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Serum amyloid A-like 1, also known as SAAL1, Synoviocyte proliferation-associated in collagen-induced arthritis 1, and SPACIA1 is a protein in humans encoded by the SAAL1 gene.[1]


Gene

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SAAL1 is located at chromosome 11p15.1[1]

Locus

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The human SAAL1 gene is located at position 11p15.1 on the minus strand spanning from base pairs 18080292-18106082 (25,790 bases).[1] It has 12 exons and 11 introns and encodes a single isoform.[1][2]

Members of the serum amyloid-A family such as SAA1 reside in the same loci as SAAL1.[2]

SAAL1 Gene Neighborhood at loci 11p15.1[3]

Promoter

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The promoter region (GXP_169676) is predicted to span from basepairs 18105980-18107207 and extends into the first exon of SAAL1.[4] Predicted transcription factors include TATA binding factors, NF-κB, and KLF4, KLF5, and KLF6.[5]

Expression

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SAAL1 is ubiquitously expressed at moderate levels across all human tissues with highest expression in testes as determined by RNA-sequencing and microarray expression profiling.[6][7]

Transcript

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Predicted 5' UTR binding proteins of the human SAAL1 transcript include SRSF3 and FXR2.[8] Predicted 3' UTR binding proteins include SRSF5 and U2AF2.[8] All predicted proteins are involved in mRNA splicing, export, and translation.[9][10][11][12]

Protein Structure

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I-TASSER generated model of SAAL1 tertiary structure. The N-terminus is blue and C-terminus is red.[13][14][15]

General Properties

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The SAAL1 protein has a single known isoform consisting of 474 amino acids with a molecular weight of 53.5 kDa.[1] It is an acidic protein with an isoelectric point of 4.4.[16]

Composition

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SAAL1 is abundant in aspartic acid (7.8% by composition) and deficient in glycine (3.4% by composition)compared to other human proteins.[17] It also has 44 more aspartic acid and glutamic acid residues compared to lysine and arginine, indicating an overall negative charge.[18] Two negatively charged and glutamic acid abundant segments were identified and labeled in the SAAL1 conceptual translation.[17]

Domains and Motifs

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SAAL1 contains an armadillo-like fold with an enveloped fungal symportin-1 like region.[19][20] Other motifs were predicted by ELM[21] and MyHits Motif Scan.[22]

Predicted Motifs
Predicted Motifs Amino Acids Tools
Casein kinase 2 phosphorylation site 152-155, 165-168 MyHits[22], ELM[21]
Nuclear Export Signal 72-84 ELM[21]
MAPK docking site 106-115, 344-352 ELM[21]

Sub-Cellular Localization

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Immunofluorescent staining has identified SAAL1 localization in the nucleus of Caco-2 cells[23]. However, western blotting of hepatocellular carcinoma cell lines identified SAAL1 localization in the cytoplasm with minor amounts in the cell membrane and nucleus.[24]

Post-Translational Modifications

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SAAL1 mRNA sequence and translated amino acid sequence. Exon boundaries, start and stop codons, motifs, and post-translational modifications are annotated. Well conserved base pairs and amino acids are bolded

SAAL1 undergoes phosphorylation at two experimentally verified sites: Ser6 and Thr387.[20] Predicted post-translational modifications are detailed in the following table.

Predicted Post-Translational Modifications
Tool Predicted Modification Amino Acids
NetPhos[25][26] Casein kinase 2 phosphorylation Thr152, Ser165
YinOYang[27][28] O-linked glycosylation Ser6
SMART[29] Ubituitination Lys209, Val302

Clinical Significance

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SAAL1 overexpression has been correlated with the proliferation of rheumatoid and osteoarthritic synovial fibroblasts as well as disease progression.[19] [30]RNAi knockouts of SAAL1 reduced arrested fibroblasts in G0/G1 phase and reduced proliferation by 20% with a 50% reduction when fibroblasts were stimulated by TNF-α[19]. Stability assays reveal that SAAL1 promotes G1/S transition via CDK6 mRNA stabilization[19][30]. This finding was corroborated by SAAL1 knockdowns in hepatocellular carcinomas which also demonstrated impaired HGF-induced migration and increased sensitivity to sorafenib and foretinib treatment.[24] Additionally, SAAL1 is overexpressed in hepatocellular carcinoma cells and in chondrocytes stimulated by interleukin-1 beta, but this effect is diminished in the presence of glucosamine.[24][31]

