Influenza A virus subtype H7N9
Electron micrograph of Influenza A (H7N9).
|Date||March 31, 2013–present|
|Location|| China: Anhui, Jiangsu, Zhejiang, Hangzhou, Hunan, Jiangxi, Fujian, Beijing, Guangdong
|120 deaths (as of 17 March 2014)|
|394 cases (as of 17 March 2014)|
H7N9 is a bird flu strain of the species Influenza virus A (avian influenza virus or bird flu virus). Avian influenza A H7 viruses normally circulate amongst avian populations with some variants known to occasionally infect humans. An H7N9 virus was first reported to have infected humans in March 2013, in China. Cases continued to be reported throughout April and then dropped to only a few cases during the summer months. At the closing of the year, 144 cases had been reported of which 46 had died. It is known that influenza tends to strike during the winter months, and the second wave, which began in October, was fanned by a surge in poultry production timed for Chinese New Year feasts that began at the end of January. January 2014 brought a spike in reports of illness with 96 confirmed reports of disease and 19 deaths. As of April 11, 2014, the outbreak's overall total is 419, including 7 in Hong Kong, and the unofficial number of deaths is 127.
The World Health Organization (WHO) has identified H7N9 as "...an unusually dangerous virus for humans." Most of the cases resulted in severe respiratory illness, with a mortality rate of roughly 30 percent.  Researchers have commented on the unusual prevalence of older males among H7N9-infected patients. While several environmental, behavioral, and biological explanations for this pattern have been proposed, as yet, the reason is unknown.
It has been established that many of the human cases of H7N9 appear to have a link to live bird markets. As of January 2014, there has been no evidence of sustained human-to-human transmission, however a study group headed by one of the world’s leading experts on avian flu reported that several instances of human-to-human infection are suspected. H7N9 virus does not kill poultry, which makes surveillance much more difficult. Chinese scientists announced the development of a vaccine on October 26, 2013, but said that H7N9 had not spread far enough to merit widespread vaccination. Research regarding background and transmission is ongoing.
Influenza A viruses are divided into subtypes based on two proteins on the surface of the virus: hemagglutinin (HA) and neuraminidase (NA). The avian influenza A(H7N9) virus designation of H7N9 identifies it as having HA of the H7 subtype and NA of the N9 subtype.
Avian influenza A H7 viruses are a group of influenza viruses that normally circulate among birds. H7 influenza infections in humans are uncommon, but have been confirmed world-wide in people who have direct contact with infected birds. Most infections have been mild involving only conjunctivitis and mild upper respiratory symptoms. The avian influenza A(H7N9) virus is a subgroup among this larger group of H7 viruses. Although some H7 viruses (e.g. H7N2, H7N3 and H7N7) have occasionally been found to infect humans, H7N9 has previously been isolated only in birds, with outbreaks reported in the Netherlands, Japan, and the United States. Until the 2013 outbreak in China, no human infections with H7N9 viruses have ever been reported.
Genetic characterisation of avian influenza A(H7N9) shows that the H7N9 virus that infects human beings resulted from the recombination of genes between several parent viruses noted in poultry and wild birds in Asia. It is most closely related to sequences found in samples from ducks in Zhejiang province in 2011. Evidence so far suggests that the new H7N9 virus might have evolved from at least four origins. It is hypothesized that the gene that codes for HA has its origin in ducks and the gene that codes for NA has its origin with ducks and probably also wild birds. Six internal genes originated with at least two H9N2 chicken viruses. The HA genes were circulating in the East Asian flyway in both wild birds and ducks, while the NA genes were introduced from European lineages and transferred to ducks in China by wild birds through migration along the East Asian flyway.
Dr. Keiji Fukuda, WHO's assistant director-general for health security and environment, remarked at a Toronto interview that "I think we are genuinely in new territory here in which the situation of having something that is low path in birds (yet) appears to be so pathogenic in people... And then to have those genetic changes ... I simply don't know what that combination is going to lead to." "Almost everything you can imagine is possible. And then what's likely to happen are the things which you can't imagine," he also remarked.
According to the deputy director of CDC's influenza division, the genetic makeup of H7N9 is "disturbingly different" from that of the H5N1 virus that has infected more than 600 people over the past 10 years and killed more than half of them. "The thing that's different between them is the H5 virus still maintains a lot of the avian or bird flu characteristics, whereas this H7N9 shows some adaptation to mammals. And that's what makes it different and concerning for us. It still has a ways to go before it becomes like a human virus, but the fact is, it's somewhere in that middle ground between purely avian and purely human."
In August 2013, it was announced that scientists plan to create mutant forms of the virus so they can gauge the risk of it becoming a lethal human pandemic. The genetic modification work will result in highly transmissible and deadly forms of H7N9, and is being carried out in several high security laboratories around the world.
Reported cases in 2013
On March 31, 2013, the Centre for Health Protection (CHP) of the Department of Health of Hong Kong and the Chinese National Health and Family Planning Commission notified the World Health Organization of three confirmed human cases of influenza A (H7N9) in Shanghai and Anhui (illness onset between February 19 and March 15, 2013). On April 2, the CHP confirmed four more cases in Jiangsu province, all considered in critical condition in hospitals in Nanjing, Suzhou, and Wuxi. In a statement, the CHP said that no epidemiologic links had been found between the four patients and so far no other H7N9 infections have been identified in 167 of their close contacts.
