Tryptic soy broth
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Tryptic soy broth or Trypticase soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic and facultative anaerobic bacteria. It is a general purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (i.e., they are fastidious).[1]
Uses
Sterility test medium in USP and EP as well as for inocula preparation for CLSI standards.[1]
TSB is frequently used in commercial diagnostics in conjunction with the additive sodium thioglycolate which promotes growth of anaerobes.[2]
Preparation
To prepare 1 liter of TSB, the following ingredients are dissolved under gentle heat. Adjustments to pH should be made using 1N HCl or 1N NaOH to reach a final target pH of 7.3 ± 0.2 at 25°C. The solution is then autoclaved for 15 minutes at 121°C.[3]
Tryptic Soy Agar contains per liter:
- 17 g pancreatic digest of casein
- 3 g peptic digest of soybean
- 5 g sodium chloride
- 2.5g dipotassium phosphate (K2HPO4)
- 2.5g glucose
References
- ^ a b "Tryptic soy broth". Retrieved 30 Nov 2023.
- ^ Doyle, JE; Mehrhof, WH; Ernst, RR (1968). "Limitations of thioglycolate broth as a sterility test medium for materials exposed to gaseous ethylene oxide". Appl Microbiol. 16 (11): 1742–4. doi:10.1128/am.16.11.1742-1744.1968. PMC 547751. PMID 4973064.
- ^ "BAM Media M154: Trypticase (Tryptic) Soy Broth". Food and Drug Administration. January 2001. Retrieved 18 April 2015.
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