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'''''In vitro'' meat''', also known as '''cultured meat''', is an animal flesh product that has never been part of a complete, living animal. Several current research projects are growing ''in vitro'' meat experimentally, although no meat has yet been produced for public consumption.<ref name="time">{{cite news |last = Siegelbaum |first = D.J. |title = In Search of a Test-Tube Hamburger |publisher = ''[[Time (magazine)|Time]]'' |date = 2008-04-23 |url = http://www.time.com/time/health/article/0,8599,1734630,00.html?imw=Y |accessdate = 2009-04-30 }}</ref> The first-generation products will most likely be [[Ground meat|chopped meat]], and a long-term goal is to grow fully developed muscle tissue. Potentially, any [[animal]]'s muscle tissue could be grown through the ''in vitro'' process, even human. With the costs of conventional meat farming techniques [[2007–2008 world food price crisis|constantly increasing]] and the [[World population estimates|rising world population]] (reaching 8.9 billion people by the year 2050<ref>Data from [http://www.un.org/esa/population/publications/longrange2/WorldPop2300final.pdf UN WORLD POPULATION TO 2300]</ref>) ''in vitro'' meat may be one of several new technologies needed to maintain food supplies by the year 2050.<ref name="guardiancouk"/> Conventional meat production may simply become too expensive for the average consumer to support.<ref name="guardiancouk">[http://www.guardian.co.uk/environment/2010/aug/16/artificial-meat-food-royal-society Artificial food? Food for thought by 2050]</ref>
'''''In vitro'' meat''', also known as '''cultured meat''', is an animal flesh product that has never been part of a complete, living animal. Several current research projects are growing ''in vitro'' meat experimentally, although no meat has yet been produced for public consumption.<ref name="time">{{cite news |last = Siegelbaum |first = D.J. |title = In Search of a Test-Tube Hamburger |publisher = ''[[Time (magazine)|Time]]'' |date = 2008-04-23 |url = http://www.time.com/time/health/article/0,8599,1734630,00.html?imw=Y |accessdate = 2009-04-30 }}</ref> The first-generation products will most likely be [[Ground meat|chopped meat]], and a long-term goal is to grow fully developed muscle tissue. Potentially, any [[animal]]'s muscle tissue could be grown through the ''in vitro'' process, even human. With the costs of conventional meat farming techniques [[2007–2008 world food price crisis|constantly increasing]] and the [[World population estimates|rising world population]] (reaching 8.9 billion people by the year 2050<ref>Data from [http://www.un.org/esa/population/publications/longrange2/WorldPop2300final.pdf UN WORLD POPULATION TO 2300]</ref>) ''in vitro'' meat may be one of several new technologies needed to maintain food supplies by the year 2050.<ref name="guardiancouk"/> Conventional meat production may simply become too expensive for the average consumer to support.<ref name="guardiancouk">[http://www.guardian.co.uk/environment/2010/aug/16/artificial-meat-food-royal-society Artificial food? Food for thought by 2050]</ref>