The first reported death associated with H7N9 was an 87 year old man who died on March 4. A second man, aged 27, died on March 10. On April 3, Chinese authorities reported another death, bringing the number to three. On April 4, the number of reported cases was 14, with 5 deaths. The two victims were a 48 year old man and a 52 year old woman, both from Shanghai. On April 5, a farmer, aged 64, living in Huzhou (Zhejiang province), died, raising the death toll to 6. On April 6, the Chinese Ministry of Health reported 18 positive cases, death toll still at 6. Two days later, positive cases rose to 24 and one death case from Shanghai brought the death toll to 7.
On April 9, the Chinese National Health and Family Planning Commission announced "an additional three laboratory-confirmed cases of human infection with influenza A(H7N9) virus." The new patients "are two patients from Jiangsu – an 85-year-old man who became ill on 28 March 2013" and a "25-year-old pregnant woman who became ill on 30 March 2013" and "a 64-year-old man from Shanghai who became ill on 1 April 2013, and died on 7 April 2013". As of April 9, a "total of 24 cases have been laboratory confirmed with influenza A(H7N9) virus in China, including seven deaths, 14 severe cases and three mild cases." In Jiangsu, more than "600 close contacts of the confirmed cases are being closely monitored."
In an update on April 11, Xinhua reported 38 identified cases and 10 deaths. According to the WHO, of the 28 patients who had survived their infections, 19 illnesses were severe and 9 were mild. The WHO said they were monitoring 760 close contacts and so far had no evidence of ongoing human-to-human transmission.
On April 13, a seven year old girl from Beijing was the first confirmed case of H7N9 bird flu outside eastern China. On April 14, Xinhua Chinese state media reported two human cases in central Henan just west of the area where the disease had been centered. Totals included 61 infected and 13 dead. On April 14, Chinese officials also reported the first asymptomatic case in Beijing. A health department notice suggested that a 4-year-old boy had no clinical symptoms and was tested during surveillance of high-risk groups.
On April 17, a total of 82 cases had been confirmed, with 17 deaths. On April 18, China reported 87 confirmed cases. On April 20, there were 96 confirmed cases, of which 18 were fatal. The next day, confirmed cases rose to 102 and fatal cases to 20. On April 22, there were 104 cases with 21 deaths. On April 23, 3 more cases were reported in an update from the WHO. All of the newly reported cases were in older men from eastern China. Two cases came from the Zhejiang province and the third was from the Anhui province. Total cases count reached 108 with 22 deaths.
On April 25, the National Health and Family Planning Commission said that a total of 109 H7N9 cases had been reported within mainland China, including 23 deaths. However, Anne Kelso, director of the WHO Collaborating Centre for Reference and Research on Influenza, VIDRL, Australia, reported that researchers had seen a "dramatic slowdown" in human cases in Shanghai after the city's live poultry markets were closed on April 6. The following day cases in mainland China rose to 118.
On April 28, four provinces, Zhejiang, Shandong, Jiangxi and Fujian, reported new cases, raising the total number of cases in mainland China to 125 with 24 deaths. On May 2, there were 127 confirmed cases in mainland China, of which 27 were fatal, and including the case in Taiwan there were a total of 128 cases worldwide. On May 6, in a weekly update, China's Ministry of Health announced there were 129 confirmed cases in mainland China with 31 deaths, for a total of 130 cases worldwide.
On May 7, Hong Kong's Centre for Health Protection reported that there were 130 confirmed cases of H7N9 avian flu in mainland China following the hospitalization of a 79-year-old woman from China's Jiangxi province, bringing the count to 131 cases.
The Ministry of Health of People's Republic of China reported on July 10 that in the month of June, there was only 1 confirmed case, and there were a total of 132 confirmed cases in Mainland China as of June 30, 2013 (43 fatal, 85 patient recovery cases). Though there is a slow increase in the number of cases, China recently warned that the transmission of H7N9 virus might be active again by autumn and winter seasons.
In August, Guangdong province confirmed its first case of H7N9 bird flu, a 51-year-old woman in critical condition after having been admitted to a hospital on August 3.
As of November 1, 2013[update], China reported to the WHO that "rare and sporadic human infections with H7N9" have been reported with "the total number of cases reported to 137, including 45 deaths" in China. The CDC and U.S. government H7N9 preparedness efforts have continued over the summer and are "continuing to watch this situation closely".
On December 31, Taiwan’s CDC released a press statement indicating that an 86 year old man from Jiangsu Province, China, who was visiting Taiwan, became ill and tested positive for H7N9 flu. This is the second case in Taiwan, the first being in April.
Reported cases in 2014
On January 21, 2014, it was reported that a 31-year-old thoracic surgeon had died four days previously, the first medical professional to die from H7N9 flu. There was no evidence that he had been in contact with live poultry recently. Yuen Kwok-yung, a University of Hong Kong microbiologist, said, "If the diagnosis is confirmed and no [bird] exposure history is elicited, this does point slightly more to the possibility that H7N9 may be more transmissible between humans than H5N1".
On January 28 it was reported by the Chinese Center for Disease Control and Prevention that the virus had killed 20 people in China in 2014, with the total number of human infections at 102. That compares with 144 confirmed cases, including 46 deaths, in the whole of 2013. At the end of January more than half of 2014 cases had been in Zhejiang, with another 24 in Guangdong, and eight in Shanghai. The director of the Chinese National Influenza Center, Shu Yuelong, said a large-scale H7N9 epidemic still remains unlikely because the virus has not yet mutated in such a way that would allow person to person transmission.
