The former Dutch film academy was squatted in 1999 by a group of artists. After being a breeding ground for a number of years, the building was bought by the user association Eerste Hulp Bij Kunst (EHBK) from the council in 2006. It is used as a multi-media alternative cultural centre, comparable to projects in other Dutch cities such as Extrapool in Nijmegen and WORM in Rotterdam.
OT301 is a venue for music and films, a non-profit print shop, artists workspace and an 'organic cultural kitchen' (a vegan restaurant) called De Peper. Formerly, it housed the offices of European Youth For Action and Green Pepper magazine. OT301 received the Amsterdamprijs voor de Kunsten (Amsterdam Prize for the Arts) from the Amsterdam Fonds voor de Kunst on 23 August 2007 from Amsterdam Mayor Job Cohen.
- Eerste Hulp Bij Kunst koopt OT301. Retrieved 16 Februari 2016
- Lohman, Kirsty (2015). PhD: Punk Lives: Contesting Boundaries in the Dutch Punk Scene (phd). Warwick University. Retrieved 8 April 2019.
- "OT301, CLARON MC FADDEN, AZIZ DISTINCTIVE WITH AMSTERDAM PRICE FOR THE ARTS". Wayback Machine. Archived from the original on 3 September 2011. Retrieved 19 April 2019.