A few scientists claim that this technology is ready for commercial use and simply needs a company to back it.<ref name="npr.org">{{Citation |last = Levine |first = Ketzel |author-link = Ketzel Levine |publisher=[[National Public Radio]] |title = Lab-Grown Meat a Reality, But Who Will Eat It? |date = 2008-05-20 |url = http://www.npr.org/templates/story/story.php?storyId=90235492 |accessdate = 2010-01-10}}</ref> Cultured meat is currently prohibitively expensive,<ref name="time"/> but it is anticipated that the cost could be reduced to about half as expensive as conventionally produced chicken.<ref name="no-kill carnivore">{{cite news |last= Temple |first= James |title = The Future of Food: The No-kill Carnivore |publisher = [[Portfolio.com]] |date = 2009-02-23 |url = http://www.portfolio.com/views/columns/dual-perspectives/2009/02/23/The-No-kill-Carnivore|accessdate = 2009-08-07 }}</ref><ref name = "Pharma Concepts">[http://www.new-harvest.org/img/files/culturedmeatecon.pdf Preliminary Economics Study of Cultured Meat], eXmoor Pharma Concepts, 2008</ref>
A few scientists claim that this technology is ready for commercial use and simply needs a company to back it.<ref name="npr.org">{{Citation |last = Levine |first = Ketzel |author-link = Ketzel Levine |publisher=[[National Public Radio]] |title = Lab-Grown Meat a Reality, But Who Will Eat It? |date = 2008-05-20 |url = http://www.npr.org/templates/story/story.php?storyId=90235492 |accessdate = 2010-01-10}}</ref> Cultured meat is currently prohibitively expensive,<ref name="time"/> but it is anticipated that the cost could be reduced to about twice as expensive as conventionally produced chicken.<ref name="no-kill carnivore">{{cite news |last= Temple |first= James |title = The Future of Food: The No-kill Carnivore |publisher = [[Portfolio.com]] |date = 2009-02-23 |url = http://www.portfolio.com/views/columns/dual-perspectives/2009/02/23/The-No-kill-Carnivore|accessdate = 2009-08-07 }}</ref><ref name = "Pharma Concepts">[http://www.new-harvest.org/img/files/culturedmeatecon.pdf Preliminary Economics Study of Cultured Meat], eXmoor Pharma Concepts, 2008</ref>


''In vitro'' meat should not be confused with [[Meat analogue|imitation meat]], which is a [[Vegetarian cuisine|vegetarian food]] product produced from [[vegetable]] [[protein]], usually from [[Soybean|soy]] or [[gluten]]. The terms "synthetic meat" and "artificial meat" may refer to either. ''In vitro'' meat has also been described, somewhat derisively, as "laboratory-grown" meat.
''In vitro'' meat should not be confused with [[Meat analogue|imitation meat]], which is a [[Vegetarian cuisine|vegetarian food]] product produced from [[vegetable]] [[protein]], usually from [[Soybean|soy]] or [[gluten]]. The terms "synthetic meat" and "artificial meat" may refer to either. ''In vitro'' meat has also been described, somewhat derisively, as "laboratory-grown" meat.

Revision as of 15:33, 28 June 2011

In vitro meat, also known as cultured meat, is an animal flesh product that has never been part of a complete, living animal. Several current research projects are growing in vitro meat experimentally, although no meat has yet been produced for public consumption.[1] The first-generation products will most likely be chopped meat, and a long-term goal is to grow fully developed muscle tissue. Potentially, any animal's muscle tissue could be grown through the in vitro process, even human. With the costs of conventional meat farming techniques constantly increasing and the rising world population (reaching 8.9 billion people by the year 2050[2]) in vitro meat may be one of several new technologies needed to maintain food supplies by the year 2050.[3] Conventional meat production may simply become too expensive for the average consumer to support.[3]

A few scientists claim that this technology is ready for commercial use and simply needs a company to back it.[4] Cultured meat is currently prohibitively expensive,[1] but it is anticipated that the cost could be reduced to about twice as expensive as conventionally produced chicken.[5][6]

In vitro meat should not be confused with imitation meat, which is a vegetarian food product produced from vegetable protein, usually from soy or gluten. The terms "synthetic meat" and "artificial meat" may refer to either. In vitro meat has also been described, somewhat derisively, as "laboratory-grown" meat.

The process

Most meat is animal muscle. The process of developing in vitro meat involves taking muscle cells and applying a protein that helps the cells to grow into large portions of meat.[1] Once the initial cells have been obtained, additional animals would not be needed – akin to the production of yogurt cultures.[7]

There are, loosely, two approaches for production of in vitro meat: loose muscle cells and structured muscle, the latter one being vastly more challenging than the former. Muscles consist of muscle fibers, long cells with multiple nuclei. They don't proliferate by themselves, but arise when precursor cells fuse. Precursor cells can be embryonic stem cells or satellite cells, specialized stem cells in muscle tissue. Theoretically, it is relatively simple to culture them in a bioreactor and then make them fuse. For the growth of real muscle, however, the cells should grow "on the spot," which requires a perfusion system akin to a blood supply to deliver nutrients and oxygen close to the growing cells, as well as to remove the waste products. In addition, other cell types, such as adipocytes, need to be grown, and chemical messengers should provide clues to the growing tissue about the structure. Lastly, muscle tissue needs to be physically stretched or "exercised" to properly develop.[1]

