NDUFA8

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NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa
Identifiers
Symbols NDUFA8 ; CI-19KD; CI-PGIV; PGIV
External IDs OMIM603359 MGI1915625 HomoloGene40932 ChEMBL: 3294 GeneCards: NDUFA8 Gene
EC number 1.6.5.3, 1.6.99.3
RNA expression pattern
PBB GE NDUFA8 218160 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 4702 68375
Ensembl ENSG00000119421 ENSMUSG00000026895
UniProt P51970 Q9DCJ5
RefSeq (mRNA) NM_014222 NM_026703
RefSeq (protein) NP_055037 NP_080979
Location (UCSC) Chr 9:
122.14 – 122.16 Mb
Chr 2:
36.04 – 36.05 Mb
PubMed search [1] [2]

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 is an enzyme that in humans is encoded by the NDUFA8 gene.[1] The NDUFA8 protein is a subunit of NADH dehydrogenase (ubiquinone), which is located in the mitochondrial inner membrane and is the largest of the five complexes of the electron transport chain.[2][3]

Structure[edit]

The NDUFA8 gene is located on the q arm of chromosome 9 in position 33.2 and spans 27,354 base pairs.[1] The gene produces a 20 kDa protein composed of 172 amino acids.[4] NDUFA8 is a subunit of the enzyme NADH dehydrogenase (ubiquinone), the largest of the respiratory complexes. The structure is L-shaped with a long, hydrophobic transmembrane domain and a hydrophilic domain for the peripheral arm that includes all the known redox centers and the NADH binding site.[2] It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of Complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and that the hydrophobic domain acts as an anchor for the NADH dehydrogenase (ubiquinone) complex at the inner mitochondrial membrane. NDUFA8 is one of about 31 hydrophobic subunits that form the transmembrane region of Complex I. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.[1][3][5][6]

Function[edit]

The human NDUFA8 gene codes for a subunit of Complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone.[1] NADH binds to Complex I and transfers two electrons to the isoalloxazine ring of the flavin mononucleotide (FMN) prosthetic arm to form FMNH2. The electrons are transferred through a series of iron-sulfur (Fe-S) clusters in the prosthetic arm and finally to coenzyme Q10 (CoQ), which is reduced to ubiquinol (CoQH2). The flow of electrons changes the redox state of the protein, resulting in a conformational change and pK shift of the ionizable side chain, which pumps four hydrogen ions out of the mitochondrial matrix.[2]

References[edit]

  1. ^ a b c d "Entrez Gene: NDUFA8 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8". 
  2. ^ a b c Pratt, Donald Voet, Judith G. Voet, Charlotte W. (2013). "18". Fundamentals of biochemistry : life at the molecular level (4th ed.). Hoboken, NJ: Wiley. pp. 581–620. ISBN 9780470547847. 
  3. ^ a b Emahazion T, Beskow A, Gyllensten U, Brookes AJ (Nov 1998). "Intron based radiation hybrid mapping of 15 complex I genes of the human electron transport chain". Cytogenet Cell Genet 82 (1-2): 115–9. doi:10.1159/000015082. PMID 9763677. 
  4. ^ "NDUFA8 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB). 
  5. ^ Emahazion T, Brookes AJ (Nov 1998). "Mapping of the NDUFA2, NDUFA6, NDUFA7, NDUFB8, and NDUFS8 electron transport chain genes by intron based radiation hybrid mapping". Cytogenet Cell Genet 82 (1–2): 114. doi:10.1159/000015081. PMID 9763676. 
  6. ^ Ton C, Hwang DM, Dempsey AA, Liew CC (Jan 1998). "Identification and primary structure of five human NADH-ubiquinone oxidoreductase subunits". Biochem Biophys Res Commun 241 (2): 589–94. doi:10.1006/bbrc.1997.7707. PMID 9425316. 

Further reading[edit]

This article incorporates text from the United States National Library of Medicine, which is in the public domain.