Calf-intestinal alkaline phosphatase: Difference between revisions
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{{enzyme|EC_number=3.1.3.1|GO_code=0004035}} |
{{enzyme|EC_number=3.1.3.1|GO_code=0004035}} |
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'''Calf-intestinal alkaline phosphatase''' ('''CIAP'''/'''CIP''') is a type of [[alkaline phosphatase]] that catalyzes the removal of phosphate |
'''Calf-intestinal alkaline phosphatase''' ('''CIAP'''/'''CIP''') is a type of [[alkaline phosphatase]] that catalyzes the removal of [[phosphate group]]s from the 5' end of DNA strands and [[phosphomonoesters]] from RNA.<ref name="sambrook CSHL" /><ref name="seeburg 270" /> This enzyme is frequently used in DNA [[sub-cloning]], as DNA fragments that lack the 5' phosphate groups cannot [[Ligation (molecular biology)|ligate]].<ref name="Ullrich 196" /> This prevents recircularization of the linearized DNA [[vector (molecular biology)|vector]] and improves the yield of the vector containing the appropriate insert. |
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==References== |
==References== |
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Revision as of 09:29, 25 February 2022
| Calf-intestinal alkaline phosphatase | |||||||
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| Identifiers | |||||||
| Organism | |||||||
| Symbol | ALPI | ||||||
| UniProt | P19111 | ||||||
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| Calf-intestinal alkaline phosphatase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.3.1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands and phosphomonoesters from RNA.[1][2] This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate.[3] This prevents recircularization of the linearized DNA vector and improves the yield of the vector containing the appropriate insert.
References
- ^ Sambrook J, Fritsch EF, Maniatis T (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.
- ^ Seeburg PH, Shine J, Martial JA, Baxter JD, Goodman HM (December 1977). "Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone". Nature. 270 (5637): 486–94. Bibcode:1977Natur.270..486S. doi:10.1038/270486a0. PMID 339105. S2CID 4196683.
- ^ Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter WJ, Goodman HM (June 1977). "Rat insulin genes: construction of plasmids containing the coding sequences". Science. 196 (4296). New York, N.Y.: 1313–9. Bibcode:1977Sci...196.1313U. doi:10.1126/science.325648. PMID 325648.
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