Fish reproduction

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Typical egg of a bony fish, about 1 mm in diameter. Fish eggs are often released into the water column, where they drift as zooplankton

Fish reproductive organs include testes and ovaries. In most species, gonads are paired organs of similar size, which can be partially or totally fused.[1] There may also be a range of secondary organs that increase reproductive fitness. The genital papilla is a small, fleshy tube behind the anus in some fishes, from which the sperm or eggs are released; the sex of a fish often can be determined by the shape of its papilla.

Anatomy[edit]

Testicles[edit]

Most male fish have two testes of similar size. In the case of sharks, the testes on the right side is usually larger. The primitive jawless fish have only a single testis, located in the midline of the body, although even this forms from the fusion of paired structures in the embryo.[2]

Under a tough membranous shell, the tunica albuginea, the testis of some teleost fish, contains very fine coiled tubes called seminiferous tubules. The tubules are lined with a layer of cells (germ cells) that from puberty into old age, develop into sperm cells (also known as spermatozoa or male gametes). The developing sperm travel through the seminiferous tubules to the rete testis located in the mediastinum testis, to the efferent ducts, and then to the epididymis where newly created sperm cells mature (see spermatogenesis). The sperm move into the vas deferens, and are eventually expelled through the urethra and out of the urethral orifice through muscular contractions.

However, most fish do not possess seminiferous tubules. Instead, the sperm are produced in spherical structures called sperm ampullae. These are seasonal structures, releasing their contents during the breeding season, and then being reabsorbed by the body. Before the next breeding season, new sperm ampullae begin to form and ripen. The ampullae are otherwise essentially identical to the seminiferous tubules in higher vertebrates, including the same range of cell types.[2]

In terms of spermatogonia distribution, the structure of teleosts testes has two types: in the most common, spermatogonia occur all along the seminiferous tubules, while in Atherinomorph fish they are confined to the distal portion of these structures. Fish can present cystic or semi-cystic spermatogenesis in relation to the release phase of germ cells in cysts to the seminiferous tubules lumen.[1]

Ovaries[edit]

3. Ovary of a female Atlantic cod

Many of the features found in ovaries are common to all vertebrates, including the presence of follicular cells and tunica albuginea There may be hundreds or even millions of fertile eggs present in the ovary of a fish at any given time. Fresh eggs may be developing from the germinal epithelium throughout life. Corpora lutea are found only in mammals, and in some elasmobranch fish; in other species, the remnants of the follicle are quickly resorbed by the ovary.[2] The ovary of teleosts is often contains a hollow, lymph-filled space which opens into the oviduct, and into which the eggs are shed.[2] Most normal female fish have two ovaries. In some elasmobranchs, only the right ovary develops fully. In the primitive jawless fish, and some teleosts, there is only one ovary, formed by the fusion of the paired organs in the embryo.[2]

Fish ovaries may be of three types: gymnovarian, secondary gymnovarian or cystovarian. In the first type, the oocytes are released directly into the coelomic cavity and then enter the ostium, then through the oviduct and are eliminated. Secondary gymnovarian ovaries shed ova into the coelom from which they go directly into the oviduct. In the third type, the oocytes are conveyed to the exterior through the oviduct.[3] Gymnovaries are the primitive condition found in lungfish, sturgeon, and bowfin. Cystovaries characterize most teleosts, where the ovary lumen has continuity with the oviduct.[1] Secondary gymnovaries are found in salmonids and a few other teleosts.

Intromittent organs[edit]

This male mosquitofish has a gonopodium, an anal fin which functions as an intromittent organ[4][5]
This young male spinner shark has claspers, a modification to the pelvic fins which also function as intromittent organs

Male cartilaginous fishes (sharks and rays), as well as the males of some live-bearing ray finned fishes, have fins that have been modified to function as intromittent organs, reproductive appendages which allow internal fertilization. In ray finned fish they are called gonopodiums or andropodiums, and in cartilaginous fish they are called claspers.

