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=== Model organism ===
=== Model organism ===
''E. coli'' is frequently used as a [[model organism]] in [[microbiology]] studies. Cultivated strains (e.g. ''E. coli'' K12) are well-adapted to the laboratory environment, and, unlike [[wild type]] strains, have lost their ability to thrive in the intestine. Many lab strains lose their ability to form [[biofilm]]s.<ref>{{cite journal |author=Fux CA, Shirtliff M, Stoodley P, Costerton JW |title=Can laboratory reference strains mirror "real-world" pathogenesis? |journal=Trends Microbiol. |volume=13 |issue=2 |pages=58–63 |year=2005 |pmid=15680764 |doi=10.1016/j.tim.2004.11.001}}</ref><ref>{{cite journal |author=Vidal O, Longin R, Prigent-Combaret C, Dorel C, Hooreman M, Lejeune P |title=Isolation of an Escherichia coli K-12 mutant strain able to form biofilms on inert surfaces: involvement of a new ompR allele that increases curli expression |journal=J. Bacteriol. |volume=180 |issue=9 |pages=2442–9 |year=1998 |pmid=9573197 |doi= |pmc=107187}}</ref> These features protect wild type strains from [[antibody|antibodies]] and other chemical attacks, but require a large expenditure of energy and material resources.



In 1946, [[Joshua Lederberg]] and [[Edward Tatum]] first described the phenomenon known as [[bacterial conjugation]] using ''E. coli'' as a model bacterium,<ref>{{cite journal |last=Lederberg |first=Joshua |coauthors=E.L. Tatum |date=October 19, 1946 |title=Gene recombination in E. coli |journal=Nature |volume=158 |issue= 4016|page=558 |id= |url=http://profiles.nlm.nih.gov/BB/G/A/S/Z/_/bbgasz.pdf |doi=10.1038/158558a0|format=PDF|bibcode = 1946Natur.158..558L }} Source: [http://profiles.nlm.nih.gov/BB/G/A/S/Z/ National Library of Medicine – The Joshua Lederberg Papers]</ref> and it remains the primary model to study conjugation.<ref>{{cite book|title=Biological Activity of Crystal|pages=169}}</ref> ''E. coli'' was an integral part of the first experiments to understand [[bacteriophage|phage]] genetics,<ref>{{cite web |url=http://www.cshl.edu/History/phagecourse.html |title=The Phage Course – Origins |accessdate=2007-12-03 |last= |first= |coauthors= |year=2006 |work= |publisher=Cold Spring Harbor Laboratory}} {{Dead link|date=September 2010|bot=H3llBot}}</ref> and early researchers, such as [[Seymour Benzer]], used ''E. coli'' and phage T4 to understand the topography of gene structure.<ref name="pmid16590840">{{cite journal | author = Benzer S | title = ON THE TOPOGRAPHY OF THE GENETIC FINE STRUCTURE | journal = Proc. Natl. Acad. Sci. U.S.A. | volume = 47 | issue = 3 | pages = 403–15 | year = 1961 | month = March | pmid = 16590840 | pmc = 221592 | doi = 10.1073/pnas.47.3.403 |bibcode = 1961PNAS...47..403B }}</ref> Prior to Benzer's research, it was not known whether the gene was a linear structure, or if it had a branching pattern.<ref>{{cite web|title=Facts about E.Coli|url=http://eol.org/pages/972688/details|publisher=Encyclopedia of Life|accessdate=27 November 2013}}</ref>



''E. coli'' was one of the first organisms to have its genome sequenced; the complete genome of ''E. coli'' K12 was published by ''Science'' in 1997.<ref name="pmid9278503" />
''E. coli'' was one of the first organisms to have its genome sequenced; the complete genome of ''E. coli'' K12 was published by ''Science'' in 1997.<ref name="pmid9278503" />

Revision as of 14:25, 9 January 2014

"E. coli" redirects here. For protozoan parasite, see Entamoeba coli.
This article is about Escherichia coli as a species. For E. coli in medicine, see Pathogenic Escherichia coli. For E. coli in molecular biology, see Escherichia coli (molecular biology).

Escherichia coli
Scientific classification
Domain:
Bacteria
Kingdom:
Eubacteria
Phylum:
Proteobacteria
Class:
Gammaproteobacteria
Order:
Enterobacteriales
Family:
Enterobacteriaceae
Genus:
Escherichia
Species:
E. coli
Binomial name
Escherichia coli
(Migula 1895)
Castellani and Chalmers 1919
Synonyms

Bacillus coli communis Escherich 1885

Escherichia coli (/ˌɛʃ[invalid input: 'i-']ˈrɪkiə ˈkoʊlaɪ/;[1] commonly abbreviated E. coli) is a Gram-negative, facultative anaerobic, rod-shaped bacterium that is commonly found in the lower intestine of warm-blooded organisms (endotherms). Most E. coli strains are harmless, but some serotypes can cause serious food poisoning in humans, and are occasionally responsible for product recalls due to food contamination.[2][3] The harmless strains are part of the normal flora of the gut, and can benefit their hosts by producing vitamin K2,[4] and by preventing the establishment of pathogenic bacteria within the intestine.[5][6]

