Nanobacterium (// NAN-oh-bak-TEER-ee-əm, pl. nanobacteria // NAN-oh-bak-TEER-ee-ə) is the unit or member name of a proposed class of living organisms, specifically cell-walled microorganisms with a size much smaller than the generally accepted lower limit for life (about 200 nm for bacteria, like mycoplasma). Originally based on observed nano-scale structures in geological formations (including one meteorite), the status of nanobacteria has been controversial, with some researchers suggesting they are a new class of living organism capable of incorporating radiolabeled uridine, and others attributing to them a simpler, abiotic nature. One skeptic dubbed them "the cold fusion of microbiology", in reference to a notorious episode of supposed erroneous science. The term "calcifying nanoparticles" (CNPs) has also been used as a conservative name regarding their possible status as a life form.
Research tends to agree that these structures exist, and appear to replicate in some way. However, the idea that they are living entities has now largely been discarded, and the particles are instead thought to be nonliving crystallizations of minerals and organic molecules.
In 1981 Torella and Morita described very small cells called ultramicrobacteria. Defined as being smaller than 300 nm, by 1982 MacDonell and Hood found that some could pass through a 200 nm membrane. Early in 1989, geologist Robert L. Folk found what he later identified as nannobacteria (written with double "n"), that is, nanoparticles isolated from geological specimens in travertine from hot springs of Viterbo, Italy. Initially searching for a bacterial cause for travertine deposition, scanning electron microscope examination of the mineral where no bacteria were detectable revealed extremely small objects which appeared to be biological. His first oral presentation elicited what he called "mostly a stony silence", at the 1992 Geological Society of America's annual convention. He proposed that nanobacteria are the principal agents of precipitation of all minerals and crystals on Earth formed in liquid water, that they also cause all oxidation of metals, and that they are abundant in many biological specimens.
In 1996, NASA scientist David McKay published a study suggesting the existence of nanofossils — fossils of Martian nanobacteria — in ALH84001, a meteorite originating from Mars and found in Antarctica.
Nanobacterium sanguineum was proposed in 1998 as an explanation of certain kinds of pathologic calcification (apatite in kidney stones) by Finnish researcher Olavi Kajander and Turkish researcher Neva Ciftcioglu, working at the University of Kuopio in Finland. According to the researchers the particles self-replicated in microbiological culture, and the researchers further reported having identified DNA in these structures by staining.
A paper published in 2000 by a team led by an NIH scientist John Cisar further tested these ideas. It stated that what had previously been described as "self-replication" was a form of crystalline growth. The only DNA detected in his specimens was identified as coming from the bacteria Phyllobacterium mysinacearum, which is a common contaminant in PCR reactions.
In 2004 a Mayo Clinic team led by Franklin Cockerill, John Lieske, and Virginia M. Miller, reported to have isolated nanobacteria from diseased human arteries and kidney stones. Their results were published in 2004 and 2006 respectively. Similar findings were obtained in 2005 by László Puskás at the DNA Lab, University of Szeged, Hungary. Dr. Puskás identified these particles in cultures obtained from human atherosclerotic aortic walls and blood samples of atherosclerotic patients but the group was unable to detect DNA in these samples.
In 2005, Ciftcioglu and her research team at NASA used a rotating cell culture flask, which simulates some aspects of low-gravity conditions, to culture nanobacteria suspected of rapidly forming kidney stones in astronauts. In this environment, they were found to multiply five times faster than in normal Earth gravity. The study concluded that nanobacteria might have a potential role in forming kidney stones and may need to be screened for in crews pre-flight.
The February 2008 Public Library of Science (PLoS) Pathogens article focused on the comprehensive characterization of nanobacteria. The authors say that their results rule out the existence of nanobacteria as living entities and that they are instead a unique self-propagating entity, namely self-propagating mineral-fetuin complexes.
An April 2008 Proceedings of the National Academy of Sciences (PNAS) article also reported that blood nanobacteria are not living organisms and stated that "CaCO3 precipitates prepared in vitro are remarkably similar to purported nanobacteria in terms of their uniformly sized, membrane-delineated vesicular shapes, with cellular division-like formations and aggregations in the form of colonies." The growth of such "biomorphic" inorganic precipitates was studied in detail in a 2009 Science paper, which showed that unusual crystal growth mechanisms can produce witherite precipitates from barium chloride and silica solutions that closely resemble primitive organisms. The authors commented on the close resemblance of these crystals to putative nanobacteria, stating that their results showed that evidence for life cannot rest on morphology alone.