Gonopodiums are found on the males of some species in the Anablepidae and Poeciliidae families. They are anal fins that have been modified to function as movable intromittent organs and are used to impregnate females with milt during mating. The third, fourth and fifth rays of the male's anal fin are formed into a tube-like structure in which the sperm of the fish is ejected.[6] When ready for mating, the gonopodium becomes erect and points forward towards the female. The male shortly inserts the organ into the sex opening of the female, with hook-like adaptations that allow the fish to grip onto the female to ensure impregnation. If a female remains stationary and her partner contacts her vent with his gonopodium, she is fertilized. The sperm is preserved in the female's oviduct. This allows females to fertilize themselves at any time without further assistance from males. In some species, the gonopodium may be half the total body length. Occasionally the fin is too long to be used, as in the "lyretail" breeds of Xiphophorus helleri. Hormone treated females may develop gonopodia. These are useless for breeding.

Similar organs with similar characteristics are found in other fishes, for example the andropodium in the Hemirhamphodon or in the Goodeidae.[7]

Claspers are found on the males of cartilaginous fishes. They are the posterior part of the pelvic fins that have also been modified to function as intromittent organs, and are used to channel semen into the female's cloaca during copulation. The act of mating in sharks usually includes raising one of the claspers to allow water into a siphon through a specific orifice. The clasper is then inserted into the cloaca, where it opens like an umbrella to anchor its position. The siphon then begins to contract expelling water and sperm.[8][9]

Physiology[edit]

Oogonia development in teleosts fish varies according to the group, and the determination of oogenesis dynamics allows the understanding of maturation and fertilisation processes. Changes in the nucleus, ooplasm, and the surrounding layers characterize the oocyte maturation process.[1]

Postovulatory follicles are structures formed after oocyte release; they do not have endocrine function, present a wide irregular lumen, and are rapidly reabsorbed in a process involving the apoptosis of follicular cells. A degenerative process called follicular atresia reabsorbs vitellogenic oocytes not spawned. This process can also occur, but less frequently, in oocytes in other development stages.[1]

Some fish are hermaphrodites, having both testes and ovaries either at different phases in their life cycle or, as in hamlets, have them simultaneously.

Reproductive strategies[edit]

See also: Spawning, Ichthyoplankton

In fish, fertilisation of eggs can be either external or internal. In many species of fish, fins have been modified to allow Internal fertilisation. Similarly, development of the embryo can be external or internal, although some species show a change between the two at various stages of embryo development. Lode described reproductive strategies in terms of the development of the zygote and the interrelationship with the parents; there are five classifications - ovuliparity, oviparity, ovo-viviparity, histotrophic viviparity and hemotrophic viviparity.[10]

Ovuliparity[edit]

Ovuliparity means the female lays unfertilised eggs (ova), which must then be externally fertilised. Examples of ovuliparous fish include salmon, goldfish, cichlids, tuna and eels. In the majority of these species, fertilisation takes place outside the mother's body, with the male and female fish shedding their gametes into the surrounding water.

Oviparity[edit]

Main article: Oviparity

Oviparity is where fertilisation occurs internally and so the female sheds zygotes (or newly developing embryos)into the water, often with important outer tissues added. Over 97% of all known fish are oviparous,[11] In oviparous fish, internal fertilisation equires the male to use some sort of intromittent organ to deliver sperm into the genital opening of the female. Examples include the oviparous sharks, such as the horn shark, and oviparous rays, such as skates. In these cases, the male is equipped with a pair of modified pelvic fins known as claspers.

Marine fish can produce high numbers of eggs which are often released into the open water column. The eggs have an average diameter of 1 millimetre (0.039 in). The eggs are generally surrounded by the extraembryonic membranes but do not develop a shell, hard or soft, around these membranes. Some fish have thick, leathery coats, especially if they must withstand physical force or desiccation. These type of eggs can also be very small and fragile.