Symptoms and treatment
According to the World Health Organization, symptoms include fever, cough, and shortness of breath, which may progress to severe pneumonia. The virus can also overload the immune system, causing what is known as a cytokine storm. Blood poisoning and organ failure are also possible. In an article in the The New England Journal of Medicine, doctors reported that most of the patients with confirmed cases of H7N9 virus infection were critically ill and that approximately 20% had died of acute respiratory distress syndrome (ARDS) or multiorgan failure.
Antigenic and genome sequencing suggests that H7N9 is sensitive to neuraminidase inhibitors, such as oseltamivir and zanamivir. The use of these neuraminidase inhibitors in cases of early infection may be effective, although the benefits of oseltamivir treatment have been questioned.
Information released in 2014 indicated that 75% of those that came down with H7N9 influenza had previously been exposed to domestic poultry. In April, 2013, the World Health Organization (WHO) said H7N9 was "unlikely" to become a pandemic and that there was no evidence of human-to-human transmission. In late July, 2013, however, Chinese scientists found evidence that person-to-person transmission was possible, but would not transmit easily.
In April 2013, it was also reported that the virus had been found only in chickens, ducks, and pigeons at live poultry markets and that no migratory birds had tested positive for the virus, easing concerns about that route of transmission. However, later investigation demonstrated that H7N9 may infect wild songbirds and caged parakeets which then shed the virus into their environment. This finding implies that these birds may potentially serve as intermediate hosts with the ability to facilitate transmission and dissemination of H7N9.
At an April 26 news conference, the WHO assistant director-general for health stated, "This is an unusually dangerous virus for humans. We think this virus is more easily transmitted from poultry to humans than H5N1." Furthermore, there is great concern because unlike the H5N1 viral form, H7N9 does not cause visible disease in poultry, which makes surveillance, prevention, and control of the virus in poultry extremely difficult.
On April 30, it was announced that the Ministry of Agriculture of the People's Republic of China had asked the Director General of the World Organisation for Animal Health (OIE) to send OIE experts to assess the situation and provide advice. According to the information and data collected, it was confirmed that many of the human cases of H7N9 appeared to have a link with live bird markets, but to that date no human cases or animal infections of H7N9 had been detected on poultry farms. The OIE experts made the hypothesis that people could be infected through exposure to infected birds in markets or to a contaminated environment such as live poultry markets where the virus is present. They believe that live bird markets may play a key role in human and animal infections with H7N9 and that, even if the overall level of infection is relatively low (having not been detected yet in poultry farms), live bird markets provide an environment for amplification and maintenance of the H7N9 virus.
The OIE mission also confirmed that currently infection with H7N9 does not cause visible disease in poultry and therefore veterinary services must be especially involved in preventing its further spread. According to the April 30 report, there is no evidence to suggest that the consumption of poultry or eggs could transmit the virus to humans. More assessment is needed to know whether poultry vaccination could be considered as a control option for H7N9. It will also be important to verify whether the H7N9 virus is transmissible from humans to animals because if established, it could be a potential channel for the global spread of the virus.
The number of cases detected after April fell abruptly. The decrease in the number of new human H7N9 cases may have resulted from containment measures taken by Chinese authorities, including closing live bird markets, or from a change in seasons, or a possibly a combination of both factors. Studies indicate that avian influenza viruses have a seasonal pattern, much like human seasonal influenza viruses. If this is the case, H7N9 infections – in birds and people – may pick up again when the weather turns cooler in China. Limited person-to-person spread of bird flu is thought to have occurred rarely in the past, most notably with avian influenza A (H5N1). According to the US CDC, based on previous experience, some limited human-to-human spread of this H7N9 virus would not be surprising if the virus reemerges in the fall. Furthermore, according to the WHO, since migratory birds were first implicated in H7N9 transmission, the possibility that the virus may spread into other regions or countries with colder weather cannot be excluded, given the widespread bird migratory patterns.
Human to human transfer of virus
In a study published in July, 2013, an international team led by Yoshihiro Kawaoka, one of the world’s leading experts on avian flu, reported that while avian flu viruses typically lack the ability to transfer through respiratory droplets, studies using ferrets, who like humans infect one another through coughing and sneezing, showed that one of the H7N9 strains isolated from humans can transmit through respiratory droplets. Kawaoka says, “H7N9 viruses combine several features of pandemic influenza viruses, that is their ability to bind to and replicate in human cells and the ability to transmit via respiratory droplets.” Further, because several instances of human-to-human infection are suspected, Kawaoka states that “If H7N9 viruses acquire the ability to transmit efficiently from person to person, a worldwide outbreak is almost certain since humans lack protective immune responses to these types of viruses.”
On August 6, 2013, the British Medical Journal released the results of epidemiological investigations conducted after a family cluster of two patients were infected with avian H7N9 in March 2013 and later died in April and May. A 60 year old man became infected after an exposure to poultry and his daughter, who had not been exposed to poultry but had cared for her ill father, became infected as well. Genome sequence and analyses of phylogenetic trees showed that both viruses were almost genetically identical. Forty-three close contacts of the infected patients did not become ill and they all tested negative for haemagglutination inhibition antibodies specific for avian H7N9. It was concluded that the infection of the daughter probably resulted from close contact with her father during unprotected exposure, suggesting that the virus was able to transmit from person to person. However, the researchers consider that the transmissibility of the virus remains limited and non-sustainable.