Further work on the importance of nanobacteria in geology by R. L. Folk and co-workers includes study of calcium carbonate Bahama ooids, silicate clay minerals, metal sulfides, and iron oxides. In all these chemically diverse minerals, the putative nanobacteria are approximately the same size, mainly 0.05 to 0.2 μm. This suggests a commonality of origin. At least for the type locality at Viterbo, Italy, the biogenicity of these minute cells has been supported by transmission electron microscopy (TEM). Slices through a green bioslime showed entities from 0.4 down to as small as 0.09 μm with definite cell walls and interior dots resembling ribosomes; and even smaller objects with cell walls and lucent interiors with diameters of 0.05 μm. Culturable organisms on earth are the same 0.05 μm size as the supposed nanobacteria on Mars.
- Prion — smallest known infectious agent (≈10 nm)
- Parvovirus — smallest known viruses (18-28 nm)
- Nanobe — possible smallest lifeforms (20 nm)
- Ultramicrobacteria — possible dormant forms of larger cells (200 nm)
- Mycoplasma — smallest known bacteria (300 nm)
- Nanoarchaeum — smallest known archaeum (400 nm)
- Pandoravirus — one of the largest known viruses (1000 nm)
- Pithovirus — largest known virus (1500 nm)
- Kajander E (2006). "Nanobacteria—propagating calcifying nanoparticles". Lett Appl Microbiol. 42 (6): 549–52. doi:10.1111/j.1472-765X.2006.01945.x. PMID 16706890.
- Ciftcioglu N, McKay D, Mathew G, Kajander E (2006). "Nanobacteria: fact or fiction? Characteristics, detection, and medical importance of novel self-replicating, calcifying nanoparticles". J Investig Med. 54 (7): 385–94. doi:10.2310/6650.2006.06018. PMID 17169260.
- Miller V, Rodgers G, Charlesworth J, Kirkland B, Severson S, Rasmussen T, Yagubyan M, Rodgers J, Cockerill F, Folk R, Rzewuska-Lech E, Kumar V, Farell-Baril G, Lieske J (2004). "Evidence of nanobacterial-like structures in calcified human arteries and cardiac valves". Am J Physiol Heart Circ Physiol. 287 (3): H1115–24. doi:10.1152/ajpheart.00075.2004. PMID 15142839.
- Cisar J, Xu D, Thompson J, Swaim W, Hu L, Kopecko D (2000). "An alternative interpretation of nanobacteria-induced biomineralization". Proc Natl Acad Sci USA. 97 (21): 11511–5. Bibcode:2000PNAS...9711511C. doi:10.1073/pnas.97.21.11511. PMC . PMID 11027350.
- Martel J, Young JD (April 2008). "Purported nanobacteria in human blood as calcium carbonate nanoparticles". Proc. Natl. Acad. Sci. U.S.A. 105 (14): 5549–54. Bibcode:2008PNAS..105.5549M. doi:10.1073/pnas.0711744105. PMC . PMID 18385376.
- Jack Maniloff, quoted in "The Rise and Fall of Nanobacteria", Young and Martel, Scientific American, January 2010
- Raoult, D; Drancourt, M; Azza, S; Nappez, C; Guieu, R; Rolain, JM; Fourquet, P; Campagna, B; et al. (2008). "Nanobacteria Are Mineralo Fetuin Complexes". PLoS Pathogens. 4 (2): e41. doi:10.1371/journal.ppat.0040041. PMC . PMID 18282102.
- "The Rise and Fall of Nanobacteria", Young and Martel, Scientific American, January 2010
- A convention has been adopted between researchers to name -or spell- the nanoparticles isolated from geological specimens as nannobacteria, and those from biological specimens as nanobacteria.
- Folk, Robert L. (March 4, 1997). "Nanobacteria: surely not figments, but what under heaven are they?". naturalSCIENCE. Retrieved 2008-12-20.
- McKay, David S.; et al. (1996). "Search for Past Life on Mars: Possible Relic Biogenic Activity in Martian Meteorite ALH84001". Science. 273 (5277): 924–930. Bibcode:1996Sci...273..924M. doi:10.1126/science.273.5277.924. PMID 8688069.
- Kajander E, Ciftçioglu N (1998). "Nanobacteria: An alternative mechanism for pathogenic intra- and extracellular calcification and stone formation". Proc Natl Acad Sci USA. 95 (14): 8274–9. Bibcode:1998PNAS...95.8274K. doi:10.1073/pnas.95.14.8274. PMC . PMID 9653177.
- Kumar V, Farell G, Yu S, et al. (November 2006). "Cell biology of pathologic renal calcification: contribution of crystal transcytosis, cell-mediated calcification, and nanoparticles". J. Investig. Med. 54 (7): 412–24. doi:10.2310/6650.2006.06021. PMID 17169263.
