|, CPL, hephaestin, Hephaestin|
|View/Edit Human||View/Edit Mouse|
Hephaestin is involved in the metabolism and homeostasis of iron and possibly copper. It is a transmembrane copper-dependent ferroxidase responsible for transporting dietary iron from intestinal enterocytes into the circulatory system. The highest expression of hephaestin is found in small intestine. It is limited to enterocytes of the villi (where the iron absorption takes place), being almost absent in crypt cells. Hephaestin converts iron(II) state, Fe2+, to iron(III) state, Fe3+, and mediates iron efflux most likely in cooperation with the basolateral iron transporter, ferroportin 1. To a lesser extent hephaestin has been detected in colon, spleen, kidney, breast, placenta and bone trabecular cells but its role in these tissues remains to be established. Hephaestin presents homology with ceruloplasmin, a serum dehydrogenase protein involved in copper detoxification and storage.
Much of what is known about hephaestin comes from studying heritable mutants of murine iron metabolism. The protein was discovered and identified through the study of mice with sex-linked anemia, or sla mice, in which there is normal mucosal uptake of dietary iron but impaired transport of iron from the intestinal enterocytes into the circulation. sla mice harbor a partial deletion mutation of the HEPH gene, resulting in the expression of a hephaestin protein that is truncated by 194 amino acids. Studies suggest that this truncated hephaestin protein still retains a minimal, yet detectable and quantifiable level of ferroxidase activity. This raises the possibility that alternative factors may contribute to the decreased efflux of iron seen in the sla phenotype.
In addition to the truncation of the original protein, the iron-deficient sla phenotype may also be explained by the intracellular mislocalization of hephaestin. Wild type hephaestin localizes in a supra nuclear compartment as well as the basolateral surface. In contrast, sla hephaestin seems to localize only in the supranucelar compartment but is largely undetectable in the latter. Given hephaestin’s established function in facilitating basolateral iron export, this mislocalization may explain the paradoxical intestinal iron accumulation and systemic iron deficiency observed in sla mice.
Human hephaestin, lacking the putative transmembrane domain, was first recombinantly expressed in 2005 by Drs. Tanya Griffiths, Grant Mauk, and Ross MacGillivray at the University of British Columbia. They demonstrated that recombinant human hephaestin (rhHp) bound copper (determined by inductively coupled plasma mass spectrometry) and exhibited an absorption maximum at ~610 nm consistent with other blue multicopper oxidases such as ceruloplasmin. By using ferrous ammonium sulfate as a substrate, rhHp was shown to have ferroxidase activity with a Km of 2.1 μM for Fe(II).
Hephaestin is a member of the family of copper oxidases that includes mammalian ceruloplasmin, yeast fet3 and fet5, and bacterial ascorbate oxidase, among others. While hephaestin shares 50% amino acid sequence identity with its serum homologue ceruloplasmin, the hephaestin protein includes an additional 86 amino acids at the C-terminus, which code for a single transmembrane domain and a short cytoplasmic tail. While the structure and kinetic activity of ceruloplasmin have been studied extensively, hephaestin has yet to be investigated at a similar level. Comparative models of hephaestin's structure have been created using established crystallographic data from ceruloplasmin, and these studies suggest that many of the structural features important in the enzymatic function of the latter are also conserved in the former. In particular, these shared features include cysteine residues involved in disulfide bond formation, histidine residues involved in copper binding, and residues involved in the binding of the iron substrate.
The regulation of hephaestin expression and the protein’s role in the larger picture of iron metabolism and homeostasis remain an active area of research. Some studies suggest mechanisms for local and systemic control of intestinal iron transport, in which high dietary iron intake and sufficient iron stores lead to down-regulation of DMT1, ferroportin (Ireg1) and hephaestin protein, thus minimizing iron absorption from the enterocytes into the circulation. Conversely, it is suggested that states of low dietary intake and low iron stores induce up-regulation of DMT1 as well as Ireg1 and hephaestin, thus simultaneously increasing the enterocyte’s capacity for dietary iron uptake on the basolateral surface and export into the circulation on the apical surface.
Relevance in Biology and Disease
Hephaestin has not yet been linked to a human disease. However, when the protein was ablated in murine models, both intestine-specific and whole-body hephaestin knockout (KO) strains exhibited similarly severe accumulation of iron in the duodenal enterocytes and suffered from microcytic, hypochromic anemia, indicative of systemic iron deficiency. The shared phenotype between the two strains suggests that intestinal hephaestin plays an important role in maintaining whole-body iron homeostasis. However, since both strains were viable, it is likely that hephaestin is not essential and other compensatory mechanisms exist to keep these mice alive.
In addition to the transport of iron from the intestine and into the circulation, ferroxidases also seem to play an important role in facilitating iron export from retinal cells. While deficiency in hephaestin or ceruloplasmin alone do not seem to cause iron buildup in the retina, studies done on murine models suggest that the combined deficiency is sufficient to cause age-dependent retinal pigment epithelium and retinal iron accumulation, with features consistent with macular degeneration. Hephaestin has been detected in mouse and human RPE (retinal pigment epithelial) cells as well as in rMC-1 cells (a rat Müller glial cell line), with greatest expression in the Müller endnote next to the internal limiting membrane.