In the month following the report of the first case, more than 100 people had been infected, an unusually high rate for a new infection; a fifth of those patients had died, a fifth had recovered, and the rest remained critically ill. Keiji Fukuda, the World Health Organization's (WHO) assistant director-general for health, security and the environment, identified H7N9 as "...an unusually dangerous virus for humans." By early May the number of new cases sharply declined and the mortality rate remained at about 20%, however as seriously ill patients continued to die, the mortality rate rose to about 33% by July.
In early May it was reported that testing of more than 20,000 Chinese who had influenza-like illnesses in March and April turned up only six cases of H7N9 infection. "We did not find evidence of widespread mild disease, suggesting that the reported cases reflect the true distribution of infection and not a surveillance artifact," the researchers wrote. The study suggests that the reported mortality rate is accurate, however the researchers noted several limitations of the study, including that the testing was carried out in city hospitals only and therefore may not have detected cases in rural areas.
Researchers have commented on the unusual prevalence of older males among H7N9-infected patients. Two-thirds of persons who are ill from H7N9 are 50 years of age or older, which is an older age curve than that for H5N1. In addition, two-thirds of persons ill from H7N9 are male. Possible reasons for the prevalence of older males among H7N9-infected patients include: a difference in exposure between males and females due to gender-associated practices; biological differences between males and females; and the differences in healthcare-seeking behavior and healthcare access between males and females. Both the median age and male to female relationship appear to have remained stable: The February 2014, WHO report stated "...37 cases, the median age was 60 years, ranging from 5-84 years, with a male to female ratio of 3.6:1." 
Dr. Yuzo Arima and his colleagues at WHO report "While poultry exposure appears to be a common risk factor, the age distribution among reported cases also raises the question why so few young adults (i.e. those of working age exposed to poultry as vendors/LBM [live bird market] workers/breeders/transporters) have been reported. This not only suggests greater exposure among elderly men but also a possible greater biological susceptibility to more severe outcomes." Danuta M. Skowronski, MD, of the British Columbia Centre for Disease Control and three colleagues put forward the hypothesis that older Chinese men have more lifetime exposure to H7 avian flu viruses and thus have immune responses which are weakly cross-reactive but not protective. This immune phenomenon is called antibody-dependent enhancement (ADE), and is perhaps best known in cases of Dengue fever when a person who has previously been infected with one serotype of Dengue fever becomes infected many months or years later with a different serotype. It is thought to occur when weakly cross-reactive antibodies form bridging complexes to facilitate uptake and replication of related but non-identical variants.
Although China has been praised for its quick response, some experts believe that there would be great difficulty providing adequate supplies of a vaccine if the virus were to develop into a pandemic. According to an article in the Journal of the American Medical Association (JAMA) in May 2013, "Even with additional vaccine manufacturing capacity . . . the global public health community remains woefully underprepared for an effective vaccine response to a pandemic...There is no reason to believe that a yet-to-be-developed pandemic A(H7N9) vaccine will perform any better than existing seasonal vaccines or the A(H1N1)pdm09 vaccines [about 60% to 70% effectiveness], particularly with regard to vaccine efficacy in persons older than 65 years."
On October 26, 2013, Chinese scientists announced that they had successfully produced an H7N9 vaccine, the first influenza vaccine to be developed entirely in China. It was developed jointly by researchers from Zhejiang University, Hong Kong University, the Chinese Center for Disease Control and Prevention, China's National Institute for Food and Drug Control, and the Chinese Academy of Medical Sciences. Chinese National Influenza Center director Shu Yuelong said the vaccine passed tests on ferrets and had been approved for humans, but H7N9 has not spread far enough to merit widespread vaccination. The vaccine was developed from a throat swab of an infected patient taken April 3.
On November 12, 2013, US scientists at Novavax, Inc. announced the first successful clinical testing of an H7N9 vaccine in the New England Journal of Medicine. They had previously described the development, manufacture, and efficacy in mice of an A/Anhui/1/13 (H7N9) viruslike particle (VLP) vaccine produced in insect cells with the use of recombinant baculovirus. The vaccine combined the HA and neuraminidase (NA) of A/Anhui/1/13 with the matrix 1 protein (M1) of A/Indonesia/5/05. The study enrolled 284 adults (≥18 years of age) in a randomized, observer-blinded, placebo-controlled clinical trial of this vaccine.
The Centers for Disease Control and Prevention (CDC) began sequencing and development of a vaccine as routine procedure for any new transgenic virus. The CDC and vaccine manufacturers are developing a candidate virus to be used in vaccine manufacturing if there is widespread transmission. On September 18, 2013, NIH announced that researchers have begun testing an investigational H7N9 influenza vaccine in humans. Two Phase II trials are collecting data about the safety of the vaccine, immune system responses to different vaccine dosages, both with and without adjuvants. Healthy adults 19 to 64 years of age will be enrolled in the two studies. The inactivated vaccine was made with H7N9 virus that was isolated in Shanghai, China. Adjuvants are being tested with the vaccine to determine if an adequate immune response can be produced. In addition, during a pandemic, adjuvants may be used as part of a "dose-sparing strategy".
