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Anopheles
Anopheles stephensi
Scientific classification Edit this classification
Domain: Eukaryota
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Diptera
Family: Culicidae
Subfamily: Anophelinae
Genus: Anopheles
Meigen 1818
Species

For a full description, see section Systematics and the main article: Taxonomy of Anopheles
Some important species are:

Anopheles range map

Anopheles /əˈnɒf[invalid input: 'ɨ']liːz/[1] (Greek anofelís: "useless"[2]) is a genus of mosquito first described and named by J. W. Meigen in 1818.[3] About 460 species are recognised; while over 100 can transmit human malaria, only 30–40 commonly transmit parasites of the genus Plasmodium, which cause malaria in humans in endemic areas. Anopheles gambiae is one of the best known, because of its predominant role in the transmission of the most dangerous malaria parasite species (to humans) – Plasmodium falciparum.

The name comes from the Greek αν, an, meaning "not", and όφελος, óphelos, meaning "profit", and translates to "useless".[1] Anopheles mosquitoes kill about 440,000 people each year because of malaria.

Some species of Anopheles also can serve as the vectors for canine heartworm Dirofilaria immitis, the filariasis-causing species Wuchereria bancrofti and Brugia malayi, and viruses such as one that causes O'nyong'nyong fever. An association of brain tumor incidence and malaria suggests the Anopheles might transmit a virus or other agent that could cause a brain tumor.[4]

Mosquitoes in other genera (Aedes, Culex, Culiseta, Haemagogus and Ochlerotatus) can also serve as vectors of disease agents, but not malaria.

Evolution

The ancestors of Drosophila and the mosquitoes diverged 260 million years ago.[5] The culicine and Anopheles clades of mosquitoes diverged between 120 million years ago and 150 million years ago.[5][6] The Old and New World Anopheles species subsequently diverged between 80 million years ago and 95 million years ago.[5][6] Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago.[7] The Anopheles gambiae and Anopheles funestus clades diverged between 80 million years ago and 36 million years ago. A molecular study of several genes in seven species has provided additional support for an expansion of this genus during the Cretaceous period.[8]

The Anopheles genome, at 230–284 million base pairs (Mbp), is comparable in size to that of Drosophila, but considerably smaller than those found in other culicine genomes (528 Mbp–1.9 Gbp). Like most culicine species, the genome is diploid with six chromosomes.

The only known fossils of this genus are those of Anopheles (Nyssorhynchus) dominicanus Zavortink & Poinar contained in Dominican amber from the Late Eocene (40.4 million years ago to 33.9 million years ago) and Anopheles rottensis Statz contained in German amber from the Late Oligocene (28.4 million years ago to 23 million years ago).

Systematics

Main article: Taxonomy of Anopheles

The genus Anopheles is a subfamily of Anophelinae with three genera: Anopheles Meigen (nearly worldwide distribution), Bironella Theobald (Australia only) and Chagasia Cruz (Neotropics). The taxonomy remains incompletely settled. Classification into species is based on morphological characteristics – wing spots, head anatomy, larval and pupal anatomy, chromosome structure, and more recently, on DNA sequences.

The genus has been subdivided into seven subgenera based primarily on the number and positions of specialized setae on the gonocoxites of the male genitalia. The system of subgenera originated with the work of Christophers, who in 1915 described three subgenera: Anopheles (widely distributed), Myzomyia (later renamed Cellia) (Old World) and Nyssorhynchus (Neotropical). Nyssorhynchus was first described as Lavernia by Theobald. Edwards in 1932 added the subgenus Stethomyia (Neotropical distribution). Kerteszia was also described by Edwards in 1932, but then recognised as a subgrouping of Nyssorhynchus. It was elevated to subgenus status by Komp in 1937, and it is also found in the Neotropics. Two additional subgenera have since been recognised: Baimaia (Southeast Asia only) by Harbach et al. in 2005 and Lophopodomyia (Neotropical) by Antunes in 1937.

Two main groupings within the genus Anopheles are used: one formed by the Cellia and Anopheles subgenera and a second by Kerteszia, Lophopodomyia and Nyssorhynchus. Subgenus Stethomyia is an outlier with respect to these two taxa. Within the second group, Kerteszia and Nyssorhynchus appear to be sister taxa.

The number of species currently recognised within the subgenera is given here in parentheses: Anopheles (206 species), Baimaia (1), Cellia (216), Kerteszia (12), Lophopodomyia (6), Nyssorhynchus (34) and Stethomyia (5).