E. coli and related bacteria constitute about 0.1% of gut flora,[7] and fecal–oral transmission is the major route through which pathogenic strains of the bacterium cause disease. Cells are able to survive outside the body for a limited amount of time, which makes them ideal indicator organisms to test environmental samples for fecal contamination.[8][9] There is, however, a growing body of research that has examined environmentally persistent E. coli which can survive for extended periods outside of the host.[10]

The bacterium can be grown easily and inexpensively in a laboratory setting, and has been intensively investigated for over 60 years. E. coli is the most widely studied prokaryotic model organism, and an important species in the fields of biotechnology and microbiology, where it has served as the host organism for the majority of work with recombinant DNA.

Biology and biochemistry

Model of successive binary fission in E. coli

E. coli is Gram-negative, facultative anaerobic and non-sporulating.[11] Cells are typically rod-shaped, and are about 2.0 micrometers (μm) long and 0.25-1.0 μm in diameter, with a cell volume of 0.6–0.7 μm3.[12][13] It can live on a wide variety of substrates. E. coli uses mixed-acid fermentation in anaerobic conditions, producing lactate, succinate, ethanol, acetate and carbon dioxide. Since many pathways in mixed-acid fermentation produce hydrogen gas, these pathways require the levels of hydrogen to be low, as is the case when E. coli lives together with hydrogen-consuming organisms, such as methanogens or sulphate-reducing bacteria.[14]

Optimal growth of E. coli occurs at 37 °C (98.6 °F) but some laboratory strains can multiply at temperatures of up to 49 °C (120 °F).[15] Growth can be driven by aerobic or anaerobic respiration, using a large variety of redox pairs, including the oxidation of pyruvic acid, formic acid, hydrogen and amino acids, and the reduction of substrates such as oxygen, nitrate, fumarate, dimethyl sulfoxide and trimethylamine N-oxide.[16]

Strains that possess flagella are motile. The flagella have a peritrichous arrangement.[17]

E. coli and related bacteria possess the ability to transfer DNA via bacterial conjugation, transduction or transformation, which allows genetic material to spread horizontally through an existing population. This process led to the spread of the gene encoding shiga toxin from Shigella to E. coli O157:H7, carried by a bacteriophage.[18]

Diversity

Escherichia coli encompasses an enormous population of bacteria that exhibit a very high degree of both genetic and phenotypic diversity. Genome sequencing of a large number of isolates of E. coli and related bacteria shows that a taxonomic reclassification would be desirable. However, this has not been done, largely due to its medical importance[19] and E. coli remains one of the most diverse bacterial species: only 20% of the genome is common to all strains.[20]

In fact, from the evolutionary point of view, the members of genus Shigella (S. dysenteriae, S. flexneri, S. boydii, S. sonnei) should be classified as E. coli strains, a phenomenon termed taxa in disguise.[21] Similarly, other strains of E. coli (e.g. the K-12 strain commonly used in recombinant DNA work) are sufficiently different that they would merit reclassification.

A strain is a sub-group within the species that has unique characteristics that distinguish it from other strains. These differences are often detectable only at the molecular level; however, they may result in changes to the physiology or lifecycle of the bacterium. For example, a strain may gain pathogenic capacity, the ability to use a unique carbon source, the ability to take upon a particular ecological niche or the ability to resist antimicrobial agents. Different strains of E. coli are often host-specific, making it possible to determine the source of fecal contamination in environmental samples.[8][9] For example, knowing which E. coli strains are present in a water sample allows researchers to make assumptions about whether the contamination originated from a human, another mammal or a bird.

Serotypes

Main article: Pathogenic Escherichia coli § Serotypes

A common subdivision system of E. coli, but not based on evolutionary relatedness, is by serotype, which is based on major surface antigens (O antigen: part of lipopolysaccharide layer; H: flagellin; K antigen: capsule), e.g. O157:H7).[22] It is however common to cite only the serogroup, i.e. the O-antigen. At present about 190 serogroups are known.[23] The common laboratory strain has a mutation that prevents the formation of an O-antigen and is thus non-typeable.