In vitro meat does not necessarily involve genetic engineering, a common misconception. In fact, the cells involved are natural cells which would grow in the normal method.[1]

History

Modern research into in vitro meat arose out of experiments conducted by NASA, attempting to find improved forms of long-term food for astronauts in space.[8] The technique was approved by the U.S. Food and Drug Administration (FDA) in 1995,[9] and NASA has been conducting experiments since 2001, producing in vitro meat from turkey cells.[10][11] The first edible form was produced by the NSR/Touro Applied BioScience Research Consortium in 2000: goldfish cells grown to resemble fish fillets.[1][5][12]

In 2001, dermatologist Wiete Westerhof from the University of Amsterdam, medical doctor Willem van Eelen, and businessman Willem van Kooten announced that they had filed for a worldwide patent on a process to produce in vitro meat.[13] In the process, a matrix of collagen is seeded with muscle cells, which are then bathed in a nutritious solution and induced to divide. [14] Scientists in Amsterdam study the culture medium, while the University of Utrecht studies the proliferation of muscle cells, and the Eindhoven University of Technology is researching bioreactors.[14] Van Eelen said that he had thought of the idea of in vitro meat for years, since he was held in a Japanese POW camp.

Jon F. Vein of the United States has also secured a patent (U.S. patent 6,835,390) for the production of tissue-engineered meat for human consumption, wherein muscle and fat cells would be grown in an integrated fashion to create food products such as beef, poultry and fish.[15]

The first peer-reviewed journal article published on the subject of laboratory-grown meat appeared in a 2005 issue of Tissue Engineering.[8] Of course, the basic concept dates back further. Winston Churchill said in the 1930s, "Fifty years hence, we shall escape the absurdity of growing a whole chicken in order to eat the breast or wing, by growing these parts separately under a suitable medium."[10]

In 2008, PETA offered a $1 million prize to the first company that brings lab-grown chicken meat to consumers by 2012.[4] The Dutch government has put US$4 million into experiments into in vitro meat.[10] The In Vitro Meat Consortium, a group formed by international researchers interested in the technology, held the first international conference on the production of in vitro meat, hosted by the Food Research Institute of Norway in April 2008, to discuss commercial possibilities.[1] Time Magazine declared in vitro meat production to be one of the 50 breakthrough ideas of 2009. [16] In November 2009, scientists from the Netherlands announced they had managed to grow meat in the laboratory using the cells from a live pig.[17]

Differences from conventional meat

Health

Large scale production of In vitro meat may require artificial growth hormones to be added to the culture for meat production,[18] which is not necessary in conventional meat production. No procedure has been presented to produce large scale in vitro meat without the use of antibiotics to prevent bacterial infections.

Because in vitro meat has yet to be placed on the market, the health risks have not yet been fully investigated. This question is one of the main focuses of scientists working on in vitro meat, and the aim is to produce healthier meat than conventional meat, most notably by reducing its fat content and controlling nutrients.[dubiousdiscuss]

Researchers have suggested that omega-3 fatty acids could be added to in-vitro meat as a health bonus.[10] In a similar way, the omega-3 fatty acid content of conventional meat can also be increased by altering what the animals are fed.[19] Time Magazine has suggested that the in-vitro process may also decrease exposure of the meat to bacteria and disease.[1]

Artificiality

Although in vitro meat consists of natural meat cells, consumers may find such a high-technology approach to food production distasteful. Technology in cuisine tends to remain stagnant for a long period of time as people are often wary of new cooking methods. In vitro meat has been disparagingly described as Frankenmeat, reflecting a sentiment that it is unnatural and therefore wrong.[20]

If in vitro meat turns out to be different in appearance, taste, smell, texture, or other factors, it may not be commercially competitive with conventionally produced meat. The lack of fat and bone may also be a disadvantage, for these parts make appreciable culinary contributions. Many food items, such as surimi, designed to substitute for other ingredients (for reasons from morality to expense) have become independently sought out for their own properties.[21] However, the lack of bones and/or fat may make many traditional meats like Buffalo wings more palatable to small children.