The newly hatched young of oviparous fish are called larvae. They are usually poorly formed, carry a large yolk sac (for nourishment) and are very different in appearance from juvenile and adult specimens. The larval period in oviparous fish is relatively short (usually only several weeks), and larvae rapidly grow and change appearance and structure (a process termed metamorphosis) to become juveniles. During this transition larvae must switch from their yolk sac to feeding on zooplankton prey, a process which depends on typically inadequate zooplankton density, starving many larvae.

Ovoviviparity[edit]

Main article: Ovoviviparity

In ovoviviparous fish the eggs develop inside the mother's body after internal fertilisation but receive little or no nourishment directly from the mother, depending instead on the yolk. Each embryo develops in its own egg. Familiar examples of ovoviviparous fish include guppies, angel sharks, and coelacanths.

Viviparity[edit]

Main article: Viviparity

There are two types of viviparity, differentiated by how the offspring gain their nutrients.

  • Histotrophic (tissue eating) viviparity means embryos develop in the female’s oviducts but obtain nutrients by consuming other ova (oophagy) or zygotes. This has been observed primarily among sharks such as the shortfin mako and porbeagle, but is known for a few bony fish as well such as the halfbeak Nomorhamphus ebrardtii.[12] An even more unusual mode of vivipary is adelphophagy or intrauterine cannibalism in which the largest embryos eat weaker, smaller unborn siblings. This behavior is also most commonly found among sharks such as the grey nurse shark, but has also been reported for Nomorhamphus ebrardtii.[12]
  • Hemotrophic (blood eating) viviparity means embryos develop in the female’s (or male's) oviduct and nutrients are provided directly by the parent, typically via a structure similar to, or analogous to the placenta seen in mammals. Examples of hemotrophic fish include the surf-perches, splitfins, lemon shark, seahorses and pipefish.

Aquarists commonly refer to ovoviviparous and viviparous fish as livebearers.

Hermaphroditism[edit]

Female groupers change their sex to male if no male is available
An anemone fish couple guarding their anemone. If the female dies, a juvenile male moves in, and the resident male changes sex.
Female anglerfish, Haplophryne mollis, with atrophied males attached
Parthenogenesis was first described among vertebrates in the Amazon molly
Main article: Hermaphroditism

Hermaphroditism occurs when a given individual in a species possesses both male and female reproductive organs, or can alternate between possessing first one, and then the other. Hermaphroditism is common in invertebrates but rare in vertebrates. It can be contrasted with gonochorism, where each individual in a species is either male or female, and remains that way throughout their lives. Most fish are gonochorists, but hermaphroditism is known to occur in 14 families of teleost fishes.[13]

Usually hermaphrodites are sequential, meaning they can switch sex, usually from female to male (protogyny). This can happen if a dominant male is removed from a group of females. The largest female in the harem can switch sex over a few days and replace the dominant male.[13] This is found amongst coral reef fishes such as groupers, parrotfishes and wrasses. It is less common for a male to switch to a female (protandry).[14]:162 As an example, most wrasses are protogynous hermaphrodites within a haremic mating system.[15][16] Hermaphroditism allows for complex mating systems. Wrasses exhibit three different mating systems: polygynous, lek-like, and promiscuous mating systems.[17] Group spawning and pair spawning occur within mating systems. The type of spawning that occurs depends on male body size.[16] Labroids typically exhibit broadcast spawning, releasing high amounts of planktonic eggs, which are broadcast by tidal currents; adult wrasses have no interaction with offspring.[18] Wrasse of a particular subgroup of the Labridae family Labrini do not exhibit broadcast spawning.

