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M line may refer to:
- M line (mittel line or middle line), a structure in muscle; see Myofibril or Sarcomere
- M Ocean View, a light rail and former streetcar line in San Francisco, California
- Ashmont–Mattapan High Speed Line, a streetcar line in Boston and Milton, Massachusetts known as the M Line
- McKinney Avenue Transit Authority, a streetcar line in Dallas, Texas also named the M-Line
- Geometrisches Mittel, or Geometric mean
| disambiguation page lists articles associated with the title M line.
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