Cholest-4-en-3-one 26-monooxygenase
| Cholest-4-en-3-one 26-monooxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.141 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Cholest-4-en-3-one 26-monooxygenase (EC 1.14.13.141, CYP125, CYP125A1, cholest-4-en-3-one 27-monooxygenase) is an enzyme with systematic name cholest-4-en-3-one,NADH:oxygen oxidoreductase (26-hydroxylating).[1][2][3][4] This enzyme catalyses the following chemical reaction
- cholest-4-en-3-one + NADH + H+ + O2 26-hydroxycholest-4-en-3-one + NAD+ + H2O
Cholest-4-en-3-one 26-monooxygenase is a heme thiolate (P450) enzyme.
References
- ^ Rosloniec, K.Z.; Wilbrink, M.H.; Capyk, J.K.; Mohn, W.W.; Ostendorf, M.; van der Geize, R.; Dijkhuizen, L.; Eltis, L.D. (2009). "Cytochrome P450 125 (CYP125) catalyses C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1". Mol. Microbiol. 74 (5): 1031–1043. doi:10.1111/j.1365-2958.2009.06915.x. PMID 19843222.
- ^ McLean, K.J.; Lafite, P.; Levy, C.; Cheesman, M.R.; Mast, N.; Pikuleva, I.A.; Leys, D.; Munro, A.W. (2009). "The Structure of Mycobacterium tuberculosis CYP125: molecular basis for cholesterol binding in a P450 needed for host infection". J. Biol. Chem. 284 (51): 35524–35533. doi:10.1074/jbc.M109.032706. PMC 2790982. PMID 19846552.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ Capyk, J.K.; Kalscheuer, R.; Stewart, G.R.; Liu, J.; Kwon, H.; Zhao, R.; Okamoto, S.; Jacobs, W.R.; Jr.; Eltis, L.D.; Mohn, W.W. (2009). "Mycobacterial cytochrome P450 125 (cyp125) catalyzes the terminal hydroxylation of C27 steroids". J. Biol. Chem. 284 (51): 35534–35542. doi:10.1074/jbc.M109.072132. PMC 2790983. PMID 19846551.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ Ouellet, H.; Guan, S.; Johnston, J.B.; Chow, E.D.; Kells, P.M.; Burlingame, A.L.; Cox, J.S.; Podust, L.M.; de Montellano, P.R. (2010). "Mycobacterium tuberculosis CYP125A1, a steroid C27 monooxygenase that detoxifies intracellularly generated cholest-4-en-3-one". Mol. Microbiol. 77 (3): 730–742. doi:10.1111/j.1365-2958.2010.07243.x. PMC 2909382. PMID 20545858.
External links
- Cholest-4-en-3-one+26-monooxygenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)