Environmental

A few people have speculated that in-vitro meat might require fewer resources and produce less greenhouse gas and other waste than conventional meat production. This includes the patent holder for in-vitro meat,[14] the journalist Brendan I. Koerner,[22] and Hanna Tuomisto, a PhD student from Oxford University.[23] Margaret Mellon of the Union of Concerned Scientists, a science-based lobby group dedicated to environmental and social issues, speculates that the energy and fossil fuel requirements of large scale in-vitro meat production in a factory may make it more environmentally destructive than producing food off the land.[4]

Economic differences

The production of in-vitro meat is currently very expensive - about US$1 million for a piece of beef weighing 250 grams (0.55 lb)[1] - and it would take considerable investment to switch to large scale production. However, the In-Vitro Meat Consortium has estimated that with improvements to current technology there could be considerable reductions in the cost of in-vitro meat. They estimate that it could be produced for 3500€/tonne,[6] which is about twice the cost of unsubsidized conventional European chicken production.[5][6]

However, it is suggested that the high costs of food (which are projected to last until 2018[24] due to the decreased number of crop plantings prior to the 2009 growing season in Canada, the United States, and Europe) in developed countries are linked to packaging and advertising as opposed to the actual cost of the food product.[25] While the total number of cash crop plantings started to increase again at the 2009 planting season, prices have only begun to stabilize.[26][27] The UN's World Food Programme does not expect the price of wheat and corn - which are required to maintain livestock - to return to pre-2008 levels due to the rising cost of commodity goods in the emerging markets.[26][27]

Ethical considerations

Animal welfare groups are in favour of the production of in vitro meat because it does not have a nervous system and therefore cannot feel pain.[4][7][28]

Potential usage

The original NASA research on in vitro meat was intended for use on long space voyages or stays; it would be a sustainable food source alongside hydroponic or aeroponically grown vegetables.[dubiousdiscuss]

Research

Challenges

The science for in vitro meat is an outgrowth of the field of biotechnology known as tissue engineering. [29] The technology is simultaneously being developed along with other uses for tissue engineering such as helping those with muscular dystrophy and, similarly, growing transplant organs.[10][28] There are several obstacles to overcome if it has any chance of succeeding; at the moment, the most notable ones are scale and cost.[1][10]

  • Proliferation of muscle cells: Although it is not very difficult to make stem cells divide, for meat production it is necessary that they divide at a quick pace, producing the solid meat.[28] This requirement has some overlap with the medical branch of tissue engineering.
  • Culture medium: Proliferating cells need a food source to grow and develop. The growth medium should be a well-balanced mixture of ingredients and growth factors. Scientists have already identified possible growth media for turkey, [30] fish,[31] sheep [32] and pig [33] muscle cells. Depending on the motives of the researchers, the growth medium has additional requirements.
    • Commercial: The growth medium should be inexpensive to produce. A plant-based medium may be less expensive than fetal bovine serum.[28]
    • Environmental: The production of the growth medium shouldn't have a negative effect on the environment. This means that the production should be energetically favorable. Additionally, the ingredients should come from completely renewable sources. Minerals from mined sources are in this case not possible, nor are synthetically produced nutrients which use non-renewable sources.
    • Animal welfare: The growth medium should be devoid of animal sources (except for the initial "mining" of the original stem cells).[28]
    • Non-Allergenic: While plant based growth media are "more realistic," will be cheaper, and reduce possibility of infectious agents, there is also the possibility that plant-based growth media may cause allergic reactions to some consumers.[34]
  • Bioreactors: Nutrients and oxygen need to be delivered close to each growing cell, on the scale of millimeters. In animals this job is handled by blood vessels. A bioreactor should emulate this function in an efficient manner. The usual approach is the creation of a sponge-like matrix in which the cells can grow, and perfusing it with the growth medium.

