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6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
PDB 3bif EBI.jpg
PDB rendering based on 3bif.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbol PFKFB4
External IDs OMIM605320 MGI2687284 HomoloGene48288 GeneCards: PFKFB4 Gene
EC number,
Species Human Mouse
Entrez 5210 270198
Ensembl ENSG00000114268 ENSMUSG00000025648
UniProt Q16877 Q6DTY7
RefSeq (mRNA) NM_004567 NM_001039215
RefSeq (protein) NP_004558 NP_766607
Location (UCSC) Chr 3:
48.52 – 48.56 Mb
Chr 9:
108.99 – 109.03 Mb
PubMed search [1] [2]

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 also known as PFKFB4 is an enzyme which in humans is encoded by the PFKFB4 gene.[1][2]


The bifunctional 6-phosphofructo-2-kinase (EC,6-bisphosphatase (EC (PFKFB) regulates the steady-state concentration of fructose 2,6-bisphosphate, an activator of a key regulatory enzyme of glycolysis, phosphofructokinase.
Latest research made by scientists at Cancer Research UK’s London Research Institute show that an enzyme called PFKFB4 is essential for balancing these two processes – making sure the cell’s energy needs are met without allowing free radicals to build up and trigger cell death. Study leader Dr. Almut Schulze, said: “Our study suggests that PFKFB4 acts to fine-tune the process by which cells convert glucose into energy. Blocking this enzyme in prostate cancer cells grown in the lab stalled growth and triggered a catastrophic build-up of free-radicals, suggesting that it could be a suitable drug target. Importantly, this route to energy production is common to many different types of cancer, suggesting that drugs to target it could potentially be used to treat a variety of cancers.”[3]


  1. ^ Sakai A, Kato M, Fukasawa M, Ishiguro M, Furuya E, Sakakibara R (March 1996). "Cloning of cDNA encoding for a novel isozyme of fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human placenta". J. Biochem. 119 (3): 506–11. doi:10.1093/oxfordjournals.jbchem.a021270. PMID 8830046. 
  2. ^ Manzano A, Pérez JX, Nadal M, Estivill X, Lange A, Bartrons R (March 1999). "Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene". Gene 229 (1-2): 83–9. doi:10.1016/S0378-1119(99)00037-2. PMID 10095107. 
  3. ^ Ros, Susana; Claudio R. Santos; Sofia Moco; Franziska Baenke; Gavin Kelly; Michael Howell; Nicola Zamboni; Almut Schulze (2012-03-20). "Functional Metabolic Screen Identifies 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 4 as an Important Regulator of Prostate Cancer Cell Survival". Cancer Discovery 2 (4): 328–343. doi:10.1158/2159-8290.CD-11-0234. 

Further reading[edit]