In response to a request from the CDC and Biomedical Advanced Research and Development Authority (BARDA), and after the H7N9 flu virus gene sequences were made available through the Global Initiative on Sharing All Influenza Data (GISAID), the J. Craig Venter Institute (JCVI), and Synthetic Genomics Vaccines, Inc. (SGVI) began working with Novartis to synthesize the genes of the new viral strain and supplied these synthesized genes to the CDC.
The scientific community has praised China for its transparency and rapid response to the outbreak of H7N9. In an editorial on April 24, 2013, the journal Nature said "China deserves credit for its rapid response to the outbreaks of H7N9 avian influenza, and its early openness in the reporting and sharing of data." This, in spite of initial worries by Chinese scientists and officials that they might lose credit for their work in isolating and sequencing the novel H7N9 virus, after learning that pharmaceutical company Novartis and the J. Craig Venter Institute had used their sequences to develop US-funded H7N9 vaccine without offering to collaborate with the Chinese team, according to Nature. They believed, the usage of their data was initially not handled in the spirit of the GISAID sharing mechanism, which requires scientists who use the sequences to credit and propose collaboration with those who deposited the data in GISAID. Nature cited a Chinese official who concluded that this situation was quickly mitigated once communication channels were opened and the parties agreed to collaborate, thanks to GISAID president Peter Bogner.
Despite concerns that vaccination of poultry against the H5N1 avian influenza virus over the last decade might have made it harder for Chinese veterinary technicians to spot the recent spread of the H7N9 virus, China’s Agriculture Ministry defended its policy of large-scale vaccination of poultry against the earlier bird flu strain, saying that it was not interfering with its efforts now to identify the emerging H7N9 virus.
On April 15, 2013, the RIWI Corporation, led by researcher Neil Seeman of the University of Toronto released data on 7,016 Chinese “fresh” (i.e. non-panel based) Internet users – with a 24.08% response rate – over 20 hours. The level of contagion awareness was 31% in Beijing, 38% in Hangzhou, 33% in Nanjing, 40% in Shanghai, 52% in Ürümqi, and 28% in Zhengzhou (Chi Square; P = 0.05). The result far exceeds that of other internet surveys, suggesting an intense relevancy of interest and sense of urgency related to the current disease outbreak in the minds of average Chinese citizens.
Efforts to prevent spread of disease
In April 2013, Shanghai's health ministry ordered culling of birds after pigeon samples collected at the Huhuai wholesale agricultural products market in Songjiang District of Shanghai showed H7N9 On April 4, 2013, Shanghai authorities closed a live-poultry-trading zone and began slaughtering all birds. Poultry trading areas in two other areas of the Minhang district were also closed. On April 6, 2013, all Shanghai live poultry markets closed temporarily in response to the H7N9 found in the pigeon samples. The same day, Hangzhou also closed its live poultry markets. After gene sequence analysis, the national avian flu reference laboratory concluded that the strain of the H7N9 virus found on pigeons was highly congenic with those found on persons infected with H7N9 virus, the ministry said. On April 22, 2013, Forbes quoted Chinese state media reporting $2.7 billion in poultry industry losses.
When January 2014 brought a dramatic increase in reports of disease, the Chinese government responded by halting live poultry trading in three cities in Zhejiang province where 49 cases and 12 deaths had been reported. In addition, live poultry trading in Shanghai was halted for three months. In Hong Kong, authorities reacted to the discovery of H7N9 in live chickens from the province of Guangdong by suspending imports of fresh poultry from mainland China for 21 days, culling 20,000 chickens, and other measures in an effort to control the spread of the virus. On February 18, it was announced that they would extend the ban for four months. The health minister also said that they plan to prevent diseased birds from entering the market by setting up a facility where imported poultry can be quarantined to ensure they are disease-free.
The WHO does not advise against travel to China at this point in time, as there is no evidence of human-to-human transmission of the virus.
- United States
On April 9, 2013, the Centers for Disease Control and Prevention (CDC) activated its Emergency Operations Center (EOC) in Atlanta at Level II, the second-highest level of alert. Activation was prompted because the novel H7N9 avian influenza virus has never been seen before in animals or humans and because reports from China have linked it to severe human disease. EOC activation will "ensure that internal connections are developed and maintained and that CDC staff are kept informed and up to date with regard to the changing situation."
On April 10, 2013, the Public Health Agency of Canada (PHAC) and the Canadian Food Inspection Agency (CFIA) spelled out bio-safety guidance for handling the H7N9 virus. They stated that work with live cultures must be conducted in biosafety level 3 (BSL-3) containment. They also said that studies growing H7N9 virus should not be done in labs that culture human influenza viruses and that personnel should not have contact with susceptible animals for 5 days after handling H7N9 samples.
All hospitals were informed to remain vigilant, and to notify Singapore's Ministry of Health (MOH) immediately of any suspected cases of avian influenza in individuals who have recently returned from affected areas in China. MOH advised returning travellers from affected areas in China (Shanghai, Anhui, Jiangsu, and Zhejiang) to look out for signs and symptoms of respiratory illness, such as fever and cough, and seek early medical attention if they are ill with such symptoms. MOH also advised individuals to inform their doctors of their travel history, should they develop these symptoms after returning to Singapore.