Initiatives

Perhaps the first research into in vitro meat was performed by M. A. Benjaminson from Touro College.[35] His research group managed to grow muscle tissue from goldfish in a laboratory setting with several kinds of growth media.

In 2004, a group of researchers started the non-profit organization New Harvest, with the goal of promoting research into in vitro meat. Among the founders are Jason Matheny[10] and Vladimir Mironov. According to their website, cultured meat in a processed form, like sausages,[10] hamburgers,[10] or chicken nuggets, may become commercially available within several years.[10] One of the first places of businesses to accept this in vitro meat would be fast food restaurants.[dubiousdiscuss] Since they do not disclose which farmer or rancher provided them with food, in vitro meat in fast food restaurants is often seen as an inevitable advancement.[dubiousdiscuss]

In April 2005, a research project into cultured meat started in The Netherlands, and in 2008, it was reported that most research into in vitro meat is being conducted by Dutch scientific teams.[28] The research is carried out under the lead of Henk Haagsman, a meat science researcher at the University of Amsterdam, the Eindhoven University of Technology and Utrecht University, in cooperation with sausage manufacturer Stegeman. The Dutch government granted a two million euro subsidy for the project.[14]

On April 21, 2008, PETA announced a $1 million X-Prize style reward for the first group to successfully produce synthetic meat that is comparable to and commercially viable against naturally sourced meat products.[36]

In fiction

  • In Two Planets (original German title: Auf Zwei Planeten) (1897) by Kurd Lasswitz, "synthetic meat" is one of the varieties of synthetic food introduced on Earth by the Martians.
  • In Illegal Alien (1997) by Robert J. Sawyer, the monofilament used to cut artificial meat is a suspected murder weapon.
  • In the book Neuromancer (1984) by William Gibson, artificial meat, called vatgrown flesh, is mentioned as food sold in stores, cheaper than meat from living animals.
  • In The Space Merchants (1952) by Frederik Pohl and C.M. Kornbluth, artificial meat is grown in huge lumps tens of metres in diameter, workmen walking on top of it harvesting slices with big knives. The lump of meat is called "Chicken Little".
  • In the Terro-Human Future History stories (1952–84) by H. Beam Piper, "carniculture vats" were a common source of meat for food, even though livestock was raised for meat on worlds with appropriate biospheres. Several of Piper's protagonists (such as Lucas Trask in Space Viking) found "vat-grown" meat acceptable, but considered the "real thing" more palatable.
  • In Stars in My Pocket Like Grains of Sand (1984) by Samuel R. Delany, the main character's society uses vat-grown meat cultured from humans as the primary protein source. Interaction between this society and societies using 'natural' meat are (briefly) explored.
  • Claude Zidi's 1976 comedy film L'aile ou la cuisse starring Louis de Funès as top-notch gourmet and Julien Guiomar as the infamous Tricatel who secretly produces artificial food.
  • In Assimilating our Culture, That's What they're Doing!, one of Larry Niven's short stories set in The Draco Tavern, a man who visits the tavern is depressed by the fact that he has licensed his own genome to an alien race, who are mass-producing headless clones of him for the meat market on their home planet.
  • Two Bob the Angry Flower comic strips, The Vegetarian's Dilemma and Meat Sheets, touch on points relating to artificially produced meat.
  • In the short story collection "The State of the Art" by Iain M. Banks, one of the stories includes a party where the main course is vat grown meat from cells of notable human villains and megalomaniacs, with "stewed Idi Amin" and "Richard Nixon Burgers" among others.
  • In Margaret Atwood's Oryx and Crake, 'ChickieNobs' were chickens with only a mouth and a digestive tract that were genetically engineered for meat products.
  • In M.T. Anderson's Feed, meat is grown on farms as segments of the meat, e.g. steak.
  • Although not called "in vitro meat", the creation of zombification in the Xbox 360 game Dead Rising was the result of US scientists trying to mass produce meat for consumption. The engineered wasps that were to facilitate this escaped from the Santa Cabeza complex and "zombified" the populace, resulting in military intervention. Two of the survivors of that incident, Carlito and Isabella Keyes, started a new outbreak in the Southwest town of Willamette in revenge, where the game takes place.
  • A popular urban legend describes a genetically engineered vat-grown creature, dubbed "Animal 57", as the source of meat for various fast-food chains.
  • In Rudy Rucker's Ware Tetralogy almost all of the meat was tank grown, including human meat.
  • Meat on Star Trek is created by devices called replicators.
  • In Mass Effect 2 the Normandy's pilot remarks how he prefers natural cow meat over "vat-grown" beef.
  • In Crest of the Stars a space station's on-board meat culture facility is briefly displayed, and it is referred to provide a reliable source of sustenance for years (serving a crew of several dozen).
  • In an episode of the American Broadcasting Company (ABC) show Better Off Ted, they show scientists growing a "meat blob."

