Database for Standard Reference, Release 20]'', United States Department of Agriculture. Last modified on September 26, 2007.</ref> Of the 20 amino acids used by humans, the 12 nonessential amino acids can be synthesized by the body, and are not required in the diet. The 9 essential amino acids, however, cannot be created by the body and must come from dietary sources.
+Dietary sources of protein include [[meat]]s, [[egg (food)|eggs]], [[grain]]s, [[legume]]s, and [[dairy product]]s such as [[milk]] and [[cheese]].<ref name="USDANutrients">''[http://www.ars.usda.gov/Main/docs.htm?docid=15869 USDA National Nutrient Database for Standard Reference, Release 20]'', United States Department of Agriculture. Last modified on September 26, 2007.</ref> Of the over 20 amino acids used by humans, 12 nonessential amino acids can be synthesized by the body, and are not required in the diet (though there are exceptions for some in special cases). The 9 essential amino acids, however, cannot be created by the body and must come from dietary sources.
-<!-- 12+9 = 21? someone correct this please -->
 Most animal sources and certain vegetable sources have the complete complement of all 9 essential amino acids. However, it is not necessary to consume a single food source that contains all the essential amino acids, as long as all the essential amino acids are eventually present in the diet: see [[complete protein]] and [[protein combining]].