- "H7N9 Case Detected in Malaysia". CDC. 12 February 2014. Retrieved 18 February 2014. "Still no sustained human-to-human spread; risk assessment unchanged"
- "The fight against bird flu". Nature 496 (7446): 397. April 24, 2013. doi:10.1038/496397a. PMID 23627002. Retrieved 25 April 2013.
- "Hong Kong's first case of deadly H7N9 bird flu virus confirmed". SCMP. December 3, 2013.
- "Hong Kong sees second case of H7N9 bird flu in a week". SCMP. December 6, 2013.
- WHO: Global Alert and Response: Human infection with avian influenza A(H7N9) virus – update (accessed November 7, 2013)
- "H7N9 bird flu resurges in China ahead of Lunar New Year - CNN.com". CNN. January 30, 2014.
- "H7N9 Bird Flu Not Just a China Problem – TIME.com". Time.
- Study says Vietnam at H7N9 risk as two new cases noted | CIDRAP
- #H7N9 Human Cases 2014 | pandemicinformationnews.blogspot.com
- WPRO | Human Infection with Avian Influenza A(H7N9)
- Shadbolt, Peter (April 25, 2013). "WHO: H7N9 virus 'one of the most lethal so far'". CNN. Retrieved 25 April 2013.
- Li, Q.; Zhou, L.; Zhou, M.; Chen, Z.; Li, F.; Wu, H.; Xiang, N.; Chen, E. et al. (April 24, 2013). "Preliminary Report: Epidemiology of the Avian Influenza A (H7N9) Outbreak in China". New England Journal of Medicine 370 (6): 520–32. doi:10.1056/NEJMoa1304617. PMID 23614499. Archived from the original on May 2, 2013. Retrieved April 25, 2013.
- Gallagher, James (May 3, 2013). "Q&A: H7N9 bird flu". BBC News. Retrieved May 4, 2013.
- Arima, Y.; Zu, R.; Murhekar, M.; Vong, S.; Shimada, T. (2013). "Human infections with avian influenza A(H7N9) virus in China: preliminary assessments of the age and sex distribution". Western Pacific Surveillance and Response Journal (WHO) 4 (2). doi:10.5365/wpsar.2013.4.2.005.
- Skowronski, DM; Janjua, NZ; Kwindt, TL; De Serres, G (25 April 2013). "Virus-host interactions and the unusual age and sex distribution of human cases of influenza A(H7N9) in China, April 2013". Eurosurveillance (European Centre for Disease Prevention and Control) 18 (17). Retrieved 3 May 2013.
- Experts: Past exposures may help explain H7N9 age profile, Center for Infectious Disease Research & Policy, University of Minnesota, April 26, 2013. " . . The phenomenon of cross-reacting antibodies that facilitate infection is best known in dengue infections, according to Skowronski and colleagues. The dengue virus comes in four types, and a person who has a second dengue infection involving a different type from the first one can suffer a severe illness. . "
- "OIE expert mission finds live bird markets play a key role in poultry and human infections with influenza A(H7N9)". Paris: World Organisation for Animal Health. April 30, 2013. Retrieved May 2, 2013.
- Study puts troubling traits of H7N9 avian flu virus on display (July 10, 2013)
- "Chinese researchers develop H7N9 flu vaccine". Xinhua. 2013-10-26. Retrieved 7 November 2013.
- "Chinese develop vaccination for H7N9, bird flu". UPI. Oct 26, 2013. Retrieved 7 November 2013.
- "Frequently Asked Questions on human infection with influenza A(H7N9) virus, China". World Health Organization. 5 April 2013. Retrieved 9 April 2013.
- "Types of Influenza Viruses". CDC. March 22, 2012. Retrieved May 7, 2013.
- Schnirring, Lisa (April 1, 2013). "China reports three H7N9 infections, two fatal". CIDRAP News.
- Koopmans, M.; De Jong, M. D. (2013). "Avian influenza A H7N9 in Zhejiang, China". The Lancet 381 (9881): 1882–3. doi:10.1016/S0140-6736(13)60936-8. PMID 23628442.
- Schnirring, Lisa (May 2, 2013). "H7N9 gene tree study yields new clues on mixing, timing". CIDRAP News. Retrieved May 4, 2013.
- Liu, D.; Shi, W.; Shi, Y.; Wang, D.; Xiao, H.; Li, W.; Bi, Y.; Wu, Y. et al. (2013). "Origin and diversity of novel avian influenza A H7N9 viruses causing human infection: phylogenetic, structural, and coalescent analyses". The Lancet. doi:10.1016/S0140-6736(13)60938-1.
- Helen Branswell. "World's in 'new territory' with challenging new flu virus, WHO expert says". Times Colonist. Retrieved 2013-04-15.
- Officials Prepare For Another Flu Pandemic — Just In Case : Shots - Health News : NPR
- "Scientists to make mutant forms of new bird flu to assess risk". Reuters. August 7, 2013.
- "Notification of three human cases of H7N9 in Shanghai and Anhui". Info.gov.hk. 2013-03-31. Retrieved 2013-04-04.
- Schnirring, Lisa (April 2, 2013). "China reports 4 more H7N9 infections". CIDRAP News.
- Kaiman, Jonathan; Davison, Nicola (April 3, 2013). "China reports nine bird flu cases amid allegations of cover up on social media". London: The Guardian. Retrieved April 4, 2013.
- "Third death from H7N9 bird flu". London: The Guardian. April 4, 2013. Retrieved April 4, 2013.