Less commonly hermaphrodites can be synchronous, meaning they simultaneously possess both ovaries and testicles and can function as either sex at any one time. Black hamlets "take turns releasing sperm and eggs during spawning. Because such egg trading is advantageous to both individuals, hamlets are typically monogamous for short periods of time–an unusual situation in fishes."[19] The sex of many fishes is not fixed, but can change with physical and social changes to the environment where the fish lives.[20]

Particularly among fishes, hermaphroditism can pay off in situations where one sex is more likely to survive and reproduce, perhaps because it is larger.[21] Anemone fishes are sequential hermaphrodites which are born as males, and become females only when they are mature. Anemone fishes live together monogamously in a anemone, protected by the anemone stings. The males do not have to compete with other males, and female anemone fish are typically larger. When a female dies a juvenile (male) anemone fish moves in, and "the resident male then turns into a female and reproductive advantages of the large female–small male combination continue".[22] In other fishes sex changes are reversible. For example, if some gobies are grouped by sex (male or female), some will switch sex.[14]:164[21]

Sexual parasitism[edit]

Some anglerfish, like those of the deep sea ceratioid group, employ an unusual mating method. Because individuals are locally rare, encounters are also very rare. Therefore, finding a mate is problematic. When scientists first started capturing ceratioid anglerfish, they noticed that all of the specimens were female. These individuals were a few centimetres in size and almost all of them had what appeared to be parasites attached to them. It turned out that these "parasites" were highly reduced male ceratioids. This indicates the anglerfish use a polyandrous mating system.

The methods by which the anglerfish locate mates are variable. Some species have minute eyes unfit for identifying females visually, while others have underdeveloped nostrils, making it unlikely that they effectively find females using olfaction.[23] When a male finds a female, he bites into her skin, and releases an enzyme that digests the skin of his mouth and her body, fusing the pair down to the blood-vessel level.[24] The male becomes dependent on the female host for survival by receiving nutrients via their shared circulatory system, and provides sperm to the female in return. After fusing, males increase in volume and become much larger relative to free-living males of the species. They live and remain reproductively functional as long as the female lives, and can take part in multiple spawnings.[23] This extreme sexual dimorphism ensures, when the female is ready to spawn, she has a mate immediately available.[25] Multiple males can be incorporated into a single individual female with up to eight males in some species, though some taxa appear to have a one male per female rule.[23]

One explanation for the evolution of sexual parasitism is that the relative low density of females in deep-sea environments leaves little opportunity for mate choice among anglerfish. Females remain large to accommodate fecundity, as is evidenced by their large ovaries and eggs. Males would be expected to shrink to reduce metabolic costs in resource-poor environments and would develop highly specialized female-finding abilities. If a male manages to find a female parasitic attachment, then it is ultimately more likely to improve lifetime fitness relative to free living, particularly when the prospect of finding future mates is poor. An additional advantage to parasitism is that the male’s sperm can be used in multiple fertilizations, as he stays always available to the female for mating. Higher densities of male-female encounters might correlate with species that demonstrate facultative parasitism or simply use a more traditional temporary contact mating.[26]

Parthenogenesis[edit]

Main article: Parthenogenesis

Parthenogenesis is a form of asexual reproduction in which growth and development of embryos occur without fertilization. In animals, parthenogenesis means development of an embryo from an unfertilized egg cell. The first all-female (unisexual) reproduction in vertebrates was described in the Amazon molly in 1932.[27] Since then at least 50 species of unisexual vertebrate have been described, including at least 20 fish, 25 lizards, a single snake species, frogs, and salamanders.[28] As with all types of asexual reproduction, there are both costs (low genetic diversity and therefore susceptibility to adverse mutations that might occur) and benefits (reproduction without the need for a male) associated with parthenogenesis.

Parthenogenesis in sharks has been confirmed in the bonnethead[29] and zebra shark.[30] Other, usually sexual species, may occasionally reproduce parthenogenetically, and the hammerhead and blacktip sharks[31] are recent additions to the known list of facultative parthenogenetic vertebrates.

A special case of parthenogenesis is gynogenesis. In this type of reproduction, offspring are produced by the same mechanism as in parthenogenesis, however, the egg is stimulated to develop simply by the presence of sperm - the sperm cells do not contribute any genetic material to the offspring. Because gynogenetic species are all female, activation of their eggs requires mating with males of a closely related species for the needed stimulus. The Amazon molly, (pictured), reproduces by gynogenesis.