Genome plasticity

Like all lifeforms, new strains of E. coli evolve through the natural biological processes of mutation, gene duplication and horizontal gene transfer, in particular 18% of the genome of the laboratory strain MG1655 was horizontally acquired since the divergence from Salmonella.[24] In microbiology, all strains of E. coli derive from E. coli K-12 or E. coli B strains. Some strains develop traits that can be harmful to a host animal. These virulent strains typically cause a bout of diarrhea that is unpleasant in healthy adults and is often lethal to children in the developing world.[25] More virulent strains, such as O157:H7 cause serious illness or death in the elderly, the very young or the immunocompromised.[5][25]

Neotype strain

E. coli is the type species of the genus (Escherichia) and in turn Escherichia is the type genus of the family Enterobacteriaceae, where it should be noted that the family name does not stem from the genus Enterobacter + "i" (sic.) + "aceae", but from "enterobacterium" + "aceae" (enterobacterium being not a genus, but an alternative trivial name to enteric bacterium).[26][27][28]

The original strain described by Escherich is believed to be lost, consequently a new type strain (neotype) was chosen as a representative: the neotype strain is ATCC 11775,[29] also known as NCTC 9001,[30] which is pathogenic to chickens and has an O1:K1:H7 serotype.[31] However, in most studies either O157:H7 or K-12 MG1655 or K-12 W3110 are used as a representative E.coli.

Phylogeny of Escherichia coli strains

Escherichia coli is a species. A large number of strains belonging to this species have been isolated and characterised. In addition to serotype (vide supra), they can be classified according to their phylogeny, i.e. the inferred evolutionary history, as shown below where the species is divided into six groups.[20][32]

The link between phylogenetic distance ("relatedness") and pathology is small, e.g. the O157:H7 serotype strains, which form a clade ("an exclusive group")—group E below—are all enterohaemorragic strains (EHEC), but not all EHEC strains are closely related. In fact, four different species of Shigella are nested among E. coli strains (vide supra), while Escherichia albertii and Escherichia fergusonii are outside of this group. All commonly used research strains of E. coli belong to group A and are derived mainly from Clifton's K-12 strain (λ⁺ F⁺; O16) and to a lesser degree from d'Herelle's Bacillus coli strain (B strain)(O7).

Salmonella enterica

E. albertii

E. fergusonii

Group B2

E. coli SE15 (O150:H5. Commensal)

E. coli E2348/69 (O127:H6. Enteropathogenic)

Group D

E. coli UMN026 (O17:K52:H18. Extracellular pathogenic)

E. coli (O19:H34. Extracellular pathogenic)

E. coli (O7:K1. Extracellular pathogenic)

group E

E. coli EDL933 (O157:H7 EHEC)

E. coli Sakai (O157:H7 EHEC)

E. coli EC4115 (O157:H7 EHEC)

E. coli TW14359 (O157:H7 EHEC)

Shigella
Group B1

E. coli E24377A (O139:H28. Enterotoxigenic)

E. coli E110019

E. coli 11368 (O26:H11. EHEC)

E. coli 11128 (O111:H-. EHEC)

E. coli IAI1 O8 (Commensal)

E. coli 53638 (EIEC)

E. coli SE11 (O152:H28. Commensal)

E. coli B7A

E. coli 12009 (O103:H2. EHEC)

E. coli GOS1 (O104:H4 EAHEC) German 2011 outbreak

E. coli E22

E. coli Olso O103

E. coli 55989 (O128:H2. Enteroaggressive)

Group A

E. coli ATCC8739 (O146. Crook's E.coli used in phage work in the 1950s)

K‑12 strain derivatives

E. coli K-12 W3110 (O16. λ⁻ F⁻ "wild type" molecular biology strain)

E. coli K-12 DH10b (O16. high electrocompetency molecular biology strain)

E. coli K-12 DH1 (O16. high chemical competency molecular biology strain)

E. coli K-12 MG1655 (O16. λ⁻ F⁻ "wild type" molecular biology strain)

E. coli BW2952 (O16. competent molecular biology strain)

E. coli 101-1 (O? H?. EAEC)

B strain derivatives

E. coli B REL606 (O7. high competency molecular biology strain)

E. coli BL21-DE3 (O7. expression molecular biology strain with T7 polymerase for pET system)

Genomics

The first complete DNA sequence of an E. coli genome (laboratory strain K-12 derivative MG1655) was published in 1997. It was found to be a circular DNA molecule 4.6 million base pairs in length, containing 4288 annotated protein-coding genes (organized into 2584 operons), seven ribosomal RNA (rRNA) operons, and 86 transfer RNA (tRNA) genes. Despite having been the subject of intensive genetic analysis for approximately 40 years, a large number of these genes were previously unknown. The coding density was found to be very high, with a mean distance between genes of only 118 base pairs. The genome was observed to contain a significant number of transposable genetic elements, repeat elements, cryptic prophages, and bacteriophage remnants.[33]

Today, over 60 complete genomic sequences of Escherichia and Shigella species are available. Comparison of these sequences shows a remarkable amount of diversity; only about 20% of each genome represents sequences present in every one of the isolates, while approximately 80% of each genome can vary among isolates.[20] Each individual genome contains between 4,000 and 5,500 genes, but the total number of different genes among all of the sequenced E. coli strains (the pan-genome) exceeds 16,000. This very large variety of component genes has been interpreted to mean that two-thirds of the E. coli pangenome originated in other species and arrived through the process of horizontal gene transfer.[34]