- "Human PubMed Reference:".
- "Mouse PubMed Reference:".
- Ishikawa K, Nagase T, Suyama M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (June 1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 5 (3): 169–76. doi:10.1093/dnares/5.3.169. PMID 9734811.
- Vulpe CD, Kuo YM, Murphy TL, Cowley L, Askwith C, Libina N, Gitschier J, Anderson GJ (February 1999). "Hephaestin, a ceruloplasmin homologue implicated in intestinal iron transport, is defective in the sla mouse". Nat. Genet. 21 (2): 195–9. doi:10.1038/5979. PMID 9988272.
- "Entrez Gene: Hephaestin".
- Chen H, Huang G, Su T, Gao H, Attieh ZK, McKie AT, Anderson GJ, Vulpe CD (May 2006). "Decreased hephaestin activity in the intestine of copper-deficient mice causes systemic iron deficiency". J. Nutr. 136 (5): 1236–41. PMID 16614410.
- Chen H, Attieh ZK, Su T, Syed BA, Gao H, Alaeddine RM, Fox TC, Usta J, Naylor CE, Evans RW, McKie AT, Anderson GJ, Vulpe CD (May 2004). "Hephaestin is a ferroxidase that maintains partial activity in sex-linked anemia mice". Blood. 103 (10): 3933–9. doi:10.1182/blood-2003-09-3139. PMID 14751926.
- C. N. Roy; C. A. Enns (December 2000). "Iron homeostasis: new tales from the crypt". Blood. 96 (13): 4020–4027. PMID 11110669.
- Y. M. Kuo; T. Su; H. Chen; Z. Attieh; B. A. Syed; A. T. McKie; G. J. Anderson; J. Gitschier; C. D. Vulpe (February 2004). "Mislocalisation of hephaestin, a multicopper ferroxidase involved in basolateral intestinal iron transport, in the sex linked anaemia mouse". Gut. 53 (2): 201–206. doi:10.1136/gut.2003.019026. PMID 14724150.
- Griffiths TA, Mauk AG, MacGillivray RT (November 2005). "Recombinant expression and functional characterization of human hephaestin: a multicopper oxidase with ferroxidase activity". Biochemistry. 44 (45): 14725–31. doi:10.1021/bi051559k. PMID 16274220.
- Jiri Petrak; Daniel Vyoral (June 2005). "Hephaestin--a ferroxidase of cellular iron export". The international journal of biochemistry & cell biology. 37 (6): 1173–1178. doi:10.1016/j.biocel.2004.12.007. PMID 15778082.
- P. Bielli; L. Calabrese (September 2002). "Structure to function relationships in ceruloplasmin: a 'moonlighting' protein". Cellular and molecular life sciences : CMLS. 59 (9): 1413–1427. doi:10.1007/s00018-002-8519-2. PMID 12440766.
- Basharut A. Syed; Nick J. Beaumont; Alpesh Patel; Claire E. Naylor; Henry K. Bayele; Christopher L. Joannou; Peter S. N. Rowe; Robert W. Evans; S. Kaila S. Srai (March 2002). "Analysis of the human hephaestin gene and protein: comparative modelling of the N-terminus ecto-domain based upon ceruloplasmin". Protein engineering. 15 (3): 205–214. doi:10.1093/protein/15.3.205. PMID 11932491.
- Huijun Chen; Trent Su; Zouhair K. Attieh; Tama C. Fox; Andrew T. McKie; Gregory J. Anderson; Chris D. Vulpe (September 2003). "Systemic regulation of Hephaestin and Ireg1 revealed in studies of genetic and nutritional iron deficiency". Blood. 102 (5): 1893–1899. doi:10.1182/blood-2003-02-0347. PMID 12730111.
- Brie K. Fuqua; Yan Lu; Deepak Darshan; David M. Frazer; Sarah J. Wilkins; Natalie Wolkow; Austin G. Bell; JoAnn Hsu; Catherine C. Yu; Huijun Chen; Joshua L. Dunaief; Gregory J. Anderson; Chris D. Vulpe (2014). "The multicopper ferroxidase hephaestin enhances intestinal iron absorption in mice". PloS one. 9 (6): e98792. doi:10.1371/journal.pone.0098792. PMC . PMID 24896847.
- Paul Hahn; Ying Qian; Tzvete Dentchev; Lin Chen; John Beard; Zena Leah Harris; Joshua L. Dunaief (September 2004). "Disruption of ceruloplasmin and hephaestin in mice causes retinal iron overload and retinal degeneration with features of age-related macular degeneration". Proceedings of the National Academy of Sciences of the United States of America. 101 (38): 13850–13855. doi:10.1073/pnas.0405146101. PMID 15365174.
- Xining He; Paul Hahn; Jared Iacovelli; Robert Wong; Chih King; Robert Bhisitkul; Mina Massaro-Giordano; Joshua L. Dunaief (November 2007). "Iron homeostasis and toxicity in retinal degeneration". Progress in retinal and eye research. 26 (6): 649–673. doi:10.1016/j.preteyeres.2007.07.004. PMID 17921041.