Others[edit]

The elkhorn sculpin (Alcichthys elongatus) is a marine teleost with a unique reproductive mode called “internal gametic association”. Sperm are introduced into the ovary by copulation and then enter the micropylar canal of ovulated eggs in the ovarian cavity. However, actual sperm-egg fusion does not occur until the eggs have been released into sea water.[32]

Sexual strategies[edit]

Spawning strategies[edit]

Main article: Spawning strategies

Spawning grounds[edit]

Main article: Spawning ground

Examples[edit]

Female goldfish spawn (discharge) eggs into the water, encouraged by male goldfish who simultaneously discharge sperm which externally fertilizes the eggs
Within two or three days, the vulnerable goldfish eggs hatch into larvae, and rapidly develop into fry
Goldfish

Goldfish, like all cyprinids, are egg-layers. They usually start breeding after a significant temperature change, often in spring. Males chase females, prompting them to release their eggs by bumping and nudging them. As the female goldfish spawns her eggs, the male goldfish stays close behind fertilizing them. Their eggs are adhesive and attach to aquatic vegetation. The eggs hatch within 48 to 72 hours. Within a week or so, the fry begins to assume its final shape, although a year may pass before they develop a mature goldfish colour; until then they are a metallic brown like their wild ancestors. In their first weeks of life, the fry grow quickly—an adaptation born of the high risk of getting devoured by the adult goldfish.

Carp

A member of the Cyprinidae family, carp spawn in times between April and August, largely dependent upon the climate and conditions they live in. Oxygen levels of the water, availability of food, size of each fish, age, number of times the fish has spawned before and water temperature are all factors known to effect when and how many eggs each carp will spawn at any one time.[33]

Siamese fighting fish

Prior to spawning, male Siamese fighting fish build bubble nests of varying sizes at the surface of the water. When a male becomes interested in a female, he will flare his gills, twist his body, and spread his fins. The female darkens in colour and curves her body back and forth. The act of spawning takes place in a "nuptial embrace" where the male wraps his body around the female, each embrace resulting in the release of 10-40 eggs until the female is exhausted of eggs. The male, from his side, releases milt into the water and fertilization takes place externally. During and after spawning, the male uses his mouth to retrieve sinking eggs and deposit them in the bubble nest (during mating the female sometimes assists her partner, but more often she will simply devour all the eggs that she manages to catch). Once the female has released all of her eggs, she is chased away from the male's territory, as it is likely that she'll eat the eggs due to hunger.[34] The eggs then remain in the male's care. He keeps them in the bubble nest, making sure none fall to the bottom and repairing the nest as needed. Incubation lasts for 24–36 hours, and the newly hatched larvae remain in the nest for the next 2–3 days, until their yolk sacs are fully absorbed. Afterwards the fry leave the nest and the free-swimming stage begins.[35]

References[edit]

