St Thomas the Apostle was a parish church in Knightrider Street in the City of London. In existence by the late twelfth century, it was destroyed in the Great Fire of London in 1666 and not rebuilt.
The patronage of the church belonged to the canons of St Paul's and it is mentioned in the register of the Dean and Chapter as early as 1181.John Stow implies that was rebuilt some time in the late fourteenth century, describing John Barnes, Lord Mayor in 1371 as "a great builder of S. Thomas Apostles parish church as appeareth by his armes there both in stone and glasse".
The parish was staunchly Royalist in the years leading up to the Civil War In 1642 the rector, named. Cooper, was sequestered and imprisoned in Leeds Castle for his loyalty to the king.
St Thomas' was, with the majority of the churches in the City, destroyed by the Great fire in 1666., Following the fire, a Rebuilding Act was passed and a committee set up under Sir Christopher Wren to decide which would be rebuilt. Fifty-one were chosen, but St Thomas the Apostle was not among them. Instead the parish was united with that of St Mary Aldermary. Part of the site was used for the creation of Queen Street, though a small portion of the churchyard survived.