Taxonomic units between subgenus and species are not currently recognised as official zoological names. In practice, a number of taxonomic levels have been introduced. The larger subgenera (Anopheles, Cellia and Nyssorhynchus) have been subdivided into sections and series which in turn have been divided into groups and subgroups. Below subgroup but above species level is the species complex. Taxonomic levels above species complex can be distinguished on morphological grounds. Species within a species complex are either morphologically identical or extremely similar and can only be reliably separated by microscopic examination of the chromosomes or DNA sequencing. The classification continues to be revised.

Subgenus Nyssorhynchus has been divided in three sections: Albimanus (19 species), Argyritarsis (11 species) and Myzorhynchella (4 species). The Argyritarsis section has been subdivided into Albitarsis and Argyritarsis groups.

The Anopheles group was divided by Edwards into four series: Anopheles (worldwide), Myzorhynchus (Palearctic, Oriental, Australasian and Afrotropical), Cycloleppteron (Neotropical) and Lophoscelomyia (Oriental); and two groups, Arribalzagia (Neotropical) and Christya (Afrotropical). Reid and Knight (1961) modified this classification and consequently subdivided the subgenus Anopheles into two sections, Angusticorn and Laticorn and six series. The Arribalzagia and Christya Groups were considered to be series. The Laticorn Section includes the Arribalzagia (24 species), Christya and Myzorhynchus series. The Angusticorn section includes members of the Anopheles, Cycloleppteron and Lophoscelomyia series.

All species known to carry human malaria lie within either the Myzorhynchus or the Anopheles series.

Life stages

Like all mosquitoes, anophelines go through four stages in their life cycles: egg, larva, pupa, and imago. The first three stages are aquatic and last 5–14 days, depending on the species and the ambient temperature. The adult stage is when the female Anopheles mosquito acts as malaria vector. The adult females can live up to a month (or more in captivity), but most probably do not live more than two weeks in nature.

Eggs

Adult females lay 50–200 eggs per oviposition. The eggs are quite small (about 0.5 × 0.2 mm). Eggs are laid singly and directly on water. They are unique in that they have floats on either side. Eggs are not resistant to drying and hatch within 2–3 days, although hatching may take up to 2–3 weeks in colder climates.

Larvae

Anopheles larva from southern Germany, about 8 mm long
Feeding position of an Anopheles larva (A), compared to that of a nonanopheline mosquito (B)

The mosquito larva has a well-developed head with mouth brushes used for feeding, a large thorax and a nine-segment abdomen. It has no legs. In contrast to other mosquitoes, the Anopheles larva lacks a respiratory siphon, so it positions itself so that its body is parallel to the surface of the water. In contrast, feeding larva of a nonanopheline mosquito species attaches itself to the water surface with its posterior siphon, with their body pointing downwards.

Larvae breathe through spiracles located on the eighth abdominal segment, so must come to the surface frequently. The larvae spend most of their time feeding on algae, bacteria, and other microorganisms in the surface microlayer. They dive below the surface only when disturbed. Larvae swim either by jerky movements of the entire body or through propulsion with the mouth brushes.

Larvae develop through four stages, or instars, after which they metamorphose into pupae. At the end of each instar, the larvae molt, shedding their exoskeletons, or skin, to allow for further growth. First-stage larvae are about 1 mm in length; fourth-stage larvae are normally 5–8 mm in length.

The process from egg-laying to emergence of the adult is temperature dependent, with a minimum time of seven days.

The larvae occur in a wide range of habitats, but most species prefer clean, unpolluted water. Larvae of Anopheles mosquitoes have been found in freshwater or saltwater marshes, mangrove swamps, rice fields, grassy ditches, the edges of streams and rivers, and small, temporary rain pools. Many species prefer habitats with vegetation. Others prefer habitats with none. Some breed in open, sun-lit pools, while others are found only in shaded breeding sites in forests. A few species breed in tree holes or the leaf axils of some plants.

Pupae

The pupa is comma-shaped when viewed from the side. The head and thorax are merged into a cephalothorax with the abdomen curving around underneath. As with the larvae, pupae must come to the surface frequently to breathe, which they do through a pair of respiratory trumpets on their cephalothoraces. After a few days as a pupa, the dorsal surface of the cephalothorax splits and the adult mosquito emerges. The pupal stage lasts around 2–3 days in temperate areas.