- CNN Staff (April 4, 2013). "Report: China's bird flu death toll rises to 5". CNN. Retrieved April 5, 2013.
- Mullen, Jethro (April 5, 2013). "Chinese authorities kill 20K birds as avian flu toll rises to 6". CNN. Retrieved April 5, 2013.
- "4月6日人感染H7N9禽流感疫情信息" [April 6 - Human infection with the H7N9 avian flu] (in Chinese). Ministry of Health of the People's Republic of China. 2013-04-06. Retrieved 2013-04-15.
- Jacobs, Andrew (April 6, 2013). "China Escalates Its Response to Outbreak of Avian Flu". New York Times (Beijing). Retrieved May 3, 2013.
- "4月8日人感染H7N9禽流感疫情信息" [April 8 - Human infection with the H7N9 avian flu] (in Chinese). Ministry of Health of the People's Republic of China. 2013-04-08. Retrieved 2013-04-15.
- "Human infection with influenza A(H7N9) virus in China - update". World Health Organization. 9 April 2013. Retrieved 9 April 2013.
- An (11 April 2013). "Shanghai confirms 3 new H7N9 bird flu cases, including one death". Xinhua. Retrieved 11 April 2013.
- Schnirring, Lisa (April 11, 2013). "China reports five more H7N9 cases, one fatal". CIDRAP News.
- Schnirring, Lisa (April 13, 2013). "Beijing reports first H7N9 infection". CIDRAP News.
- "4月14日人感染H7N9禽流感疫情信息" [April 14 - Human infection with the H7N9 avian flu] (in Chinese). Ministry of Health of the People's Republic of China. 14 April 2013. Retrieved 15 April 2013.
- "China H7N9 bird flu spreads to new provinceâ state media". newsinfo.inquirer.net. Retrieved 2013-04-14.
- Schnirring, Lisa (April 14, 2013). "H7N9 spreads to central China as asymptomatic case reported". CIDRAP News.
- "4月17日人感染H7N9禽流感疫情信息" [April 17 - Human infection with the H7N9 avian flu] (in Chinese). Ministry of Health of the People's Republic of China. April 17, 2013. Retrieved May 7, 2013.
- "4月18日人感染H7N9禽流感疫情信息" [April 18 - Human infection with the H7N9 avian flu] (in Chinese). Ministry of Health of the People's Republic of China. April 17, 2013. Retrieved May 7, 2013.
- "中国人感染H7N9禽流感确诊96例" [96 confirmed cases of Chinese infected with H7N9 avian influenza] (in Chinese). Xinhua. April 20, 2013. Retrieved April 21, 2013.
- "4月21日人感染H7N9禽流感疫情信息" [April 21 - Human infection with the H7N9 avian flu] (in Chinese). Ministry of Health of the People's Republic of China. April 21, 2013. Retrieved April 22, 2013.
- "Bird flu scare spreads in China". CNN. April 22, 2013. Retrieved 23 April 2013.
- Flannery, Russell (April 22, 2013). "H7N9 Bird Flu Cases In China Rise To 104; Deaths At 21". Forbes. Retrieved 23 April 2013.
- Schnirring, Lisa (April 23, 2013). "China reports three more H7N9 infections, another death". CIDRAP News.
- Chen, Yi-Hua (April 24, 2013). "燒到台灣！H7N9首例53歲台商病重" [Taiwan infected! 53-year old man, Taiwan's first case of H7N9, is seriously ill.]. China Times (in Chinese). Retrieved May 5, 2013.
- Jim, Claire (April 24, 2013). "Taiwan man contracts H7N9 bird flu, first outside mainland China". Taipei. Reuters. Archived from the original on April 24, 2013. Retrieved April 24, 2013.
- "Seven more human cases of avian influenza A (H7N9) verified by NHFPC". Info.gov.hk. 2013-04-26. Retrieved 2013-04-26.
- Flannery, Russell (2013-04-28). "China H7N9 Bird Flu Cases Rise By Five; Four Provinces Report Illnesses In One Day". Forbes. Retrieved 2013-04-29.
- "Human infection with avian influenza A(H7N9) virus – update". WHO. April 29, 2013. Retrieved April 29, 2013.
- "19 H7N9 cases reported on Chinese mainland last week". Beijing: Xinhua. May 2, 2013. Retrieved May 2, 2013.
- Schnirring, Lisa (May 2, 2013). "Three deaths lift China's H7N9 fatality count to 27". CIDRAP News.
- "Human infection with avian influenza A(H7N9) virus – update". WHO. May 2, 2013. Retrieved May 2, 2013.
- "5月1日-5月6日人感染H7N9禽流感疫情信息" [May 1–May 6: Human infection with the H7N9 avian flu] (in Chinese). Ministry of Health of the People's Republic of China. May 6, 2013. Retrieved May 7, 2013.
- Schnirring, Lisa (May 6, 2013). "H7N9 toll grows to 130 cases, 31 deaths". CIDRAP News. Retrieved May 7, 2013.
- "1 Mainland H7N9 case reported". Hong Kong: Centre for Health Protection. May 7, 2013. Retrieved May 8, 2013.
- Lisa, Schnirring (May 7, 2013). "Chinese woman's illness lifts H7N9 case total to 131". CIDRAP News. Retrieved May 8, 2013.
