Janus Green B

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Janus Green B[1]
Janus green B.svg
Ball-and-stick model of the component ions of Janus Green B
IUPAC name
8-(4-Dimethylaminophenyl)diazenyl-N,N-diethyl-10-phenylphenazin-10-ium-2-amine chloride
Other names
Diazin Green S
Union Green B
3D model (JSmol)
ECHA InfoCard 100.018.814
EC Number 220-695-6
Molar mass 511.06 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900.[2]

The indicator Janus Green B changes colour according to the amount of oxygen present.[3] When oxygen is present, the indicator oxidizes to a blue colour. In the absence of oxygen, the indicator is reduced and changes to a pink colour.


  1. ^ Janus Green B, at Sigma-Aldrich
  2. ^ Michaelis, L. (1900). Die vitale Farbung, eine Darstellungsmethode der Zellgranula. Archiv für mikroskopische Anatomie 55:558-575(Also Tafel XXXII)
  3. ^ http://www.mrothery.co.uk/bio_web_prac/practicals/2anaerobic%20resp%20student.doc