Adults

Resting positions of adult Anopheles (A, B), compared to a nonanopheline mosquito (C)

The duration from egg to adult varies considerably among species, and is strongly influenced by ambient temperature. Mosquitoes can develop from egg to adult in as little as five days, but it can take 10–14 days in tropical conditions.

Like all mosquitoes, adult Anopheles species have slender bodies with three sections: head, thorax and abdomen.

The head is specialized for acquiring sensory information and for feeding. It contains the eyes and a pair of long, many-segmented antennae. The antennae are important for detecting host odors, as well as odors of breeding sites where females lay eggs. The head also has an elongated, forward-projecting proboscis used for feeding, and two maxillary palps. These palps also carry the receptors for carbon dioxide, a major attractant for the location of the mosquito's host.

The thorax is specialized for locomotion. Three pairs of legs and a pair of wings are attached to the thorax.

The abdomen is specialized for food digestion and egg development. This segmented body part expands considerably when a female takes a blood meal. The blood is digested over time, serving as a source of protein for the production of eggs, which gradually fill the abdomen.

Anopheles mosquitoes can be distinguished from other mosquitoes by the palps, which are as long as the proboscis, and by the presence of discrete blocks of black and white scales on the wings. Adults can also be identified by their typical resting position: males and females rest with their abdomens sticking up in the air rather than parallel to the surface on which they are resting.

Adult mosquitoes usually mate within a few days after emerging from the pupal stage. In most species, the males form large swarms, usually around dusk, and the females fly into the swarms to mate.

Males live for about a week, feeding on nectar and other sources of sugar. Females will also feed on sugar sources for energy, but usually require a blood meal for the development of eggs. After obtaining a full blood meal, the female will rest for a few days while the blood is digested and eggs are developed. This process depends on the temperature, but usually takes 2–3 days in tropical conditions. Once the eggs are fully developed, the female lays them and resumes host-seeking.

The cycle repeats itself until the female dies. While females can live longer than a month in captivity, most do not live longer than one to two weeks in nature. Their lifespans depend on temperature, humidity, and their ability to successfully obtain a blood meal while avoiding host defenses.

In a study by the London School of Hygiene & Tropical Medicine researchers found that female mosquitoes carrying malaria parasites are significantly more attracted to human breath and odours than uninfected mosquitoes.[9] The research team infected laboratory-raised Anopheles gambiae mosquitoes with Plasmodium parasites, leaving a control group uninfected. Then tests were run on the two groups to record their attraction to human smells. Female mosquitoes are particularly drawn to foot odours, and one of the tests showed infected mosquitoes landing and biting a prospective host repeatedly. The team speculates that the parasite improves the mosquitoes' sense of smell.[10] It may also reduce its risk aversion.

Habitat

Although malaria is nowadays limited to tropical areas, most notoriously the regions of sub-Saharan Africa, many Anopheles species live in colder latitudes (see this map from the CDC). Indeed, malaria outbreaks have, in the past, occurred in colder climates, for example during the construction of the Rideau Canal in Canada during the 1820s.[11] Since then, the Plasmodium parasite (not the Anopheles mosquito) has been eliminated from first world countries.

The CDC warns, however, that "Anopheles that can transmit malaria are found not only in malaria-endemic areas, but also in areas where malaria has been eliminated. The latter areas are thus constantly at risk of reintroduction of the disease.

Susceptibility to become a vector of disease

Some species are poor vectors of malaria, as the parasites do not develop well (or at all) within them. There is also variation within species. In the laboratory, selection strains of A. gambiae that are refractory to infection by malaria parasites is possible. These refractory strains have an immune response that encapsulates and kills the parasites after they have invaded the mosquito's stomach wall. Scientists are studying the genetic mechanism for this response. Genetically modified mosquitoes refractory to malaria possibly could replace wild mosquitoes, thereby limiting or eliminating malaria transmission.

Malaria transmission and control

Understanding the biology and behavior of Anopheles mosquitoes can help understand how malaria is transmitted, and can aid in designing appropriate control strategies. Factors affecting a mosquito's facility to transmit malaria include its innate susceptibility to Plasmodium, its host choice and its longevity. Factors that should be taken into consideration when designing a control program include the susceptibility of malaria vectors to insecticides and the preferred feeding and resting location of adult mosquitoes.