- 2013年6月人感染H7N9禽流感疫情概况 - 中华人民共和国国家卫生和计划生育委员会
- H7N9最新消息：国家流感中心主任称H7N9今年秋冬会卷土重来 它不会自动消失-观察者网
- WHO | Human infection with avian influenza A(H7N9) virus – update
- Chen, Te-ping (Aug 10, 2013). "China's Guangdong Province Confirms Bird-Flu Case". Wall Street Journal.
- "H7N9 Update - October 28, 2013". Centers for Disease Control and Prevention. October 28, 2013. Retrieved 1 November 2013.
- Hong Kong reports first H7N9 death; officials step up border checks
- Taiwan - Man, 86, hospitalized H7N9 case - from Changzhou City, Jiangsu Province - China - December 31, 2013 - died - FluTrackers
- 20 bird flu death but epidemic deemed 'unlikely'- China.org.cn
- H5N1: Hong Kong: CHP has details on Malaysia H7N9 case
- Lu, Shuihua; Zheng, Yufang; Li, Tao; Hu, Yunwen; Liu, Xinian; Xi, Xiuhong et al. (July 2013). "Clinical Findings for Early Human Cases of Influenza A(H7N9) Virus Infection, Shanghai, China (ahead of print)". Emerging Infectious Diseases (CDC) 19 (7). doi:10.3201/eid1907.130612. Retrieved 4 May 2013.
- Emerg Infect Dis. Possible Role of Songbirds and Parakeets in Transmission of Influenza A(H7N9) Virus to Humans - FluTrackers
- "Chinese bird flu mutates". 3 News NZ. April 4, 2013.
- "Human infection with influenza A(H7N9) in China – update". WHO. Retrieved April 4, 2013.
- "Evidence suggests new bird flu spread among people", USAToday, August 7, 2013
- Avian Influenza A H7N9 - CDC
- The Outbreak of Avian Influenza A (H7N9) in China: Current Status and Future Prospects Lu L, He B, Jiang S - Biomed J
- Probable person to person transmission of novel avian influenza A (H7N9) virus in Eastern China, 2013: epidemiological investigation | BMJ
- CIDRAP >> Chinese study finds very few mild H7N9 cases
- Human infections with avian influenza A(H7N9) virus in China: preliminary assessments of the age and sex distribution
- CIDRAP >> Experts offer dim view of potential vaccine response to H7N9
- "New England Journal of Medicine Publishes Positive Data From Clinical Trial of Novavax’ Vaccine Against H7N9 Avian Flu" (November 13, 2013) Novavax press release
- CDC.GOV H7N9 Portal
- Roos, Robert (April 5, 2013). "CDC working on vaccine, tests for novel H7N9 virus". CIDRAP News.
- "Prepared Statement from J. Craig Venter, Ph.D., and the J. Craig Venter Institute and Synthetic Genomics Vaccines, Inc. on the H7N9 avian flu strain in China". J. Craig Venter Institute. 9 April 2013. Retrieved 9 April 2013.
- "China praised for transparency during bird flu outbreak". CBS News. April 11, 2013. Retrieved May 7, 2013.
- Schnirring, Lisa (1 May 2013). "Second case from Hunan raises H7N9 total to 128". Center for Infectious Disease Research & Policy. Retrieved 14 May 2013.
- Declan Butler and David Cyranoski (2 May 2013). "Flu papers spark row over credit for data". Nature Magazine 497 (7447): 14–15. doi:10.1038/497014a. Retrieved 14 May 2013.
- Press release no. 108 (16 April 2013). "Transmissibility of novel avian influenza: GISAID database provides essential data for control strategies". Federal Ministry of Food, Agriculture and Consumer Protection. Retrieved 14 May 2013.
- Bradsher, Kieth (April 12, 2013). "China Defends Vaccination of Poultry as Flu Spreads". New York Times (Hong Kong). Retrieved April 30, 2013.
- "New H7N9 Data: An Epidemic Rising?". RIWI. 2013-04-15. Retrieved 2013-04-19.
- Fang Yang (5 April 2013). "Shanghai begins culling poultry; one contact shows flu symptoms". english.news.cn. Retrieved 15 April 2013.
- Schnirring, Lisa (April 4, 2013). "China reports more H7N9 cases, deaths; virus may be in pigeons". CIDRAP News.
- Mungin, Lateef (April 6, 2013). "China closes poultry sale in second city after bird flu outbreak". CNN. Retrieved April 6, 2013.
- H7N9 bird flu: Chinese provinces halt live poultry trade | World news | theguardian.com
- Pandemic Information News
- "Ministry of Health closely monitoring the influenza A (H7N9) situation". Singapore Ministry of Health. 6 April 2013. Retrieved 9 April 2013.
- China's new bird flu sickens 38, kills 10 April 12, 2013 USA Today
- Roos, Robert (April 9, 2013). "CDC activates emergency center over H7N9". CIDRAP News.
- Public Health Agency of Canada. "Joint Biosafety Advisory - Influenza A(H7N9) virus". Retrieved April 10, 2013.
- Schnirring, Lisa (April 15, 2013). "China reports 3 new H7N9 cases, 64 total, 14 deaths". CIDRAP News.
- "Malaysia Bans Chicken Imports From China". Wall Street Journal. April 26, 2013. Retrieved April 30, 2013.
- "Vietnam bans China poultry after new bird flu strain deaths". Brunei Times. Retrieved April 4, 2013.