Cholate—CoA ligase

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Cholate—CoA ligase
EC no.
CAS no.9027-90-1
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum

Cholate—CoA ligase (EC, BAL, bile acid CoA ligase, bile acid coenzyme A ligase, choloyl-CoA synthetase, choloyl coenzyme A synthetase, cholic thiokinase, cholate thiokinase, cholic acid:CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl coenzyme A synthetase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate-CoA synthetase, THCA-CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate—CoA ligase, 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanate:CoA ligase (AMP-forming), cholyl-CoA synthetase, trihydroxycoprostanoyl-CoA synthetase) is an enzyme with systematic name cholate:CoA ligase (AMP-forming).[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction

(1) ATP + cholate + CoA AMP + diphosphate + choloyl-CoA
(2) ATP + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA AMP + diphosphate + (25R)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA

This enzyme requires Mg2+ for activity.


  1. ^ Elliott WH (March 1956). "The enzymic activation of cholic acid by guinea-pig-liver microsomes". The Biochemical Journal. 62 (3): 427–33. PMC 1215932. PMID 13303991.
  2. ^ Elliott WH (February 1957). "The breakdown of adenosine triphosphate accompanying cholic acid activation by guinea-pig liver microsomes". The Biochemical Journal. 65 (2): 315–21. PMC 1199872. PMID 13403911.
  3. ^ Prydz K, Kase BF, Björkhem I, Pedersen JI (August 1988). "Subcellular localization of 3 alpha, 7 alpha-dihydroxy- and 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoyl-coenzyme A ligase(s) in rat liver". Journal of Lipid Research. 29 (8): 997–1004. PMID 3183523.
  4. ^ Schepers L, Casteels M, Verheyden K, Parmentier G, Asselberghs S, Eyssen HJ, Mannaerts GP (January 1989). "Subcellular distribution and characteristics of trihydroxycoprostanoyl-CoA synthetase in rat liver". The Biochemical Journal. 257 (1): 221–9. PMC 1135559. PMID 2521999.
  5. ^ Mallonee DH, Adams JL, Hylemon PB (April 1992). "The bile acid-inducible baiB gene from Eubacterium sp. strain VPI 12708 encodes a bile acid-coenzyme A ligase". Journal of Bacteriology. 174 (7): 2065–71. PMC 205821. PMID 1551828.
  6. ^ Wheeler JB, Shaw DR, Barnes S (December 1997). "Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes". Archives of Biochemistry and Biophysics. 348 (1): 15–24. doi:10.1006/abbi.1997.0391. PMID 9390170.
  7. ^ Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S (December 2002). "Molecular cloning and expression of rat liver bile acid CoA ligase". Journal of Lipid Research. 43 (12): 2062–71. doi:10.1194/jlr.M200260-JLR200. PMID 12454267.

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