On December 21, 2007, a study published in PLoS Pathogens found the hetrolytic C-type lectin CEL-III from Cucumaria echinata, a sea cucumber found in the Bay of Bengal, impaired the development of the malaria parasite when produced by transgenic A. stephensi.[12] This could potentially be used to control malaria by spreading genetically modified mosquitoes refractory to the parasites, although numerous scientific and ethical issues must be overcome before such a control strategy could be implemented.

Preferred sources for blood meals

One important behavioral factor is the degree to which an Anopheles species prefers to feed on humans (anthropophily) or animals such as cattle or birds. Anthropophilic Anopheles are more likely to transmit the malaria parasites from one person to another. Most Anopheles mosquitoes are not exclusively anthropophilic or zoophilic. However, the primary malaria vectors in Africa, A. gambiae and A. funestus, are strongly anthropophilic and, consequently, are two of the most efficient malaria vectors in the world.

Once ingested by a mosquito, malaria parasites must undergo development within the mosquito before they are infectious to humans. The time required for development in the mosquito (the extrinsic incubation period) ranges from 10–21 days, depending on the parasite species and the temperature. If a mosquito does not survive longer than the extrinsic incubation period, then she will not be able to transmit any malaria parasites.

It is not possible to measure directly the lifespans of mosquitoes in nature, but indirect estimates of daily survivorship have been made for several Anopheles species. Estimates of daily survivorship of A. gambiae in Tanzania ranged from 0.77 to 0.84, meaning at the end of one day, between 77% and 84% will have survived.[13]

Assuming this survivorship is constant through the adult life of a mosquito, less than 10% of female A. gambiae would survive longer than a 14-day extrinsic incubation period. If daily survivorship increased to 0.9, over 20% of mosquitoes would survive longer than the same period. Control measures that rely on insecticides (e.g. indoor residual spraying) may actually impact malaria transmission more through their effect on adult longevity than through their effect on the population of adult mosquitoes.

Patterns of feeding and resting

Most Anopheles mosquitoes are crepuscular (active at dusk or dawn) or nocturnal (active at night). Some feed indoors (endophagic), while others feed outdoors (exophagic). After feeding, some blood mosquitoes prefer to rest indoors (endophilic), while others prefer to rest outdoors (exophilic), though this can differ regionally based on local vector ecotype, and vector chromosomal makeup, as well as housing type and local microclimatic conditions. Biting by nocturnal, endophagic Anopheles mosquitoes can be markedly reduced through the use of insecticide-treated bed nets or through improved housing construction to prevent mosquito entry (e.g. window screens). Endophilic mosquitoes are readily controlled by indoor spraying of residual insecticides. In contrast, exophagic/exophilic vectors are best controlled through source reduction (destruction of the breeding sites).

Gut flora

Because transmission of disease by the mosquito requires ingestion of blood, the gut flora may have a bearing on the success of infection of the mosquito host. This aspect of disease transmission has not been investigated until recently.[14] The larval and pupal gut is largely colonised by photosynthetic cyanobacteria, while in the adult, Proteobacteria and Bacteroidetes predominate. Blood meals drastically reduce the diversity of organisms and favor enteric bacteria.

Insecticide resistance

Insecticide-based control measures (e.g. indoor spraying with insecticides, bed nets) are the principal ways to kill mosquitoes that bite indoors. However, after prolonged exposure to an insecticide over several generations, mosquito populations, like those of other insects, may evolve resistance, a capacity to survive contact with an insecticide. Since mosquitoes can have many generations per year, high levels of resistance can evolve very quickly. Resistance of mosquitoes to some insecticides has been documented with just within a few years after the insecticides were introduced. Over 125 mosquito species have documented resistance to one or more insecticides. The evolution of resistance to insecticides used for indoor residual spraying was a major impediment during the Global Malaria Eradication Campaign. Judicious use of insecticides for mosquito control can limit the evolution and spread of resistance. However, use of insecticides in agriculture has often been implicated as contributing to resistance in mosquito populations. Detection of evolving resistance in mosquito populations is possible, so control programs are well advised to conduct surveillance for this potential problem.

