The 3 substrates of this enzyme are (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al, NAD+, and H2O, whereas its 2 products are (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate, NADH, and H+. This enzyme participates in bile acid biosynthesis.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-al:NAD+ 26-oxidoreductase. Other names in common use include:
^Masui T, Herman R, Staple E (March 1966). "The oxidation of 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha, 26-tetraol to 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol-26-oic acid via 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol-26-al by rat liver". Biochim. Biophys. Acta117 (1): 266–8. doi:10.1016/0304-4165(66)90177-2. PMID5914340.