Born Antonia Louisa Brico to a Dutch Catholic unmarried mother in Rotterdam, Netherlands, Brico was renamed Wilhelmina Wolthius by her foster parents. She and her foster parents migrated to the United States in 1908 and settled in California. On leaving Oakland Technical High School  in Oakland in 1919 she was already an accomplished pianist and had experience in conducting. At the University of California, Berkeley, Brico worked as an assistant to the director of the San Francisco Opera. Following her graduation in 1923 she studied piano under a variety of teachers, most notably under Zygmunt Stojowski.
In 1927, Brico entered the Berlin State Academy of Music and in 1929 graduated from its master class in conducting, the first American to do so. During that period she was also a pupil of Karl Muck, conductor of the Hamburg Philharmonic Orchestra, with whom she studied for a further three years after graduation. Following her debut as a professional conductor with the Berlin Philharmonic Orchestra in February 1930, Brico worked with the San Francisco Symphony and the Hamburg Philharmonic winning plaudits from critics and the public. Appearances as guest conductor of the Musicians' Symphony Orchestra in Detroit, Washington, D.C. and other sites soon followed. In 1934, she was appointed conductor of the newly founded Women's Symphony Orchestra which, in January 1939 (following the admission of men), became the Brico Symphony Orchestra.
In July 1938, Brico was the first woman to conduct the New York Philharmonic, and in 1939 conducted the Federal Orchestra in concerts at the New York World's Fair. During an extensive European tour, in which she appeared both as a pianist and a conductor, Brico was invited by Jean Sibelius to conduct the Helsinki Symphony Orchestra.
Brico settled in Denver, Colorado from 1942 onwards. Here she founded a Bach Society and the Women's String Ensemble. She also conducted the Denver Businessmen's Orchestra, which in 1968 became the Brico Symphony Orchestra, and in 1948 became conductor of the Denver Community Symphony (later the Denver Philharmonic). She was conductor of the Boulder Philharmonic Orchestra from 1958-1963. She taught piano to such students as Judy Collins and Donald Loach. Other notable piano and conducting students are James Erb and Karlos Moser. Brico continued to appear as guest conductor with orchestras around the world, including the Japan Women's Symphony.
A documentary film about Brico's life, entitled Antonia: A Portrait of a Woman, by director Jill Godmilow, with help from Brico's former student, Judy Collins, appeared in 1974. The film was partially responsible for invitations for Brico to conduct the Mostly Mozart Festival Orchestra in sold-out concerts recorded by Columbia Records in 1975, and the Brooklyn Philharmonia in 1977. Brico died in 1989 after a long illness at the age of 87. She had lived at the Bella Vita Towers, a nursing home in Denver since 1988. History Colorado, formerly the Colorado Historical Society, holds a large collection of her personal papers.
Dutch director Maria Peters wrote and directed a movie about the life of Brico, which starred Christanne de Bruijn as Antonia Brico. The movie, titled 'De Dirigent' ('The Conductor'), was released on October 25, 2018.
- Ware, Susan (2004-01-01). Notable American Women: A Biographical Dictionary Completing the Twentieth Century. Harvard University Press. pp. 77–79. ISBN 9780674014886.
- Macleod, Beth Abelson (2000). Women performing music: the emergence of American women as classical instrumentalists and conductors. Jefferson, NC [u.a.]: McFarland. pp. 124–39. ISBN 0786409045.
- Macleod, Beth Abelson (2000-12-01). Women Performing Music: The Emergence of American Women as Classical Instrumentalists and Conductors. McFarland. pp. 124–139. ISBN 9780786409044.
- Rowell, Margaret. "Master teacher of cellists, and humble student of nature : oral history transcript / and related material, 1982-1984". Archive.org. Retrieved 20 February 2016.
- "The Brico Requiem". Westword. Retrieved 20 February 2016.
- Wolthius, Wilhelmina. Oakland Technical High School Historical Archive http://oaklandtech.com/staff/centennial/2015/02/22/wilhelmina-wolthius-class-of-1919/. Retrieved 20 February 2016. Missing or empty
- "Antonia Brico". Encyclopædia Britannica. Retrieved August 29, 2013.
- "Denver Philharmonic Orchestra". denverphilharmonic.org.
- Kozinn, Allan (August 5, 1989). "Antonia Brico, 87, a Conductor; Fought Barriers to Women in 30's". New York Times.
- Blomster, Wes (October 5, 2007). "Musical milestone: Boulder Philharmonic celebrates 50 years". Daily Camera.
- Jane Weiner Lepage, "Women Composers, Conductors, and Musicians of the Twentieth Century", (Scarecrow Press, New Jersey, 1980). ISBN 9780810820821