Eradication

With substantial numbers of malaria cases affecting people around the globe, in tropical and subtropical regions, especially in sub-Saharan Africa, where millions of children are killed by this infectious disease, eradication is back on the global health agenda.[15]

Although malaria has existed since old times, its eradication was possible in Europe, North America, the Caribbean and parts of Asia and southern Central America during the first regional elimination campaigns in the late 1940s. However, the same results were not achieved in sub-Saharan Africa.[15]

Though the World Health Organization adopted a formal policy on the control and eradication of the malaria parasite since 1955,[16] only recently, after the Gates Malaria Forum in October 2007, did key organizations start the debate on the pros and cons of redefining eradication as a goal to control malaria.

Clearly, the cost of preventing malaria is much less than treating the disease, in the long run. However, eradication of mosquitoes is not an easy task. For effective prevention of malaria, some conditions should be met, such as conducive conditions in the country, data collection about the disease, targeted technical approaches to the problem, very active and committed leadership, total governmental support, sufficient monetary resources, community involvement, and skilled technicians from different fields, as well as an adequate implementation.[17]

A wide range of strategies is needed to achieve malaria eradication, starting from simple steps to complicated strategies which may not be possible to enforce with the current tools.

Although mosquito control is an important component of malaria control strategy, elimination of malaria in an area does not require the elimination of all Anopheles mosquitoes. For instance, in North America and Europe, although the vector Anopheles mosquitoes are still present, the parasite has been eliminated. Some socioeconomic improvements (e.g., houses with screened windows, air conditioning), once combined with vector reduction efforts and effective treatment, lead to the elimination of malaria without the complete elimination of the vectors. Some important measures in mosquito control to be followed are: discourage egg-laying, prevent development of eggs into larvae and adults, kill the adult mosquitoes, do not allow adult mosquitoes into places of human dwelling, prevent mosquitoes from biting human beings and deny them blood meals.[18]

Research in this sense continues, and a study has suggested sterile mosquitoes might be the answer to malaria elimination. This research suggests using the sterile insect technique, in which sexually sterile male insects are released to wipe out a pest population, could be a solution to the problem of malaria in Africa. This technique brings hope, as female mosquitoes only mate once during their lifetimes, and in doing so with sterile male mosquitoes, the insect population would decrease.[19] This is another option to be considered by local and international authorities that may be combined with other methods and tools to achieve malaria eradication in sub-Saharan Africa.

Parasites

A number of parasites of this genus are known to exist, including microsporidia of the genera Amblyospora, Crepidulospora, Senoma and Parathelohania.[20]

Microsporidia infecting the aquatic stages of insects, a group that includes mosquitoes and black flies, and copepods appear to form a distinct clade from those infecting terrestrial insects and fish. Two distinct life cycles are found in this group. In the first type, the parasite is transmitted by the oral route and is relatively species nonspecific. In the second, while again the oral route is the usual route of infection, the parasite is ingested within an already infected intermediate host. Infection of the insect larval form is frequently tissue-specific, and commonly involves the fat body. Vertical (transovarial) transmission is also known to occur.

Few phylogenetic studies of these parasites have been done, and their the relationship to their mosquito hosts is still being determined. One study suggested Parathelohania is an early diverging genus within this group.[21]

The parasite Wolbachia bacteria have also been studied for use as control agents.[22]