Proteomics

Full sets of E. coli proteins and their interactions have also been isolated and studied. A 2006 study purified 4,339 proteins from cultures of strain K-12 and found interacting partners for 2,667 proteins, many of which had unknown functions at the time.[35] A 2009 study found 5,993 interactions between proteins of the same E. coli strain though this data showed little overlap with that of the 2006 publication.[36]

Normal microbiota

E. coli normally colonizes an infant's gastrointestinal tract within 40 hours of birth, arriving with food or water or with the individuals handling the child. In the bowel, it adheres to the mucus of the large intestine. It is the primary facultative anaerobe of the human gastrointestinal tract.[37] (Facultative anaerobes are organisms that can grow in either the presence or absence of oxygen.) As long as these bacteria do not acquire genetic elements encoding for virulence factors, they remain benign commensals.[38]

Therapeutic use

Nonpathogenic Escherichia coli strain Nissle 1917 also known as Mutaflor and Escherichia coli O83:K24:H31 (known as Colinfant[39]) are used as a probiotic agents in medicine, mainly for the treatment of various gastroenterological diseases,[40] including inflammatory bowel disease.[41]

Role in disease

Main article: Pathogenic Escherichia coli

Virulent strains of E. coli can cause gastroenteritis, urinary tract infections, and neonatal meningitis. In rarer cases, virulent strains are also responsible for hemolytic-uremic syndrome, peritonitis, mastitis, septicemia and Gram-negative pneumonia.[37]

UPEC (uropathogenic E. coli) is one of the main causes of urinary tract infections.[42] It is part of the normal flora in the gut and can be introduced many ways. In particular for females, the direction of wiping after defecation (wiping back to front) can lead to fecal contamination of the urogenital orifices. Anal sex can also introduce this bacteria into the male urethra, and in switching from anal to vaginal intercourse the male can also introduce UPEC to the female urogenital system.[42] For more information, see the databases at the end of the article or UPEC pathogenicity.

In May 2011, one E. coli strain, Escherichia coli O104:H4, has been the subject of a bacterial outbreak that began in Germany. Certain strains of E. coli are a major cause of foodborne illness. The outbreak started when several people in Germany were infected with enterohemorrhagic E. coli (EHEC) bacteria, leading to hemolytic-uremic syndrome (HUS), a medical emergency that requires urgent treatment. The outbreak did not only concern Germany, but 11 other countries, including regions in North America.[43] On 30 June 2011 the German Bundesinstitut für Risikobewertung (BfR) (Federal Institute for Risk Assessment, a federal, fully legal entity under public law of the Federal Republic of Germany, an institute within the German Federal Ministry of Food, Agriculture and Consumer Protection) announced that seeds of fenugreek from Egypt were likely the cause of the EHEC outbreak.[44]

Model organism in life science research

Main article: Escherichia coli (molecular biology)

Role in biotechnology

Because of its long history of laboratory culture and ease of manipulation, E. coli also plays an important role in modern biological engineering and industrial microbiology.[45] The work of Stanley Norman Cohen and Herbert Boyer in E. coli, using plasmids and restriction enzymes to create recombinant DNA, became a foundation of biotechnology.[46]

E. coli is a very versatile host for the production of heterologous proteins,[47] and various protein expression systems have been developed which allow the production of recombinant proteins in E. coli. Researchers can introduce genes into the microbes using plasmids which permit high level expression of protein, and such protein may be mass-produced in industrial fermentation processes. One of the first useful applications of recombinant DNA technology was the manipulation of E. coli to produce human insulin.[48]

Many proteins previously thought difficult or impossible to be expressed in E. coli in folded form have also been successfully expressed in E. coli. For example, proteins with multiple disulphide bonds may be produced in the periplasmic space or in the cytoplasm of mutants rendered sufficiently oxidizing to allow disulphide-bonds to form,[49] while proteins requiring post-translational modification such as glycosylation for stability or function have been expressed using the N-linked glycosylation system of Campylobacter jejuni engineered into E. coli.[50][51][52]

Modified E. coli cells have been used in vaccine development, bioremediation, production of biofuels;[53] lighting, and production of immobilised enzymes.[47][54]

Model organism

E. coli was one of the first organisms to have its genome sequenced; the complete genome of E. coli K12 was published by Science in 1997.[33]

The long-term evolution experiments using E. coli, begun by Richard Lenski in 1988, have allowed direct observation of major evolutionary shifts in the laboratory.[55] In this experiment, one population of E. coli unexpectedly evolved the ability to aerobically metabolize citrate, which is extremely rare in E. coli. As the inability to grow aerobically is normally used as a diagnostic criterion with which to differentiate E. coli from other, closely related bacteria, such as Salmonella, this innovation may mark a speciation event observed in the lab.

By evaluating the possible combination of nanotechnologies with landscape ecology, complex habitat landscapes can be generated with details at the nanoscale.[56] On such synthetic ecosystems, evolutionary experiments with E. coli have been performed to study the spatial biophysics of adaptation in an island biogeography on-chip.

