The music video begins with Tity Boi getting up from bed for breakfast. He gets dressed and rides in a Lamborghini Gallardo, in which he picks up Lil Wayne, handing him a duffle bag. They then head to a concert. Meanwhile, Dolla Boy sells some CDs to a boy in exchange for a duffle bag, then receives a text message to meet Tity Boi and Lil Wayne. At the end of the video, all three of them are seen performing together.
Miami-based rapper Lil' Brianna, released a freestyle over the song's instrumental for her 2007 mixtape, Princess of Miami. UK rapper Kano, released a freestyle over the song's instrumental for his MC No.1 mixtape. Other remixes include a Travis Barker remix and a Sean P remix, respectively. American rappers Rick Ross and Young Jeezy also have their own respective remixes.
The song was used by Chris Rock as the music he exited the stage to on his 2008 'No Apologies' tour. As well as his entrance theme song to his show "Kill the Messenger". Drake used Lil Wayne's lyrics of "Duffle Bag Boy" for his song "Underground Kings" from his second studio album Take Care