  1. ^ a b c d e Guimaraes-Cruz, Rodrigo J., Rodrigo J.; Santos, José E. dos; Santos, Gilmar B. (2005). "Gonadal structure and gametogenesis of Loricaria lentiginosa Isbrücker (Pisces, Teleostei, Siluriformes)". Rev. Bras. Zool. 22 (3): 556–564. doi:10.1590/S0101-81752005000300005. ISSN 0101-8175. 
  2. ^ a b c d e Romer, Alfred Sherwood; Parsons, Thomas S. (1977). The Vertebrate Body. Philadelphia, PA: Holt-Saunders International. pp. 385–386. ISBN 0-03-910284-X. 
  3. ^ Brito, M.F.G.; Bazzoli, N. (2003). "Reproduction of the surubim catfish (Pisces, Pimelodidae) in the São Francisco River, Pirapora Region, Minas Gerais, Brazil". Arquivo Brasileiro de Medicina Veterinária e Zootecnia 55 (5): 624–633. doi:10.1590/S0102-09352003000500018. ISSN 0102-0935. 
  4. ^ Masterson, J. "Gambusia affinis". Smithsonian Institution. Retrieved 21 October 2011. 
  5. ^ Kuntz, Albert (1913). "Notes on the Habits, Morphology of the Reproductive Organs, and Embryology of the Viviparous Fish Gambusia affinis". Bulletin of the United States Bureau of Fisheries (Department of Commerce) 33: 181–190. 
  6. ^ Kapoor BG and Khanna B (2004) Ichthyology Handbook pp. 497–498, Springer Science & Business Media. ISBN 9783540428541.
  7. ^ Helfman G, Collette BB, Facey DH and Bowen BW (2009) The Diversity of Fishes: Biology, Evolution, and Ecology p. 35, Wiley-Blackwell. ISBN 978-1-4051-2494-2.
  8. ^ "System glossary". FishBase. Retrieved 2013-02-15. 
  9. ^ Heinicke, Matthew P.; Naylor, Gavin J. P.; Hedges, S. Blair (2009). The Timetree of Life: Cartilaginous Fishes (Chondrichthyes). Oxford University Press. p. 320. ISBN 0191560154. 
  10. ^ Lodé, T. (2012). "Oviparity or viviparity? That is the question…". Reproductive Biology 12 (3): 259–264. Retrieved November 4, 2014. 
  11. ^ Peter Scott: Livebearing Fishes, p. 13. Tetra Press 1997. ISBN 1-56465-193-2
  12. ^ a b Meisner, A & Burns, J: Viviparity in the Halfbeak Genera Dermogenys and Nomorhamphus (Teleostei: Hemiramphidae). Journal of Morphology 234, pp. 295–317, 1997
  13. ^ a b Shapiro DY (1984) "Sex reversal and sociodemographics processes in coral reef fishes" Pages 103–116 in GW Potts and RK Wootoon, eds., Fish reproduction: Strategies and tactics, Academic Press.
  14. ^ a b Moyle PB and Cech JJ (2004) Fishes, An Introduction to Ichthyology. 5th Ed, Benjamin Cummings. ISBN 978-0-13-100847-2
  15. ^ Robertson, D.R.; R.R. Warner. "Sexual patterns in the labroid fishes of the Western Caribbean II: the parrotfishes (Scaridae)". Smithsonian Contributions to Zoology 255: 1–26. doi:10.5479/si.00810282.255. 
  16. ^ a b Kazancioglu, E.; S.H. Alonzo. "A comparative analysis of sex change in Labridae supports the size advantage hypothesis". Evolution 64 (8): 2254–2264. doi:10.1111/j.1558-5646.2010.01016.x. PMID 20394662. 
  17. ^ Colin, P.L.; L. J. Bell. "Aspects of the spawning of labrid and scarid fishes (Pisces, Labroidei) at Enewetak Atoll, Marshall Islands with notes on other families (corrected reprint.)". Environmental Biology of Fishes 33 (3): 330–345. doi:10.1007/BF00005881. 
  18. ^ Hanel, R.; M. W. Westneat and C. Sturmbauer. "Phylogenetic relationships, evolution of broodcare behavior, and geographic speciation in the Wrasse tribe Labrini". Journal of Molecular Evolution 55 (6): 776–789. doi:10.1007/s00239-002-2373-6. PMID 12486536. 
  19. ^ Fischer EA, Peterson CW (1987). "The evolution of sexuality in the seabasses". BioScience 37 (7): 482–489. doi:10.2307/1310420. 