Studies are also being performed into programming E. coli to potentially solve complicated mathematics problems, such as the Hamiltonian path problem.[57]

History

The genera Escherichia and Salmonella diverged around 102 million years ago (credibility interval: 57–176 mya), which coincides with the divergence of their hosts: the former being found in mammals and the latter in birds and reptiles.[58] This was followed by a split of the escherichian ancestor into five species (E. albertii, E. coli, E. fergusonii, E. hermannii and E. vulneris.) The last E. coli ancestor split between 20 and 30 million years ago.[59]

In 1885, a German pediatrician, Theodor Escherich, discovered this organism in the feces of healthy individuals and called it Bacterium coli commune due to the fact it is found in the colon and early classifications of Prokaryotes placed these in a handful of genera based on their shape and motility (at that time Ernst Haeckel's classification of Bacteria in the kingdom Monera was in place[60]).[61] Bacterium coli was the type species of the now invalid genus Bacteriumwhen it was revealed that the former type species ("Bacterium triloculare") was missing.[62] Following a revision of Bacteria it was reclassified as Bacillus coli by Migula in 1895[63] and later reclassified in the newly created genus Escherichia, named after its original discoverer.[64]

The genus belongs in a group of bacteria informally known as "coliforms", and is a member of theEnterobacteriaceae family ("the enterics") of the Gammaproteobacteria.[26]

