The 2000 Supercoppa Italiana was a match contested by the Serie A 1999–2000 winners Lazio and the Coppa Italia 1999–2000 runners-up Internazionale. Since the Coppa Italia winners were also the newly appointed league champions, the Super Cup spot was given to the runners-up of the Coppa Italia, Internazionale.
The match resulted in a 4-3 win for Lazio.
- 90 minutes.
- 30 minutes of extra-time if necessary.
- Penalty shoot-out if scores still level.
- Seven named substitutes
- Maximum of 3 substitutions.