See also

References

  1. ^ a b c d e f g h i j Siegelbaum, D.J. (2008-04-23). "In Search of a Test-Tube Hamburger". Time. Retrieved 2009-04-30. {{cite news}}: Italic or bold markup not allowed in: |publisher= (help)
  2. ^ Data from UN WORLD POPULATION TO 2300
  3. ^ a b Artificial food? Food for thought by 2050
  4. ^ a b c d Levine, Ketzel (2008-05-20), Lab-Grown Meat a Reality, But Who Will Eat It?, National Public Radio, retrieved 2010-01-10
  5. ^ a b c Temple, James (2009-02-23). "The Future of Food: The No-kill Carnivore". Portfolio.com. Retrieved 2009-08-07. Cite error: The named reference "no-kill carnivore" was defined multiple times with different content (see the help page).
  6. ^ a b c Preliminary Economics Study of Cultured Meat, eXmoor Pharma Concepts, 2008
  7. ^ a b Raizel, Robin (2005-12-11). "In Vitro Meat". The New York Times. Retrieved 2009-08-07. {{cite news}}: Italic or bold markup not allowed in: |publisher= (help)
  8. ^ a b "Paper Says Edible Meat Can be Grown in a Lab on Industrial Scale" (Press release). University of Maryland. 2005-07-06. Retrieved 2008-10-12.
  9. ^ "Catachem, Inc Announces FDA Approval of UIBC In-Vitro Diagnostic (IVD) Chemistry Reagent Kit". BioPortfolio, verbatim, paid reprint of Catachem press release. 1995-02-21. Archived from the original on 2008-06-02. Retrieved 2008-12-07.
  10. ^ a b c d e f g h i j Macintyre, Ben (2007-01-20). "Test-tube meat science's next leap". The Australian. Retrieved 2011-11-26. {{cite news}}: Italic or bold markup not allowed in: |publisher= (help)
  11. ^ Webb, Sarah (2006-01-08). "Tissue Engineers Cook Up Plan for Lab-Grown Meat (The Year in Science: Technology)". Discover. Retrieved 2009-08-07. {{cite news}}: Italic or bold markup not allowed in: |publisher= (help)
  12. ^ Benjaminson, Morris (2001-12-05). "Featured Research at Touro: Growing Fish Fillets Outside the Fish". Touro College School of Health Sciences. Retrieved 2010-01-10. Advance announcement of paper's publication in Acta Astronautica (not found there, but note Journal articles below).
  13. ^ INDUSTRIAL SCALE PRODUCTION OF MEAT FROM IN VITRO CELL CULTURES, 1999-06-24 {{citation}}: Cite has empty unknown parameters: |issue-date= and |country-code= (help); Unknown parameter |description= ignored (help); Unknown parameter |inventor-first= ignored (help); Unknown parameter |inventor-last= ignored (help); Unknown parameter |inventor2-first= ignored (help); Unknown parameter |inventor2-last= ignored (help); Unknown parameter |inventor3-first= ignored (help); Unknown parameter |inventor3-last= ignored (help); Unknown parameter |patent-number= ignored (help)
  14. ^ a b c d van Eelen, Willem (2007-12-12). "Patent holder Willem van Eelen: 'In another five years meat will come out of the factory'". inVitroMeat Foundation, operated by Willem van Eelen, publishing what appears to be an English translation of an article in Dutch by Anouck Vrouwe (subscribers only) from Het Financieele Dagblad. {{cite news}}: External link in |publisher= (help); Italic or bold markup not allowed in: |publisher= (help)