See also

References

  1. ^ "coli". Oxford English Dictionary (Online ed.). Oxford University Press. (Subscription or participating institution membership required.)
  2. ^ "Escherichia coli". CDC National Center for Emerging and Zoonotic Infectious Diseases. Retrieved 2012-10-02.
  3. ^ Vogt RL, Dippold L (2005). "Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June–July 2002". Public Health Rep. 120 (2): 174–8. PMC 1497708. PMID 15842119.
  4. ^ Bentley R, Meganathan R (1 September 1982). "Biosynthesis of vitamin K (menaquinone) in bacteria". Microbiol. Rev. 46 (3): 241–80. PMC 281544. PMID 6127606.
  5. ^ a b Hudault S, Guignot J, Servin AL (2001). "Escherichia coli strains colonizing the gastrointestinal tract protect germ-free mice against Salmonella typhimurium infection". Gut. 49 (1): 47–55. doi:10.1136/gut.49.1.47. PMC 1728375. PMID 11413110. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) Cite error: The named reference "Hudault" was defined multiple times with different content (see the help page).
  6. ^ Reid G, Howard J, Gan BS (2001). "Can bacterial interference prevent infection?". Trends Microbiol. 9 (9): 424–428. doi:10.1016/S0966-842X(01)02132-1. PMID 11553454. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  7. ^ Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, Gill SR, Nelson KE, Relman DA (2005). "Diversity of the human intestinal microbial flora". Science. 308 (5728): 1635–8. Bibcode:2005Sci...308.1635E. doi:10.1126/science.1110591. PMC 1395357. PMID 15831718. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  8. ^ a b Feng P, Weagant S, Grant, M (2002-09-01). "Enumeration of Escherichia coli and the Coliform Bacteria". Bacteriological Analytical Manual (8th ed.). FDA/Center for Food Safety & Applied Nutrition. Retrieved 2007-01-25.{{cite web}}: CS1 maint: multiple names: authors list (link)
  9. ^ a b Thompson, Andrea (2007-06-04). "E. coli Thrives in Beach Sands". Live Science. Retrieved 2007-12-03.
  10. ^ Ishii S, Sadowsky MJ (2008). "Escherichia coli in the Environment: Implications for Water Quality and Human Health". Microbes Environ. 23 (2): 101–8. doi:10.1264/jsme2.23.101. PMID 21558695.
  11. ^ "E.Coli". Redorbit. Retrieved 27 November 2013.
  12. ^ "Facts about E. coli: dimensions, as discussed in bacteria: Diversity of structure of bacteria: – Britannica Online Encyclopedia". Britannica.com. Retrieved 2011-06-05.
  13. ^ Kubitschek HE (1 January 1990). "Cell volume increase in Escherichia coli after shifts to richer media". J. Bacteriol. 172 (1): 94–101. PMC 208405. PMID 2403552.
  14. ^ Madigan MT, Martinko JM (2006). Brock Biology of microorganisms (11th ed.). Pearson. ISBN 0-13-196893-9.
  15. ^ Fotadar U, Zaveloff P, Terracio L (2005). "Growth of Escherichia coli at elevated temperatures". J. Basic Microbiol. 45 (5): 403–4. doi:10.1002/jobm.200410542. PMID 16187264.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  16. ^ Ingledew WJ, Poole RK (1984). "The respiratory chains of Escherichia coli". Microbiol. Rev. 48 (3): 222–71. PMC 373010. PMID 6387427.
  17. ^ Darnton NC, Turner L, Rojevsky S, Berg HC (2007). "On torque and tumbling in swimming Escherichia coli". J. Bacteriol. 189 (5): 1756–64. doi:10.1128/JB.01501-06. PMC 1855780. PMID 17189361. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  18. ^ Brüssow H, Canchaya C, Hardt WD (2004). "Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion". Microbiol. Mol. Biol. Rev. 68 (3): 560–602. doi:10.1128/MMBR.68.3.560-602.2004. PMC 515249. PMID 15353570. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  19. ^ Krieg, N. R.; Holt, J. G., eds. (1984). Bergey's Manual of Systematic Bacteriology. Vol. 1 (First ed.). Baltimore: The Williams & Wilkins Co. pp. 408–420. ISBN 0-683-04108-8.
  20. ^ a b c Lukjancenko O, Wassenaar TM, Ussery DW (2010). "Comparison of 61 sequenced Escherichia coli genomes". Microb. Ecol. 60 (4): 708–20. doi:10.1007/s00248-010-9717-3. PMC 2974192. PMID 20623278. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) Cite error: The named reference "comparison" was defined multiple times with different content (see the help page).
  21. ^ Lan R, Reeves PR (2002). "Escherichia coli in disguise: molecular origins of Shigella". Microbes Infect. 4 (11): 1125–32. doi:10.1016/S1286-4579(02)01637-4. PMID 12361912. {{cite journal}}: Unknown parameter |month= ignored (help)
  22. ^ Orskov I, Orskov F, Jann B, Jann K (1977). "Serology, chemistry, and genetics of O and K antigens of Escherichia coli". Bacteriol Rev. 41 (3): 667–710. PMC 414020. PMID 334154. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  23. ^ Stenutz, R.; Weintraub, A.; Widmalm, G. (2006), "The structures of Escherichia coli O-polysaccharide antigens", FEMS Microbiol Rev, doi:10.1111/j.1574-6976.2006.00016.x
  24. ^ Lawrence JG, Ochman H (1998). "Molecular archaeology of the Escherichia coli genome". Proc. Natl. Acad. Sci. U.S.A. 95 (16): 9413–7. Bibcode:1998PNAS...95.9413L. doi:10.1073/pnas.95.16.9413. PMC 21352. PMID 9689094. {{cite journal}}: Unknown parameter |month= ignored (help)
  25. ^ a b Nataro JP, Kaper JB (1998). "Diarrheagenic Escherichia coli". Clin. Microbiol. Rev. 11 (1): 142–201. PMC 121379. PMID 9457432. {{cite journal}}: Unknown parameter |month= ignored (help)
  26. ^ a b Brenner, Don J.; Krieg, Noel R.; Staley, James T. (July 26, 2005) [1984 (Williams & Wilkins)]. George M. Garrity (ed.). The Gammaproteobacteria. Bergey's Manual of Systematic Bacteriology. Vol. 2B (2nd ed.). New York: Springer. p. 1108. ISBN 978-0-387-24144-9. British Library no. GBA561951.
  27. ^ Discussion of nomenclature of Enterobacteriaceae in LPSN; Parte, Aidan C.; Sardà Carbasse, Joaquim; Meier-Kolthoff, Jan P.; Reimer, Lorenz C.; Göker, Markus (1 November 2020). "List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ". International Journal of Systematic and Evolutionary Microbiology. 70 (11): 5607–5612. doi:10.1099/ijsem.0.004332.
  28. ^ International Bulletin of Bacteriological Nomenclature and Taxonomy 8:73–74 (1958)
  29. ^ http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=11775&Template=bacteria
  30. ^ "Escherichia". bacterio.cict.fr.
  31. ^ "Escherichia coli (Migula 1895) Castellani and Chalmers 1919". JCM Catalogue.
  32. ^ Brzuszkiewicz E, Thürmer A, Schuldes J, Leimbach A, Liesegang H, Meyer FD, Boelter J, Petersen H, Gottschalk G, Daniel R (2011). "Genome sequence analyses of two isolates from the recent Escherichia coli outbreak in Germany reveal the emergence of a new pathotype: Entero-Aggregative-Haemorrhagic Escherichia coli (EAHEC)". Arch. Microbiol. 193 (12): 883–91. doi:10.1007/s00203-011-0725-6. PMC 3219860. PMID 21713444. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  33. ^ a b Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997). "The complete genome sequence of Escherichia coli K-12". Science. 277 (5331): 1453–62. doi:10.1126/science.277.5331.1453. PMID 9278503. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  34. ^ Zhaxybayeva O, Doolittle WF (2011). "Lateral gene transfer". Curr. Biol. 21 (7): R242–6. doi:10.1016/j.cub.2011.01.045. PMID 21481756. {{cite journal}}: Unknown parameter |month= ignored (help)