  20. ^ Chan STH and Yeung WSB (1983) "Sex control and sex reversal in fish under natural conditions". Pages 171–222 in WS Hoar, DJ Randall and EM Donaldson, eds., Fish physiology 9B: Reproduction, behavior and fertility control. Academic Press.
  21. ^ a b Kuwamura, Tetsuo; Nakashima, Yasuhiro (1998). Environmental Biology of Fishes 52: 125–135. doi:10.1023/A:1007389519702. 
  22. ^ Fricke, Hans; Fricke, Simone (1977). "Monogamy and sex change by aggressive dominance in coral reef fish". Nature 266 (5605): 830–832. Bibcode:1977Natur.266..830F. doi:10.1038/266830a0. PMID 865603. 
  23. ^ a b c Pietsch, Theodore W. (25 August 2005). "Dimorphism, parasitism, and sex revisited: modes of reproduction among deep-sea ceratioid anglerfishes (Teleostei: Lophiiformes)". Ichthyological Research 52 (3): 207–236. doi:10.1007/s10228-005-0286-2. 
  24. ^ Gould, Stephen Jay (1983). Hen's Teeth and Horse's Toes. New York: W. W. Norton & Company. ISBN 0-393-01716-8. 
  25. ^ Theodore W. Pietsch. "Precocious sexual parasitism in the deep sea ceratioid anglerfish, Cryptopsaras couesi Gill". Archived from the original on 28 August 2008. Retrieved 31 July 2008. 
  26. ^ Miya, Masaki; Pietsch, Theodore W; Orr, James W; Arnold, Rachel J; Satoh, Takashi P; Shedlock, Andrew M; Ho, Hsuan-Ching; Shimazaki, Mitsuomi; Yabe, Mamoru; Nishida, Mutsumi (1 January 2010). "Evolutionary history of anglerfishes (Teleostei: Lophiiformes): a mitogenomic perspective". BMC Evolutionary Biology 10 (1): 58. doi:10.1186/1471-2148-10-58. PMC 2836326. PMID 20178642. 
  27. ^ Hubbs, C. L.; Hubbs, L. C. (1932). "Apparent parthenogenesis in nature, in a form of fish of hybrid origin". Science 76 (1983): 628–630. doi:10.1126/science.76.1983.628. PMID 17730035. 
  28. ^ Vrijenhoek, R.C., R.M. Dawley, C.J. Cole, and J.P. Bogart. 1989. A list of the known unisexual vertebrates, pp. 19-23 in: Evolution and Ecology of Unisexual Vertebrates. R.M. Dawley and J.P. Bogart (eds.) Bulletin 466, New York State Museum, Albany, New York
  29. ^ Chapman, Demian D.; Shivji, Mahmood S.; Louis, Ed; Sommer, Julie; Fletcher, Hugh; Prodöhl, Paulo A. (2007). "Virgin birth in a hammerhead shark". Biology Letters 3 (4): 425–427. doi:10.1098/rsbl.2007.0189. PMC 2390672. PMID 17519185. 
  30. ^ Robinson, D. P.; Baverstock, W.; Al-Jaru, A.; Hyland, K.; Khazanehdari, K. A. (2011). "Annually recurring parthenogenesis in a zebra shark Stegostoma fasciatum". Journal of Fish Biology 79 (5): 1376–1382. doi:10.1111/j.1095-8649.2011.03110.x. PMID 22026614. 
  31. ^ Chapman, D. D.; Firchau, B.; Shivji, M. S. (2008). "Parthenogenesis in a large-bodied requiem shark, the blacktip". Journal of Fish Biology 73 (6): 1473–1477. doi:10.1111/j.1095-8649.2008.02018.x. 
  32. ^ Koya, Y., Munehara, H. and Takano, K. (2002). "Sperm storage and motility in the ovary of the marine sculpin Alcichthys alcicornis (Teleostei: Scorpaeniformes), with internal gametic association". Journal of Experimental Zoology 292 (2): 145–155. doi:10.1002/jez.1150. Retrieved November 4, 2014. 
  33. ^ http://www.carp.me.uk Carp Spawning Information
  34. ^ Leong, Paul (2004). http://www.cbsbettas.org/doc/articles/Tips_on_Spawning_Bettas.htm. Retrieved on March 13, 2009.
  35. ^ Rainwate FL and Miller EJ (1967) "Courtship and reproductive behavior of the Siamese fighting fish, Betta splendens Regan" Proceedings of the Oklahoma Academy of Science, Oklahoma State University.

Further references[edit]

External links[edit]