  15. ^ Method for producing tissue engineered meat for consumption, 2001-11-16 {{citation}}: Unknown parameter |country-code= ignored (help); Unknown parameter |description= ignored (help); Unknown parameter |inventor-first= ignored (help); Unknown parameter |inventor-last= ignored (help); Unknown parameter |issue-date= ignored (help); Unknown parameter |patent-number= ignored (help)
  16. ^ "The 50 Best Inventions of 2009". Time Magazine. 2009-11-12.
  17. ^ Rogers, Lois (2009-11-29). "Scientists grow pork meat in a laboratory". The Sunday Times. London.
  18. ^ Edelman, P. D, D. C. McFarland, V. A. Mironov, and J. G. Matheny. 2005. In vitro-cultured meat production. Tissue Engineering 11(5–6): 659–662.
  19. ^ Azcona, J.O., Schang, M.J., Garcia, P.T., Gallinger, C., R. Ayerza (h), and Coates, W. (2008). "Omega-3 enriched broiler meat: The influence of dietary alpha-linolenic omega-3 fatty acid sources on growth, performance and meat fatty acid composition". Canadian Journal of Animal Science, Ottawa, Ontario, Canada, 88:257–269
  20. ^ Animal Liberation Front
  21. ^ Pigott, George M. (1990). Seafood. CRC Press. p. 236. ISBN 0824779223. {{cite book}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  22. ^ Koerner, Brendan I. (2008-05-20). "Will Lab-Grown Meat Save the Planet? Or is it only good for cows and pigs?". Slate. {{cite news}}: Italic or bold markup not allowed in: |publisher= (help)
  23. ^ Cheng, Maria (2010-01-15). "Stem Cells Turned Into Pork".
  24. ^ CNN "[Food prices rising across the world" 24 March 2008
  25. ^ The Farmer
  26. ^ a b Reuters
  27. ^ a b GMA News
  28. ^ a b c d e f Kruglinski, Susan (2008-09-22). "I'll Have My Burger Petri-Dish Bred, With Extra Omega-3". Discover. {{cite news}}: Italic or bold markup not allowed in: |publisher= (help); Unknown parameter |coauthor= ignored (|author= suggested) (help)
  29. ^ Edelman, P. D, D. C. McFarland, V. A. Mironov, and J. G. Matheny. 2005. In vitro-cultured meat production. Tissue Engineering 11(5–6): 659–662.
  30. ^ McFarland, D. C., Doumit, M. E., & Minshall, R. D. (1988). The turkey myogenic satellite cell: Optimization of in vitro proliferation and differentiation. Tissue and Cell, 20(6), 899–908.
  31. ^ Benjaminson, M. A., Gilchriest, J. A., & Lorenz, M. (2002). In vitro edible muscle protein production system (MPPS): Stage 1, fish. Acta Astronautica, 51(12), 879–889.
  32. ^ Dodson, M. V., & Mathison, B. A. (1988). Comparison of ovine and rat muscle-derived satellite cells: Response to insulin. Tissue and Cell, 20(6), 909–918.
  33. ^ Doumit, M. E., Cook, D. R., & Merkel, R. A. (1993). Fibroblast growth factor, epidermal growth factor, insulin-like growth factor and platelet-derived growth factor-BB stimulate proliferate of clonally derived porcine myogenic satellite cells. Journal of Cellular Physiology, 157(2), 326–332.
  34. ^ I. Datar, M. Betti, Possibilities for an in vitro meat production system, Innovative Food Science and Emerging Technologies 11 (2010) at 17.
  35. ^ In Search of a Test-Tube Hamburger
  36. ^ "The PETA Files – Lab Meat: Tastes Like a Million Bucks". PETA. 2008-04-21.

News coverage

Journal articles