  35. ^ Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12". Genome Res. 16 (5): 686–91. doi:10.1101/gr.4527806. PMC 1457052. PMID 16606699. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  36. ^ Hu P, Janga SC, Babu M, Díaz-Mejía JJ, Butland G, Yang W, Pogoutse O, Guo X, Phanse S, Wong P, Chandran S, Christopoulos C, Nazarians-Armavil A, Nasseri NK, Musso G, Ali M, Nazemof N, Eroukova V, Golshani A, Paccanaro A, Greenblatt JF, Moreno-Hagelsieb G, Emili A (2009). "Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins". PLoS Biol. 7 (4): e96. doi:10.1371/journal.pbio.1000096. PMC 2672614. PMID 19402753. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  37. ^ a b Todar, K. "Pathogenic E. coli". Online Textbook of Bacteriology. University of Wisconsin–Madison Department of Bacteriology. Retrieved 2007-11-30. {{cite web}}: Cite has empty unknown parameter: |coauthors= (help)
  38. ^ Evans Jr., Doyle J. "Escherichia Coli". Medical Microbiology, 4th edition. The University of Texas Medical Branch at Galveston. Archived from the original on 2007-11-02. Retrieved 2007-12-02. {{cite web}}: Unknown parameter |coauthors= ignored (|author= suggested) (help)
  39. ^ Lodinová-Zádníková R, Cukrowska B, Tlaskalova-Hogenova H. Oral administration of probiotic Escherichia coli after birth reduces frequency of allergies and repeated infections later in life (after 10 and 20 years). Int Arch Allergy Immunol. 2003 Jul;131(3):209-11. PubMed PMID 12876412.
  40. ^ Grozdanov L, Raasch C, Schulze J, Sonnenborn U, Gottschalk G, Hacker J, Dobrindt U (2004). "Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917". J. Bacteriol. 186 (16): 5432–41. doi:10.1128/JB.186.16.5432-5441.2004. PMC 490877. PMID 15292145. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  41. ^ Kamada N, Inoue N, Hisamatsu T, Okamoto S, Matsuoka K, Sato T, Chinen H, Hong KS, Yamada T, Suzuki Y, Suzuki T, Watanabe N, Tsuchimoto K, Hibi T (2005). "Nonpathogenic Escherichia coli strain Nissle1917 prevents murine acute and chronic colitis". Inflamm. Bowel Dis. 11 (5): 455–63. doi:10.1097/01.MIB.0000158158.55955.de. PMID 15867585. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  42. ^ a b "Uropathogenic Escherichia coli: The Pre-Eminent Urinary Tract Infection Pathogen". Nova publishers. Retrieved 27 November 2013.
  43. ^ Enterohemorrhagic Escherichia Coli (EHEC) outbreak in Germany
  44. ^ "Samen von Bockshornklee mit hoher Wahrscheinlichkeit für EHEC O104:H4 Ausbruch verantwortlich in English: Fenugreek seeds with high probability for EHEC O104: H4 responsible outbreak" (PDF) (in German). Bundesinstitut für Risikobewertung (BfR) in English: Federal Institute for Risk Assessment. 30 June 2011. Retrieved 17 July 2011. {{cite web}}: Italic or bold markup not allowed in: |publisher= (help)
  45. ^ Lee SY (1996). "High cell-density culture of Escherichia coli". Trends Biotechnol. 14 (3): 98–105. doi:10.1016/0167-7799(96)80930-9. PMID 8867291.
  46. ^ Russo E (2003). "The birth of biotechnology". Nature. 421 (6921): 456–457. Bibcode:2003Natur.421..456R. doi:10.1038/nj6921-456a. PMID 12540923. {{cite journal}}: Unknown parameter |month= ignored (help)
  47. ^ a b Cornelis P (2000). "Expressing genes in different Escherichia coli compartments". Curr. Opin. Biotechnol. 11 (5): 450–454. doi:10.1016/S0958-1669(00)00131-2. PMID 11024362.
  48. ^ Tof, Ilanit (1994). "Recombinant DNA Technology in the Synthesis of Human Insulin". Little Tree Pty. Ltd. Retrieved 2007-11-30. {{cite web}}: Cite has empty unknown parameter: |coauthors= (help)
  49. ^ Bessette PH, Aslund F, Beckwith J, Georgiou G (1999). "Efficient folding of proteins with multiple disulfide bonds in the Escherichia coli cytoplasm". Proc. Natl. Acad. Sci. U.S.A. 96 (24): 13703–8. Bibcode:1999PNAS...9613703B. doi:10.1073/pnas.96.24.13703. PMC 24128. PMID 10570136. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  50. ^ Ihssen J, Kowarik M, Dilettoso S, Tanner C, Wacker M, Thöny-Meyer L. (2010). "Production of glycoprotein vaccines in Escherichia coli". Microbial Cell Factories. 9 (61): 494–7. PMC 2927510. PMID 20701771.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  51. ^ Wacker M, Linton D, Hitchen PG, Nita-Lazar M, Haslam SM, North SJ, Panico M, Morris HR, Dell A, Wren BW, Aebi M (2002). "N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli". Science. 298 (5599): 1790–1793. doi:10.1126/science.298.5599.1790. PMID 12459590.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  52. ^ Huang CJ, Lin H, Yang X. (2012). "Industrial production of recombinant therapeutics in Escherichia coli and its recent advancements". J Ind Microbiol Biotechnol. 39 (3): 383–99. doi:10.1007/s10295-011-1082-9. PMID 22252444.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  53. ^ Summers, Rebecca (24 April 2013) Bacteria churn out first ever petrol-like biofuel New Scientist, Retrieved 27 April 2013
  54. ^ Nic Halverson (August 15, 2013). "Bacteria-Powered Light Bulb Is Electricity-Free".
  55. ^ Bacteria make major evolutionary shift in the lab New Scientist
  56. ^ Keymer JE, Galajda P, Muldoon C, Park S, Austin RH (2006). "Bacterial metapopulations in nanofabricated landscapes". Proc. Natl. Acad. Sci. U.S.A. 103 (46): 17290–5. Bibcode:2006PNAS..10317290K. doi:10.1073/pnas.0607971103. PMC 1635019. PMID 17090676. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  57. ^ "Solving a Hamiltonian Path Problem with a bacterial computer". J Biol Eng. 2009; 3: 11. July 24, 2009.
  58. ^ Battistuzzi FU, Feijao A, Hedges SB (2004). "A genomic timescale of prokaryote evolution: insights into the origin of methanogenesis, phototrophy, and the colonization of land". BMC Evol. Biol. 4: 44. doi:10.1186/1471-2148-4-44. PMC 533871. PMID 15535883. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link) CS1 maint: unflagged free DOI (link)
  59. ^ Lecointre G, Rachdi L, Darlu P, Denamur E (1998). "Escherichia coli molecular phylogeny using the incongruence length difference test". Mol. Biol. Evol. 15 (12): 1685–95. PMID 9866203. {{cite journal}}: Unknown parameter |month= ignored (help)CS1 maint: multiple names: authors list (link)
  60. ^ Haeckel, Ernst (1867). Generelle Morphologie der Organismen. Reimer, Berlin. ISBN 1-144-00186-2.
  61. ^ Escherich T (1885). "Die Darmbakterien des Neugeborenen und Säuglinge". Fortschr. Med. 3: 515–522.
  62. ^ Breed RS, Conn HJ (1936). "The Status of the Generic Term Bacterium Ehrenberg 1828". J. Bacteriol. 31 (5): 517–8. PMC 543738. PMID 16559906. {{cite journal}}: Unknown parameter |month= ignored (help)
  63. ^ MIGULA (W.): Bacteriaceae (Stabchenbacterien). In: A. ENGLER and K. PRANTL (eds): Die Naturlichen Pfanzenfamilien, W. Engelmann, Leipzig, Teil I, Abteilung Ia, 1895, pp. 20–30.
  64. ^ CASTELLANI (A.) and CHALMERS (A.J.): Manual of Tropical Medicine, 3rd ed., Williams Wood and Co., New York, 1919.