Studies of the rock bream SAAL1 ortholog noted an increase in gene expression in response to bacterial and viral pathogens.[32] Human SAAL1 has been reported to interact with the M protein of SARS-Cov-2[33], Orf4 of Kaposi’s sarcoma-associated herpesvirus[34], and the M and M2 proteins of influenza A.[35] It has also been reported as an interferon stimulator [36]and TRIM25 interactor[37]. Other interacting proteins include PNKD[38] (which plays a role in cardiac hypertrophy via NF-κB signaling)[39], TMIGD3[40](which inhibits NF-κB activity)[41], and MARK3.[42]

Evolution

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Homology

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Three page PDF of multiple sequence alignment of vertebrate SAAL1 orthologs referenced in the orthologs table.[17][43]

BLAST searches have found homologs for SAAL1 in organisms as distant as plants, though few orthologs were found for fungi.[44] The following table provides a sample of the ortholog space. Vertebrate orthologs share >50% identity with human protein SAAL1 while displayed invertebrates and non-metazoan orthologs have 30% or less identity.

Sample of SAAL1 Orthologs
Species Organism Common Name Multiple Sequence Alignment Abbreviation Date of Divergence from Humans

(Millions of Years Ago)[45]

Length (AAs) Identity NCBI Accesion
Homo sapiens Humans Hsa_SAAL1 0 474 100 NP_612430.2
Macaca mulatta Rhesus Monkey Mmu_SAAL1 29 473 98 XP_001087433.2
Ictidomys tridecemlineatus Thirteen-Lined Ground Squirrel Itr_SAAL1 90 474 90 XP_005326805.1
Monodelphis domestica Gray Short-Tailed Opossum Mdo_SAAL1 159 475 73 XP_007497074.1
Ornithorhynchus anatinus Platypus Oan_SAAL1 177 486 71 XP_028915648.1
Calidris pugnax Ruff Cpu_SAAL1 312 472 70 XP_014815565.1
Rhinatrema bivittatum Two-Lined Caecilian Rbi_SAAL1 352 472 61 XP_029438391.1
Erpetoichthys calabaricus Reedfish Eca_SAAL1 435 484 50 XP_028650019.1
Callorhinchus milii Australian Ghost Shark Cmi_SAAL1 473 474 54 XP_007885592.1
Saccoglossus kowalevskii Acorn Worm Ski_SAAL1 684 508 28 XP_002732678.2
Pomacea canaliculata Golden Apple Snail Pca_SAAL1 797 563 30 XP_025086883.1
Orbicella faveolata Mountainous Star Coral Ofa_SAAL1 824 561 25 XP_020625180.1
Rhizopus microsporus (a fungal plant pathogen) Rmi_SAAL1 1105 323 14 XP_023467779.1
Phycomyces blakesleeanus (a type of fungus) Pbl_SAAL1 1105 346 14 XP_018285622.1
Manihot esculenta Cassava Mes_SAAL1 1496 536 20 XP_021611223.1
Lactuca sativa Lettuce Lsa_SAAL1 1496 534 19 XP_023753062.1
Lupinus angustifolius Narrowleaf Lupin Lan_SAAL1 1496 488 18 XP_019436310.1
Elaeis guineensis Oil Palm Egu_SAAL1 1496 568 18 XP_010933466.1
Phalaenopsis equestris (a type of orchid) Peq_SAAL1 1496 551 17 XP_020591929.1
Phoenix dactylifera Date Palm Pda_SAAL1 1496 508 17 XP_026661658.1

SAAL1 exists in up to four isoforms in other vertebrates. Across these orthologs, it is the only member of its gene family.

Two page PDF of multiple sequence alignment of invertebrate and non-metazoan SAAL1 orthologs in the table, excluding the fungi orthologs. Poorly aligned regions are not shown.[17][43]

A multiple sequence alignment of the vertebrate homologs demonstrated high conservation of the protein, especially in the armadillo-type fold and fungal symportin-1 like motif. An alignment of invertebrate and non-metazoan orthologs indicates drastic changes in the protein's primary structure, but some conservation in the labeled motifs. Highly similar amino acids were colored red and less similar amino acids were colored blue; "*" denotes conservation and "." denotes similarity.

Phylogeny

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The date of divergence from the human ortholog was compared to the corrected % divergence for SAAL1 orthologs. Compared against data for cytochrome c and fibrinogen alpha proteins in similar orthologs, SAAL1 evolved at a moderate rate.

SAAL1 evolutionary rate vs fibrinogen alpha and cytochrome c.

References

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