- Puskás L, Tiszlavicz L, Rázga Z, Torday L, Krenács T, Papp J (2005). "Detection of nanobacteria-like particles in human atherosclerotic plaques". Acta Biol Hung. 56 (3–4): 233–45. doi:10.1556/ABiol.56.2005.3-4.7. PMID 16196199.
- Ciftçioglu N, Haddad R, Golden D, Morrison D, McKay D (2005). "A potential cause for kidney stone formation during space flights: enhanced growth of nanobacteria in microgravity". Kidney Int. 67 (2): 483–91. doi:10.1111/j.1523-1755.2005.67105.x. PMID 15673296.
- Raoult D, Drancourt M, Azza S, et al. (February 2008). "Nanobacteria Are Mineralo Fetuin Complexes". PLoS Pathog. 4 (2): e41. doi:10.1371/journal.ppat.0040041. PMC . PMID 18282102.
- García-Ruiz JM, Melero-García E, Hyde ST (January 2009). "Morphogenesis of self-assembled nanocrystalline materials of barium carbonate and silica" (PDF). Science. 323 (5912): 362–5. Bibcode:2009Sci...323..362G. doi:10.1126/science.1165349. PMID 19150841.
- Folk, RL and Lynch. FL (2001) Organic matter, putative nanobacteria and the formation of oolites and hard grounds, Sedimentology, 48:215-229.
- Folk, RL and Lynch, FL, (1997) The possible role of nanobacteria (dwarf bacteria) in clay-mineral diagenesis, Journal of Sedimentary Research, 67:583-589.
- Folk, RL (2005) nanobacteria and the formation of framboidal pyrite, Journal Earth System Science, 114:369-374
- Folk, RL and Carlin J (2006) Adventures in an iron birdbath: nanostructure of iron oxide and the nanobacteria connection, Geological Society of America, Abstracts with programs, v. 38 (3), p. 6.
- Kirkland, B and Lynch, FL (2005) nanobacteria, Big Foot and the Loch Ness Monster—what are you supposed to believe?, Geological Society of America, abs. with progr., v. 37:253.
- Folk, RL and Kirkland, B, (2007) On the smallness of life: new TEM evidence from biofilm in hot springs, Viterbo, Italy, Geological Society of America, abs. with proper., v. 39 (6) 421.
- Folk, RL and Taylor, L (2002) nanobacterial alteration of pyroxenes in Martian meteorite ALH84001, Meteorology and Planetary Science, v. 37:1057-1070.
- Nanobacteria: Facts or Fancies?
- From Scum, Perhaps the Tiniest Form of Life, NY Times December 23, 2006
- Abstract: American Journal Physiology — Heart and Circulatory Physiology May 13, 2004
- Cisar JO, Xu DQ, Thompson J, Swaim W, Hu L, Kopecko DJ (October 2000). "An alternative interpretation of nanobacteria-induced biomineralization". Proc. Natl. Acad. Sci. U.S.A. 97 (21): 11511–5. Bibcode:2000PNAS...9711511C. doi:10.1073/pnas.97.21.11511. PMC . PMID 11027350.
- Are Nanobacteria Making Us Ill?, Wired News, Mar. 14, 2005
- Claim made for new form of life, BBC News, May 19, 2004
- Miller VM, Rodgers G, Charlesworth JA, et al. (September 2004). "Evidence of nanobacterial-like structures in calcified human arteries and cardiac valves". Am. J. Physiol. Heart Circ. Physiol. 287 (3): H1115–24. doi:10.1152/ajpheart.00075.2004. PMID 15142839.
- Infectious Microorganism Linked to Kidney Stones and other Diseases, February 2005
- Nannobacteria Research Page of the Department of Geosciences, Mississippi State University
- Kajander EO, Ciftçioglu N (July 1998). "Nanobacteria: An alternative mechanism for pathogenic intra- and extracellular calcification and stone formation". Proc. Natl. Acad. Sci. U.S.A. 95 (14): 8274–9. Bibcode:1998PNAS...95.8274K. doi:10.1073/pnas.95.14.8274. PMC . PMID 9653177.
- New Scientist article about nanobacteria
- The Calcium Bomb — The Nanobacteria Link to Heart Disease and Cancer
- Taylor, Michael (1999). Dark Life. New York, NY: Scribner. ISBN 0-684-84191-6.
- The Time Travelers Academy, a science fiction novel; it tells a story about the nanobacteria found in Martian meteorites.
- Nannobacteria Research associated with Robert Folk.
- The Rise and Fall of Nanobacteria; January 2010; Scientific American; by John D. Young; Jan Martel; 8 Pages.
-  Selected publications of Robert L. Folk on nanobacteria
- "First detailed microscopy evidence of 'nanobacteria' at the lower size limit of life". Kurzweil AI. March 9, 2015. Retrieved 10 March 2015.