See also

References

  1. ^ a b "Anopheles - Define Anopheles at Dictionary.com". Dictionary.com.
  2. ^ Angus Stevenson (19 August 2010). Oxford Dictionary of English. Oxford University Press. pp. 64–. ISBN 978-0-19-957112-3.
  3. ^ Meigen, J. W. (1818). Systematische Beschreibung der Bekannten Europäischen Zweiflügeligen Insekten Vol. 1. Forstmann, Aachen, 332 pp.
  4. ^ Steven Lehrer (2010). "Anopheles mosquito transmission of brain tumor" (PDF). Medical Hypotheses. 74 (1): 167–168. doi:10.1016/j.mehy.2009.07.005. PMID 19656635.
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  7. ^ Marinotti O, Cerqueira GC, de Almeida LG, Ferro MI, Loreto EL, Zaha A, Teixeira SM, Wespiser AR, Almeida E Silva A, Schlindwein AD, Pacheco AC, da Silva AL, Graveley BR, Walenz BP, de Araujo Lima B, Ribeiro CA, Nunes-Silva CG, de Carvalho CR, de Almeida Soares CM, de Menezes CB, Matiolli C, Caffrey D, Araújo DA, de Oliveira DM, Golenbock D, Grisard EC, Fantinatti-Garboggini F, de Carvalho FM, Barcellos FG, Prosdocimi F, May G, de Azevedo GM Junior, Guimarães GM, Goldman GH, Padilha IQ, Batista JD, Ferro JA, Ribeiro JM, Fietto JL, Dabbas KM, Cerdeira L, Agnez-Lima LF, Brocchi M, de Carvalho MO, Teixeira MD, de Mascena Diniz Maia M, Goldman MH, Cruz Schneider MP, Felipe MS, Hungria M, Nicolás MF, Pereira M, Montes MA, Cantão ME, Vincentz M, Rafael MS, Silverman N, Stoco PH, Souza RC, Vicentini R, Gazzinelli RT, Neves RD, Silva R, Astolfi-Filho S, Maciel TE, Urményi TP, Tadei WP, Camargo EP, de Vasconcelos AT. The genome of Anopheles darlingi, the main neotropical malaria vector. Nucleic Acids Res
  8. ^ Jyotsana Dixit, Hemlata Srivastava, Meenu Sharma, Manoj K. Das, O.P. Singh, K. Raghavendra, Nutan Nanda, Aditya P. Dash, D. N. Saksena & Aparup Das (2010). "Phylogenetic inference of Indian malaria vectors from multilocus DNA sequences". Infection, Genetics and Evolution. 10 (6): 755–763. doi:10.1016/j.meegid.2010.04.008. PMID 20435167.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  9. ^ "Science Daily". sciencedaily.com.
  10. ^ "Malaria bug may give mosquitoes a super sense of smell". New Scientist.
  11. ^ William N. T. Wylie (1983). "Poverty, Distress, and Disease: Labour and the Construction of the Rideau Canal, 1826–32". Labour/Le Travail. 11: 7–29. doi:10.2307/25140199. JSTOR 25140199.
  12. ^ Shigeto Yoshida, Yohei Shimada , Daisuke Kondoh, Yoshiaki Kouzuma, Anil K. Ghosh, Marcelo Jacobs-Lorena & Robert E. Sinden (2007). "Hemolytic C-type lectin CEL-III from sea cucumber expressed in transgenic mosquitoes impairs malaria parasite development". PLoS Pathogens. 3 (12): e192. doi:10.1371/journal.ppat.0030192. PMC 2151087. PMID 18159942.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
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  14. ^ Wang Y, Gilbreath TM 3rd, Kukutla P, Yan G, Xu J (2011). Leulier, François (ed.). "Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya". PLoS ONE. 6 (9): e24767. doi:10.1371/journal.pone.0024767. PMC 3177825. PMID 21957459.{{cite journal}}: CS1 maint: multiple names: authors list (link) CS1 maint: numeric names: authors list (link) CS1 maint: unflagged free DOI (link)
  15. ^ a b Marcel Tanner & Don de kSavigny (2008). "Malaria eradication back on the table". Bulletin of the World Health Organization. 86 (2): 82–83. doi:10.2471/BLT.07.050633.
  16. ^ "Malaria Eradication". Retrieved 2010-05-04.
  17. ^ "Mosquito Eradication". Archived from the original on 3 May 2010. Retrieved 2010-05-04. {{cite web}}: Unknown parameter |deadurl= ignored (|url-status= suggested) (help)
  18. ^ "Mosquito Control". Archived from the original on May 1, 2008. Retrieved 2010-05-04.
  19. ^ "Are sterile mosquitoes the answer to malaria elimination?". Archived from the original on 30 April 2010. Retrieved 2010-05-04. {{cite web}}: Unknown parameter |deadurl= ignored (|url-status= suggested) (help)
  20. ^ A. V. Simakova & T. F. Pankova (2008). "Ecology and epizootology of microsporidia in malarial mosquitoes (Diptera: Culicidae) from the south of western Siberia". Parazitologiia (in Russian). 42 (2): 139–150. PMID 18664069.
  21. ^ Michael D. Baker, Charles R. Vossbrinck, James J. Becnel & Theodore G. Andreadis (1998). "Phylogeny of Amblyospora (Microsporida: Amblyosporidae) and related genera based on small subunit ribosomal DNA data: a possible example of host parasite cospeciation" (PDF). Journal of Invertebrate Pathology. 71 (3): 199–206. doi:10.1006/jipa.1997.4725. PMID 9538024.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  22. ^ Mosquito Parasite Fights Infectious Disease : Discovery News. News.discovery.com (2009-10-01). Retrieved on 2012-07-14.
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