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DOTA or Dota may refer to:
- Dota (canton), a canton in the province of San José, Costa Rica
- The Declaration of the Americas on Diabetes (DOTA 1996). This is one of several regional declarations co-sponsored by the World Health Organization and the International Diabetes Federation, the first being the St. Vincent Declaration (WHO EURO 1989).
- DOTA (chelator), a chemical
- Defense of the Ancients, a custom scenario for the video game Warcraft III
- Dota (genre), multiplayer online battle arena sub-genre, sometimes simply called "Dota"
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