Smoove was born December 16, 1965 in Plymouth, North Carolina, and grew up in Mount Vernon, New York. He attended Norfolk State University in Virginia. He shortened his name, Jerry Brooks, to "J.B." and added "Smoove" as his last name when he began performing stand-up comedy. J.B. Smoove worked at a custom T-shirt shop (Mrs G's T's) where he printed T-shirts and model trains while developing material for his comedy routine.
In 2012, his first televised comedy special, JB Smoove: That's How I Dooz It, premiered on Comedy Central. The DVD of the special was released April 3, 2012. In 2013, Smoove voiced Hackus in The Smurfs 2. Smoove appeared in Movie 43 in the segment "The Proposition" alongside Anna Faris and Chris Pratt. He was the substitute co-host of the New York City morning talk show Good Day New York with Rosanna Scotto on April 9, 2012. He had a cameo as a grave digger in the episode of Louie entitled "Barney/Never". He also played a supporting role in Season 4, Episode 7 "The Vapora Sport" in the American sitcom The League on FX. He plays "Wheelchair Guy", with whom the main characters have recurring comedic run-ins. He also plays one of the "Replacers" for Call Of Duty: Black Ops II. He's also in the show Real Husbands of Hollywood. He hosts a talk show on the MSG Network called "Four Courses With J.B. Smoove". He provides the voice of Dr. Ray D'Angelo Harris, host of the Chakra Attack radio show in the video game Grand Theft Auto V. Smoove appeared in the 2013 film Dealin' with Idiots as "Coach Ted." In 2013, Smoove became a regular cast member of the CBS comedy The Millers, playing the character "Ray".
J.B. was the host of the reality series Last Comic Standing, which was revived by NBC for its eighth season in the summer of 2014.