RAD17

From Wikipedia, the free encyclopedia
Jump to: navigation, search
RAD17
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases RAD17, CCYC, HR24L, RAD17SP, RAD24, RAD17 checkpoint clamp loader component
External IDs MGI: 1333807 HomoloGene: 32117 GeneCards: RAD17
RNA expression pattern
PBB GE RAD17 210826 x at fs.png

PBB GE RAD17 207405 s at fs.png

PBB GE RAD17 211228 s at fs.png
More reference expression data
Orthologs
Species Human Mouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001044371
NM_001283011
NM_011233

RefSeq (protein)
Location (UCSC) Chr 5: 69.37 – 69.41 Mb Chr 13: 100.62 – 100.65 Mb
PubMed search [1] [2]
Wikidata
View/Edit Human View/Edit Mouse

Cell cycle checkpoint protein RAD17 is a protein that in humans is encoded by the RAD17 gene.[3][4]

Function[edit]

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.[5]

Interactions[edit]

RAD17 has been shown to interact with:

References[edit]

  1. ^ "Human PubMed Reference:". 
  2. ^ "Mouse PubMed Reference:". 
  3. ^ Bao S, Shen X, Shen K, Liu Y, Wang XF (March 1999). "The mammalian Rad24 homologous to yeast Saccharomyces cerevisiae Rad24 and Schizosaccharomyces pombe Rad17 is involved in DNA damage checkpoint". Cell Growth Differ. 9 (12): 961–7. PMID 9869296. 
  4. ^ Parker AE, Van de Weyer I, Laus MC, Verhasselt P, Luyten WH (August 1998). "Identification of a human homologue of the Schizosaccharomyces pombe rad17+ checkpoint gene". J Biol Chem. 273 (29): 18340–6. doi:10.1074/jbc.273.29.18340. PMID 9660800. 
  5. ^ "Entrez Gene: RAD17 RAD17 homolog (S. pombe)". 
  6. ^ a b c Bao S, Tibbetts RS, Brumbaugh KM, Fang Y, Richardson DA, Ali A, Chen SM, Abraham RT, Wang XF (June 2001). "ATR/ATM-mediated phosphorylation of human Rad17 is required for genotoxic stress responses". Nature. 411 (6840): 969–74. doi:10.1038/35082110. PMID 11418864. 
  7. ^ a b Kim ST, Lim DS, Canman CE, Kastan MB (Dec 1999). "Substrate specificities and identification of putative substrates of ATM kinase family members". J. Biol. Chem. 274 (53): 37538–43. doi:10.1074/jbc.274.53.37538. PMID 10608806. 
  8. ^ a b c Bermudez VP, Lindsey-Boltz LA, Cesare AJ, Maniwa Y, Griffith JD, Hurwitz J, Sancar A (February 2003). "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro". Proc. Natl. Acad. Sci. U.S.A. 100 (4): 1633–8. doi:10.1073/pnas.0437927100. PMC 149884Freely accessible. PMID 12578958. 
  9. ^ a b c Rauen M, Burtelow MA, Dufault VM, Karnitz LM (September 2000). "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9". J. Biol. Chem. 275 (38): 29767–71. doi:10.1074/jbc.M005782200. PMID 10884395. 
  10. ^ Chang MS, Sasaki H, Campbell MS, Kraeft SK, Sutherland R, Yang CY, Liu Y, Auclair D, Hao L, Sonoda H, Ferland LH, Chen LB (Dec 1999). "HRad17 colocalizes with NHP2L1 in the nucleolus and redistributes after UV irradiation". J. Biol. Chem. 274 (51): 36544–9. doi:10.1074/jbc.274.51.36544. PMID 10593953. 
  11. ^ Post SM, Tomkinson AE, Lee EY (October 2003). "The human checkpoint Rad protein Rad17 is chromatin-associated throughout the cell cycle, localizes to DNA replication sites, and interacts with DNA polymerase epsilon". Nucleic Acids Res. 31 (19): 5568–75. doi:10.1093/nar/gkg765. PMC 206465Freely accessible. PMID 14500819. 
  12. ^ Dufault VM, Oestreich AJ, Vroman BT, Karnitz LM (Dec 2003). "Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene". Genomics. 82 (6): 644–51. doi:10.1016/s0888-7543(03)00200-3. PMID 14611806. 
  13. ^ Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A (September 2001). "Purification and characterization of human DNA damage checkpoint Rad complexes". Proc. Natl. Acad. Sci. U.S.A. 98 (20): 11236–41. doi:10.1073/pnas.201373498. PMC 58713Freely accessible. PMID 11572977. 

Further reading[edit]