Nitric-oxide synthase (NAD(P)H-dependent)
| Nitric-oxide synthase (NAD(P)H-dependent) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.13.165 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
Nitric-oxide synthase (NAD(P)H-dependent) (EC 1.14.13.165, nitric oxide synthetase, NO synthase) is an enzyme with systematic name L-arginine,NAD(P)H:oxygen oxidoreductase (nitric-oxide-forming).[1][2][3] This enzyme catalyses the following chemical reaction
- 2 L-arginine + 3 NAD(P)H + 3 H+ + 4 O2 2 L-citrulline + 2 nitric oxide + 3 NAD(P)+ + 4 H2O (overall reaction)
- (1a) 2 L-arginine + 2 NAD(P)H + 2 H+ + 2 O2 2 N-omega-hydroxy-L-arginine + 2 NAD(P)+ + 2 H2O
- (1b) 2 N-omega-hydroxy-L-arginine + NAD(P)H + H+ + 2 O2 2 L-citrulline + 2 nitric oxide + NAD(P)+ + 2 H2O
Nitric-oxide synthase (NAD(P)H-dependent) binds heme (iron protoporphyrin IX) and tetrahydrobiopterin.
See also
References
- ^ Wang, Z.Q.; Lawson, R.J.; Buddha, M.R.; Wei, C.C.; Crane, B.R.; Munro, A.W.; Stuehr, D.J. (2007). "Bacterial flavodoxins support nitric oxide production by Bacillus subtilis nitric-oxide synthase". J. Biol. Chem. 282 (4): 2196–2202. doi:10.1074/jbc.M608206200. PMID 17127770.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ Gusarov, I.; Starodubtseva, M.; Wang, Z.Q.; McQuade, L.; Lippard, S.J.; Stuehr, D.J.; Nudler, E. (2008). "Bacterial nitric-oxide synthases operate without a dedicated redox partner". J. Biol. Chem. 283: 13140–13147. doi:10.1074/jbc.M710178200. PMC 2442334. PMID 18316370.
{{cite journal}}: CS1 maint: unflagged free DOI (link) - ^ Agapie, T.; Suseno, S.; Woodward, J.J.; Stoll, S.; Britt, R.D.; Marletta, M.A. (2009). "NO formation by a catalytically self-sufficient bacterial nitric oxide synthase from Sorangium cellulosum". Proc. Natl. Acad. Sci. USA. 106 (38): 16221–16226. doi:10.1073/pnas.0908443106. PMC 2752531. PMID 19805284.
External links
- Nitric-oxide+synthase+(NAD(P)H-dependent) at the U.S. National Library of Medicine Medical Subject Headings (MeSH)