A chemostat (from chemical environment is static) is a bioreactor to which fresh medium is continuously added, while culture liquid containing left over nutrients, metabolic end products and microorganisms are continuously removed at the same rate to keep the culture volume constant. By changing the rate with which medium is added to the bioreactor the specific growth rate of the microorganism can be easily controlled within limits.
One of the most important features of chemostats is that microorganisms can be grown in a physiological steady state under constant environmental conditions. In this steady state, growth occurs at a constant specific growth rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.). In addition, environmental conditions can be controlled by the experimenter. Microorganisms growing in chemostats usually reach a steady state because of a negative feedback between growth rate and nutrient consumption: if a low number of cells are present in the bioreactor, the cells can grow at growth rates higher than the dilution rate as they consume little nutrient so growth is less limited by the addition of limiting nutrient with the inflowing fresh medium. The limiting nutrient is a nutrient essential for growth, present in the medium at a limiting concentration (all other nutrients are usually supplied in surplus). However, the higher the number of cells becomes, the more nutrient is consumed, lowering the concentration of the limiting nutrient. In turn, this will reduce the specific growth rate of the cells which will lead to a decline in the number of cells as they keep being removed from the system with the outflow. This results in a steady state. Due to the self-regulation, the steady state is stable. This enables the experimenter to control the specific growth rate of the microorganisms by changing the speed of the pump feeding fresh medium into the vessel.
Another important feature of chemostats and other continuous culture systems is that they are well-mixed so that environmental conditions are homogenous or uniform and microorganisms are randomly dispersed and encounter each other randomly. Therefore, competition and other interactions in the chemostat are global, in contrast to biofilms.
The rate of nutrient exchange is expressed as the dilution rate(D).At steady state, the specific growth rate (μ) of the micro-organism is equal to the dilution rate (D). The dilution rate is defined as the flow of medium per time (F) over the volume of culture (V) in the bioreactor
Maximal growth rate and critical dilution rate
Specific growth rate (μ) is inversely related to the time it takes the biomass to double called doubling time (td) by:
μ = ln(2)/td
Therefore, the doubling time (td) becomes a function of dilution rate (D) in steady state:
td = ln(2)/D
Each microorganism growing on a particular substrate has a maximal specific growth rate (μmax) (the rate of growth observed if growth is limited by internal constraints rather than external nutrients). If a dilution rate is chosen that is higher than μmax, the cells cannot grow at a rate as fast as the rate with which they are being removed so the culture will not be able to sustain itself in the bioreactor, and will wash out.
However, since the concentration of the limiting nutrient in the chemostat cannot exceed the concentration in the feed, the specific growth rate that the cells can reach in the chemostat is usually slightly lower than the maximal specific growth rate because specific growth rate usually increases with nutrient concentration as described by Monod kinetics. The highest specific growth rates (μmax) cells can attain is equal to the critical dilution rate (Dc):
where S is substrate or nutrient concentration in the chemostat and KS is the half-saturation constant (this equation assumes Monod kinetics).
Chemostats in research are used for investigations in cell biology, as a source for large volumes of uniform cells or protein. The chemostat is often used to gather steady state data about an organism in order to generate a mathematical model relating to its metabolic processes. Chemostats are also used as microcosms in ecology and evolutionary biology. In the one case, mutation/selection is a nuisance, in the other case, it is the desired process under study. Chemostats can also be used to enrich for specific types of bacterial mutants in culture such as auxotrophs or those that are resistant to antibiotics or bacteriophages for further scientific study.
Competition for single and multiple resources, the evolution of resource acquisition and utilization pathways, cross-feeding/symbiosis, antagonism, predation, and competition among predators have all been studied in ecology and evolutionary biology using chemostats.
- Foaming results in overflow with the volume of liquid not exactly constant.
- Some very fragile cells are ruptured during agitation and aeration.
- Cells may grow on the walls or adhere to other surfaces, which may be overcome by treating the glass walls of the vessel with a silane to render them hydrophobic. However, cells will be selected for attachment to the walls since those that do will not be removed from the system. Those bacteria that stick firmly to the walls forming a biofilm are difficult to study under chemostat conditions.
- Mixing may not truly be uniform, upsetting the "static" property of the chemostat.
- Dripping the media into the chamber actually results in small pulses of nutrients and thus oscillations in concentrations, again upsetting the "static" property of the chemostat.
- Bacteria travel upstream quite easily. They will reach the reservoir of sterile medium quickly unless the liquid path is interrupted by an air break in which the medium falls in drops through air.
Continuous efforts to remedy each defect lead to variations on the basic chemostat quite regularly. Examples in the literature are numerous.
- Antifoaming agents are used to suppress foaming.
- Agitation and aeration can be done gently.
- Many approaches have been taken to reduce wall growth
- Various applications use paddles, bubbling, or other mechanisms for mixing
- Dripping can be made less drastic with smaller droplets and larger vessel volumes
- Many improvements target the threat of contamination
Fermentation setups closely related to the chemostats are the turbidostat, the auxostat and the retentostat. In retentostats, culture liquid is also removed from the bioreactor, but a filter retains the biomass. In this case, the biomass concentration increases until the nutrient requirement for biomass maintenance has become equal to the amount of limiting nutrient that can be consumed.
- Bacterial growth
- Biochemical engineering
- Continuous stirred-tank reactor (CSTR)
- E. coli long-term evolution experiment
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- A final thesis including mathematical models of the chemostat and other bioreactors
- A page about one laboratory chemostat design
- Comprehensive chemostat manual (Dunham lab). Procedures and principles are general.