External links

Wikispecies has information related to Escherichia coli.
Wikimedia Commons has media related to Escherichia coli.

Databases

  • EcoSal Continually updated Web resource based on the classic ASM Press publication Escherichia coli and Salmonella: Cellular and Molecular Biology
  • Uropathogenic Escherichia coli (UPEC)
  • RegulonDB RegulonDB is a model of the complex regulation of transcription initiation or regulatory network of the cell E. coli K-12.
  • ECODAB The structure of the O-antigens that form the basis of the serological classification of E. coli
  • 2DBase 2D-PAGE Database of Escherichia coli University of Bielefeld – Fermentation Engineering Group (AGFT)
  • 5S rRNA Database Information on nucleotide sequences of 5S rRNAs and their genes
  • ACLAME A CLAssification of Mobile genetic Elements
  • AlignACE Matrices that search for additional binding sites in the E. coli genomic sequence
  • ArrayExpress Database of functional genomics experiments
  • ASAP Comprehensive genome information for several enteric bacteria with community annotation
  • Bacteriome E. coli DNA-Binding Site Matrices Applied to the Complete E. coli K-12 Genome
  • BioGPS Gene portal hub
  • BRENDA Comprehensive Enzyme Information System
  • BSGI Bacterial Structural Genomics Initiative
  • CATH Protein Structure Classification
  • CBS Genome Atlas
  • CDD Conserved Domain Database
  • CIBEX Center for Information Biology Gene Expression Database
  • COGs
  • Coli Genetic Stock Center Strains and genetic information on E. coli K-12
  • coliBASE
  • EcoCyc – literature-based curation of the entire genome, and of transcriptional regulation, transporters, and metabolic pathways
  • PortEco (formerly EcoliHub) – NIH-funded comprehensive data resource for E. coli K-12 and its phage, plasmids, and mobile genetic elements
  • EcoliWiki is the community annotation component of PortEco
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